Restriction map for
pFN21AB8780
(Circular) (Six-base) MAPSORT of: pFN21AB8780.seq Check: 6581 from: 1 to: 5482
pFN21AB8780 5482 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3292 278
Size: 53 83 186 936 1756 2468
Fragments arranged by size:
2468 1756 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2825 1937
Size: 888 4594
Acc65I G'GTAC_C
Cuts at: 2803 2803
Size: 5482
AclI AA'CG_TT
Cuts at: 1563 3609 3982 1563
Size: 2046 373 3063
Fragments arranged by size:
3063 2046 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2087 2132 3545 4929 5197 1632
Size: 455 45 1413 1384 268 1917
Fragments arranged by size:
1917 1413 1384 455 268 45
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5285
AflIII A'CryG_T
Cuts at: 1203 4381 5358 1203
Size: 3178 977 1327
Fragments arranged by size:
3178 1327 977
AgeI A'CCGG_T
Cuts at: 4313 4313
Size: 5482
AhdI GACnn_n'nnGTC
Cuts at: 4211 4211
Size: 5482
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5482
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5482
AlwNI CAG_nnn'CTG
Cuts at: 2065 2356 3225 4797 2065
Size: 291 869 1572 2750
Fragments arranged by size:
2750 1572 869 291
ApaI G_GGCC'C
Cuts at: 2213 2762 2213
Size: 549 4933
ApaLI G'TGCA_C
Cuts at: 3539 4695 3539
Size: 1156 4326
ApoI r'AATT_y
Cuts at: 1493 2791 3017 3169 5278 1493
Size: 1298 226 152 2109 1697
Fragments arranged by size:
2109 1697 1298 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4036 160
Size: 872 3004 1606
Fragments arranged by size:
3004 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5482
AvaI C'yCGr_G
Cuts at: 1870 1943 2338 2807 1870
Size: 73 395 469 4545
Fragments arranged by size:
4545 469 395 73
BamHI G'GATC_C
Cuts at: 2812 3179 2812
Size: 367 5115
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2803 4258 618
Size: 325 217 627 1016 1455 1842
Fragments arranged by size:
1842 1455 1016 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2213 2762 2801 1391
Size: 822 549 39 4072
Fragments arranged by size:
4072 822 549 39
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2770 928
Size: 971 871 3640
Fragments arranged by size:
3640 971 871
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3673 3707 1903
Size: 34 1736 34 3678
Fragments arranged by size:
3678 1736 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3374 4584 3374
Size: 1210 4272
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5482
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2588 4093 136
Size: 107 122 71 631 605 916 1505 1525
Fragments arranged by size:
1525 1505 916 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5477 5477
Size: 5482
BlpI GC'TnA_GC
Cuts at: 2411 2899 2411
Size: 488 4994
Bme1580I G_kGCm'C
Cuts at: 1790 2213 2762 3543 4699 1790
Size: 423 549 781 1156 2573
Fragments arranged by size:
2573 1156 781 549 423
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5482
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2452 2626 3213
Size: 416 53 83 399 24 1035 174 587
Cuts at: 3213 4171 442
Size: 958 1753
Fragments arranged by size:
1753 1035 958 587 416 399 174 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2237 1056
Size: 1181 4301
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1998 2114 2189 2294 2396 2548 2672 4142
Size: 759 116 75 105 102 152 124 1470
Cuts at: 4142 1239
Size: 2579
Fragments arranged by size:
2579 1470 759 152 124 116 105 102
75
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3607 4472 4770 3607
Size: 865 298 4319
Fragments arranged by size:
4319 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2388 2529 4145 882
Size: 617 889 141 1616 2219
Fragments arranged by size:
2219 1616 889 617 141
BsaAI yAC'GTr
Cuts at: 493 1204 3273 493
Size: 711 2069 2702
Fragments arranged by size:
2702 2069 711
BsaBI GATnn'nnATC
Cuts at: 5476 5476
Size: 5482
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3289 3671 4306 275
Size: 53 83 186 936 1756 382 635 1451
Fragments arranged by size:
1756 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3915 4313 4587 4734 5145 3915
Size: 398 274 147 411 4252
Fragments arranged by size:
4252 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5257
Fragments arranged by size:
5257 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2669 1186
Size: 1483 3999
BseYI C'CCAG_C
Cuts at: 2058 2360 2558 2592 2630 2694 4685 5181 2058
Size: 302 198 34 38 64 1991 496 2359
Fragments arranged by size:
2359 1991 496 302 198 64 38 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4909
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2932 3693 4721 1540
Size: 275 186 931 761 1028 2301
Fragments arranged by size:
2301 1028 931 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2244 3543 3628 4699 2244
Size: 1299 85 1071 3027
Fragments arranged by size:
3027 1299 1071 85
BsmI GAATG_Cn'
Cuts at: 2993 3086 2993
Size: 93 5389
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2213 2244 2762 2801 3543 3628 4699
Size: 399 423 31 518 39 742 85 1071
Cuts at: 4699 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 518 423 399 85 39
31
BspEI T'CCGG_A
Cuts at: 5145 5145
Size: 5482
BspHI T'CATG_A
Cuts at: 3371 5101 3371
Size: 1730 3752
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2391 2731 2837 844
Size: 1547 340 106 3489
Fragments arranged by size:
3489 1547 340 106
BsrBI CCG'CTC
Cuts at: 1394 1823 2422 3369 1394
Size: 429 599 947 3507
Fragments arranged by size:
3507 947 599 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3977 4151 65
Size: 1183 2729 174 1396
Fragments arranged by size:
2729 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2664 4126 4313 1700
Size: 110 854 1462 187 2869
Fragments arranged by size:
2869 1462 854 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5482
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5482
BssSI C'ACGA_G
Cuts at: 3542 4554 3542
Size: 1012 4470
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5482
BstBI TT'CG_AA
Cuts at: 4286 4286
Size: 5482
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5482
BstXI CCAn_nnnn'nTGG
Cuts at: 2616 2616
Size: 5482
BstYI r'GATC_y
Cuts at: 1972 2812 3179 3564 3581 5033 5119 5131 5477
Size: 840 367 385 17 1452 86 12 346
Cuts at: 5477 1972
Size: 1977
Fragments arranged by size:
1977 1452 840 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3991
Fragments arranged by size:
3991 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4743
Fragments arranged by size:
4743 683 56
BtsI GCAGTG_nn'
Cuts at: 2357 2681 2998 3792 3812 2357
Size: 324 317 794 20 4027
Fragments arranged by size:
4027 794 324 317 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5482
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5447
DraI TTT'AAA
Cuts at: 2786 2786
Size: 5482
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4489 809
Size: 507 3173 1802
Fragments arranged by size:
3173 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2283 2331 2929
Size: 54 1425 50 275 18 453 48 598
Cuts at: 2929 3818 5447 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 598 453 275 54 50
48 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2929 1537
Size: 275 1117 4090
Fragments arranged by size:
4090 1117 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2773 3412 1353
Size: 1420 639 3423
Fragments arranged by size:
3423 1420 639
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2055 2750 4069 4443 4589 387
Size: 1064 138 466 695 1319 374 146 1280
Fragments arranged by size:
1319 1280 1064 695 466 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 1998 2087 2114 2132 2189 2294 2396
Size: 393 366 89 27 18 57 105 102
Cuts at: 2396 2548 2672 3545 4142 4929 5197 1239
Size: 152 124 873 597 787 268 1524
Fragments arranged by size:
1524 873 787 597 393 366 268 152
124 105 102 89 57 27 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2654 2729 2654
Size: 75 5407
EcoO109I rG'GnC_Cy
Cuts at: 2573 2698 2759 2573
Size: 125 61 5296
Fragments arranged by size:
5296 125 61
EcoRI G'AATT_C
Cuts at: 2791 3169 2791
Size: 378 5104
FspI TGC'GCA
Cuts at: 3988 3988
Size: 5482
HaeII r_GCGC'y
Cuts at: 1678 4629 1678
Size: 2951 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2034 2066 4123 4155 4197
Size: 32 135 32 462 32 2057 32 42
Cuts at: 4197 4229 1373
Size: 32 2626
Fragments arranged by size:
2626 2057 462 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2826 3072 669
Size: 988 281 888 246 3079
Fragments arranged by size:
3079 988 888 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5482
HpaI GTT'AAC
Cuts at: 3072 3072
Size: 5482
KpnI G_GTAC'C
Cuts at: 2807 2807
Size: 5482
MfeI C'AATT_G
Cuts at: 3081 3081
Size: 5482
MluI A'CGCG_T
Cuts at: 5358 5358
Size: 5482
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2277 4596 4780 1305
Size: 972 2319 184 2007
Fragments arranged by size:
2319 2007 972 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2285 2333 10
Size: 54 1425 343 453 48 3159
Fragments arranged by size:
3159 1425 453 343 54 48
MslI CAynn'nnrTG
Cuts at: 518 1166 3440 3799 3958 518
Size: 648 2274 359 159 2042
Fragments arranged by size:
2274 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2018 2062 2215 2347 2527 2898 3575 4723
Size: 412 44 153 132 180 371 677 1148
Cuts at: 4723 4968 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 412 371 245 180 153
132 44
NaeI GCC'GGC
Cuts at: 2666 2666
Size: 5482
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3991
Fragments arranged by size:
3991 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5482
NgoMIV G'CCGG_C
Cuts at: 2664 2664
Size: 5482
NheI G'CTAG_C
Cuts at: 1052 2233 1052
Size: 1181 4301
NotI GC'GGCC_GC
Cuts at: 2929 2929
Size: 5482
NspI r_CATG'y
Cuts at: 1132 1229 2840 4385 1132
Size: 97 1611 1545 2229
Fragments arranged by size:
2229 1611 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2598 1348
Size: 1250 4232
PciI A'CATG_T
Cuts at: 4381 4381
Size: 5482
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5482
PfoI T'CCnGG_A
Cuts at: 2454 2649 2673 2454
Size: 195 24 5263
Fragments arranged by size:
5263 195 24
PmeI GTTT'AAAC
Cuts at: 2786 2786
Size: 5482
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3551 3583 5064 5096 3551
Size: 32 1481 32 3937
Fragments arranged by size:
3937 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2698 2698
Size: 5482
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5482
PsiI TTA'TAA
Cuts at: 3052 3052
Size: 5482
PspOMI G'GGCC_C
Cuts at: 2209 2758 2209
Size: 549 4933
PstI C_TGCA'G
Cuts at: 830 2049 2352 2452 2688 2834 830
Size: 1219 303 100 236 146 3478
Fragments arranged by size:
3478 1219 303 236 146 100
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5482
PvuII CAG'CTG
Cuts at: 2062 2347 2527 2062
Size: 285 180 5017
Fragments arranged by size:
5017 285 180
SalI G'TCGA_C
Cuts at: 1936 2824 1936
Size: 888 4594
SbfI CC_TGCA'GG
Cuts at: 2834 2834
Size: 5482
ScaI AGT'ACT
Cuts at: 3730 5442 3730
Size: 1712 3770
SfcI C'TryA_G
Cuts at: 826 1045 2045 2348 2448 2684 2830 3965 4646
Size: 219 1000 303 100 236 146 1135 681
Cuts at: 4646 4837 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1000 681 303 236 219 191
146 100
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5482
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5482
SmaI CCC'GGG
Cuts at: 1872 2340 2809 1872
Size: 468 469 4545
Fragments arranged by size:
4545 469 468
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3586 4487 4749 820
Size: 197 926 1643 901 262 1553
Fragments arranged by size:
1643 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3273 493
Size: 2780 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5482
SphI G_CATG'C
Cuts at: 1132 2840 1132
Size: 1708 3774
SspI AAT'ATT
Cuts at: 5 52 3406 5
Size: 47 3354 2081
Fragments arranged by size:
3354 2081 47
StuI AGG'CCT
Cuts at: 1517 2660 1517
Size: 1143 4339
StyI C'CwwG_G
Cuts at: 513 1065 2004 2921 513
Size: 552 939 917 3074
Fragments arranged by size:
3074 939 917 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2216 2590 3509 3526 3679 3864 4309
Size: 1255 636 374 919 17 153 185 445
Cuts at: 4309 325
Size: 1498
Fragments arranged by size:
1498 1255 919 636 445 374 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3728 5440 96
Size: 274 80 33 51 1443 1751 1712 138
Fragments arranged by size:
1751 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5442 5474 5442
Size: 32 5450
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5482
XbaI T'CTAG_A
Cuts at: 2818 2818
Size: 5482
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2722 2722
Size: 5482
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5482
XmaI C'CCGG_G
Cuts at: 1870 2338 2807 1870
Size: 468 469 4545
Fragments arranged by size:
4545 469 468
XmnI GAAnn'nnTTC
Cuts at: 3611 3611
Size: 5482
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3290 276
Size: 53 83 186 936 1756 2468
Fragments arranged by size:
2468 1756 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI
NsiI PacI PmlI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI