Restriction map for
pFN21AB8779
(Circular) (Six-base) MAPSORT of: pFN21AB8779.seq Check: 8416 from: 1 to: 5482
pFN21AB8779 5482 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3292 278
Size: 53 83 186 936 1756 2468
Fragments arranged by size:
2468 1756 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2016 2825 1937
Size: 79 809 4594
Fragments arranged by size:
4594 809 79
Acc65I G'GTAC_C
Cuts at: 2803 2803
Size: 5482
AclI AA'CG_TT
Cuts at: 1563 3609 3982 1563
Size: 2046 373 3063
Fragments arranged by size:
3063 2046 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2432 3545 4929 5197 1632
Size: 800 1113 1384 268 1917
Fragments arranged by size:
1917 1384 1113 800 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5285
AflIII A'CryG_T
Cuts at: 1203 4381 5358 1203
Size: 3178 977 1327
Fragments arranged by size:
3178 1327 977
AgeI A'CCGG_T
Cuts at: 4313 4313
Size: 5482
AhdI GACnn_n'nnGTC
Cuts at: 4211 4211
Size: 5482
AleI CACnn'nnGTG
Cuts at: 1166 2651 1166
Size: 1485 3997
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2412 1184
Size: 1228 4254
AlwNI CAG_nnn'CTG
Cuts at: 2356 3225 4797 2356
Size: 869 1572 3041
Fragments arranged by size:
3041 1572 869
ApaLI G'TGCA_C
Cuts at: 2387 3539 4695 2387
Size: 1152 1156 3174
Fragments arranged by size:
3174 1156 1152
ApoI r'AATT_y
Cuts at: 1493 2250 2791 3017 3169 5278 1493
Size: 757 541 226 152 2109 1697
Fragments arranged by size:
2109 1697 757 541 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4036 160
Size: 872 3004 1606
Fragments arranged by size:
3004 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5482
AvaI C'yCGr_G
Cuts at: 1870 1943 2807 1870
Size: 73 864 4545
Fragments arranged by size:
4545 864 73
BamHI G'GATC_C
Cuts at: 2812 3179 2812
Size: 367 5115
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2803 4258 618
Size: 325 217 627 1016 1455 1842
Fragments arranged by size:
1842 1455 1016 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2801 1391
Size: 1410 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4511
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2303 2337 3673 3707 1903
Size: 34 366 34 1336 34 3678
Fragments arranged by size:
3678 1336 366 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3374 4584 3374
Size: 1210 4272
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5482
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2737 4093 136
Size: 107 122 71 631 605 1065 1356 1525
Fragments arranged by size:
1525 1356 1065 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2185 5477 2185
Size: 3292 2190
BlpI GC'TnA_GC
Cuts at: 2899 2899
Size: 5482
Bme1580I G_kGCm'C
Cuts at: 1790 2391 3543 4699 1790
Size: 601 1152 1156 2573
Fragments arranged by size:
2573 1156 1152 601
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5482
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3213 4171 442
Size: 416 53 83 399 24 1796 958 1753
Fragments arranged by size:
1796 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5482
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4142 1239
Size: 2903 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2153 3607 4472 4770 2153
Size: 1454 865 298 2865
Fragments arranged by size:
2865 1454 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4145 882
Size: 617 2646 2219
Fragments arranged by size:
2646 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3273 493
Size: 711 2069 2702
Fragments arranged by size:
2702 2069 711
BsaBI GATnn'nnATC
Cuts at: 5476 5476
Size: 5482
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2486 3289 3671 4306
Size: 53 83 186 936 953 803 382 635
Cuts at: 4306 275
Size: 1451
Fragments arranged by size:
1451 953 936 803 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3915 4313 4587 4734 5145 3915
Size: 398 274 147 411 4252
Fragments arranged by size:
4252 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5257
Fragments arranged by size:
5257 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2042 2491 1186
Size: 856 449 4177
Fragments arranged by size:
4177 856 449
BseYI C'CCAG_C
Cuts at: 4685 5181 4685
Size: 496 4986
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4909
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2932 3693 4721 1540
Size: 275 186 931 761 1028 2301
Fragments arranged by size:
2301 1028 931 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2391 3543 3628 4699 2391
Size: 1152 85 1071 3174
Fragments arranged by size:
3174 1152 1071 85
BsmI GAATG_Cn'
Cuts at: 2993 3086 2993
Size: 93 5389
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2391 2801 3543 3628 4699 1391
Size: 399 601 410 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 601 410 399 85
BspEI T'CCGG_A
Cuts at: 5145 5145
Size: 5482
BspHI T'CATG_A
Cuts at: 2494 3371 5101 2494
Size: 877 1730 2875
Fragments arranged by size:
2875 1730 877
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2837 844
Size: 1993 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3369 1394
Size: 429 1546 3507
Fragments arranged by size:
3507 1546 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2164 3977 4151 65
Size: 1183 916 1813 174 1396
Fragments arranged by size:
1813 1396 1183 916 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4126 4313 1700
Size: 110 2316 187 2869
Fragments arranged by size:
2869 2316 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5482
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5482
BssSI C'ACGA_G
Cuts at: 3542 4554 3542
Size: 1012 4470
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5482
BstBI TT'CG_AA
Cuts at: 4286 4286
Size: 5482
BstEII G'GTnAC_C
Cuts at: 1184 2626 1184
Size: 1442 4040
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5482
BstYI r'GATC_y
Cuts at: 1972 2185 2812 3179 3564 3581 5033 5119 5131
Size: 213 627 367 385 17 1452 86 12
Cuts at: 5131 5477 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 627 385 367 346 213 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4930
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4743
Fragments arranged by size:
4743 683 56
BtsI GCAGTG_nn'
Cuts at: 2446 2998 3792 3812 2446
Size: 552 794 20 4116
Fragments arranged by size:
4116 794 552 20
ClaI AT'CG_AT
Cuts at: 1554 2313 1554
Size: 759 4723
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5447
DraI TTT'AAA
Cuts at: 2142 2786 2142
Size: 644 4838
DraIII CAC_nnn'GTG
Cuts at: 2512 2512
Size: 5482
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4489 809
Size: 507 3173 1802
Fragments arranged by size:
3173 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2929 3818 5447
Size: 54 1425 50 275 18 1099 889 1629
Cuts at: 5447 8
Size: 43
Fragments arranged by size:
1629 1425 1099 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2929 1537
Size: 275 1117 4090
Fragments arranged by size:
4090 1117 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3412 1353
Size: 2059 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4069 4443 4589 387
Size: 1064 138 2480 374 146 1280
Fragments arranged by size:
2480 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2432 3545 4142 4929 5197 1239
Size: 393 800 1113 597 787 268 1524
Fragments arranged by size:
1524 1113 800 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2260 2260
Size: 5482
EcoRI G'AATT_C
Cuts at: 2791 3169 2791
Size: 378 5104
FspI TGC'GCA
Cuts at: 3988 3988
Size: 5482
HaeII r_GCGC'y
Cuts at: 1678 4629 1678
Size: 2951 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2544 2576 2671 2703 4123
Size: 32 135 32 972 32 95 32 1420
Cuts at: 4123 4155 4197 4229 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1420 972 135 95 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2028 2826 3072 669
Size: 988 281 90 798 246 3079
Fragments arranged by size:
3079 988 798 281 246 90
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5482
HpaI GTT'AAC
Cuts at: 2028 3072 2028
Size: 1044 4438
KpnI G_GTAC'C
Cuts at: 2807 2807
Size: 5482
MfeI C'AATT_G
Cuts at: 3081 3081
Size: 5482
MluI A'CGCG_T
Cuts at: 5358 5358
Size: 5482
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2391 2514 4596 4780 1305
Size: 1086 123 2082 184 2007
Fragments arranged by size:
2082 2007 1086 184 123
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3660
Fragments arranged by size:
3660 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2394 2651 3440 3799 3958 518
Size: 648 1228 257 789 359 159 2042
Fragments arranged by size:
2042 1228 789 648 359 257 159
MspA1I CmG'CkG
Cuts at: 1606 2898 3575 4723 4968 1606
Size: 1292 677 1148 245 2120
Fragments arranged by size:
2120 1292 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4930
NdeI CA'TA_TG
Cuts at: 387 2662 387
Size: 2275 3207
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5482
NotI GC'GGCC_GC
Cuts at: 2929 2929
Size: 5482
NspI r_CATG'y
Cuts at: 1132 1229 2324 2546 2840 4385 1132
Size: 97 1095 222 294 1545 2229
Fragments arranged by size:
2229 1545 1095 294 222 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5482
PciI A'CATG_T
Cuts at: 4381 4381
Size: 5482
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5482
PmeI GTTT'AAAC
Cuts at: 2786 2786
Size: 5482
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3551 3583 5064 5096 3551
Size: 32 1481 32 3937
Fragments arranged by size:
3937 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2260 2260
Size: 5482
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5482
PsiI TTA'TAA
Cuts at: 3052 3052
Size: 5482
PstI C_TGCA'G
Cuts at: 830 2834 830
Size: 2004 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5482
SalI G'TCGA_C
Cuts at: 1936 2824 1936
Size: 888 4594
SbfI CC_TGCA'GG
Cuts at: 2834 2834
Size: 5482
ScaI AGT'ACT
Cuts at: 3730 5442 3730
Size: 1712 3770
SfcI C'TryA_G
Cuts at: 826 1045 2152 2462 2830 3965 4646 4837 826
Size: 219 1107 310 368 1135 681 191 1471
Fragments arranged by size:
1471 1135 1107 681 368 310 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5482
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5482
SmaI CCC'GGG
Cuts at: 1872 2809 1872
Size: 937 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2168 3586 4487 4749 820
Size: 197 926 225 1418 901 262 1553
Fragments arranged by size:
1553 1418 926 901 262 225 197
SnaBI TAC'GTA
Cuts at: 493 3273 493
Size: 2780 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5482
SphI G_CATG'C
Cuts at: 1132 2324 2840 1132
Size: 1192 516 3774
Fragments arranged by size:
3774 1192 516
SspI AAT'ATT
Cuts at: 5 52 3406 5
Size: 47 3354 2081
Fragments arranged by size:
3354 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5482
StyI C'CwwG_G
Cuts at: 513 1065 2109 2921 513
Size: 552 1044 812 3074
Fragments arranged by size:
3074 1044 812 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3509 3526 3679 3864 4309 325
Size: 1255 1929 17 153 185 445 1498
Fragments arranged by size:
1929 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2456 3728 5440
Size: 274 80 33 51 1443 479 1272 1712
Cuts at: 5440 96
Size: 138
Fragments arranged by size:
1712 1443 1272 479 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2224 2256 2368 2400 5442 5474 2224
Size: 32 112 32 3042 32 2232
Fragments arranged by size:
3042 2232 112 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5482
XbaI T'CTAG_A
Cuts at: 2818 2818
Size: 5482
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5482
XmaI C'CCGG_G
Cuts at: 1870 2807 1870
Size: 937 4545
XmnI GAAnn'nnTTC
Cuts at: 3611 3611
Size: 5482
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3290 276
Size: 53 83 186 936 1756 2468
Fragments arranged by size:
2468 1756 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI