Restriction map for
pFN21AB8775
(Circular) (Six-base) MAPSORT of: pFN21AB8775.seq Check: 7830 from: 1 to: 5446
pFN21AB8775 5446 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3256 278
Size: 53 83 186 936 1720 2468
Fragments arranged by size:
2468 1720 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2789 1937
Size: 852 4594
Acc65I G'GTAC_C
Cuts at: 2767 2767
Size: 5446
AclI AA'CG_TT
Cuts at: 1563 3573 3946 1563
Size: 2010 373 3063
Fragments arranged by size:
3063 2010 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2043 2569 3509 4893 5161 1632
Size: 411 526 940 1384 268 1917
Fragments arranged by size:
1917 1384 940 526 411 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5249
AflIII A'CryG_T
Cuts at: 1203 2373 4345 5322 1203
Size: 1170 1972 977 1327
Fragments arranged by size:
1972 1327 1170 977
AgeI A'CCGG_T
Cuts at: 4277 4277
Size: 5446
AhdI GACnn_n'nnGTC
Cuts at: 4175 4175
Size: 5446
AleI CACnn'nnGTG
Cuts at: 1166 2447 2492 1166
Size: 1281 45 4120
Fragments arranged by size:
4120 1281 45
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2546 1184
Size: 1362 4084
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2510 2542 2510
Size: 32 5414
AlwNI CAG_nnn'CTG
Cuts at: 3189 4761 3189
Size: 1572 3874
ApaLI G'TGCA_C
Cuts at: 3503 4659 3503
Size: 1156 4290
ApoI r'AATT_y
Cuts at: 1493 2456 2717 2755 2981 3133 5242 1493
Size: 963 261 38 226 152 2109 1697
Fragments arranged by size:
2109 1697 963 261 226 152 38
AseI AT'TA_AT
Cuts at: 160 1032 4000 160
Size: 872 2968 1606
Fragments arranged by size:
2968 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5446
AvaI C'yCGr_G
Cuts at: 1870 1943 2771 1870
Size: 73 828 4545
Fragments arranged by size:
4545 828 73
BamHI G'GATC_C
Cuts at: 2776 3143 2776
Size: 367 5079
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2734 2767 4222 618
Size: 325 217 627 947 33 1455 1842
Fragments arranged by size:
1842 1455 947 627 325 217 33
BanII G_rGCy'C
Cuts at: 1391 2426 2441 2765 1391
Size: 1035 15 324 4072
Fragments arranged by size:
4072 1035 324 15
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2031 928
Size: 971 132 4343
Fragments arranged by size:
4343 971 132
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3637 3671 1903
Size: 34 1700 34 3678
Fragments arranged by size:
3678 1700 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3338 4548 3338
Size: 1210 4236
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5446
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4057 136
Size: 107 122 71 631 605 2385 1525
Fragments arranged by size:
2385 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5441 5441
Size: 5446
BlpI GC'TnA_GC
Cuts at: 2863 2863
Size: 5446
Bme1580I G_kGCm'C
Cuts at: 1790 3507 4663 1790
Size: 1717 1156 2573
Fragments arranged by size:
2573 1717 1156
BmgBI CAC'GTC
Cuts at: 1318 2217 2376 2526 1318
Size: 899 159 150 4238
Fragments arranged by size:
4238 899 159 150
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3177 4135 442
Size: 416 53 83 399 24 1760 958 1753
Fragments arranged by size:
1760 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5446
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2460 2460
Size: 5446
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2078 2135 4106 1239
Size: 839 57 1971 2579
Fragments arranged by size:
2579 1971 839 57
Bpu10I CC'TnA_GC
Cuts at: 2435 2435
Size: 5446
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3571 4436 4734 3571
Size: 865 298 4283
Fragments arranged by size:
4283 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4109 882
Size: 617 2610 2219
Fragments arranged by size:
2610 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3237 493
Size: 711 2033 2702
Fragments arranged by size:
2702 2033 711
BsaBI GATnn'nnATC
Cuts at: 5440 5440
Size: 5446
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3253 3635 4270 275
Size: 53 83 186 936 1720 382 635 1451
Fragments arranged by size:
1720 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3879 4277 4551 4698 5109 3879
Size: 398 274 147 411 4216
Fragments arranged by size:
4216 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2363 2393 2573 2603 1178
Size: 30 165 30 960 30 180 30 4021
Fragments arranged by size:
4021 960 180 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5446
BseYI C'CCAG_C
Cuts at: 4649 5145 4649
Size: 496 4950
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2432 2506 1163
Size: 573 696 74 4103
Fragments arranged by size:
4103 696 573 74
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2896 3657 4685 1540
Size: 275 186 895 761 1028 2301
Fragments arranged by size:
2301 1028 895 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2426 2441 3507 3592 4663 2426
Size: 15 1066 85 1071 3209
Fragments arranged by size:
3209 1071 1066 85 15
BsmI GAATG_Cn'
Cuts at: 2957 3050 2957
Size: 93 5353
BsmBI CGTCTCn'nnnn_
Cuts at: 2207 2207
Size: 5446
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2426 2441 2765 3507 3592 4663 1391
Size: 399 636 15 324 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 636 399 324 85 15
BspEI T'CCGG_A
Cuts at: 5109 5109
Size: 5446
BspHI T'CATG_A
Cuts at: 2713 3335 5065 2713
Size: 622 1730 3094
Fragments arranged by size:
3094 1730 622
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2196 2801 844
Size: 1352 605 3489
Fragments arranged by size:
3489 1352 605
BsrBI CCG'CTC
Cuts at: 1394 1823 3333 1394
Size: 429 1510 3507
Fragments arranged by size:
3507 1510 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3941 4115 65
Size: 1183 2693 174 1396
Fragments arranged by size:
2693 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4090 4277 1700
Size: 110 2280 187 2869
Fragments arranged by size:
2869 2280 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5446
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5446
BssSI C'ACGA_G
Cuts at: 3506 4518 3506
Size: 1012 4434
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5446
BstBI TT'CG_AA
Cuts at: 4250 4250
Size: 5446
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5446
BstXI CCAn_nnnn'nTGG
Cuts at: 2113 2347 2389 2449 2113
Size: 234 42 60 5110
Fragments arranged by size:
5110 234 60 42
BstYI r'GATC_y
Cuts at: 1972 2776 3143 3528 3545 4997 5083 5095 5441
Size: 804 367 385 17 1452 86 12 346
Cuts at: 5441 1972
Size: 1977
Fragments arranged by size:
1977 1452 804 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2599 2599
Size: 5446
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3955
Fragments arranged by size:
3955 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4707
Fragments arranged by size:
4707 683 56
BtsI GCAGTG_nn'
Cuts at: 2028 2962 3756 3776 2028
Size: 934 794 20 3698
Fragments arranged by size:
3698 934 794 20
ClaI AT'CG_AT
Cuts at: 1554 2080 1554
Size: 526 4920
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5411
DraI TTT'AAA
Cuts at: 2750 2750
Size: 5446
DraIII CAC_nnn'GTG
Cuts at: 2183 2183
Size: 5446
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4453 809
Size: 507 3137 1802
Fragments arranged by size:
3137 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2893 3782
Size: 54 1425 50 275 18 177 886 889
Cuts at: 3782 5411 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 886 275 177 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2893 1537
Size: 275 1081 4090
Fragments arranged by size:
4090 1081 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3376 1353
Size: 2023 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4033 4407 4553 387
Size: 1064 138 2444 374 146 1280
Fragments arranged by size:
2444 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2424 2439 2424
Size: 15 5431
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2043 2078 2135 2569 3509 4106 4893
Size: 393 411 35 57 434 940 597 787
Cuts at: 4893 5161 1239
Size: 268 1524
Fragments arranged by size:
1524 940 787 597 434 411 393 268
57 35
EcoRI G'AATT_C
Cuts at: 2755 3133 2755
Size: 378 5068
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2651 2651
Size: 5446
FspI TGC'GCA
Cuts at: 3952 3952
Size: 5446
HaeII r_GCGC'y
Cuts at: 1678 4593 1678
Size: 2915 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4087 4119 4161 4193 1373
Size: 32 135 32 2515 32 42 32 2626
Fragments arranged by size:
2626 2515 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2015 2790 3036 669
Size: 988 281 77 775 246 3079
Fragments arranged by size:
3079 988 775 281 246 77
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5446
HpaI GTT'AAC
Cuts at: 3036 3036
Size: 5446
KpnI G_GTAC'C
Cuts at: 2771 2771
Size: 5446
MfeI C'AATT_G
Cuts at: 3045 3045
Size: 5446
MluI A'CGCG_T
Cuts at: 5322 5322
Size: 5446
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2406 4560 4744 1305
Size: 1101 2154 184 2007
Fragments arranged by size:
2154 2007 1101 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 10
Size: 54 1425 343 177 3447
Fragments arranged by size:
3447 1425 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2408 2447 2492 2552 3404 3763 3922
Size: 648 1242 39 45 60 852 359 159
Cuts at: 3922 518
Size: 2042
Fragments arranged by size:
2042 1242 852 648 359 159 60 45
39
MspA1I CmG'CkG
Cuts at: 1606 2862 3539 4687 4932 1606
Size: 1256 677 1148 245 2120
Fragments arranged by size:
2120 1256 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3955
Fragments arranged by size:
3955 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5446
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5446
NotI GC'GGCC_GC
Cuts at: 2893 2893
Size: 5446
NspI r_CATG'y
Cuts at: 1132 1229 2804 4349 1132
Size: 97 1575 1545 2229
Fragments arranged by size:
2229 1575 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5446
PciI A'CATG_T
Cuts at: 4345 4345
Size: 5446
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5446
PmeI GTTT'AAAC
Cuts at: 2750 2750
Size: 5446
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3515 3547 5028 5060 3515
Size: 32 1481 32 3901
Fragments arranged by size:
3901 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5446
PsiI TTA'TAA
Cuts at: 3016 3016
Size: 5446
PstI C_TGCA'G
Cuts at: 830 2207 2798 830
Size: 1377 591 3478
Fragments arranged by size:
3478 1377 591
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5446
SacI G_AGCT'C
Cuts at: 2426 2441 2426
Size: 15 5431
SalI G'TCGA_C
Cuts at: 1936 2788 1936
Size: 852 4594
SbfI CC_TGCA'GG
Cuts at: 2798 2798
Size: 5446
ScaI AGT'ACT
Cuts at: 3694 5406 3694
Size: 1712 3734
SfcI C'TryA_G
Cuts at: 826 1045 2203 2794 3929 4610 4801 826
Size: 219 1158 591 1135 681 191 1471
Fragments arranged by size:
1471 1158 1135 681 591 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5446
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5446
SmaI CCC'GGG
Cuts at: 1872 2773 1872
Size: 901 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3550 4451 4713 820
Size: 197 926 1607 901 262 1553
Fragments arranged by size:
1607 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3237 493
Size: 2744 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5446
SphI G_CATG'C
Cuts at: 1132 2804 1132
Size: 1672 3774
SspI AAT'ATT
Cuts at: 5 52 3370 5
Size: 47 3318 2081
Fragments arranged by size:
3318 2081 47
StuI AGG'CCT
Cuts at: 1517 2045 2467 1517
Size: 528 422 4496
Fragments arranged by size:
4496 528 422
StyI C'CwwG_G
Cuts at: 513 1065 2004 2635 2885 513
Size: 552 939 631 250 3074
Fragments arranged by size:
3074 939 631 552 250
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3473 3490 3643 3828 4273 325
Size: 1255 1893 17 153 185 445 1498
Fragments arranged by size:
1893 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3692 5404 96
Size: 274 80 33 51 1443 1715 1712 138
Fragments arranged by size:
1715 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2363 2395 5406 5438 2363
Size: 32 3011 32 2371
Fragments arranged by size:
3011 2371 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2483 1316
Size: 1167 4279
XbaI T'CTAG_A
Cuts at: 2722 2782 2722
Size: 60 5386
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5446
XmaI C'CCGG_G
Cuts at: 1870 2771 1870
Size: 901 4545
XmnI GAAnn'nnTTC
Cuts at: 3575 3575
Size: 5446
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3254 276
Size: 53 83 186 936 1720 2468
Fragments arranged by size:
2468 1720 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BstZ17I EcoNI EcoO109I EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI