Restriction map for
pFN21AB8772
(Circular) (Six-base) MAPSORT of: pFN21AB8772.seq Check: 1572 from: 1 to: 6379
pFN21AB8772 6379 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4189 278
Size: 53 83 186 936 2653 2468
Fragments arranged by size:
2653 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3722 1937
Size: 1785 4594
Acc65I G'GTAC_C
Cuts at: 3700 3700
Size: 6379
AclI AA'CG_TT
Cuts at: 1563 4506 4879 1563
Size: 2943 373 3063
Fragments arranged by size:
3063 2943 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2391 2589 4442 5826 6094 1632
Size: 759 198 1853 1384 268 1917
Fragments arranged by size:
1917 1853 1384 759 268 198
AflII C'TTAA_G
Cuts at: 820 1017 2960 820
Size: 197 1943 4239
Fragments arranged by size:
4239 1943 197
AflIII A'CryG_T
Cuts at: 1203 5278 6255 1203
Size: 4075 977 1327
Fragments arranged by size:
4075 1327 977
AgeI A'CCGG_T
Cuts at: 5210 5210
Size: 6379
AhdI GACnn_n'nnGTC
Cuts at: 5108 5108
Size: 6379
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6379
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2327 1184
Size: 1143 5236
AlwNI CAG_nnn'CTG
Cuts at: 2123 4122 5694 2123
Size: 1999 1572 2808
Fragments arranged by size:
2808 1999 1572
ApaLI G'TGCA_C
Cuts at: 4436 5592 4436
Size: 1156 5223
ApoI r'AATT_y
Cuts at: 1493 3272 3508 3688 3914 4066 6175 1493
Size: 1779 236 180 226 152 2109 1697
Fragments arranged by size:
2109 1779 1697 236 226 180 152
AseI AT'TA_AT
Cuts at: 160 1032 3665 4933 160
Size: 872 2633 1268 1606
Fragments arranged by size:
2633 1606 1268 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6379
AvaI C'yCGr_G
Cuts at: 1870 1943 3704 1870
Size: 73 1761 4545
Fragments arranged by size:
4545 1761 73
BamHI G'GATC_C
Cuts at: 2974 3709 4076 2974
Size: 735 367 5277
Fragments arranged by size:
5277 735 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3700 5155 618
Size: 325 217 627 1913 1455 1842
Fragments arranged by size:
1913 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3698 1391
Size: 2307 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3141 928
Size: 971 1242 4166
Fragments arranged by size:
4166 1242 971
BbvCI CC'TCA_GC
Cuts at: 2046 2124 2046
Size: 78 6301
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4570 4604 1903
Size: 34 2633 34 3678
Fragments arranged by size:
3678 2633 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4271 5481 4271
Size: 1210 5169
BclI T'GATC_A
Cuts at: 1548 2235 3411 1548
Size: 687 1176 4516
Fragments arranged by size:
4516 1176 687
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4990 136
Size: 107 122 71 631 605 3318 1525
Fragments arranged by size:
3318 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6374 6374
Size: 6379
BlpI GC'TnA_GC
Cuts at: 3796 3796
Size: 6379
Bme1580I G_kGCm'C
Cuts at: 1790 3261 4440 5596 1790
Size: 1471 1179 1156 2573
Fragments arranged by size:
2573 1471 1179 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6379
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2451 4110 5068
Size: 416 53 83 399 24 1034 1659 958
Cuts at: 5068 442
Size: 1753
Fragments arranged by size:
1753 1659 1034 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6379
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2553 2718 3135 5039 1239
Size: 1314 165 417 1904 2579
Fragments arranged by size:
2579 1904 1314 417 165
Bpu10I CC'TnA_GC
Cuts at: 2046 2124 2046
Size: 78 6301
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3021 3283 4504 5369 5667 3021
Size: 262 1221 865 298 3733
Fragments arranged by size:
3733 1221 865 298 262
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2462 2570 2753 5042 882
Size: 617 963 108 183 2289 2219
Fragments arranged by size:
2289 2219 963 617 183 108
BsaAI yAC'GTr
Cuts at: 493 1204 2912 4170 493
Size: 711 1708 1258 2702
Fragments arranged by size:
2702 1708 1258 711
BsaBI GATnn'nnATC
Cuts at: 6373 6373
Size: 6379
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4186 4568 5203 275
Size: 53 83 186 936 2653 382 635 1451
Fragments arranged by size:
2653 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4812 5210 5484 5631 6042 4812
Size: 398 274 147 411 5149
Fragments arranged by size:
5149 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6154
Fragments arranged by size:
6154 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2766 1186
Size: 1580 4799
BseYI C'CCAG_C
Cuts at: 5582 6078 5582
Size: 496 5883
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3251 1163
Size: 573 1515 4291
Fragments arranged by size:
4291 1515 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3829 4590 5618 1540
Size: 275 186 1828 761 1028 2301
Fragments arranged by size:
2301 1828 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3630 4440 4525 5596 3630
Size: 810 85 1071 4413
Fragments arranged by size:
4413 1071 810 85
BsmI GAATG_Cn'
Cuts at: 3890 3983 3890
Size: 93 6286
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3261 3630 3698 4440 4525 5596 1391
Size: 399 1471 369 68 742 85 1071 2174
Fragments arranged by size:
2174 1471 1071 742 399 369 85 68
BspEI T'CCGG_A
Cuts at: 6042 6042
Size: 6379
BspHI T'CATG_A
Cuts at: 2986 3049 4268 5998 2986
Size: 63 1219 1730 3367
Fragments arranged by size:
3367 1730 1219 63
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3734 844
Size: 2890 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4266 1394
Size: 429 2443 3507
Fragments arranged by size:
3507 2443 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2184 4874 5048 65
Size: 1183 936 2690 174 1396
Fragments arranged by size:
2690 1396 1183 936 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5023 5210 1700
Size: 110 3213 187 2869
Fragments arranged by size:
3213 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6379
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6379
BssSI C'ACGA_G
Cuts at: 4439 5451 4439
Size: 1012 5367
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2728 794
Size: 1934 4445
BstBI TT'CG_AA
Cuts at: 5183 5183
Size: 6379
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6379
BstYI r'GATC_y
Cuts at: 1972 2610 2802 2974 3709 4076 4461 4478 5930
Size: 638 192 172 735 367 385 17 1452
Cuts at: 5930 6016 6028 6374 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 735 638 385 367 346 192
172 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4888
Fragments arranged by size:
4888 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5640
Fragments arranged by size:
5640 683 56
BtsI GCAGTG_nn'
Cuts at: 2082 2124 2899 3895 4689 4709 2082
Size: 42 775 996 794 20 3752
Fragments arranged by size:
3752 996 794 775 42 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6379
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2893 2928 529
Size: 35 2329 35 3980
Fragments arranged by size:
3980 2329 35 35
DraI TTT'AAA
Cuts at: 3363 3683 3363
Size: 320 6059
DraIII CAC_nnn'GTG
Cuts at: 3289 3289
Size: 6379
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5386 809
Size: 507 4070 1802
Fragments arranged by size:
4070 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3826 4715 6344
Size: 54 1425 50 275 18 1996 889 1629
Cuts at: 6344 8
Size: 43
Fragments arranged by size:
1996 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3826 1537
Size: 275 2014 4090
Fragments arranged by size:
4090 2014 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2460 2532 2568 2589 4309 1353
Size: 1107 72 36 21 1720 3423
Fragments arranged by size:
3423 1720 1107 72 36 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4966 5340 5486 387
Size: 1064 138 3377 374 146 1280
Fragments arranged by size:
3377 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2391 2553 2589 2718 3135 4442 5039
Size: 393 759 162 36 129 417 1307 597
Cuts at: 5039 5826 6094 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1307 787 759 597 417 393 268
162 129 36
EcoNI CCTnn'n_nnAGG
Cuts at: 2547 2736 2547
Size: 189 6190
EcoO109I rG'GnC_Cy
Cuts at: 2409 2553 2409
Size: 144 6235
EcoRI G'AATT_C
Cuts at: 3688 4066 3688
Size: 378 6001
FspI TGC'GCA
Cuts at: 4885 4885
Size: 6379
HaeII r_GCGC'y
Cuts at: 1678 5526 1678
Size: 3848 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2758 2790 3125 3157 3396
Size: 32 135 32 1186 32 335 32 239
Cuts at: 3396 3428 3639 3671 5020 5052 5094 5126 1373
Size: 32 211 32 1349 32 42 32 2626
Fragments arranged by size:
2626 1349 1186 335 239 211 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2011 2967 3723 3969 669
Size: 988 281 73 956 756 246 3079
Fragments arranged by size:
3079 988 956 756 281 246 73
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6379
HpaI GTT'AAC
Cuts at: 2967 3969 2967
Size: 1002 5377
KpnI G_GTAC'C
Cuts at: 3704 3704
Size: 6379
MfeI C'AATT_G
Cuts at: 3978 3978
Size: 6379
MluI A'CGCG_T
Cuts at: 6255 6255
Size: 6379
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5493 5677 1305
Size: 4188 184 2007
Fragments arranged by size:
4188 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4557
Fragments arranged by size:
4557 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2339 2480 4337 4696 4855 518
Size: 648 1173 141 1857 359 159 2042
Fragments arranged by size:
2042 1857 1173 648 359 159 141
MspA1I CmG'CkG
Cuts at: 1606 3235 3795 4472 5620 5865 1606
Size: 1629 560 677 1148 245 2120
Fragments arranged by size:
2120 1629 1148 677 560 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4888
Fragments arranged by size:
4888 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6379
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6379
NotI GC'GGCC_GC
Cuts at: 3826 3826
Size: 6379
NspI r_CATG'y
Cuts at: 1132 1229 3737 5282 1132
Size: 97 2508 1545 2229
Fragments arranged by size:
2508 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6379
PciI A'CATG_T
Cuts at: 5278 5278
Size: 6379
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6379
PfoI T'CCnGG_A
Cuts at: 2613 3582 2613
Size: 969 5410
PmeI GTTT'AAAC
Cuts at: 3683 3683
Size: 6379
PmlI CAC'GTG
Cuts at: 2912 2912
Size: 6379
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4448 4480 5961 5993 4448
Size: 32 1481 32 4834
Fragments arranged by size:
4834 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2409 2553 2409
Size: 144 6235
PshAI GACnn'nnGTC
Cuts at: 1613 2264 1613
Size: 651 5728
PsiI TTA'TAA
Cuts at: 2595 3949 2595
Size: 1354 5025
PstI C_TGCA'G
Cuts at: 830 3731 830
Size: 2901 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6379
PvuII CAG'CTG
Cuts at: 3235 3235
Size: 6379
SalI G'TCGA_C
Cuts at: 1936 3721 1936
Size: 1785 4594
SapI GCTCTTCn'nnn_
Cuts at: 2532 2532
Size: 6379
SbfI CC_TGCA'GG
Cuts at: 3731 3731
Size: 6379
ScaI AGT'ACT
Cuts at: 2356 3426 4627 6339 2356
Size: 1070 1201 1712 2396
Fragments arranged by size:
2396 1712 1201 1070
SfcI C'TryA_G
Cuts at: 826 1045 2203 3727 4862 5543 5734 826
Size: 219 1158 1524 1135 681 191 1471
Fragments arranged by size:
1524 1471 1158 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6379
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6379
SmaI CCC'GGG
Cuts at: 1872 3706 1872
Size: 1834 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2960 3036 3262 4483 5384 5646
Size: 197 926 1017 76 226 1221 901 262
Cuts at: 5646 820
Size: 1553
Fragments arranged by size:
1553 1221 1017 926 901 262 226 197
76
SnaBI TAC'GTA
Cuts at: 493 4170 493
Size: 3677 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6379
SphI G_CATG'C
Cuts at: 1132 3737 1132
Size: 2605 3774
SspI AAT'ATT
Cuts at: 5 52 2404 3475 3484 3495 3579 4303 5
Size: 47 2352 1071 9 11 84 724 2081
Fragments arranged by size:
2352 2081 1071 724 84 47 11 9
StuI AGG'CCT
Cuts at: 1517 2894 1517
Size: 1377 5002
StyI C'CwwG_G
Cuts at: 513 1065 2004 3543 3818 513
Size: 552 939 1539 275 3074
Fragments arranged by size:
3074 1539 939 552 275
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4406 4423 4576 4761 5206 325
Size: 1255 2826 17 153 185 445 1498
Fragments arranged by size:
2826 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2354 3424 4625
Size: 274 80 33 51 1443 377 1070 1201
Cuts at: 4625 6337 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1201 1070 377 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6339 6371 6339
Size: 32 6347
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6379
XbaI T'CTAG_A
Cuts at: 3715 3715
Size: 6379
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2162 2742 2162
Size: 580 5799
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6379
XmaI C'CCGG_G
Cuts at: 1870 3704 1870
Size: 1834 4545
XmnI GAAnn'nnTTC
Cuts at: 4508 4508
Size: 6379
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4187 276
Size: 53 83 186 936 2653 2468
Fragments arranged by size:
2653 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SexAI SfiI SfoI SrfI SwaI