Restriction map for
pFN21AB8764
(Circular) (Six-base) MAPSORT of: pFN21AB8764.seq Check: 649 from: 1 to: 5359
pFN21AB8764 5359 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2243 2245 2428 2243
Size: 2 183 5174
Fragments arranged by size:
5174 183 2
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3169 278
Size: 53 83 186 936 1633 2468
Fragments arranged by size:
2468 1633 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2702 1937
Size: 765 4594
Acc65I G'GTAC_C
Cuts at: 2680 2680
Size: 5359
AclI AA'CG_TT
Cuts at: 1563 3486 3859 1563
Size: 1923 373 3063
Fragments arranged by size:
3063 1923 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3422 4806 5074 1632
Size: 1790 1384 268 1917
Fragments arranged by size:
1917 1790 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5162
AflIII A'CryG_T
Cuts at: 1203 4258 5235 1203
Size: 3055 977 1327
Fragments arranged by size:
3055 1327 977
AgeI A'CCGG_T
Cuts at: 4190 4190
Size: 5359
AhdI GACnn_n'nnGTC
Cuts at: 4088 4088
Size: 5359
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5359
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5359
AlwNI CAG_nnn'CTG
Cuts at: 3102 4674 3102
Size: 1572 3787
ApaI G_GGCC'C
Cuts at: 2391 2391
Size: 5359
ApaLI G'TGCA_C
Cuts at: 3416 4572 3416
Size: 1156 4203
ApoI r'AATT_y
Cuts at: 1493 2668 2894 3046 5155 1493
Size: 1175 226 152 2109 1697
Fragments arranged by size:
2109 1697 1175 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3913 160
Size: 872 2881 1606
Fragments arranged by size:
2881 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5359
AvaI C'yCGr_G
Cuts at: 1870 1943 2684 1870
Size: 73 741 4545
Fragments arranged by size:
4545 741 73
BamHI G'GATC_C
Cuts at: 2371 2689 3056 2371
Size: 318 367 4674
Fragments arranged by size:
4674 367 318
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2156 2211 2285 2444 2680
Size: 325 217 627 369 55 74 159 236
Cuts at: 2680 4135 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 369 325 236 217 159
74 55
BanII G_rGCy'C
Cuts at: 1391 2391 2545 2678 1391
Size: 1000 154 133 4072
Fragments arranged by size:
4072 1000 154 133
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2025 928
Size: 971 126 4262
Fragments arranged by size:
4262 971 126
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3550 3584 1903
Size: 34 1613 34 3678
Fragments arranged by size:
3678 1613 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3251 4461 3251
Size: 1210 4149
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5359
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2247 2386 3970
Size: 107 122 71 631 605 575 139 1584
Cuts at: 3970 136
Size: 1525
Fragments arranged by size:
1584 1525 631 605 575 139 122 107
71
BglII A'GATC_T
Cuts at: 5354 5354
Size: 5359
BlpI GC'TnA_GC
Cuts at: 2776 2776
Size: 5359
Bme1580I G_kGCm'C
Cuts at: 1790 2159 2214 2288 2391 2447 3420 4576 1790
Size: 369 55 74 103 56 973 1156 2573
Fragments arranged by size:
2573 1156 973 369 103 74 56 55
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5359
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2428 3090 4048
Size: 416 53 83 399 24 1011 662 958
Cuts at: 4048 442
Size: 1753
Fragments arranged by size:
1753 1011 958 662 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5359
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2354 2453 2567 2591 4019 1239
Size: 1115 99 114 24 1428 2579
Fragments arranged by size:
2579 1428 1115 114 99 24
Bpu10I CC'TnA_GC
Cuts at: 2486 2486
Size: 5359
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3484 4349 4647 3484
Size: 865 298 4196
Fragments arranged by size:
4196 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4022 882
Size: 617 2523 2219
Fragments arranged by size:
2523 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3150 493
Size: 711 1946 2702
Fragments arranged by size:
2702 1946 711
BsaBI GATnn'nnATC
Cuts at: 5353 5353
Size: 5359
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3166 3548 4183 275
Size: 53 83 186 936 1633 382 635 1451
Fragments arranged by size:
1633 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3792 4190 4464 4611 5022 3792
Size: 398 274 147 411 4129
Fragments arranged by size:
4129 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2091 2121 2564 2594 1178
Size: 30 165 30 688 30 443 30 3943
Fragments arranged by size:
3943 688 443 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2045 2090 2342 2564 1186
Size: 859 45 252 222 3981
Fragments arranged by size:
3981 859 252 222 45
BseYI C'CCAG_C
Cuts at: 2207 2357 2391 4562 5058 2207
Size: 150 34 2171 496 2508
Fragments arranged by size:
2508 2171 496 150 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2277 1163
Size: 573 541 4245
Fragments arranged by size:
4245 573 541
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2809 3570 4598 1540
Size: 275 186 808 761 1028 2301
Fragments arranged by size:
2301 1028 808 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3420 3505 4576 3420
Size: 85 1071 4203
Fragments arranged by size:
4203 1071 85
BsmI GAATG_Cn'
Cuts at: 2870 2963 2870
Size: 93 5266
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2159 2214 2288 2391 2447 2545 2678
Size: 399 369 55 74 103 56 98 133
Cuts at: 2678 3420 3505 4576 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 369 133 103 98
85 74 56 55
BspEI T'CCGG_A
Cuts at: 5022 5022
Size: 5359
BspHI T'CATG_A
Cuts at: 3248 4978 3248
Size: 1730 3629
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2243 2245 2428 2714 844
Size: 1399 2 183 286 3489
Fragments arranged by size:
3489 1399 286 183 2
BsrBI CCG'CTC
Cuts at: 1394 1823 3246 1394
Size: 429 1423 3507
Fragments arranged by size:
3507 1423 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3854 4028 65
Size: 1183 2606 174 1396
Fragments arranged by size:
2606 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4003 4190 1700
Size: 110 2193 187 2869
Fragments arranged by size:
2869 2193 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5359
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5359
BssSI C'ACGA_G
Cuts at: 2202 3419 4431 2202
Size: 1217 1012 3130
Fragments arranged by size:
3130 1217 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5359
BstBI TT'CG_AA
Cuts at: 4163 4163
Size: 5359
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5359
BstYI r'GATC_y
Cuts at: 1972 2371 2689 3056 3441 3458 4910 4996 5008
Size: 399 318 367 385 17 1452 86 12
Cuts at: 5008 5354 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 399 385 367 346 318 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2263 2347 2383 2448 513
Size: 552 1198 84 36 65 3424
Fragments arranged by size:
3424 1198 552 84 65 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4620
Fragments arranged by size:
4620 683 56
BtsI GCAGTG_nn'
Cuts at: 2289 2508 2875 3669 3689 2289
Size: 219 367 794 20 3959
Fragments arranged by size:
3959 794 367 219 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5359
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2389 2424 529
Size: 35 1825 35 3464
Fragments arranged by size:
3464 1825 35 35
DraI TTT'AAA
Cuts at: 2663 2663
Size: 5359
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4366 809
Size: 507 3050 1802
Fragments arranged by size:
3050 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2344 2806 3695
Size: 54 1425 50 275 18 514 462 889
Cuts at: 3695 5324 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 514 462 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2806 1537
Size: 275 994 4090
Fragments arranged by size:
4090 994 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2022 2076 2333 3289 1353
Size: 669 54 257 956 3423
Fragments arranged by size:
3423 956 669 257 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3946 4320 4466 387
Size: 1064 138 2357 374 146 1280
Fragments arranged by size:
2357 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2354 2453 2567 2591 3422 4019 4806
Size: 393 722 99 114 24 831 597 787
Cuts at: 4806 5074 1239
Size: 268 1524
Fragments arranged by size:
1524 831 787 722 597 393 268 114
99 24
EcoNI CCTnn'n_nnAGG
Cuts at: 2435 2549 2435
Size: 114 5245
EcoO109I rG'GnC_Cy
Cuts at: 2379 2388 2379
Size: 9 5350
EcoRI G'AATT_C
Cuts at: 2668 3046 2668
Size: 378 4981
FspI TGC'GCA
Cuts at: 3865 3865
Size: 5359
HaeII r_GCGC'y
Cuts at: 1678 2368 4506 1678
Size: 690 2138 2531
Fragments arranged by size:
2531 2138 690
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4000 4032 4074 4106 1373
Size: 32 135 32 2428 32 42 32 2626
Fragments arranged by size:
2626 2428 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2278 2703 2949 669
Size: 988 281 340 425 246 3079
Fragments arranged by size:
3079 988 425 340 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5359
HpaI GTT'AAC
Cuts at: 2949 2949
Size: 5359
KpnI G_GTAC'C
Cuts at: 2684 2684
Size: 5359
MfeI C'AATT_G
Cuts at: 2958 2958
Size: 5359
MluI A'CGCG_T
Cuts at: 5235 5235
Size: 5359
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4473 4657 1305
Size: 3168 184 2007
Fragments arranged by size:
3168 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2346 10
Size: 54 1425 343 514 3023
Fragments arranged by size:
3023 1425 514 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2138 3317 3676 3835 518
Size: 648 972 1179 359 159 2042
Fragments arranged by size:
2042 1179 972 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2614 2775 3452 4600 4845 1606
Size: 1008 161 677 1148 245 2120
Fragments arranged by size:
2120 1148 1008 677 245 161
NcoI C'CATG_G
Cuts at: 513 1065 2347 2448 513
Size: 552 1282 101 3424
Fragments arranged by size:
3424 1282 552 101
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5359
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5359
NotI GC'GGCC_GC
Cuts at: 2806 2806
Size: 5359
NspI r_CATG'y
Cuts at: 1132 1229 2320 2610 2717 4262 1132
Size: 97 1091 290 107 1545 2229
Fragments arranged by size:
2229 1545 1091 290 107 97
PasI CC'CwG_GG
Cuts at: 1348 2270 1348
Size: 922 4437
PciI A'CATG_T
Cuts at: 4258 4258
Size: 5359
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5359
PfoI T'CCnGG_A
Cuts at: 2430 2568 2430
Size: 138 5221
PmeI GTTT'AAAC
Cuts at: 2663 2663
Size: 5359
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3428 3460 4941 4973 3428
Size: 32 1481 32 3814
Fragments arranged by size:
3814 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5359
PsiI TTA'TAA
Cuts at: 2929 2929
Size: 5359
PspOMI G'GGCC_C
Cuts at: 2387 2387
Size: 5359
PstI C_TGCA'G
Cuts at: 830 2635 2711 830
Size: 1805 76 3478
Fragments arranged by size:
3478 1805 76
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5359
SalI G'TCGA_C
Cuts at: 1936 2701 1936
Size: 765 4594
SbfI CC_TGCA'GG
Cuts at: 2711 2711
Size: 5359
ScaI AGT'ACT
Cuts at: 3607 5319 3607
Size: 1712 3647
SfcI C'TryA_G
Cuts at: 826 1045 2631 2707 3842 4523 4714 826
Size: 219 1586 76 1135 681 191 1471
Fragments arranged by size:
1586 1471 1135 681 219 191 76
SfiI GGCCn_nnn'nGGCC
Cuts at: 2386 2386
Size: 5359
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5359
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5359
SmaI CCC'GGG
Cuts at: 1872 2686 1872
Size: 814 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3463 4364 4626 820
Size: 197 926 1520 901 262 1553
Fragments arranged by size:
1553 1520 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3150 493
Size: 2657 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5359
SphI G_CATG'C
Cuts at: 1132 2320 2610 2717 1132
Size: 1188 290 107 3774
Fragments arranged by size:
3774 1188 290 107
SspI AAT'ATT
Cuts at: 5 52 2183 3283 5
Size: 47 2131 1100 2081
Fragments arranged by size:
2131 2081 1100 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5359
StyI C'CwwG_G
Cuts at: 513 1065 2347 2375 2448 2798 513
Size: 552 1282 28 73 350 3074
Fragments arranged by size:
3074 1282 552 350 73 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3386 3403 3556 3741 4186 325
Size: 1255 1806 17 153 185 445 1498
Fragments arranged by size:
1806 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2638 3605 5317
Size: 274 80 33 51 1443 661 967 1712
Cuts at: 5317 96
Size: 138
Fragments arranged by size:
1712 1443 967 661 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2564 2596 5319 5351 2564
Size: 32 2723 32 2572
Fragments arranged by size:
2723 2572 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5359
XbaI T'CTAG_A
Cuts at: 2695 2695
Size: 5359
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2354 2354
Size: 5359
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5359
XmaI C'CCGG_G
Cuts at: 1870 2684 1870
Size: 814 4545
XmnI GAAnn'nnTTC
Cuts at: 3488 3488
Size: 5359
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3167 276
Size: 53 83 186 936 1633 2468
Fragments arranged by size:
2468 1633 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI SalI SbfI ScaI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfoI SrfI
SwaI