Restriction map for
pFN21AB8760
(Circular) (Six-base) MAPSORT of: pFN21AB8760.seq Check: 3747 from: 1 to: 5314
pFN21AB8760 5314 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2111 2111
Size: 5314
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3124 278
Size: 53 83 186 936 1588 2468
Fragments arranged by size:
2468 1588 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2657 1937
Size: 720 4594
Acc65I G'GTAC_C
Cuts at: 2635 2635
Size: 5314
AclI AA'CG_TT
Cuts at: 1563 3441 3814 1563
Size: 1878 373 3063
Fragments arranged by size:
3063 1878 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3377 4761 5029 1632
Size: 1745 1384 268 1917
Fragments arranged by size:
1917 1745 1384 268
AfeI AGC'GCT
Cuts at: 2591 2591
Size: 5314
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5117
AflIII A'CryG_T
Cuts at: 1203 2571 4213 5190 1203
Size: 1368 1642 977 1327
Fragments arranged by size:
1642 1368 1327 977
AgeI A'CCGG_T
Cuts at: 4145 4145
Size: 5314
AhdI GACnn_n'nnGTC
Cuts at: 4043 4043
Size: 5314
AleI CACnn'nnGTG
Cuts at: 1166 2399 1166
Size: 1233 4081
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5314
AlwNI CAG_nnn'CTG
Cuts at: 2428 3057 4629 2428
Size: 629 1572 3113
Fragments arranged by size:
3113 1572 629
ApaLI G'TGCA_C
Cuts at: 2512 3371 4527 2512
Size: 859 1156 3299
Fragments arranged by size:
3299 1156 859
ApoI r'AATT_y
Cuts at: 1493 2623 2849 3001 5110 1493
Size: 1130 226 152 2109 1697
Fragments arranged by size:
2109 1697 1130 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3868 160
Size: 872 2836 1606
Fragments arranged by size:
2836 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5314
AvaI C'yCGr_G
Cuts at: 1870 1943 2372 2639 1870
Size: 73 429 267 4545
Fragments arranged by size:
4545 429 267 73
BamHI G'GATC_C
Cuts at: 2644 3011 2644
Size: 367 4947
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2154 2163 2635 4090 618
Size: 325 217 627 367 9 472 1455 1842
Fragments arranged by size:
1842 1455 627 472 367 325 217 9
BanII G_rGCy'C
Cuts at: 1391 2443 2633 1391
Size: 1052 190 4072
Fragments arranged by size:
4072 1052 190
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2266 2536 928
Size: 971 367 270 3706
Fragments arranged by size:
3706 971 367 270
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3505 3539 1903
Size: 34 1568 34 3678
Fragments arranged by size:
3678 1568 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3206 4416 3206
Size: 1210 4104
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5314
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3925 136
Size: 107 122 71 631 605 2253 1525
Fragments arranged by size:
2253 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2043 5309 2043
Size: 3266 2048
BlpI GC'TnA_GC
Cuts at: 2731 2731
Size: 5314
Bme1580I G_kGCm'C
Cuts at: 1790 2159 2516 3375 4531 1790
Size: 369 357 859 1156 2573
Fragments arranged by size:
2573 1156 859 369 357
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5314
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3045 4003 442
Size: 416 53 83 399 24 1628 958 1753
Fragments arranged by size:
1753 1628 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5314
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3974 1239
Size: 2735 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3439 4304 4602 3439
Size: 865 298 4151
Fragments arranged by size:
4151 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3977 882
Size: 617 2478 2219
Fragments arranged by size:
2478 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3105 493
Size: 711 1901 2702
Fragments arranged by size:
2702 1901 711
BsaBI GATnn'nnATC
Cuts at: 5308 5308
Size: 5314
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3121 3503 4138 275
Size: 53 83 186 936 1588 382 635 1451
Fragments arranged by size:
1588 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3747 4145 4419 4566 4977 3747
Size: 398 274 147 411 4084
Fragments arranged by size:
4084 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2000 2030 1178
Size: 30 165 30 597 30 4462
Fragments arranged by size:
4462 597 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2474 2477 2540 1186
Size: 1288 3 63 3960
Fragments arranged by size:
3960 1288 63 3
BseYI C'CCAG_C
Cuts at: 2288 4517 5013 2288
Size: 2229 496 2589
Fragments arranged by size:
2589 2229 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4741
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2764 3525 4553 1540
Size: 275 186 763 761 1028 2301
Fragments arranged by size:
2301 1028 763 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2516 3375 3460 4531 2516
Size: 859 85 1071 3299
Fragments arranged by size:
3299 1071 859 85
BsmI GAATG_Cn'
Cuts at: 2825 2918 2825
Size: 93 5221
BsmBI CGTCTCn'nnnn_
Cuts at: 2003 2003
Size: 5314
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2159 2443 2516 2633 3375 3460 4531
Size: 399 369 284 73 117 742 85 1071
Cuts at: 4531 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 369 284 117 85
73
BspEI T'CCGG_A
Cuts at: 4977 4977
Size: 5314
BspHI T'CATG_A
Cuts at: 3203 4933 3203
Size: 1730 3584
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2111 2669 844
Size: 1267 558 3489
Fragments arranged by size:
3489 1267 558
BsrBI CCG'CTC
Cuts at: 1394 1823 3201 1394
Size: 429 1378 3507
Fragments arranged by size:
3507 1378 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2299 3809 3983 65
Size: 1183 1051 1510 174 1396
Fragments arranged by size:
1510 1396 1183 1051 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3958 4145 1700
Size: 110 2148 187 2869
Fragments arranged by size:
2869 2148 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5314
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5314
BssSI C'ACGA_G
Cuts at: 3374 4386 3374
Size: 1012 4302
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2298 2428 794
Size: 1504 130 3680
Fragments arranged by size:
3680 1504 130
BstBI TT'CG_AA
Cuts at: 4118 4118
Size: 5314
BstEII G'GTnAC_C
Cuts at: 1184 2401 1184
Size: 1217 4097
BstXI CCAn_nnnn'nTGG
Cuts at: 2086 2086
Size: 5314
BstYI r'GATC_y
Cuts at: 1972 2043 2644 3011 3396 3413 4865 4951 4963
Size: 71 601 367 385 17 1452 86 12
Cuts at: 4963 5309 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 601 385 367 346 86 71
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2167 2397 513
Size: 552 939 163 230 3430
Fragments arranged by size:
3430 939 552 230 163
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2224 487
Size: 683 56 998 3577
Fragments arranged by size:
3577 998 683 56
BtsI GCAGTG_nn'
Cuts at: 2830 3624 3644 2830
Size: 794 20 4500
Fragments arranged by size:
4500 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5314
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5279
DraI TTT'AAA
Cuts at: 2618 2618
Size: 5314
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2225 4321 809
Size: 507 909 2096 1802
Fragments arranged by size:
2096 1802 909 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2761 3650 5279
Size: 54 1425 50 275 18 931 889 1629
Cuts at: 5279 8
Size: 43
Fragments arranged by size:
1629 1425 931 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2761 1537
Size: 275 949 4090
Fragments arranged by size:
4090 949 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3244 1353
Size: 1891 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3901 4275 4421 387
Size: 1064 138 2312 374 146 1280
Fragments arranged by size:
2312 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3377 3974 4761 5029 1239
Size: 393 1745 597 787 268 1524
Fragments arranged by size:
1745 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2430 2430
Size: 5314
EcoO109I rG'GnC_Cy
Cuts at: 2227 2227
Size: 5314
EcoRI G'AATT_C
Cuts at: 2623 3001 2623
Size: 378 4936
EcoRV GAT'ATC
Cuts at: 2604 2604
Size: 5314
FspI TGC'GCA
Cuts at: 3820 3820
Size: 5314
HaeII r_GCGC'y
Cuts at: 1678 2593 4461 1678
Size: 915 1868 2531
Fragments arranged by size:
2531 1868 915
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2000 2032 3955 3987 4029
Size: 32 135 32 428 32 1923 32 42
Cuts at: 4029 4061 1373
Size: 32 2626
Fragments arranged by size:
2626 1923 428 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2658 2904 669
Size: 988 281 720 246 3079
Fragments arranged by size:
3079 988 720 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5314
HpaI GTT'AAC
Cuts at: 2904 2904
Size: 5314
KpnI G_GTAC'C
Cuts at: 2639 2639
Size: 5314
MfeI C'AATT_G
Cuts at: 2913 2913
Size: 5314
MluI A'CGCG_T
Cuts at: 5190 5190
Size: 5314
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4428 4612 1305
Size: 3123 184 2007
Fragments arranged by size:
3123 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3492
Fragments arranged by size:
3492 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2399 2525 3272 3631 3790 518
Size: 648 1233 126 747 359 159 2042
Fragments arranged by size:
2042 1233 747 648 359 159 126
MspA1I CmG'CkG
Cuts at: 1606 2169 2730 3407 4555 4800 1606
Size: 563 561 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 563 561 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3823
Fragments arranged by size:
3823 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5314
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5314
NotI GC'GGCC_GC
Cuts at: 2761 2761
Size: 5314
NspI r_CATG'y
Cuts at: 1132 1229 2308 2575 2672 4217 1132
Size: 97 1079 267 97 1545 2229
Fragments arranged by size:
2229 1545 1079 267 97 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5314
PciI A'CATG_T
Cuts at: 2571 4213 2571
Size: 1642 3672
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5314
PfoI T'CCnGG_A
Cuts at: 2211 2211
Size: 5314
PmeI GTTT'AAAC
Cuts at: 2618 2618
Size: 5314
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3383 3415 4896 4928 3383
Size: 32 1481 32 3769
Fragments arranged by size:
3769 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2227 2227
Size: 5314
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5314
PsiI TTA'TAA
Cuts at: 2884 2884
Size: 5314
PstI C_TGCA'G
Cuts at: 830 2124 2666 830
Size: 1294 542 3478
Fragments arranged by size:
3478 1294 542
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5314
SacII CC_GC'GG
Cuts at: 2170 2170
Size: 5314
SalI G'TCGA_C
Cuts at: 1936 2656 1936
Size: 720 4594
SbfI CC_TGCA'GG
Cuts at: 2666 2666
Size: 5314
ScaI AGT'ACT
Cuts at: 3562 5274 3562
Size: 1712 3602
SexAI A'CCwGG_T
Cuts at: 2498 2498
Size: 5314
SfcI C'TryA_G
Cuts at: 826 1045 2059 2120 2662 3797 4478 4669 826
Size: 219 1014 61 542 1135 681 191 1471
Fragments arranged by size:
1471 1135 1014 681 542 219 191 61
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5314
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5314
SmaI CCC'GGG
Cuts at: 1872 2374 2641 1872
Size: 502 267 4545
Fragments arranged by size:
4545 502 267
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3418 4319 4581 820
Size: 197 926 1475 901 262 1553
Fragments arranged by size:
1553 1475 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3105 493
Size: 2612 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5314
SphI G_CATG'C
Cuts at: 1132 2672 1132
Size: 1540 3774
SspI AAT'ATT
Cuts at: 5 52 3238 5
Size: 47 3186 2081
Fragments arranged by size:
3186 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5314
StyI C'CwwG_G
Cuts at: 513 1065 2004 2325 2753 513
Size: 552 939 321 428 3074
Fragments arranged by size:
3074 939 552 428 321
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2286 3341 3358 3511 3696 4141 325
Size: 1255 706 1055 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1055 706 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3560 5272 96
Size: 274 80 33 51 1443 1583 1712 138
Fragments arranged by size:
1712 1583 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2138 2170 5274 5306 2138
Size: 32 3104 32 2146
Fragments arranged by size:
3104 2146 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2014 1316
Size: 698 4616
XbaI T'CTAG_A
Cuts at: 2650 2650
Size: 5314
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5314
XmaI C'CCGG_G
Cuts at: 1870 2372 2639 1870
Size: 502 267 4545
Fragments arranged by size:
4545 502 267
XmnI GAAnn'nnTTC
Cuts at: 3443 3443
Size: 5314
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3122 276
Size: 53 83 186 936 1588 2468
Fragments arranged by size:
2468 1588 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SacII SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI
SanDI SapI SfiI SfoI SrfI SwaI XcmI