Restriction map for
pFN21AB8754
(Circular) (Six-base) MAPSORT of: pFN21AB8754.seq Check: 6105 from: 1 to: 5299
pFN21AB8754 5299 bp
With 182 enzymes: SgfI *
February 27, 2009 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2131 2131
Size: 5299
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3109 278
Size: 53 83 186 936 1573 2468
Fragments arranged by size:
2468 1573 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2642 1937
Size: 705 4594
Acc65I G'GTAC_C
Cuts at: 2620 2620
Size: 5299
AclI AA'CG_TT
Cuts at: 1563 3426 3799 1563
Size: 1863 373 3063
Fragments arranged by size:
3063 1863 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3362 4746 5014 1632
Size: 1730 1384 268 1917
Fragments arranged by size:
1917 1730 1384 268
AfeI AGC'GCT
Cuts at: 2546 2546
Size: 5299
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5102
AflIII A'CryG_T
Cuts at: 1203 4198 5175 1203
Size: 2995 977 1327
Fragments arranged by size:
2995 1327 977
AgeI A'CCGG_T
Cuts at: 4130 4130
Size: 5299
AhdI GACnn_n'nnGTC
Cuts at: 4028 4028
Size: 5299
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5299
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5299
AlwNI CAG_nnn'CTG
Cuts at: 2224 2284 2494 3042 4614 2224
Size: 60 210 548 1572 2909
Fragments arranged by size:
2909 1572 548 210 60
ApaI G_GGCC'C
Cuts at: 2301 2301
Size: 5299
ApaLI G'TGCA_C
Cuts at: 2167 3356 4512 2167
Size: 1189 1156 2954
Fragments arranged by size:
2954 1189 1156
ApoI r'AATT_y
Cuts at: 1493 2608 2834 2986 5095 1493
Size: 1115 226 152 2109 1697
Fragments arranged by size:
2109 1697 1115 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3853 160
Size: 872 2821 1606
Fragments arranged by size:
2821 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5299
AvaI C'yCGr_G
Cuts at: 1870 1943 2624 1870
Size: 73 681 4545
Fragments arranged by size:
4545 681 73
BamHI G'GATC_C
Cuts at: 2629 2996 2629
Size: 367 4932
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2620 4075 618
Size: 325 217 627 833 1455 1842
Fragments arranged by size:
1842 1455 833 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2069 2301 2484 2618 1391
Size: 678 232 183 134 4072
Fragments arranged by size:
4072 678 232 183 134
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4328
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3490 3524 1903
Size: 34 50 34 1469 34 3678
Fragments arranged by size:
3678 1469 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3191 4401 3191
Size: 1210 4089
BclI T'GATC_A
Cuts at: 1548 2145 1548
Size: 597 4702
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3910 136
Size: 107 122 71 631 605 2238 1525
Fragments arranged by size:
2238 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5294 5294
Size: 5299
BlpI GC'TnA_GC
Cuts at: 2716 2716
Size: 5299
Bme1580I G_kGCm'C
Cuts at: 1790 2096 2171 2301 3360 4516 1790
Size: 306 75 130 1059 1156 2573
Fragments arranged by size:
2573 1156 1059 306 130 75
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5299
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3030 3988 442
Size: 416 53 83 399 24 1613 958 1753
Fragments arranged by size:
1753 1613 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5299
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2306 2525 3959 1239
Size: 1067 219 1434 2579
Fragments arranged by size:
2579 1434 1067 219
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3424 4289 4587 3424
Size: 865 298 4136
Fragments arranged by size:
4136 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2441 3962 882
Size: 617 942 1521 2219
Fragments arranged by size:
2219 1521 942 617
BsaAI yAC'GTr
Cuts at: 493 1204 3090 493
Size: 711 1886 2702
Fragments arranged by size:
2702 1886 711
BsaBI GATnn'nnATC
Cuts at: 5293 5293
Size: 5299
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3106 3488 4123 275
Size: 53 83 186 936 1573 382 635 1451
Fragments arranged by size:
1573 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3732 4130 4404 4551 4962 3732
Size: 398 274 147 411 4069
Fragments arranged by size:
4069 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2417 2447 1178
Size: 30 165 30 1014 30 4030
Fragments arranged by size:
4030 1014 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2303 1186
Size: 1117 4182
BseYI C'CCAG_C
Cuts at: 2293 4502 4998 2293
Size: 2209 496 2594
Fragments arranged by size:
2594 2209 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2339 1163
Size: 573 603 4123
Fragments arranged by size:
4123 603 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2749 3510 4538 1540
Size: 275 186 748 761 1028 2301
Fragments arranged by size:
2301 1028 761 748 275 186
BsiHKAI G_wGCw'C
Cuts at: 2069 2171 2484 3360 3445 4516 2069
Size: 102 313 876 85 1071 2852
Fragments arranged by size:
2852 1071 876 313 102 85
BsmI GAATG_Cn'
Cuts at: 2810 2903 2810
Size: 93 5206
BsmBI CGTCTCn'nnnn_
Cuts at: 2372 2501 2372
Size: 129 5170
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2069 2096 2171 2301 2484 2618 3360
Size: 399 279 27 75 130 183 134 742
Cuts at: 3360 3445 4516 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 279 183 134 130
85 75 27
BspEI T'CCGG_A
Cuts at: 4962 4962
Size: 5299
BspHI T'CATG_A
Cuts at: 3188 4918 3188
Size: 1730 3569
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2131 2654 844
Size: 1287 523 3489
Fragments arranged by size:
3489 1287 523
BsrBI CCG'CTC
Cuts at: 1394 1823 3186 1394
Size: 429 1363 3507
Fragments arranged by size:
3507 1363 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3794 3968 65
Size: 1183 2546 174 1396
Fragments arranged by size:
2546 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2136 2192 3943 4130 1700
Size: 110 326 56 1751 187 2869
Fragments arranged by size:
2869 1751 326 187 110 56
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5299
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5299
BssSI C'ACGA_G
Cuts at: 2357 3359 4371 2357
Size: 1002 1012 3285
Fragments arranged by size:
3285 1012 1002
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2370 794
Size: 1576 3723
BstBI TT'CG_AA
Cuts at: 4103 4103
Size: 5299
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5299
BstYI r'GATC_y
Cuts at: 1972 2629 2996 3381 3398 4850 4936 4948 5294
Size: 657 367 385 17 1452 86 12 346
Cuts at: 5294 1972
Size: 1977
Fragments arranged by size:
1977 1452 657 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4747
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2388 487
Size: 683 56 1162 3398
Fragments arranged by size:
3398 1162 683 56
BtsI GCAGTG_nn'
Cuts at: 2815 3609 3629 2815
Size: 794 20 4485
Fragments arranged by size:
4485 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5299
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5264
DraI TTT'AAA
Cuts at: 2603 2603
Size: 5299
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4306 809
Size: 507 2990 1802
Fragments arranged by size:
2990 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2211 2454 2746
Size: 54 1425 50 275 18 381 243 292
Cuts at: 2746 3635 5264 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 381 292 275 243 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2746 1537
Size: 275 934 4090
Fragments arranged by size:
4090 934 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2489 2555 3229 1353
Size: 1136 66 674 3423
Fragments arranged by size:
3423 1136 674 66
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3886 4260 4406 387
Size: 1064 138 2297 374 146 1280
Fragments arranged by size:
2297 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2067 2482 2067
Size: 415 4884
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2306 2525 3362 3959 4746 5014 1239
Size: 393 674 219 837 597 787 268 1524
Fragments arranged by size:
1524 837 787 674 597 393 268 219
EcoO109I rG'GnC_Cy
Cuts at: 2297 2297
Size: 5299
EcoRI G'AATT_C
Cuts at: 2608 2986 2608
Size: 378 4921
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2500 2500
Size: 5299
FspI TGC'GCA
Cuts at: 3805 3805
Size: 5299
HaeII r_GCGC'y
Cuts at: 1678 2224 2548 4446 1678
Size: 546 324 1898 2531
Fragments arranged by size:
2531 1898 546 324
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3940 3972 4014 4046 1373
Size: 32 135 32 2368 32 42 32 2626
Fragments arranged by size:
2626 2368 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2643 2889 669
Size: 988 281 705 246 3079
Fragments arranged by size:
3079 988 705 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5299
HpaI GTT'AAC
Cuts at: 2889 2889
Size: 5299
KpnI G_GTAC'C
Cuts at: 2624 2624
Size: 5299
MfeI C'AATT_G
Cuts at: 2898 2898
Size: 5299
MluI A'CGCG_T
Cuts at: 5175 5175
Size: 5299
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4413 4597 1305
Size: 3108 184 2007
Fragments arranged by size:
3108 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3477
Fragments arranged by size:
3477 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2255 2348 2594 3257 3616 3775 518
Size: 648 1089 93 246 663 359 159 2042
Fragments arranged by size:
2042 1089 663 648 359 246 159 93
MspA1I CmG'CkG
Cuts at: 1606 2151 2386 2715 3392 4540 4785 1606
Size: 545 235 329 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 545 329 245 235
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4747
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5299
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5299
NotI GC'GGCC_GC
Cuts at: 2746 2746
Size: 5299
NspI r_CATG'y
Cuts at: 1132 1229 2657 4202 1132
Size: 97 1428 1545 2229
Fragments arranged by size:
2229 1545 1428 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5299
PciI A'CATG_T
Cuts at: 4198 4198
Size: 5299
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5299
PfoI T'CCnGG_A
Cuts at: 2487 2549 2487
Size: 62 5237
PmeI GTTT'AAAC
Cuts at: 2603 2603
Size: 5299
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3368 3400 4881 4913 3368
Size: 32 1481 32 3754
Fragments arranged by size:
3754 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5299
PsiI TTA'TAA
Cuts at: 2869 2869
Size: 5299
PspOMI G'GGCC_C
Cuts at: 2297 2297
Size: 5299
PstI C_TGCA'G
Cuts at: 830 2178 2651 830
Size: 1348 473 3478
Fragments arranged by size:
3478 1348 473
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5299
PvuII CAG'CTG
Cuts at: 2151 2151
Size: 5299
SacI G_AGCT'C
Cuts at: 2069 2484 2069
Size: 415 4884
SalI G'TCGA_C
Cuts at: 1936 2641 1936
Size: 705 4594
SapI GCTCTTCn'nnn_
Cuts at: 2489 2489
Size: 5299
SbfI CC_TGCA'GG
Cuts at: 2651 2651
Size: 5299
ScaI AGT'ACT
Cuts at: 3547 5259 3547
Size: 1712 3587
SfcI C'TryA_G
Cuts at: 826 1045 2174 2647 3782 4463 4654 826
Size: 219 1129 473 1135 681 191 1471
Fragments arranged by size:
1471 1135 1129 681 473 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5299
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5299
SmaI CCC'GGG
Cuts at: 1872 2626 1872
Size: 754 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3403 4304 4566 820
Size: 197 926 1460 901 262 1553
Fragments arranged by size:
1553 1460 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3090 493
Size: 2597 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5299
SphI G_CATG'C
Cuts at: 1132 2657 1132
Size: 1525 3774
SspI AAT'ATT
Cuts at: 5 52 3223 5
Size: 47 3171 2081
Fragments arranged by size:
3171 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5299
StyI C'CwwG_G
Cuts at: 513 1065 2235 2738 513
Size: 552 1170 503 3074
Fragments arranged by size:
3074 1170 552 503
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3326 3343 3496 3681 4126 325
Size: 1255 1746 17 153 185 445 1498
Fragments arranged by size:
1746 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3545 5257 96
Size: 274 80 33 51 1443 1568 1712 138
Fragments arranged by size:
1712 1568 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2417 2449 5259 5291 2417
Size: 32 2810 32 2425
Fragments arranged by size:
2810 2425 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2573 1316
Size: 1257 4042
XbaI T'CTAG_A
Cuts at: 2635 2635
Size: 5299
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5299
XmaI C'CCGG_G
Cuts at: 1870 2624 1870
Size: 754 4545
XmnI GAAnn'nnTTC
Cuts at: 3428 3428
Size: 5299
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3107 276
Size: 53 83 186 936 1573 2468
Fragments arranged by size:
2468 1573 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI
SexAI SfiI SfoI SrfI SwaI XcmI