Restriction map for
pFN21AB8747
(Circular) (Six-base) MAPSORT of: pFN21AB8747.seq Check: 5245 from: 1 to: 4798
pFN21AB8747 4798 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2608 278
Size: 53 83 186 936 1072 2468
Fragments arranged by size:
2468 1072 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2141 1937
Size: 204 4594
Acc65I G'GTAC_C
Cuts at: 2119 2119
Size: 4798
AclI AA'CG_TT
Cuts at: 1563 2925 3298 1563
Size: 1362 373 3063
Fragments arranged by size:
3063 1362 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2861 4245 4513 1632
Size: 1229 1384 268 1917
Fragments arranged by size:
1917 1384 1229 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4601
AflIII A'CryG_T
Cuts at: 1203 3697 4674 1203
Size: 2494 977 1327
Fragments arranged by size:
2494 1327 977
AgeI A'CCGG_T
Cuts at: 3629 3629
Size: 4798
AhdI GACnn_n'nnGTC
Cuts at: 3527 3527
Size: 4798
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 4798
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 4798
AlwNI CAG_nnn'CTG
Cuts at: 2541 4113 2541
Size: 1572 3226
ApaLI G'TGCA_C
Cuts at: 2855 4011 2855
Size: 1156 3642
ApoI r'AATT_y
Cuts at: 1493 2107 2333 2485 4594 1493
Size: 614 226 152 2109 1697
Fragments arranged by size:
2109 1697 614 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3352 160
Size: 872 2320 1606
Fragments arranged by size:
2320 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4798
AvaI C'yCGr_G
Cuts at: 1870 1943 2019 2123 1870
Size: 73 76 104 4545
Fragments arranged by size:
4545 104 76 73
BamHI G'GATC_C
Cuts at: 2128 2495 2128
Size: 367 4431
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2119 3574 618
Size: 325 217 627 332 1455 1842
Fragments arranged by size:
1842 1455 627 332 325 217
BanII G_rGCy'C
Cuts at: 1391 2117 1391
Size: 726 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 3827
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2989 3023 1903
Size: 34 1052 34 3678
Fragments arranged by size:
3678 1052 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2690 3900 2690
Size: 1210 3588
BclI T'GATC_A
Cuts at: 1548 1548
Size: 4798
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3409 136
Size: 107 122 71 631 605 1737 1525
Fragments arranged by size:
1737 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4793 4793
Size: 4798
BlpI GC'TnA_GC
Cuts at: 2215 2215
Size: 4798
Bme1580I G_kGCm'C
Cuts at: 1790 2859 4015 1790
Size: 1069 1156 2573
Fragments arranged by size:
2573 1156 1069
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 4798
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2529 3487 442
Size: 416 53 83 399 24 1112 958 1753
Fragments arranged by size:
1753 1112 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 4798
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3458 1239
Size: 2219 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2923 3788 4086 2923
Size: 865 298 3635
Fragments arranged by size:
3635 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3461 882
Size: 617 1962 2219
Fragments arranged by size:
2219 1962 617
BsaAI yAC'GTr
Cuts at: 493 1204 2589 493
Size: 711 1385 2702
Fragments arranged by size:
2702 1385 711
BsaBI GATnn'nnATC
Cuts at: 4792 4792
Size: 4798
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2605 2987 3622 275
Size: 53 83 186 936 1072 382 635 1451
Fragments arranged by size:
1451 1072 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3231 3629 3903 4050 4461 3231
Size: 398 274 147 411 3568
Fragments arranged by size:
3568 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4573
Fragments arranged by size:
4573 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 4798
BseYI C'CCAG_C
Cuts at: 4001 4497 4001
Size: 496 4302
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4225
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2248 3009 4037 1540
Size: 275 186 247 761 1028 2301
Fragments arranged by size:
2301 1028 761 275 247 186
BsiHKAI G_wGCw'C
Cuts at: 2859 2944 4015 2859
Size: 85 1071 3642
Fragments arranged by size:
3642 1071 85
BsmI GAATG_Cn'
Cuts at: 2309 2402 2309
Size: 93 4705
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2117 2859 2944 4015 1391
Size: 399 327 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 327 85
BspEI T'CCGG_A
Cuts at: 4461 4461
Size: 4798
BspHI T'CATG_A
Cuts at: 2687 4417 2687
Size: 1730 3068
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2153 844
Size: 1309 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2685 1394
Size: 429 862 3507
Fragments arranged by size:
3507 862 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3293 3467 65
Size: 1183 2045 174 1396
Fragments arranged by size:
2045 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3442 3629 1700
Size: 110 1632 187 2869
Fragments arranged by size:
2869 1632 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 4798
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 4798
BssSI C'ACGA_G
Cuts at: 2858 3870 2858
Size: 1012 3786
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 4798
BstBI TT'CG_AA
Cuts at: 3602 3602
Size: 4798
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 4798
BstYI r'GATC_y
Cuts at: 1972 2128 2495 2880 2897 4349 4435 4447 4793
Size: 156 367 385 17 1452 86 12 346
Cuts at: 4793 1972
Size: 1977
Fragments arranged by size:
1977 1452 385 367 346 156 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3307
Fragments arranged by size:
3307 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4059
Fragments arranged by size:
4059 683 56
BtsI GCAGTG_nn'
Cuts at: 2314 3108 3128 2314
Size: 794 20 3984
Fragments arranged by size:
3984 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 4798
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 4763
DraI TTT'AAA
Cuts at: 2102 2102
Size: 4798
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3805 809
Size: 507 2489 1802
Fragments arranged by size:
2489 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2245 3134 4763
Size: 54 1425 50 275 18 415 889 1629
Cuts at: 4763 8
Size: 43
Fragments arranged by size:
1629 1425 889 415 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2245 1537
Size: 275 433 4090
Fragments arranged by size:
4090 433 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2728 1353
Size: 1375 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3385 3759 3905 387
Size: 1064 138 1796 374 146 1280
Fragments arranged by size:
1796 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2861 3458 4245 4513 1239
Size: 393 1229 597 787 268 1524
Fragments arranged by size:
1524 1229 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2085 2085
Size: 4798
EcoRI G'AATT_C
Cuts at: 2107 2485 2107
Size: 378 4420
FspI TGC'GCA
Cuts at: 3304 3304
Size: 4798
HaeII r_GCGC'y
Cuts at: 1678 3945 1678
Size: 2267 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2074 2106 3439 3471 3513
Size: 32 135 32 502 32 1333 32 42
Cuts at: 3513 3545 1373
Size: 32 2626
Fragments arranged by size:
2626 1333 502 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2142 2388 669
Size: 988 281 204 246 3079
Fragments arranged by size:
3079 988 281 246 204
HindIII A'AGCT_T
Cuts at: 748 748
Size: 4798
HpaI GTT'AAC
Cuts at: 2388 2388
Size: 4798
KpnI G_GTAC'C
Cuts at: 2123 2123
Size: 4798
MfeI C'AATT_G
Cuts at: 2397 2397
Size: 4798
MluI A'CGCG_T
Cuts at: 4674 4674
Size: 4798
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3912 4096 1305
Size: 2607 184 2007
Fragments arranged by size:
2607 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 2976
Fragments arranged by size:
2976 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2756 3115 3274 518
Size: 648 1590 359 159 2042
Fragments arranged by size:
2042 1590 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2214 2891 4039 4284 1606
Size: 608 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 608 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3307
Fragments arranged by size:
3307 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 4798
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 4798
NotI GC'GGCC_GC
Cuts at: 2245 2245
Size: 4798
NspI r_CATG'y
Cuts at: 1132 1229 2156 3701 1132
Size: 97 927 1545 2229
Fragments arranged by size:
2229 1545 927 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 4798
PciI A'CATG_T
Cuts at: 3697 3697
Size: 4798
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 4798
PmeI GTTT'AAAC
Cuts at: 2102 2102
Size: 4798
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2867 2899 4380 4412 2867
Size: 32 1481 32 3253
Fragments arranged by size:
3253 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2085 2085
Size: 4798
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 4798
PsiI TTA'TAA
Cuts at: 2368 2368
Size: 4798
PstI C_TGCA'G
Cuts at: 830 2150 830
Size: 1320 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 4798
SalI G'TCGA_C
Cuts at: 1936 2140 1936
Size: 204 4594
SbfI CC_TGCA'GG
Cuts at: 2150 2150
Size: 4798
ScaI AGT'ACT
Cuts at: 3046 4758 3046
Size: 1712 3086
SfcI C'TryA_G
Cuts at: 826 1045 2146 3281 3962 4153 826
Size: 219 1101 1135 681 191 1471
Fragments arranged by size:
1471 1135 1101 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4798
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 4798
SmaI CCC'GGG
Cuts at: 1872 2021 2125 1872
Size: 149 104 4545
Fragments arranged by size:
4545 149 104
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2902 3803 4065 820
Size: 197 926 959 901 262 1553
Fragments arranged by size:
1553 959 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2589 493
Size: 2096 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 4798
SphI G_CATG'C
Cuts at: 1132 2156 1132
Size: 1024 3774
SspI AAT'ATT
Cuts at: 5 52 2722 5
Size: 47 2670 2081
Fragments arranged by size:
2670 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 4798
StyI C'CwwG_G
Cuts at: 513 1065 2004 2237 513
Size: 552 939 233 3074
Fragments arranged by size:
3074 939 552 233
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2825 2842 2995 3180 3625 325
Size: 1255 1245 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1245 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3044 4756 96
Size: 274 80 33 51 1443 1067 1712 138
Fragments arranged by size:
1712 1443 1067 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4758 4790 4758
Size: 32 4766
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 4798
XbaI T'CTAG_A
Cuts at: 2134 2134
Size: 4798
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 4798
XmaI C'CCGG_G
Cuts at: 1870 2019 2123 1870
Size: 149 104 4545
Fragments arranged by size:
4545 149 104
XmnI GAAnn'nnTTC
Cuts at: 2927 2927
Size: 4798
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2606 276
Size: 53 83 186 936 1072 2468
Fragments arranged by size:
2468 1072 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI