Restriction map for
pFN21AB8745
(Circular) (Six-base) MAPSORT of: pFN21AB8745.seq Check: 7014 from: 1 to: 5911
pFN21AB8745 5911 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3721 278
Size: 53 83 186 936 2185 2468
Fragments arranged by size:
2468 2185 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3254 1937
Size: 1317 4594
Acc65I G'GTAC_C
Cuts at: 3232 3232
Size: 5911
AclI AA'CG_TT
Cuts at: 1563 4038 4411 1563
Size: 2475 373 3063
Fragments arranged by size:
3063 2475 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2291 3974 5358 5626 1632
Size: 659 1683 1384 268 1917
Fragments arranged by size:
1917 1683 1384 659 268
AfeI AGC'GCT
Cuts at: 2258 2258
Size: 5911
AflII C'TTAA_G
Cuts at: 820 1017 2579 820
Size: 197 1562 4152
Fragments arranged by size:
4152 1562 197
AflIII A'CryG_T
Cuts at: 1203 2023 4810 5787 1203
Size: 820 2787 977 1327
Fragments arranged by size:
2787 1327 977 820
AgeI A'CCGG_T
Cuts at: 4742 4742
Size: 5911
AhdI GACnn_n'nnGTC
Cuts at: 4640 4640
Size: 5911
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5911
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5911
AlwNI CAG_nnn'CTG
Cuts at: 3142 3654 5226 3142
Size: 512 1572 3827
Fragments arranged by size:
3827 1572 512
ApaI G_GGCC'C
Cuts at: 2071 2282 2071
Size: 211 5700
ApaLI G'TGCA_C
Cuts at: 3968 5124 3968
Size: 1156 4755
ApoI r'AATT_y
Cuts at: 1493 2402 3118 3220 3446 3598 5707 1493
Size: 909 716 102 226 152 2109 1697
Fragments arranged by size:
2109 1697 909 716 226 152 102
AseI AT'TA_AT
Cuts at: 160 1032 4465 160
Size: 872 3433 1606
Fragments arranged by size:
3433 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5911
AvaI C'yCGr_G
Cuts at: 1870 1943 2149 3236 1870
Size: 73 206 1087 4545
Fragments arranged by size:
4545 1087 206 73
BamHI G'GATC_C
Cuts at: 3241 3608 3241
Size: 367 5544
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2161 2876 3232 4687 618
Size: 325 217 627 374 715 356 1455 1842
Fragments arranged by size:
1842 1455 715 627 374 356 325 217
BanII G_rGCy'C
Cuts at: 1391 2071 2282 2756 2953 3230 1391
Size: 680 211 474 197 277 4072
Fragments arranged by size:
4072 680 474 277 211 197
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4940
BbvCI CC'TCA_GC
Cuts at: 2081 2081
Size: 5911
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4102 4136 1903
Size: 34 2165 34 3678
Fragments arranged by size:
3678 2165 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3803 5013 3803
Size: 1210 4701
BclI T'GATC_A
Cuts at: 1548 3123 1548
Size: 1575 4336
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2123 4522 136
Size: 107 122 71 631 605 451 2399 1525
Fragments arranged by size:
2399 1525 631 605 451 122 107 71
BglII A'GATC_T
Cuts at: 5906 5906
Size: 5911
BlpI GC'TnA_GC
Cuts at: 3328 3328
Size: 5911
Bme1580I G_kGCm'C
Cuts at: 1790 2071 2282 2881 3972 5128 1790
Size: 281 211 599 1091 1156 2573
Fragments arranged by size:
2573 1156 1091 599 281 211
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5911
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3642 4600 442
Size: 416 53 83 399 24 2225 958 1753
Fragments arranged by size:
2225 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2013 1056
Size: 957 4954
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2455 2455
Size: 5911
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2456 2654 3119 4571 1239
Size: 1217 198 465 1452 2579
Fragments arranged by size:
2579 1452 1217 465 198
Bpu10I CC'TnA_GC
Cuts at: 2081 2081
Size: 5911
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2237 2409 4036 4901 5199 2237
Size: 172 1627 865 298 2949
Fragments arranged by size:
2949 1627 865 298 172
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4574 882
Size: 617 3075 2219
Fragments arranged by size:
3075 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2024 3702 493
Size: 711 820 1678 2702
Fragments arranged by size:
2702 1678 820 711
BsaBI GATnn'nnATC
Cuts at: 2768 5905 2768
Size: 3137 2774
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2214 3718 4100 4735
Size: 53 83 186 936 681 1504 382 635
Cuts at: 4735 275
Size: 1451
Fragments arranged by size:
1504 1451 936 681 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2517 4344 4742 5016 5163 5574 2517
Size: 1827 398 274 147 411 2854
Fragments arranged by size:
2854 1827 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5686
Fragments arranged by size:
5686 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2255 1186
Size: 1069 4842
BseYI C'CCAG_C
Cuts at: 5114 5610 5114
Size: 496 5415
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2486 1163
Size: 573 750 4588
Fragments arranged by size:
4588 750 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3361 4122 5150 1540
Size: 275 186 1360 761 1028 2301
Fragments arranged by size:
2301 1360 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3972 4057 5128 3972
Size: 85 1071 4755
Fragments arranged by size:
4755 1071 85
BsmI GAATG_Cn'
Cuts at: 2053 3422 3515 2053
Size: 1369 93 4449
Fragments arranged by size:
4449 1369 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2071 2282 2756 2881 2953 3230 3972
Size: 399 281 211 474 125 72 277 742
Cuts at: 3972 4057 5128 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 474 399 281 277 211
125 85 72
BspEI T'CCGG_A
Cuts at: 5574 5574
Size: 5911
BspHI T'CATG_A
Cuts at: 3800 5530 3800
Size: 1730 4181
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3266 844
Size: 2422 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2617 3798 1394
Size: 429 794 1181 3507
Fragments arranged by size:
3507 1181 794 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2016 2794 4406 4580 65
Size: 1183 768 778 1612 174 1396
Fragments arranged by size:
1612 1396 1183 778 768 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2037 4555 4742 1700
Size: 110 227 2518 187 2869
Fragments arranged by size:
2869 2518 227 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5911
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5911
BssSI C'ACGA_G
Cuts at: 3971 4983 3971
Size: 1012 4899
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5911
BstBI TT'CG_AA
Cuts at: 4715 4715
Size: 5911
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5911
BstXI CCAn_nnnn'nTGG
Cuts at: 2210 2359 2632 2210
Size: 149 273 5489
Fragments arranged by size:
5489 273 149
BstYI r'GATC_y
Cuts at: 1972 3241 3608 3993 4010 5462 5548 5560 5906
Size: 1269 367 385 17 1452 86 12 346
Cuts at: 5906 1972
Size: 1977
Fragments arranged by size:
1977 1452 1269 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2273 2907 2273
Size: 634 5277
BtgI C'CryG_G
Cuts at: 513 1065 2004 2395 513
Size: 552 939 391 4029
Fragments arranged by size:
4029 939 552 391
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5172
Fragments arranged by size:
5172 683 56
BtsI GCAGTG_nn'
Cuts at: 2389 2782 3427 4221 4241 2389
Size: 393 645 794 20 4059
Fragments arranged by size:
4059 794 645 393 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5911
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5876
DraI TTT'AAA
Cuts at: 3215 3215
Size: 5911
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4918 809
Size: 507 3602 1802
Fragments arranged by size:
3602 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2115 3358 4247
Size: 54 1425 50 275 18 285 1243 889
Cuts at: 4247 5876 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1243 889 285 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3358 1537
Size: 275 1546 4090
Fragments arranged by size:
4090 1546 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2655 3841 1353
Size: 1302 1186 3423
Fragments arranged by size:
3423 1302 1186
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4498 4872 5018 387
Size: 1064 138 2909 374 146 1280
Fragments arranged by size:
2909 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2291 2456 2654 3119 3974 4571 5358
Size: 393 659 165 198 465 855 597 787
Cuts at: 5358 5626 1239
Size: 268 1524
Fragments arranged by size:
1524 855 787 659 597 465 393 268
198 165
EcoO109I rG'GnC_Cy
Cuts at: 2085 2278 2279 2563 2854 2085
Size: 193 1 284 291 5142
Fragments arranged by size:
5142 291 284 193 1
EcoRI G'AATT_C
Cuts at: 3220 3598 3220
Size: 378 5533
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2459 2459
Size: 5911
FspI TGC'GCA
Cuts at: 4417 4417
Size: 5911
HaeII r_GCGC'y
Cuts at: 1678 2260 5058 1678
Size: 582 2798 2531
Fragments arranged by size:
2798 2531 582
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3115 3147 4552 4584 4626
Size: 32 135 32 1543 32 1405 32 42
Cuts at: 4626 4658 1373
Size: 32 2626
Fragments arranged by size:
2626 1543 1405 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3255 3501 669
Size: 988 281 1317 246 3079
Fragments arranged by size:
3079 1317 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5911
HpaI GTT'AAC
Cuts at: 3501 3501
Size: 5911
KpnI G_GTAC'C
Cuts at: 3236 3236
Size: 5911
MfeI C'AATT_G
Cuts at: 3510 3510
Size: 5911
MluI A'CGCG_T
Cuts at: 5787 5787
Size: 5911
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2593 5025 5209 1305
Size: 1288 2432 184 2007
Fragments arranged by size:
2432 2007 1288 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2117 10
Size: 54 1425 343 285 3804
Fragments arranged by size:
3804 1425 343 285 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2300 3869 4228 4387 518
Size: 648 1134 1569 359 159 2042
Fragments arranged by size:
2042 1569 1134 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3042 3327 4004 5152 5397 1606
Size: 1436 285 677 1148 245 2120
Fragments arranged by size:
2120 1436 1148 677 285 245
NaeI GCC'GGC
Cuts at: 2039 2039
Size: 5911
NcoI C'CATG_G
Cuts at: 513 1065 2004 2395 513
Size: 552 939 391 4029
Fragments arranged by size:
4029 939 552 391
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5911
NgoMIV G'CCGG_C
Cuts at: 2037 2037
Size: 5911
NheI G'CTAG_C
Cuts at: 1052 2009 1052
Size: 957 4954
NotI GC'GGCC_GC
Cuts at: 3358 3358
Size: 5911
NspI r_CATG'y
Cuts at: 1132 1229 3269 4814 1132
Size: 97 2040 1545 2229
Fragments arranged by size:
2229 2040 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2089 1348
Size: 741 5170
PciI A'CATG_T
Cuts at: 4810 4810
Size: 5911
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5911
PfoI T'CCnGG_A
Cuts at: 3135 3135
Size: 5911
PmeI GTTT'AAAC
Cuts at: 3215 3215
Size: 5911
PmlI CAC'GTG
Cuts at: 2024 2024
Size: 5911
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3980 4012 5493 5525 3980
Size: 32 1481 32 4366
Fragments arranged by size:
4366 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2085 2563 2854 2085
Size: 478 291 5142
Fragments arranged by size:
5142 478 291
PshAI GACnn'nnGTC
Cuts at: 1613 2204 1613
Size: 591 5320
PsiI TTA'TAA
Cuts at: 3481 3481
Size: 5911
PspOMI G'GGCC_C
Cuts at: 2067 2278 2067
Size: 211 5700
PstI C_TGCA'G
Cuts at: 830 3263 830
Size: 2433 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5911
SalI G'TCGA_C
Cuts at: 1936 3253 1936
Size: 1317 4594
SbfI CC_TGCA'GG
Cuts at: 3263 3263
Size: 5911
ScaI AGT'ACT
Cuts at: 4159 5871 4159
Size: 1712 4199
SfcI C'TryA_G
Cuts at: 826 1045 3259 4394 5075 5266 826
Size: 219 2214 1135 681 191 1471
Fragments arranged by size:
2214 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5911
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5911
SmaI CCC'GGG
Cuts at: 1872 3238 1872
Size: 1366 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2252 2424 2579 4015 4916 5178
Size: 197 926 309 172 155 1436 901 262
Cuts at: 5178 820
Size: 1553
Fragments arranged by size:
1553 1436 926 901 309 262 197 172
155
SnaBI TAC'GTA
Cuts at: 493 3702 493
Size: 3209 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5911
SphI G_CATG'C
Cuts at: 1132 3269 1132
Size: 2137 3774
SspI AAT'ATT
Cuts at: 5 52 3835 5
Size: 47 3783 2081
Fragments arranged by size:
3783 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5911
StyI C'CwwG_G
Cuts at: 513 1065 2004 2063 2170 2395 3350 513
Size: 552 939 59 107 225 955 3074
Fragments arranged by size:
3074 955 939 552 225 107 59
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2093 3938 3955 4108 4293 4738 325
Size: 1255 513 1845 17 153 185 445 1498
Fragments arranged by size:
1845 1498 1255 513 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4157 5869 96
Size: 274 80 33 51 1443 2180 1712 138
Fragments arranged by size:
2180 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5871 5903 5871
Size: 32 5879
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5911
XbaI T'CTAG_A
Cuts at: 3247 3247
Size: 5911
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2868 2868
Size: 5911
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5911
XmaI C'CCGG_G
Cuts at: 1870 3236 1870
Size: 1366 4545
XmnI GAAnn'nnTTC
Cuts at: 2144 4040 2144
Size: 1896 4015
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3719 276
Size: 53 83 186 936 2185 2468
Fragments arranged by size:
2468 2185 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI
KasI NarI NruI NsiI PacI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI