Restriction map for
pFN21AB8737
(Circular) (Six-base) MAPSORT of: pFN21AB8737.seq Check: 7302 from: 1 to: 5134
pFN21AB8737 5134 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2262 2262
Size: 5134
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2944 278
Size: 53 83 186 936 1408 2468
Fragments arranged by size:
2468 1408 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2477 1937
Size: 540 4594
Acc65I G'GTAC_C
Cuts at: 2455 2455
Size: 5134
AclI AA'CG_TT
Cuts at: 1563 3261 3634 1563
Size: 1698 373 3063
Fragments arranged by size:
3063 1698 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3197 4581 4849 1632
Size: 1565 1384 268 1917
Fragments arranged by size:
1917 1565 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4937
AflIII A'CryG_T
Cuts at: 1203 4033 5010 1203
Size: 2830 977 1327
Fragments arranged by size:
2830 1327 977
AgeI A'CCGG_T
Cuts at: 3965 3965
Size: 5134
AhdI GACnn_n'nnGTC
Cuts at: 3863 3863
Size: 5134
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5134
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5134
AlwNI CAG_nnn'CTG
Cuts at: 2877 4449 2877
Size: 1572 3562
ApaLI G'TGCA_C
Cuts at: 3191 4347 3191
Size: 1156 3978
ApoI r'AATT_y
Cuts at: 1493 2443 2669 2821 4930 1493
Size: 950 226 152 2109 1697
Fragments arranged by size:
2109 1697 950 226 152
AscI GG'CGCG_CC
Cuts at: 2145 2145
Size: 5134
AseI AT'TA_AT
Cuts at: 160 1032 3688 160
Size: 872 2656 1606
Fragments arranged by size:
2656 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5134
AvaI C'yCGr_G
Cuts at: 1870 1943 2395 2459 1870
Size: 73 452 64 4545
Fragments arranged by size:
4545 452 73 64
BamHI G'GATC_C
Cuts at: 2464 2831 2464
Size: 367 4767
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2138 2389 2455 3910 618
Size: 325 217 627 351 251 66 1455 1842
Fragments arranged by size:
1842 1455 627 351 325 251 217 66
BanII G_rGCy'C
Cuts at: 1391 2286 2453 1391
Size: 895 167 4072
Fragments arranged by size:
4072 895 167
BbeI G_GCGC'C
Cuts at: 2142 2393 2142
Size: 251 4883
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2237 2369 928
Size: 971 338 132 3693
Fragments arranged by size:
3693 971 338 132
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3325 3359 1903
Size: 34 1388 34 3678
Fragments arranged by size:
3678 1388 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3026 4236 3026
Size: 1210 3924
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5134
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2417 3745 136
Size: 107 122 71 631 605 745 1328 1525
Fragments arranged by size:
1525 1328 745 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5129 5129
Size: 5134
BlpI GC'TnA_GC
Cuts at: 2551 2551
Size: 5134
Bme1580I G_kGCm'C
Cuts at: 1790 3195 4351 1790
Size: 1405 1156 2573
Fragments arranged by size:
2573 1405 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5134
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2078 2865 3823
Size: 416 53 83 399 24 661 787 958
Cuts at: 3823 442
Size: 1753
Fragments arranged by size:
1753 958 787 661 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5134
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2060 2192 2216 3794 1239
Size: 821 132 24 1578 2579
Fragments arranged by size:
2579 1578 821 132 24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3259 4124 4422 3259
Size: 865 298 3971
Fragments arranged by size:
3971 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3797 882
Size: 617 2298 2219
Fragments arranged by size:
2298 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2925 493
Size: 711 1721 2702
Fragments arranged by size:
2702 1721 711
BsaBI GATnn'nnATC
Cuts at: 5128 5128
Size: 5134
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2139 2325 2390 2941
Size: 53 83 186 936 606 186 65 551
Cuts at: 2941 3323 3958 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 635 606 551 382 186 186
83 65 53
BsaWI w'CCGG_w
Cuts at: 3567 3965 4239 4386 4797 3567
Size: 398 274 147 411 3904
Fragments arranged by size:
3904 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4909
Fragments arranged by size:
4909 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2294 1186
Size: 1108 4026
BseYI C'CCAG_C
Cuts at: 2296 4337 4833 2296
Size: 2041 496 2597
Fragments arranged by size:
2597 2041 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4561
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2313 2584 3345 4373 1540
Size: 275 186 312 271 761 1028 2301
Fragments arranged by size:
2301 1028 761 312 275 271 186
BsiHKAI G_wGCw'C
Cuts at: 2286 3195 3280 4351 2286
Size: 909 85 1071 3069
Fragments arranged by size:
3069 1071 909 85
BsmI GAATG_Cn'
Cuts at: 2645 2738 2645
Size: 93 5041
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2286 2453 3195 3280 4351 1391
Size: 399 496 167 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 496 399 167 85
BspEI T'CCGG_A
Cuts at: 4797 4797
Size: 5134
BspHI T'CATG_A
Cuts at: 3023 4753 3023
Size: 1730 3404
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2262 2489 844
Size: 1418 227 3489
Fragments arranged by size:
3489 1418 227
BsrBI CCG'CTC
Cuts at: 1394 1823 3021 1394
Size: 429 1198 3507
Fragments arranged by size:
3507 1198 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2215 3629 3803 65
Size: 1183 967 1414 174 1396
Fragments arranged by size:
1414 1396 1183 967 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2069 2100 2141 3778 3965 1700
Size: 110 259 31 41 1637 187 2869
Fragments arranged by size:
2869 1637 259 187 110 41 31
BsrGI T'GTAC_A
Cuts at: 96 2095 96
Size: 1999 3135
BssHII G'CGCG_C
Cuts at: 1925 2065 2145 1925
Size: 140 80 4914
Fragments arranged by size:
4914 140 80
BssSI C'ACGA_G
Cuts at: 3194 4206 3194
Size: 1012 4122
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5134
BstBI TT'CG_AA
Cuts at: 3938 3938
Size: 5134
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5134
BstYI r'GATC_y
Cuts at: 1972 2190 2464 2831 3216 3233 4685 4771 4783
Size: 218 274 367 385 17 1452 86 12
Cuts at: 4783 5129 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 346 274 218 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2014 2376 513
Size: 552 949 362 3271
Fragments arranged by size:
3271 949 552 362
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4395
Fragments arranged by size:
4395 683 56
BtsI GCAGTG_nn'
Cuts at: 2172 2650 3444 3464 2172
Size: 478 794 20 3842
Fragments arranged by size:
3842 794 478 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5134
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5099
DraI TTT'AAA
Cuts at: 2438 2438
Size: 5134
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4141 809
Size: 507 2825 1802
Fragments arranged by size:
2825 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2262 2581 3470
Size: 54 1425 50 275 18 432 319 889
Cuts at: 3470 5099 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 432 319 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2581 1537
Size: 275 769 4090
Fragments arranged by size:
4090 769 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3064 1353
Size: 1711 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3721 4095 4241 387
Size: 1064 138 2132 374 146 1280
Fragments arranged by size:
2132 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2284 2284
Size: 5134
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2060 2192 2216 3197 3794 4581 4849
Size: 393 428 132 24 981 597 787 268
Cuts at: 4849 1239
Size: 1524
Fragments arranged by size:
1524 981 787 597 428 393 268 132
24
EcoRI G'AATT_C
Cuts at: 2443 2821 2443
Size: 378 4756
FspI TGC'GCA
Cuts at: 3640 3640
Size: 5134
HaeII r_GCGC'y
Cuts at: 1678 2142 2373 2393 4281 1678
Size: 464 231 20 1888 2531
Fragments arranged by size:
2531 1888 464 231 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3775 3807 3849 3881 1373
Size: 32 135 32 2203 32 42 32 2626
Fragments arranged by size:
2626 2203 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2478 2724 669
Size: 988 281 540 246 3079
Fragments arranged by size:
3079 988 540 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5134
HpaI GTT'AAC
Cuts at: 2724 2724
Size: 5134
KasI G'GCGC_C
Cuts at: 2138 2389 2138
Size: 251 4883
KpnI G_GTAC'C
Cuts at: 2459 2459
Size: 5134
MfeI C'AATT_G
Cuts at: 2733 2733
Size: 5134
MluI A'CGCG_T
Cuts at: 5010 5010
Size: 5134
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4248 4432 1305
Size: 2943 184 2007
Fragments arranged by size:
2943 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2264 10
Size: 54 1425 343 432 2880
Fragments arranged by size:
2880 1425 432 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3092 3451 3610 518
Size: 648 1926 359 159 2042
Fragments arranged by size:
2042 1926 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2016 2186 2260 2550 3227 4375 4620 1606
Size: 410 170 74 290 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 410 290 245 170 74
NaeI GCC'GGC
Cuts at: 2071 2143 2071
Size: 72 5062
NarI GG'CG_CC
Cuts at: 2139 2390 2139
Size: 251 4883
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4582
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5134
NgoMIV G'CCGG_C
Cuts at: 2069 2141 2069
Size: 72 5062
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5134
NotI GC'GGCC_GC
Cuts at: 2581 2581
Size: 5134
NspI r_CATG'y
Cuts at: 1132 1229 2492 4037 1132
Size: 97 1263 1545 2229
Fragments arranged by size:
2229 1545 1263 97
PasI CC'CwG_GG
Cuts at: 1348 2317 1348
Size: 969 4165
PciI A'CATG_T
Cuts at: 4033 4033
Size: 5134
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5134
PfoI T'CCnGG_A
Cuts at: 2193 2193
Size: 5134
PmeI GTTT'AAAC
Cuts at: 2438 2438
Size: 5134
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3203 3235 4716 4748 3203
Size: 32 1481 32 3589
Fragments arranged by size:
3589 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5134
PsiI TTA'TAA
Cuts at: 2704 2704
Size: 5134
PstI C_TGCA'G
Cuts at: 830 2259 2486 830
Size: 1429 227 3478
Fragments arranged by size:
3478 1429 227
PvuI CG_AT'CG
Cuts at: 2001 2313 2001
Size: 312 4822
PvuII CAG'CTG
Cuts at: 2260 2260
Size: 5134
SacI G_AGCT'C
Cuts at: 2286 2286
Size: 5134
SacII CC_GC'GG
Cuts at: 2017 2017
Size: 5134
SalI G'TCGA_C
Cuts at: 1936 2476 1936
Size: 540 4594
SbfI CC_TGCA'GG
Cuts at: 2486 2486
Size: 5134
ScaI AGT'ACT
Cuts at: 3382 5094 3382
Size: 1712 3422
SfcI C'TryA_G
Cuts at: 826 1045 2255 2482 3617 4298 4489 826
Size: 219 1210 227 1135 681 191 1471
Fragments arranged by size:
1471 1210 1135 681 227 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2417 2417
Size: 5134
SfoI GGC'GCC
Cuts at: 2140 2391 2140
Size: 251 4883
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5134
SgrAI Cr'CCGG_yG
Cuts at: 1700 2141 1700
Size: 441 4693
SmaI CCC'GGG
Cuts at: 1872 2461 1872
Size: 589 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3238 4139 4401 820
Size: 197 926 1295 901 262 1553
Fragments arranged by size:
1553 1295 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2925 493
Size: 2432 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5134
SphI G_CATG'C
Cuts at: 1132 2492 1132
Size: 1360 3774
SspI AAT'ATT
Cuts at: 5 52 3058 5
Size: 47 3006 2081
Fragments arranged by size:
3006 2081 47
StuI AGG'CCT
Cuts at: 1517 2420 1517
Size: 903 4231
StyI C'CwwG_G
Cuts at: 513 1065 2043 2573 513
Size: 552 978 530 3074
Fragments arranged by size:
3074 978 552 530
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2429 3161 3178 3331 3516 3961 325
Size: 1255 849 732 17 153 185 445 1498
Fragments arranged by size:
1498 1255 849 732 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2095 3380 5092
Size: 274 80 33 51 1443 118 1285 1712
Cuts at: 5092 96
Size: 138
Fragments arranged by size:
1712 1443 1285 274 138 118 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5094 5126 5094
Size: 32 5102
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5134
XbaI T'CTAG_A
Cuts at: 2470 2470
Size: 5134
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2374 2374
Size: 5134
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5134
XmaI C'CCGG_G
Cuts at: 1870 2459 1870
Size: 589 4545
XmnI GAAnn'nnTTC
Cuts at: 3263 3263
Size: 5134
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2942 276
Size: 53 83 186 936 1408 2468
Fragments arranged by size:
2468 1408 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SacI SacII SalI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI
EcoO109I EcoRV FalI FseI FspAI NruI NsiI PacI
PmlI PpuMI PspOMI PspXI PsrI RsrII SanDI SapI
SexAI SrfI SwaI