Restriction map for
pFN21AB8736
(Circular) (Six-base) MAPSORT of: pFN21AB8736.seq Check: 2710 from: 1 to: 5905
pFN21AB8736 5905 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3715 278
Size: 53 83 186 936 2179 2468
Fragments arranged by size:
2468 2179 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3248 1937
Size: 1311 4594
Acc65I G'GTAC_C
Cuts at: 3226 3226
Size: 5905
AclI AA'CG_TT
Cuts at: 1563 4032 4405 1563
Size: 2469 373 3063
Fragments arranged by size:
3063 2469 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2089 2794 2962 3046 3968 5352 5620 1632
Size: 457 705 168 84 922 1384 268 1917
Fragments arranged by size:
1917 1384 922 705 457 268 168 84
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5708
AflIII A'CryG_T
Cuts at: 1203 4804 5781 1203
Size: 3601 977 1327
Fragments arranged by size:
3601 1327 977
AgeI A'CCGG_T
Cuts at: 4736 4736
Size: 5905
AhdI GACnn_n'nnGTC
Cuts at: 4634 4634
Size: 5905
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5905
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5905
AlwNI CAG_nnn'CTG
Cuts at: 2712 3648 5220 2712
Size: 936 1572 3397
Fragments arranged by size:
3397 1572 936
ApaLI G'TGCA_C
Cuts at: 3962 5118 3962
Size: 1156 4749
ApoI r'AATT_y
Cuts at: 1493 2030 2268 2555 3214 3440 3592 5701 1493
Size: 537 238 287 659 226 152 2109 1697
Fragments arranged by size:
2109 1697 659 537 287 238 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4459 160
Size: 872 3427 1606
Fragments arranged by size:
3427 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5905
AvaI C'yCGr_G
Cuts at: 1870 1943 3230 1870
Size: 73 1287 4545
Fragments arranged by size:
4545 1287 73
BamHI G'GATC_C
Cuts at: 3235 3602 3235
Size: 367 5538
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2664 3226 4681 618
Size: 325 217 627 877 562 1455 1842
Fragments arranged by size:
1842 1455 877 627 562 325 217
BanII G_rGCy'C
Cuts at: 1391 2181 2215 2990 3224 1391
Size: 790 34 775 234 4072
Fragments arranged by size:
4072 790 775 234 34
BbeI G_GCGC'C
Cuts at: 2668 2668
Size: 5905
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4934
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4096 4130 1903
Size: 34 2159 34 3678
Fragments arranged by size:
3678 2159 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2146 3797 5007 2146
Size: 1651 1210 3044
Fragments arranged by size:
3044 1651 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5905
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4516 136
Size: 107 122 71 631 605 2844 1525
Fragments arranged by size:
2844 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5900 5900
Size: 5905
BlpI GC'TnA_GC
Cuts at: 3322 3322
Size: 5905
Bme1580I G_kGCm'C
Cuts at: 1790 3966 5122 1790
Size: 2176 1156 2573
Fragments arranged by size:
2573 2176 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5905
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3636 4594 442
Size: 416 53 83 399 24 2219 958 1753
Fragments arranged by size:
2219 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5905
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2841 3093 2841
Size: 252 5653
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2117 2706 2790 2874 2958 3042 3126 4565
Size: 878 589 84 84 84 84 84 1439
Cuts at: 4565 1239
Size: 2579
Fragments arranged by size:
2579 1439 878 589 84 84 84 84
84
Bpu10I CC'TnA_GC
Cuts at: 2444 2482 2444
Size: 38 5867
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4030 4895 5193 4030
Size: 865 298 4742
Fragments arranged by size:
4742 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4568 882
Size: 617 3069 2219
Fragments arranged by size:
3069 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3696 493
Size: 711 2492 2702
Fragments arranged by size:
2702 2492 711
BsaBI GATnn'nnATC
Cuts at: 5899 5899
Size: 5905
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2665 3712 4094 4729
Size: 53 83 186 936 1132 1047 382 635
Cuts at: 4729 275
Size: 1451
Fragments arranged by size:
1451 1132 1047 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4338 4736 5010 5157 5568 4338
Size: 398 274 147 411 4675
Fragments arranged by size:
4675 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5680
Fragments arranged by size:
5680 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5905
BseYI C'CCAG_C
Cuts at: 5108 5604 5108
Size: 496 5409
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2819 1163
Size: 573 1083 4249
Fragments arranged by size:
4249 1083 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3355 4116 5144 1540
Size: 275 186 1354 761 1028 2301
Fragments arranged by size:
2301 1354 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2990 3966 4051 5122 2990
Size: 976 85 1071 3773
Fragments arranged by size:
3773 1071 976 85
BsmI GAATG_Cn'
Cuts at: 2517 2708 2792 2960 3416 3509 2517
Size: 191 84 168 456 93 4913
Fragments arranged by size:
4913 456 191 168 93 84
BsmBI CGTCTCn'nnnn_
Cuts at: 2829 2829
Size: 5905
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2181 2215 2990 3224 3966 4051 5122
Size: 399 391 34 775 234 742 85 1071
Cuts at: 5122 1391
Size: 2174
Fragments arranged by size:
2174 1071 775 742 399 391 234 85
34
BspEI T'CCGG_A
Cuts at: 5568 5568
Size: 5905
BspHI T'CATG_A
Cuts at: 3794 5524 3794
Size: 1730 4175
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3260 844
Size: 2416 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3792 1394
Size: 429 1969 3507
Fragments arranged by size:
3507 1969 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4400 4574 65
Size: 1183 3152 174 1396
Fragments arranged by size:
3152 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4549 4736 1700
Size: 110 2739 187 2869
Fragments arranged by size:
2869 2739 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5905
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5905
BssSI C'ACGA_G
Cuts at: 3965 4977 3965
Size: 1012 4893
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5905
BstBI TT'CG_AA
Cuts at: 4709 4709
Size: 5905
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5905
BstYI r'GATC_y
Cuts at: 1972 2534 3235 3602 3987 4004 5456 5542 5554
Size: 562 701 367 385 17 1452 86 12
Cuts at: 5554 5900 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 701 562 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5353
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2689 487
Size: 683 56 1463 3703
Fragments arranged by size:
3703 1463 683 56
BtsI GCAGTG_nn'
Cuts at: 2713 2797 3421 4215 4235 2713
Size: 84 624 794 20 4383
Fragments arranged by size:
4383 794 624 84 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5905
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5870
DraI TTT'AAA
Cuts at: 3209 3209
Size: 5905
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4912 809
Size: 507 3596 1802
Fragments arranged by size:
3596 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2115 2365 3352
Size: 54 1425 50 275 18 285 250 987
Cuts at: 3352 4241 5870 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 987 889 285 275 250 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3352 1537
Size: 275 1540 4090
Fragments arranged by size:
4090 1540 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2222 2713 3835 1353
Size: 869 491 1122 3423
Fragments arranged by size:
3423 1122 869 491
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4492 4866 5012 387
Size: 1064 138 2903 374 146 1280
Fragments arranged by size:
2903 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2988 2988
Size: 5905
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2089 2117 2706 2790 2794 2874 2958
Size: 393 457 28 589 84 4 80 84
Cuts at: 2958 2962 3042 3046 3126 3968 4565 5352 5620
Size: 4 80 4 80 842 597 787 268
Cuts at: 5620 1239
Size: 1524
Fragments arranged by size:
1524 842 787 597 589 457 393 268
84 84 80 80 80 28 4 4
4
EcoO109I rG'GnC_Cy
Cuts at: 2074 3141 2074
Size: 1067 4838
EcoRI G'AATT_C
Cuts at: 2030 2555 3214 3592 2030
Size: 525 659 378 4343
Fragments arranged by size:
4343 659 525 378
FspI TGC'GCA
Cuts at: 4411 4411
Size: 5905
HaeII r_GCGC'y
Cuts at: 1678 2668 5052 1678
Size: 990 2384 2531
Fragments arranged by size:
2531 2384 990
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4546 4578 4620 4652 1373
Size: 32 135 32 2974 32 42 32 2626
Fragments arranged by size:
2974 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2418 2506 3249 3495 669
Size: 988 281 480 88 743 246 3079
Fragments arranged by size:
3079 988 743 480 281 246 88
HindIII A'AGCT_T
Cuts at: 748 2479 748
Size: 1731 4174
HpaI GTT'AAC
Cuts at: 2506 3495 2506
Size: 989 4916
KasI G'GCGC_C
Cuts at: 2664 2664
Size: 5905
KpnI G_GTAC'C
Cuts at: 3230 3230
Size: 5905
MfeI C'AATT_G
Cuts at: 3504 3504
Size: 5905
MluI A'CGCG_T
Cuts at: 5781 5781
Size: 5905
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2142 5019 5203 1305
Size: 837 2877 184 2007
Fragments arranged by size:
2877 2007 837 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2117 2367 10
Size: 54 1425 343 285 250 3548
Fragments arranged by size:
3548 1425 343 285 250 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2522 3863 4222 4381 518
Size: 648 1356 1341 359 159 2042
Fragments arranged by size:
2042 1356 1341 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2056 3321 3998 5146 5391 1606
Size: 450 1265 677 1148 245 2120
Fragments arranged by size:
2120 1265 1148 677 450 245
NarI GG'CG_CC
Cuts at: 2665 2665
Size: 5905
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5353
NdeI CA'TA_TG
Cuts at: 387 2146 387
Size: 1759 4146
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5905
NotI GC'GGCC_GC
Cuts at: 3352 3352
Size: 5905
NspI r_CATG'y
Cuts at: 1132 1229 3263 4808 1132
Size: 97 2034 1545 2229
Fragments arranged by size:
2229 2034 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5905
PciI A'CATG_T
Cuts at: 4804 4804
Size: 5905
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5905
PmeI GTTT'AAAC
Cuts at: 3209 3209
Size: 5905
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3974 4006 5487 5519 3974
Size: 32 1481 32 4360
Fragments arranged by size:
4360 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2074 2074
Size: 5905
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5905
PsiI TTA'TAA
Cuts at: 2457 2541 3475 2457
Size: 84 934 4887
Fragments arranged by size:
4887 934 84
PstI C_TGCA'G
Cuts at: 830 2331 3257 830
Size: 1501 926 3478
Fragments arranged by size:
3478 1501 926
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5905
PvuII CAG'CTG
Cuts at: 2056 2056
Size: 5905
SacI G_AGCT'C
Cuts at: 2990 2990
Size: 5905
SalI G'TCGA_C
Cuts at: 1936 3247 1936
Size: 1311 4594
SapI GCTCTTCn'nnn_
Cuts at: 2713 2713
Size: 5905
SbfI CC_TGCA'GG
Cuts at: 3257 3257
Size: 5905
ScaI AGT'ACT
Cuts at: 4153 5865 4153
Size: 1712 4193
SfcI C'TryA_G
Cuts at: 826 1045 2080 2327 2839 3253 4388 5069 5260
Size: 219 1035 247 512 414 1135 681 191
Cuts at: 5260 826
Size: 1471
Fragments arranged by size:
1471 1135 1035 681 512 414 247 219
191
SfoI GGC'GCC
Cuts at: 2666 2666
Size: 5905
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5905
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5905
SmaI CCC'GGG
Cuts at: 1872 3232 1872
Size: 1360 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4009 4910 5172 820
Size: 197 926 2066 901 262 1553
Fragments arranged by size:
2066 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3696 493
Size: 3203 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5905
SphI G_CATG'C
Cuts at: 1132 3263 1132
Size: 2131 3774
SspI AAT'ATT
Cuts at: 5 52 3829 5
Size: 47 3777 2081
Fragments arranged by size:
3777 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5905
StyI C'CwwG_G
Cuts at: 513 1065 2244 3344 513
Size: 552 1179 1100 3074
Fragments arranged by size:
3074 1179 1100 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2613 3932 3949 4102 4287 4732 325
Size: 1255 1033 1319 17 153 185 445 1498
Fragments arranged by size:
1498 1319 1255 1033 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4151 5863 96
Size: 274 80 33 51 1443 2174 1712 138
Fragments arranged by size:
2174 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2891 2923 5865 5897 2891
Size: 32 2942 32 2899
Fragments arranged by size:
2942 2899 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5905
XbaI T'CTAG_A
Cuts at: 3241 3241
Size: 5905
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5905
XmaI C'CCGG_G
Cuts at: 1870 3230 1870
Size: 1360 4545
XmnI GAAnn'nnTTC
Cuts at: 4034 4034
Size: 5905
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3713 276
Size: 53 83 186 936 2179 2468
Fragments arranged by size:
2468 2179 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI
SexAI SfiI SrfI SwaI XcmI