Restriction map for
pFN21AB8734
(Circular) (Six-base) MAPSORT of: pFN21AB8734.seq Check: 5478 from: 1 to: 6358
pFN21AB8734 6358 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2017 2017
Size: 6358
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4168 278
Size: 53 83 186 936 2632 2468
Fragments arranged by size:
2632 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3701 1937
Size: 1764 4594
Acc65I G'GTAC_C
Cuts at: 3679 3679
Size: 6358
AclI AA'CG_TT
Cuts at: 1563 3005 4485 4858 1563
Size: 1442 1480 373 3063
Fragments arranged by size:
3063 1480 1442 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2432 2844 4421 5805 6073 1632
Size: 800 412 1577 1384 268 1917
Fragments arranged by size:
1917 1577 1384 800 412 268
AflII C'TTAA_G
Cuts at: 820 1017 3292 820
Size: 197 2275 3886
Fragments arranged by size:
3886 2275 197
AflIII A'CryG_T
Cuts at: 1203 5257 6234 1203
Size: 4054 977 1327
Fragments arranged by size:
4054 1327 977
AgeI A'CCGG_T
Cuts at: 5189 5189
Size: 6358
AhdI GACnn_n'nnGTC
Cuts at: 5087 5087
Size: 6358
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6358
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6358
AlwNI CAG_nnn'CTG
Cuts at: 2822 4101 5673 2822
Size: 1279 1572 3507
Fragments arranged by size:
3507 1572 1279
ApaLI G'TGCA_C
Cuts at: 4415 5571 4415
Size: 1156 5202
ApoI r'AATT_y
Cuts at: 1493 3667 3893 4045 6154 1493
Size: 2174 226 152 2109 1697
Fragments arranged by size:
2174 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2549 4912 160
Size: 872 1517 2363 1606
Fragments arranged by size:
2363 1606 1517 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6358
AvaI C'yCGr_G
Cuts at: 1870 1943 3683 1870
Size: 73 1740 4545
Fragments arranged by size:
4545 1740 73
AvrII C'CTAG_G
Cuts at: 2183 2288 2183
Size: 105 6253
BamHI G'GATC_C
Cuts at: 3031 3688 4055 3031
Size: 657 367 5334
Fragments arranged by size:
5334 657 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2046 3679 5134 618
Size: 325 217 627 259 1633 1455 1842
Fragments arranged by size:
1842 1633 1455 627 325 259 217
BanII G_rGCy'C
Cuts at: 1391 3677 1391
Size: 2286 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2152 928
Size: 971 253 5134
Fragments arranged by size:
5134 971 253
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4549 4583 1903
Size: 34 2612 34 3678
Fragments arranged by size:
3678 2612 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4250 5460 4250
Size: 1210 5148
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6358
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4969 136
Size: 107 122 71 631 605 3297 1525
Fragments arranged by size:
3297 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3178 6353 3178
Size: 3175 3183
BlpI GC'TnA_GC
Cuts at: 3775 3775
Size: 6358
Bme1580I G_kGCm'C
Cuts at: 1790 2049 4419 5575 1790
Size: 259 2370 1156 2573
Fragments arranged by size:
2573 2370 1156 259
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6358
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3084 4089 5047
Size: 416 53 83 399 24 1667 1005 958
Cuts at: 5047 442
Size: 1753
Fragments arranged by size:
1753 1667 1005 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2013 1056
Size: 957 5401
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2057 2569 3088 3094 3100 3106 5018 1239
Size: 818 512 519 6 6 6 1912 2579
Fragments arranged by size:
2579 1912 818 519 512 6 6 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4483 5348 5646 4483
Size: 865 298 5195
Fragments arranged by size:
5195 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5021 882
Size: 617 3522 2219
Fragments arranged by size:
3522 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4149 493
Size: 711 2945 2702
Fragments arranged by size:
2945 2702 711
BsaBI GATnn'nnATC
Cuts at: 3588 6352 3588
Size: 2764 3594
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4165 4547 5182 275
Size: 53 83 186 936 2632 382 635 1451
Fragments arranged by size:
2632 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2119 4791 5189 5463 5610 6021 2119
Size: 2672 398 274 147 411 2456
Fragments arranged by size:
2672 2456 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6133
Fragments arranged by size:
6133 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3068 3215 3279 1186
Size: 1882 147 64 4265
Fragments arranged by size:
4265 1882 147 64
BseYI C'CCAG_C
Cuts at: 2041 5561 6057 2041
Size: 3520 496 2342
Fragments arranged by size:
3520 2342 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2045 1163
Size: 573 309 5476
Fragments arranged by size:
5476 573 309
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3808 4569 5597 1540
Size: 275 186 1807 761 1028 2301
Fragments arranged by size:
2301 1807 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2127 2562 4419 4504 5575 2127
Size: 435 1857 85 1071 2910
Fragments arranged by size:
2910 1857 1071 435 85
BsiWI C'GTAC_G
Cuts at: 2110 2110
Size: 6358
BsmI GAATG_Cn'
Cuts at: 3184 3869 3962 3184
Size: 685 93 5580
Fragments arranged by size:
5580 685 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2049 2127 2562 3677 4419 4504 5575
Size: 399 259 78 435 1115 742 85 1071
Cuts at: 5575 1391
Size: 2174
Fragments arranged by size:
2174 1115 1071 742 435 399 259 85
78
BspEI T'CCGG_A
Cuts at: 2119 6021 2119
Size: 3902 2456
BspHI T'CATG_A
Cuts at: 4247 5977 4247
Size: 1730 4628
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2017 3713 844
Size: 1173 1696 3489
Fragments arranged by size:
3489 1696 1173
BsrBI CCG'CTC
Cuts at: 1394 1823 4245 1394
Size: 429 2422 3507
Fragments arranged by size:
3507 2422 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4853 5027 65
Size: 1183 3605 174 1396
Fragments arranged by size:
3605 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5002 5189 1700
Size: 110 3192 187 2869
Fragments arranged by size:
3192 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6358
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6358
BssSI C'ACGA_G
Cuts at: 2014 4418 5430 2014
Size: 2404 1012 2942
Fragments arranged by size:
2942 2404 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6358
BstBI TT'CG_AA
Cuts at: 5162 5162
Size: 6358
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6358
BstYI r'GATC_y
Cuts at: 1972 2206 3031 3178 3688 4055 4440 4457 5909
Size: 234 825 147 510 367 385 17 1452
Cuts at: 5909 5995 6007 6353 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 825 510 385 367 346 234
147 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2076 513
Size: 552 939 72 4795
Fragments arranged by size:
4795 939 552 72
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5619
Fragments arranged by size:
5619 683 56
BtsI GCAGTG_nn'
Cuts at: 3874 4668 4688 3874
Size: 794 20 5544
Fragments arranged by size:
5544 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6358
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6323
DraI TTT'AAA
Cuts at: 2272 2875 3662 2272
Size: 603 787 4968
Fragments arranged by size:
4968 787 603
DraIII CAC_nnn'GTG
Cuts at: 2146 2146
Size: 6358
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5365 809
Size: 507 4049 1802
Fragments arranged by size:
4049 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3805 4694 6323
Size: 54 1425 50 275 18 1975 889 1629
Cuts at: 6323 8
Size: 43
Fragments arranged by size:
1975 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3805 1537
Size: 275 1993 4090
Fragments arranged by size:
4090 1993 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2679 4288 1353
Size: 1326 1609 3423
Fragments arranged by size:
3423 1609 1326
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4945 5319 5465 387
Size: 1064 138 3356 374 146 1280
Fragments arranged by size:
3356 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2057 2432 2569 2844 3088 3094 3100
Size: 393 425 375 137 275 244 6 6
Cuts at: 3100 3106 4421 5018 5805 6073 1239
Size: 6 1315 597 787 268 1524
Fragments arranged by size:
1524 1315 787 597 425 393 375 275
268 244 137 6 6 6
EcoO109I rG'GnC_Cy
Cuts at: 2425 3155 2425
Size: 730 5628
EcoRI G'AATT_C
Cuts at: 3667 4045 3667
Size: 378 5980
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2725 2725
Size: 6358
FspI TGC'GCA
Cuts at: 4864 4864
Size: 6358
HaeII r_GCGC'y
Cuts at: 1678 5505 1678
Size: 3827 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3047 3079 3191 3221 3223
Size: 32 135 32 1475 32 112 30 2
Cuts at: 3223 3253 3581 3613 4999 5031 5073 5105 1373
Size: 30 328 32 1386 32 42 32 2626
Fragments arranged by size:
2626 1475 1386 328 135 112 42 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 669 1657 1938 3702 3948 669
Size: 988 281 1764 246 3079
Fragments arranged by size:
3079 1764 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2176 748
Size: 1428 4930
HpaI GTT'AAC
Cuts at: 3948 3948
Size: 6358
KpnI G_GTAC'C
Cuts at: 3683 3683
Size: 6358
MfeI C'AATT_G
Cuts at: 2256 3957 2256
Size: 1701 4657
MluI A'CGCG_T
Cuts at: 6234 6234
Size: 6358
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5472 5656 1305
Size: 4167 184 2007
Fragments arranged by size:
4167 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4536
Fragments arranged by size:
4536 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2598 3189 4316 4675 4834 518
Size: 648 1432 591 1127 359 159 2042
Fragments arranged by size:
2042 1432 1127 648 591 359 159
MspA1I CmG'CkG
Cuts at: 1606 2358 3774 4451 5599 5844 1606
Size: 752 1416 677 1148 245 2120
Fragments arranged by size:
2120 1416 1148 752 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4867
Fragments arranged by size:
4867 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6358
NheI G'CTAG_C
Cuts at: 1052 2009 1052
Size: 957 5401
NotI GC'GGCC_GC
Cuts at: 3805 3805
Size: 6358
NspI r_CATG'y
Cuts at: 1132 1229 3716 5261 1132
Size: 97 2487 1545 2229
Fragments arranged by size:
2487 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6358
PciI A'CATG_T
Cuts at: 5257 5257
Size: 6358
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6358
PmeI GTTT'AAAC
Cuts at: 3662 3662
Size: 6358
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4427 4459 5940 5972 4427
Size: 32 1481 32 4813
Fragments arranged by size:
4813 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2425 3155 2425
Size: 730 5628
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6358
PsiI TTA'TAA
Cuts at: 3928 3928
Size: 6358
PstI C_TGCA'G
Cuts at: 830 3525 3710 830
Size: 2695 185 3478
Fragments arranged by size:
3478 2695 185
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6358
RsrII CG'GwC_CG
Cuts at: 2117 2117
Size: 6358
SalI G'TCGA_C
Cuts at: 1936 3700 1936
Size: 1764 4594
SanDI GG'GwC_CC
Cuts at: 2425 2425
Size: 6358
SbfI CC_TGCA'GG
Cuts at: 3710 3710
Size: 6358
ScaI AGT'ACT
Cuts at: 4606 6318 4606
Size: 1712 4646
SfcI C'TryA_G
Cuts at: 826 1045 3521 3626 3706 4841 5522 5713 826
Size: 219 2476 105 80 1135 681 191 1471
Fragments arranged by size:
2476 1471 1135 681 219 191 105 80
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6358
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6358
SmaI CCC'GGG
Cuts at: 1872 3685 1872
Size: 1813 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3292 4462 5363 5625 820
Size: 197 926 1349 1170 901 262 1553
Fragments arranged by size:
1553 1349 1170 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4149 493
Size: 3656 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6358
SphI G_CATG'C
Cuts at: 1132 3716 1132
Size: 2584 3774
SspI AAT'ATT
Cuts at: 5 52 4282 5
Size: 47 4230 2081
Fragments arranged by size:
4230 2081 47
StuI AGG'CCT
Cuts at: 1517 3253 1517
Size: 1736 4622
StyI C'CwwG_G
Cuts at: 513 1065 2004 2183 2288 3797 513
Size: 552 939 179 105 1509 3074
Fragments arranged by size:
3074 1509 939 552 179 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2105 4385 4402 4555 4740 5185 325
Size: 1255 525 2280 17 153 185 445 1498
Fragments arranged by size:
2280 1498 1255 525 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4604 6316 96
Size: 274 80 33 51 1443 2627 1712 138
Fragments arranged by size:
2627 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6318 6350 6318
Size: 32 6326
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6358
XbaI T'CTAG_A
Cuts at: 3694 3694
Size: 6358
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6358
XmaI C'CCGG_G
Cuts at: 1870 3683 1870
Size: 1813 4545
XmnI GAAnn'nnTTC
Cuts at: 2178 4487 2178
Size: 2309 4049
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4166 276
Size: 53 83 186 936 2632 2468
Fragments arranged by size:
2632 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI RsrII SalI SanDI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII
SacI SacII SapI SexAI SfiI SfoI SrfI SwaI
XcmI