Restriction map for
pFN21AB8732
(Circular) (Six-base) MAPSORT of: pFN21AB8732.seq Check: 8170 from: 1 to: 6433
pFN21AB8732 6433 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3480 3480
Size: 6433
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4243 278
Size: 53 83 186 936 2707 2468
Fragments arranged by size:
2707 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3050 3776 1937
Size: 1113 726 4594
Fragments arranged by size:
4594 1113 726
Acc65I G'GTAC_C
Cuts at: 3754 3754
Size: 6433
AclI AA'CG_TT
Cuts at: 1563 4560 4933 1563
Size: 2997 373 3063
Fragments arranged by size:
3063 2997 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2972 4496 5880 6148 1632
Size: 1340 1524 1384 268 1917
Fragments arranged by size:
1917 1524 1384 1340 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6236
AflIII A'CryG_T
Cuts at: 1203 5332 6309 1203
Size: 4129 977 1327
Fragments arranged by size:
4129 1327 977
AgeI A'CCGG_T
Cuts at: 5264 5264
Size: 6433
AhdI GACnn_n'nnGTC
Cuts at: 5162 5162
Size: 6433
AleI CACnn'nnGTG
Cuts at: 1166 2429 3296 1166
Size: 1263 867 4303
Fragments arranged by size:
4303 1263 867
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2420 1184
Size: 1236 5197
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2000 2032 2000
Size: 32 6401
AlwNI CAG_nnn'CTG
Cuts at: 2068 2234 2714 2933 3043 3472 3520 3532 3559
Size: 166 480 219 110 429 48 12 27
Cuts at: 3559 4176 5748 2068
Size: 617 1572 2753
Fragments arranged by size:
2753 1572 617 480 429 219 166 110
48 27 12
ApaLI G'TGCA_C
Cuts at: 4490 5646 4490
Size: 1156 5277
ApoI r'AATT_y
Cuts at: 1493 2192 2199 2306 2569 3742 3968 4120 6229
Size: 699 7 107 263 1173 226 152 2109
Cuts at: 6229 1493
Size: 1697
Fragments arranged by size:
2109 1697 1173 699 263 226 152 107
7
AseI AT'TA_AT
Cuts at: 160 1032 3507 4987 160
Size: 872 2475 1480 1606
Fragments arranged by size:
2475 1606 1480 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6433
AvaI C'yCGr_G
Cuts at: 1870 1943 3758 1870
Size: 73 1815 4545
Fragments arranged by size:
4545 1815 73
AvrII C'CTAG_G
Cuts at: 2239 3143 2239
Size: 904 5529
BamHI G'GATC_C
Cuts at: 2449 3763 4130 2449
Size: 1314 367 4752
Fragments arranged by size:
4752 1314 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2579 3754 5209 618
Size: 325 217 627 792 1175 1455 1842
Fragments arranged by size:
1842 1455 1175 792 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3244 3653 3752 1391
Size: 1853 409 99 4072
Fragments arranged by size:
4072 1853 409 99
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5462
BbvCI CC'TCA_GC
Cuts at: 2124 2276 2124
Size: 152 6281
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4624 4658 1903
Size: 34 2687 34 3678
Fragments arranged by size:
3678 2687 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4325 5535 4325
Size: 1210 5223
BclI T'GATC_A
Cuts at: 1548 2460 1548
Size: 912 5521
BfrBI ATG'CAT
Cuts at: 3412 3412
Size: 6433
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2963 5044 136
Size: 107 122 71 631 605 1291 2081 1525
Fragments arranged by size:
2081 1525 1291 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6428 6428
Size: 6433
BlpI GC'TnA_GC
Cuts at: 2549 3280 3850 2549
Size: 731 570 5132
Fragments arranged by size:
5132 731 570
Bme1580I G_kGCm'C
Cuts at: 1790 4494 5650 1790
Size: 2704 1156 2573
Fragments arranged by size:
2704 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6433
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2311 2431 2551
Size: 416 53 83 399 24 894 120 120
Cuts at: 2551 4164 5122 442
Size: 1613 958 1753
Fragments arranged by size:
1753 1613 958 894 416 399 120 120
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6433
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2205 2595 2691 3113 3380 3479 3530 5093
Size: 966 390 96 422 267 99 51 1563
Cuts at: 5093 1239
Size: 2579
Fragments arranged by size:
2579 1563 966 422 390 267 99 96
51
Bpu10I CC'TnA_GC
Cuts at: 2124 2276 2124
Size: 152 6281
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2344 3690 4558 5423 5721 2344
Size: 1346 868 865 298 3056
Fragments arranged by size:
3056 1346 868 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2863 5096 882
Size: 617 1364 2233 2219
Fragments arranged by size:
2233 2219 1364 617
BsaAI yAC'GTr
Cuts at: 493 1204 4224 493
Size: 711 3020 2702
Fragments arranged by size:
3020 2702 711
BsaBI GATnn'nnATC
Cuts at: 6427 6427
Size: 6433
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3047 4240 4622 5257
Size: 53 83 186 936 1514 1193 382 635
Cuts at: 5257 275
Size: 1451
Fragments arranged by size:
1514 1451 1193 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4866 5264 5538 5685 6096 4866
Size: 398 274 147 411 5203
Fragments arranged by size:
5203 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2203 2233 1178
Size: 30 165 30 800 30 5378
Fragments arranged by size:
5378 800 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2208 3582 1186
Size: 1022 1374 4037
Fragments arranged by size:
4037 1374 1022
BseYI C'CCAG_C
Cuts at: 2520 3318 3654 5636 6132 2520
Size: 798 336 1982 496 2821
Fragments arranged by size:
2821 1982 798 496 336
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5860
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2034 3883 4644 5672 1540
Size: 275 186 33 1849 761 1028 2301
Fragments arranged by size:
2301 1849 1028 761 275 186 33
BsiHKAI G_wGCw'C
Cuts at: 4494 4579 5650 4494
Size: 85 1071 5277
Fragments arranged by size:
5277 1071 85
BsmI GAATG_Cn'
Cuts at: 3944 4037 3944
Size: 93 6340
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3244 3653 3752 4494 4579 5650 1391
Size: 399 1454 409 99 742 85 1071 2174
Fragments arranged by size:
2174 1454 1071 742 409 399 99 85
BspEI T'CCGG_A
Cuts at: 6096 6096
Size: 6433
BspHI T'CATG_A
Cuts at: 4322 6052 4322
Size: 1730 4703
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2899 3480 3709 3788 844
Size: 2055 581 229 79 3489
Fragments arranged by size:
3489 2055 581 229 79
BsrBI CCG'CTC
Cuts at: 1394 1823 4320 1394
Size: 429 2497 3507
Fragments arranged by size:
3507 2497 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2285 3210 4928 5102 65
Size: 1183 1037 925 1718 174 1396
Fragments arranged by size:
1718 1396 1183 1037 925 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2442 5077 5264 1700
Size: 110 632 2635 187 2869
Fragments arranged by size:
2869 2635 632 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6433
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6433
BssSI C'ACGA_G
Cuts at: 2369 4493 5505 2369
Size: 2124 1012 3297
Fragments arranged by size:
3297 2124 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3331 3379 3409 3433 3460 3472 3532 3559
Size: 2537 48 30 24 27 12 60 27
Cuts at: 3559 3568 794
Size: 9 3659
Fragments arranged by size:
3659 2537 60 48 30 27 27 24
12 9
BstBI TT'CG_AA
Cuts at: 2196 5237 2196
Size: 3041 3392
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6433
BstYI r'GATC_y
Cuts at: 1972 2449 2755 3763 4130 4515 4532 5984 6070
Size: 477 306 1008 367 385 17 1452 86
Cuts at: 6070 6082 6428 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1008 477 385 367 346 306
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2025 3258 3270 513
Size: 552 939 21 1233 12 3676
Fragments arranged by size:
3676 1233 939 552 21 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5694
Fragments arranged by size:
5694 683 56
BtsI GCAGTG_nn'
Cuts at: 2069 2404 3949 4743 4763 2069
Size: 335 1545 794 20 3739
Fragments arranged by size:
3739 1545 794 335 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6433
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6398
DraI TTT'AAA
Cuts at: 3737 3737
Size: 6433
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3046 5440 809
Size: 507 1730 2394 1802
Fragments arranged by size:
2394 1802 1730 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2031 3880 4769
Size: 54 1425 50 275 18 201 1849 889
Cuts at: 4769 6398 8
Size: 1629 43
Fragments arranged by size:
1849 1629 1425 889 275 201 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2031 3880 1537
Size: 275 219 1849 4090
Fragments arranged by size:
4090 1849 275 219
EarI CTCTTCn'nnn_
Cuts at: 1353 2652 4363 1353
Size: 1299 1711 3423
Fragments arranged by size:
3423 1711 1299
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5020 5394 5540 387
Size: 1064 138 3431 374 146 1280
Fragments arranged by size:
3431 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2205 2595 2691 2972 3113 3380 3479
Size: 393 573 390 96 281 141 267 99
Cuts at: 3479 3530 4496 5093 5880 6148 1239
Size: 51 966 597 787 268 1524
Fragments arranged by size:
1524 966 787 597 573 393 390 281
268 267 141 99 96 51
EcoNI CCTnn'n_nnAGG
Cuts at: 2238 2238
Size: 6433
EcoO109I rG'GnC_Cy
Cuts at: 2038 2956 2038
Size: 918 5515
EcoRI G'AATT_C
Cuts at: 2192 2306 3742 4120 2192
Size: 114 1436 378 4505
Fragments arranged by size:
4505 1436 378 114
FspI TGC'GCA
Cuts at: 4939 4939
Size: 6433
HaeII r_GCGC'y
Cuts at: 1678 2556 3544 5580 1678
Size: 878 988 2036 2531
Fragments arranged by size:
2531 2036 988 878
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3688 3720 5074 5106 5148
Size: 32 135 32 2116 32 1354 32 42
Cuts at: 5148 5180 1373
Size: 32 2626
Fragments arranged by size:
2626 2116 1354 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3051 3777 4023 669
Size: 988 281 1113 726 246 3079
Fragments arranged by size:
3079 1113 988 726 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6433
HpaI GTT'AAC
Cuts at: 4023 4023
Size: 6433
KpnI G_GTAC'C
Cuts at: 3758 3758
Size: 6433
MfeI C'AATT_G
Cuts at: 3335 4032 3335
Size: 697 5736
MluI A'CGCG_T
Cuts at: 6309 6309
Size: 6433
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5547 5731 1305
Size: 4242 184 2007
Fragments arranged by size:
4242 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4611
Fragments arranged by size:
4611 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2397 2429 2878 3296 4391 4750
Size: 648 843 388 32 449 418 1095 359
Cuts at: 4750 4909 518
Size: 159 2042
Fragments arranged by size:
2042 1095 843 648 449 418 388 359
159 32
MspA1I CmG'CkG
Cuts at: 1606 2027 3520 3532 3550 3559 3849 4526 5674
Size: 421 1493 12 18 9 290 677 1148
Cuts at: 5674 5919 1606
Size: 245 2120
Fragments arranged by size:
2120 1493 1148 677 421 290 245 18
12 9
NcoI C'CATG_G
Cuts at: 513 1065 2004 3258 3270 513
Size: 552 939 1254 12 3676
Fragments arranged by size:
3676 1254 939 552 12
NdeI CA'TA_TG
Cuts at: 387 2409 2788 387
Size: 2022 379 4032
Fragments arranged by size:
4032 2022 379
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6433
NotI GC'GGCC_GC
Cuts at: 3880 3880
Size: 6433
NsiI A_TGCA'T
Cuts at: 3414 3414
Size: 6433
NspI r_CATG'y
Cuts at: 1132 1229 2248 3226 3463 3571 3791 5336 1132
Size: 97 1019 978 237 108 220 1545 2229
Fragments arranged by size:
2229 1545 1019 978 237 220 108 97
PasI CC'CwG_GG
Cuts at: 1348 2296 1348
Size: 948 5485
PciI A'CATG_T
Cuts at: 5332 5332
Size: 6433
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2180 2714 1108
Size: 1072 534 4827
Fragments arranged by size:
4827 1072 534
PmeI GTTT'AAAC
Cuts at: 3737 3737
Size: 6433
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4502 4534 6015 6047 4502
Size: 32 1481 32 4888
Fragments arranged by size:
4888 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2038 2038
Size: 6433
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6433
PsiI TTA'TAA
Cuts at: 4003 4003
Size: 6433
PstI C_TGCA'G
Cuts at: 830 3477 3525 3537 3555 3785 830
Size: 2647 48 12 18 230 3478
Fragments arranged by size:
3478 2647 230 48 18 12
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6433
PvuII CAG'CTG
Cuts at: 3520 3532 3550 3559 3520
Size: 12 18 9 6394
Fragments arranged by size:
6394 18 12 9
SacII CC_GC'GG
Cuts at: 2028 2028
Size: 6433
SalI G'TCGA_C
Cuts at: 1936 3049 3775 1936
Size: 1113 726 4594
Fragments arranged by size:
4594 1113 726
SanDI GG'GwC_CC
Cuts at: 2038 2038
Size: 6433
SbfI CC_TGCA'GG
Cuts at: 3785 3785
Size: 6433
ScaI AGT'ACT
Cuts at: 4681 6393 4681
Size: 1712 4721
SfcI C'TryA_G
Cuts at: 826 1045 2083 3473 3521 3533 3551 3781 4916
Size: 219 1038 1390 48 12 18 230 1135
Cuts at: 4916 5597 5788 826
Size: 681 191 1471
Fragments arranged by size:
1471 1390 1135 1038 681 230 219 191
48 18 12
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6433
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6433
SmaI CCC'GGG
Cuts at: 1872 3760 1872
Size: 1888 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2359 3705 4537 5438 5700 820
Size: 197 926 416 1346 832 901 262 1553
Fragments arranged by size:
1553 1346 926 901 832 416 262 197
SnaBI TAC'GTA
Cuts at: 493 4224 493
Size: 3731 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6433
SphI G_CATG'C
Cuts at: 1132 2248 3791 1132
Size: 1116 1543 3774
Fragments arranged by size:
3774 1543 1116
SspI AAT'ATT
Cuts at: 5 52 4357 5
Size: 47 4305 2081
Fragments arranged by size:
4305 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6433
StyI C'CwwG_G
Cuts at: 513 1065 2004 2179 2239 2890 3143 3258 3270
Size: 552 939 175 60 651 253 115 12
Cuts at: 3270 3872 513
Size: 602 3074
Fragments arranged by size:
3074 939 651 602 552 253 175 115
60 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2501 3308 4460 4477 4630 4815 5260
Size: 1255 921 807 1152 17 153 185 445
Cuts at: 5260 325
Size: 1498
Fragments arranged by size:
1498 1255 1152 921 807 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2254 4679 6391
Size: 274 80 33 51 1443 277 2425 1712
Cuts at: 6391 96
Size: 138
Fragments arranged by size:
2425 1712 1443 277 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2203 2235 6393 6425 2203
Size: 32 4158 32 2211
Fragments arranged by size:
4158 2211 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6433
XbaI T'CTAG_A
Cuts at: 3769 3769
Size: 6433
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6433
XmaI C'CCGG_G
Cuts at: 1870 3758 1870
Size: 1888 4545
XmnI GAAnn'nnTTC
Cuts at: 4562 4562
Size: 6433
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4241 276
Size: 53 83 186 936 2707 2468
Fragments arranged by size:
2707 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI
SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI BaeI BbeI BplI BsiWI BsmBI
BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI
PmlI PspOMI PspXI PsrI RsrII SacI SapI SexAI
SfiI SfoI SrfI SwaI XcmI