Restriction map for
pFN21AB8730
(Circular) (Six-base) MAPSORT of: pFN21AB8730.seq Check: 2496 from: 1 to: 5884
pFN21AB8730 5884 bp
With 182 enzymes: SgfI *
May 6, 2010 17:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3694 278
Size: 53 83 186 936 2158 2468
Fragments arranged by size:
2468 2158 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3131 3227 1937
Size: 1194 96 4594
Fragments arranged by size:
4594 1194 96
Acc65I G'GTAC_C
Cuts at: 3205 3205
Size: 5884
AclI AA'CG_TT
Cuts at: 1563 2094 4011 4384 1563
Size: 531 1917 373 3063
Fragments arranged by size:
3063 1917 531 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2190 2978 3131 3947 5331 5599 1632
Size: 558 788 153 816 1384 268 1917
Fragments arranged by size:
1917 1384 816 788 558 268 153
AfeI AGC'GCT
Cuts at: 2546 3014 2546
Size: 468 5416
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5687
AflIII A'CryG_T
Cuts at: 1203 2712 4783 5760 1203
Size: 1509 2071 977 1327
Fragments arranged by size:
2071 1509 1327 977
AgeI A'CCGG_T
Cuts at: 4715 4715
Size: 5884
AhdI GACnn_n'nnGTC
Cuts at: 4613 4613
Size: 5884
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5884
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5884
AlwNI CAG_nnn'CTG
Cuts at: 2210 2727 3627 5199 2210
Size: 517 900 1572 2895
Fragments arranged by size:
2895 1572 900 517
ApaLI G'TGCA_C
Cuts at: 3941 5097 3941
Size: 1156 4728
ApoI r'AATT_y
Cuts at: 1493 2602 3193 3419 3571 5680 1493
Size: 1109 591 226 152 2109 1697
Fragments arranged by size:
2109 1697 1109 591 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4438 160
Size: 872 3406 1606
Fragments arranged by size:
3406 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5884
AvaI C'yCGr_G
Cuts at: 1870 1943 3209 1870
Size: 73 1266 4545
Fragments arranged by size:
4545 1266 73
BamHI G'GATC_C
Cuts at: 3214 3581 3214
Size: 367 5517
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2465 2656 3205 4660 618
Size: 325 217 627 678 191 549 1455 1842
Fragments arranged by size:
1842 1455 678 627 549 325 217 191
BanII G_rGCy'C
Cuts at: 1391 2040 3097 3203 1391
Size: 649 1057 106 4072
Fragments arranged by size:
4072 1057 649 106
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2178 2784 928
Size: 971 279 606 4028
Fragments arranged by size:
4028 971 606 279
BbvCI CC'TCA_GC
Cuts at: 2577 2577
Size: 5884
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4075 4109 1903
Size: 34 2138 34 3678
Fragments arranged by size:
3678 2138 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3776 4986 3776
Size: 1210 4674
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5884
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4495 136
Size: 107 122 71 631 605 2823 1525
Fragments arranged by size:
2823 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5879 5879
Size: 5884
BlpI GC'TnA_GC
Cuts at: 3301 3301
Size: 5884
Bme1580I G_kGCm'C
Cuts at: 1790 2661 3945 5101 1790
Size: 871 1284 1156 2573
Fragments arranged by size:
2573 1284 1156 871
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5884
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2807 3615 4573
Size: 416 53 83 399 24 1390 808 958
Cuts at: 4573 442
Size: 1753
Fragments arranged by size:
1753 1390 958 808 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5884
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2566 2698 3111 2566
Size: 132 413 5339
Fragments arranged by size:
5339 413 132
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2767 3085 4544 1239
Size: 1528 318 1459 2579
Fragments arranged by size:
2579 1528 1459 318
Bpu10I CC'TnA_GC
Cuts at: 2577 2577
Size: 5884
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3038 4009 4874 5172 3038
Size: 971 865 298 3750
Fragments arranged by size:
3750 971 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2838 4547 882
Size: 617 1339 1709 2219
Fragments arranged by size:
2219 1709 1339 617
BsaAI yAC'GTr
Cuts at: 493 1204 2379 3675 493
Size: 711 1175 1296 2702
Fragments arranged by size:
2702 1296 1175 711
BsaBI GATnn'nnATC
Cuts at: 5878 5878
Size: 5884
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3691 4073 4708 275
Size: 53 83 186 936 2158 382 635 1451
Fragments arranged by size:
2158 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2745 4317 4715 4989 5136 5547 2745
Size: 1572 398 274 147 411 3082
Fragments arranged by size:
3082 1572 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2003 2033 2927 2957 1178
Size: 30 165 30 600 30 894 30 4105
Fragments arranged by size:
4105 894 600 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2024 2565 2935 1186
Size: 838 541 370 4135
Fragments arranged by size:
4135 838 541 370
BseYI C'CCAG_C
Cuts at: 2952 3068 5087 5583 2952
Size: 116 2019 496 3253
Fragments arranged by size:
3253 2019 496 116
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2214 2244 2453 1163
Size: 573 478 30 209 4594
Fragments arranged by size:
4594 573 478 209 30
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3334 4095 5123 1540
Size: 275 186 1333 761 1028 2301
Fragments arranged by size:
2301 1333 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2915 2926 3945 4030 5101 2915
Size: 11 1019 85 1071 3698
Fragments arranged by size:
3698 1071 1019 85 11
BsmI GAATG_Cn'
Cuts at: 2205 2347 3395 3488 2205
Size: 142 1048 93 4601
Fragments arranged by size:
4601 1048 142 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2040 2661 2915 2926 3097 3203 3945
Size: 399 250 621 254 11 171 106 742
Cuts at: 3945 4030 5101 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 621 399 254 250 171
106 85 11
BspEI T'CCGG_A
Cuts at: 5547 5547
Size: 5884
BspHI T'CATG_A
Cuts at: 3773 5503 3773
Size: 1730 4154
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2432 3239 844
Size: 1588 807 3489
Fragments arranged by size:
3489 1588 807
BsrBI CCG'CTC
Cuts at: 1394 1823 3771 1394
Size: 429 1948 3507
Fragments arranged by size:
3507 1948 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2127 4379 4553 65
Size: 1183 879 2252 174 1396
Fragments arranged by size:
2252 1396 1183 879 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2248 2847 4528 4715 1700
Size: 110 438 599 1681 187 2869
Fragments arranged by size:
2869 1681 599 438 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5884
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5884
BssSI C'ACGA_G
Cuts at: 2894 3944 4956 2894
Size: 1050 1012 3822
Fragments arranged by size:
3822 1050 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5884
BstBI TT'CG_AA
Cuts at: 4688 4688
Size: 5884
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5884
BstYI r'GATC_y
Cuts at: 1972 2023 2997 3214 3581 3966 3983 5435 5521
Size: 51 974 217 367 385 17 1452 86
Cuts at: 5521 5533 5879 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 974 385 367 346 217 86
51 17 12
Bsu36I CC'TnA_GG
Cuts at: 2673 2673
Size: 5884
BtgI C'CryG_G
Cuts at: 513 1065 2004 2481 3001 513
Size: 552 939 477 520 3396
Fragments arranged by size:
3396 939 552 520 477
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2574 487
Size: 683 56 1348 3797
Fragments arranged by size:
3797 1348 683 56
BtsI GCAGTG_nn'
Cuts at: 2924 3400 4194 4214 2924
Size: 476 794 20 4594
Fragments arranged by size:
4594 794 476 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5884
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5849
DraI TTT'AAA
Cuts at: 3188 3188
Size: 5884
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2362 4891 809
Size: 507 1046 2529 1802
Fragments arranged by size:
2529 1802 1046 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2415 2484 3331
Size: 54 1425 50 275 18 585 69 847
Cuts at: 3331 4220 5849 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 847 585 275 69 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3331 1537
Size: 275 1519 4090
Fragments arranged by size:
4090 1519 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2856 3814 1353
Size: 1503 958 3423
Fragments arranged by size:
3423 1503 958
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4471 4845 4991 387
Size: 1064 138 2882 374 146 1280
Fragments arranged by size:
2882 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2190 2767 2978 3085 3131 3947 4544
Size: 393 558 577 211 107 46 816 597
Cuts at: 4544 5331 5599 1239
Size: 787 268 1524
Fragments arranged by size:
1524 816 787 597 577 558 393 268
211 107 46
EcoO109I rG'GnC_Cy
Cuts at: 3170 3170
Size: 5884
EcoRI G'AATT_C
Cuts at: 3193 3571 3193
Size: 378 5506
FspI TGC'GCA
Cuts at: 4390 4390
Size: 5884
HaeII r_GCGC'y
Cuts at: 1678 2530 2548 3016 5031 1678
Size: 852 18 468 2015 2531
Fragments arranged by size:
2531 2015 852 468 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2003 2035 4525 4557 4599
Size: 32 135 32 431 32 2490 32 42
Cuts at: 4599 4631 1373
Size: 32 2626
Fragments arranged by size:
2626 2490 431 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3228 3474 669
Size: 988 281 1290 246 3079
Fragments arranged by size:
3079 1290 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5884
HpaI GTT'AAC
Cuts at: 3474 3474
Size: 5884
KpnI G_GTAC'C
Cuts at: 3209 3209
Size: 5884
MfeI C'AATT_G
Cuts at: 3483 3483
Size: 5884
MluI A'CGCG_T
Cuts at: 5760 5760
Size: 5884
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4998 5182 1305
Size: 3693 184 2007
Fragments arranged by size:
3693 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2417 2486 10
Size: 54 1425 343 585 69 3408
Fragments arranged by size:
3408 1425 585 343 69 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3842 4201 4360 518
Size: 648 2676 359 159 2042
Fragments arranged by size:
2676 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2437 3300 3977 5125 5370 1606
Size: 831 863 677 1148 245 2120
Fragments arranged by size:
2120 1148 863 831 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2481 513
Size: 552 939 477 3916
Fragments arranged by size:
3916 939 552 477
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5884
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5884
NotI GC'GGCC_GC
Cuts at: 3331 3331
Size: 5884
NspI r_CATG'y
Cuts at: 1132 1229 2716 3242 4787 1132
Size: 97 1487 526 1545 2229
Fragments arranged by size:
2229 1545 1487 526 97
PasI CC'CwG_GG
Cuts at: 1348 2906 1348
Size: 1558 4326
PciI A'CATG_T
Cuts at: 2712 4783 2712
Size: 2071 3813
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2122 2975 1108
Size: 1014 853 4017
Fragments arranged by size:
4017 1014 853
PmeI GTTT'AAAC
Cuts at: 3188 3188
Size: 5884
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3953 3985 5466 5498 3953
Size: 32 1481 32 4339
Fragments arranged by size:
4339 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2178 1613
Size: 565 5319
PsiI TTA'TAA
Cuts at: 3454 3454
Size: 5884
PstI C_TGCA'G
Cuts at: 830 3236 830
Size: 2406 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5884
PvuII CAG'CTG
Cuts at: 2437 2437
Size: 5884
SalI G'TCGA_C
Cuts at: 1936 3226 1936
Size: 1290 4594
SbfI CC_TGCA'GG
Cuts at: 3236 3236
Size: 5884
ScaI AGT'ACT
Cuts at: 4132 5844 4132
Size: 1712 4172
SfcI C'TryA_G
Cuts at: 826 1045 3232 4367 5048 5239 826
Size: 219 2187 1135 681 191 1471
Fragments arranged by size:
2187 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5884
SgrAI Cr'CCGG_yG
Cuts at: 1700 2248 1700
Size: 548 5336
SmaI CCC'GGG
Cuts at: 1872 3211 1872
Size: 1339 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3053 3988 4889 5151 820
Size: 197 926 1110 935 901 262 1553
Fragments arranged by size:
1553 1110 935 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3675 493
Size: 3182 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5884
SphI G_CATG'C
Cuts at: 1132 3242 1132
Size: 2110 3774
SspI AAT'ATT
Cuts at: 5 52 2984 3808 5
Size: 47 2932 824 2081
Fragments arranged by size:
2932 2081 824 47
StuI AGG'CCT
Cuts at: 1517 3044 1517
Size: 1527 4357
StyI C'CwwG_G
Cuts at: 513 1065 2004 2481 3045 3323 513
Size: 552 939 477 564 278 3074
Fragments arranged by size:
3074 939 564 552 477 278
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3911 3928 4081 4266 4711 325
Size: 1255 2331 17 153 185 445 1498
Fragments arranged by size:
2331 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4130 5842 96
Size: 274 80 33 51 1443 2153 1712 138
Fragments arranged by size:
2153 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3098 3130 5844 5876 3098
Size: 32 2714 32 3106
Fragments arranged by size:
3106 2714 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5884
XbaI T'CTAG_A
Cuts at: 3220 3220
Size: 5884
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2271 2770 2271
Size: 499 5385
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5884
XmaI C'CCGG_G
Cuts at: 1870 3209 1870
Size: 1339 4545
XmnI GAAnn'nnTTC
Cuts at: 4013 4013
Size: 5884
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3692 276
Size: 53 83 186 936 2158 2468
Fragments arranged by size:
2468 2158 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI