Restriction map for
pFN21AB8723
(Circular) (Six-base) MAPSORT of: pFN21AB8723.seq Check: 6696 from: 1 to: 6232
pFN21AB8723 6232 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2249 2249
Size: 6232
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4042 278
Size: 53 83 186 936 2506 2468
Fragments arranged by size:
2506 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2541 2794 3575 1937
Size: 604 253 781 4594
Fragments arranged by size:
4594 781 604 253
Acc65I G'GTAC_C
Cuts at: 3362 3553 3362
Size: 191 6041
AclI AA'CG_TT
Cuts at: 1563 3531 4359 4732 1563
Size: 1968 828 373 3063
Fragments arranged by size:
3063 1968 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2129 2231 2663 4295 5679 5947 1632
Size: 497 102 432 1632 1384 268 1917
Fragments arranged by size:
1917 1632 1384 497 432 268 102
AflII C'TTAA_G
Cuts at: 820 1017 3235 820
Size: 197 2218 3817
Fragments arranged by size:
3817 2218 197
AflIII A'CryG_T
Cuts at: 1203 5131 6108 1203
Size: 3928 977 1327
Fragments arranged by size:
3928 1327 977
AgeI A'CCGG_T
Cuts at: 5063 5063
Size: 6232
AhdI GACnn_n'nnGTC
Cuts at: 4961 4961
Size: 6232
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6232
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6232
AlwNI CAG_nnn'CTG
Cuts at: 2104 2209 2896 3151 3975 5547 2104
Size: 105 687 255 824 1572 2789
Fragments arranged by size:
2789 1572 824 687 255 105
ApaLI G'TGCA_C
Cuts at: 4289 5445 4289
Size: 1156 5076
ApoI r'AATT_y
Cuts at: 1493 2756 3250 3541 3767 3919 6028 1493
Size: 1263 494 291 226 152 2109 1697
Fragments arranged by size:
2109 1697 1263 494 291 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2784 4786 160
Size: 872 1752 2002 1606
Fragments arranged by size:
2002 1752 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6232
AvaI C'yCGr_G
Cuts at: 1870 1943 3101 3557 1870
Size: 73 1158 456 4545
Fragments arranged by size:
4545 1158 456 73
BamHI G'GATC_C
Cuts at: 3562 3929 3562
Size: 367 5865
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2561 3362 3553 5008 618
Size: 325 217 627 774 801 191 1455 1842
Fragments arranged by size:
1842 1455 801 774 627 325 217 191
BanII G_rGCy'C
Cuts at: 1391 2160 3108 3504 3551 1391
Size: 769 948 396 47 4072
Fragments arranged by size:
4072 948 769 396 47
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5261
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4423 4457 1903
Size: 34 2486 34 3678
Fragments arranged by size:
3678 2486 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3336 4124 5334 3336
Size: 788 1210 4234
Fragments arranged by size:
4234 1210 788
BclI T'GATC_A
Cuts at: 1548 3181 1548
Size: 1633 4599
BfrBI ATG'CAT
Cuts at: 2819 3025 2819
Size: 206 6026
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4843 136
Size: 107 122 71 631 605 3171 1525
Fragments arranged by size:
3171 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6227 6227
Size: 6232
BlpI GC'TnA_GC
Cuts at: 2460 3302 3649 2460
Size: 842 347 5043
Fragments arranged by size:
5043 842 347
Bme1580I G_kGCm'C
Cuts at: 1790 3515 4293 5449 1790
Size: 1725 778 1156 2573
Fragments arranged by size:
2573 1725 1156 778
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6232
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2857 3963 4921
Size: 416 53 83 399 24 1440 1106 958
Cuts at: 4921 442
Size: 1753
Fragments arranged by size:
1753 1440 1106 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6232
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3148 3148
Size: 6232
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2159 2306 3128 3143 3359 3395 4892 1239
Size: 920 147 822 15 216 36 1497 2579
Fragments arranged by size:
2579 1497 920 822 216 147 36 15
Bpu10I CC'TnA_GC
Cuts at: 2954 3209 3404 2954
Size: 255 195 5782
Fragments arranged by size:
5782 255 195
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2537 4357 5222 5520 2537
Size: 1820 865 298 3249
Fragments arranged by size:
3249 1820 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2282 4895 882
Size: 617 783 2613 2219
Fragments arranged by size:
2613 2219 783 617
BsaAI yAC'GTr
Cuts at: 493 1204 2962 3319 4023 493
Size: 711 1758 357 704 2702
Fragments arranged by size:
2702 1758 711 704 357
BsaBI GATnn'nnATC
Cuts at: 6226 6226
Size: 6232
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4039 4421 5056 275
Size: 53 83 186 936 2506 382 635 1451
Fragments arranged by size:
2506 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4665 5063 5337 5484 5895 4665
Size: 398 274 147 411 5002
Fragments arranged by size:
5002 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3401 3431 1178
Size: 30 165 30 1998 30 3979
Fragments arranged by size:
3979 1998 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2789 2792 1186
Size: 1603 3 4626
Fragments arranged by size:
4626 1603 3
BseYI C'CCAG_C
Cuts at: 3041 3222 3434 5435 5931 3041
Size: 181 212 2001 496 3342
Fragments arranged by size:
3342 2001 496 212 181
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2243 2714 1163
Size: 573 507 471 4681
Fragments arranged by size:
4681 573 507 471
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3682 4443 5471 1540
Size: 275 186 1681 761 1028 2301
Fragments arranged by size:
2301 1681 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2160 2325 2501 2538 4293 4378 5449 2160
Size: 165 176 37 1755 85 1071 2943
Fragments arranged by size:
2943 1755 1071 176 165 85 37
BsmI GAATG_Cn'
Cuts at: 3743 3836 3743
Size: 93 6139
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2160 2325 2501 2538 3108 3504 3515
Size: 399 370 165 176 37 570 396 11
Cuts at: 3515 3551 4293 4378 5449 1391
Size: 36 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 570 399 396 370 176
165 85 37 36 11
BspEI T'CCGG_A
Cuts at: 5895 5895
Size: 6232
BspHI T'CATG_A
Cuts at: 4121 5851 4121
Size: 1730 4502
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2249 3587 844
Size: 1405 1338 3489
Fragments arranged by size:
3489 1405 1338
BsrBI CCG'CTC
Cuts at: 1394 1823 4119 1394
Size: 429 2296 3507
Fragments arranged by size:
3507 2296 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2127 2223 2661 4727 4901 65
Size: 1183 879 96 438 2066 174 1396
Fragments arranged by size:
2066 1396 1183 879 438 174 96
BsrFI r'CCGG_y
Cuts at: 1700 1810 3000 4876 5063 1700
Size: 110 1190 1876 187 2869
Fragments arranged by size:
2869 1876 1190 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6232
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6232
BssSI C'ACGA_G
Cuts at: 4292 5304 4292
Size: 1012 5220
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2104 2896 794
Size: 1310 792 4130
Fragments arranged by size:
4130 1310 792
BstBI TT'CG_AA
Cuts at: 5036 5036
Size: 6232
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6232
BstYI r'GATC_y
Cuts at: 1972 3562 3929 4314 4331 5783 5869 5881 6227
Size: 1590 367 385 17 1452 86 12 346
Cuts at: 6227 1972
Size: 1977
Fragments arranged by size:
1977 1590 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2072 3358 513
Size: 552 939 68 1286 3387
Fragments arranged by size:
3387 1286 939 552 68
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5493
Fragments arranged by size:
5493 683 56
BtsI GCAGTG_nn'
Cuts at: 2897 3214 3748 4542 4562 2897
Size: 317 534 794 20 4567
Fragments arranged by size:
4567 794 534 317 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6232
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6197
DraI TTT'AAA
Cuts at: 3536 3536
Size: 6232
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2410 5239 809
Size: 507 1094 2829 1802
Fragments arranged by size:
2829 1802 1094 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2124 3679 4568
Size: 54 1425 50 275 18 294 1555 889
Cuts at: 4568 6197 8
Size: 1629 43
Fragments arranged by size:
1629 1555 1425 889 294 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3679 1537
Size: 275 1867 4090
Fragments arranged by size:
4090 1867 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2165 2177 2841 4162 1353
Size: 812 12 664 1321 3423
Fragments arranged by size:
3423 1321 812 664 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4819 5193 5339 387
Size: 1064 138 3230 374 146 1280
Fragments arranged by size:
3230 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2158 2158
Size: 6232
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2129 2159 2231 2306 2663 3128 3143
Size: 393 497 30 72 75 357 465 15
Cuts at: 3143 3359 3395 4295 4892 5679 5947 1239
Size: 216 36 900 597 787 268 1524
Fragments arranged by size:
1524 900 787 597 497 465 393 357
268 216 75 72 36 30 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2108 2330 2108
Size: 222 6010
EcoO109I rG'GnC_Cy
Cuts at: 2119 3148 2119
Size: 1029 5203
EcoRI G'AATT_C
Cuts at: 3250 3541 3919 3250
Size: 291 378 5563
Fragments arranged by size:
5563 378 291
EcoRV GAT'ATC
Cuts at: 2671 2671
Size: 6232
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2321 2321
Size: 6232
FspI TGC'GCA
Cuts at: 4738 4738
Size: 6232
HaeII r_GCGC'y
Cuts at: 1678 5379 1678
Size: 3701 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2770 2787 2802 2819 4873
Size: 32 135 32 1198 17 15 17 2054
Cuts at: 4873 4905 4947 4979 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2054 1198 135 42 32 32 32
32 17 17 15
HincII GTy'rAC
Cuts at: 669 1657 1938 3576 3822 669
Size: 988 281 1638 246 3079
Fragments arranged by size:
3079 1638 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3238 748
Size: 2490 3742
HpaI GTT'AAC
Cuts at: 3822 3822
Size: 6232
KpnI G_GTAC'C
Cuts at: 3366 3557 3366
Size: 191 6041
MfeI C'AATT_G
Cuts at: 3831 3831
Size: 6232
MluI A'CGCG_T
Cuts at: 6108 6108
Size: 6232
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5346 5530 1305
Size: 4041 184 2007
Fragments arranged by size:
4041 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2126 10
Size: 54 1425 343 294 4116
Fragments arranged by size:
4116 1425 343 294 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2051 2057 2814 4190 4549 4708 518
Size: 648 885 6 757 1376 359 159 2042
Fragments arranged by size:
2042 1376 885 757 648 359 159 6
MspA1I CmG'CkG
Cuts at: 1606 2698 3648 4325 5473 5718 1606
Size: 1092 950 677 1148 245 2120
Fragments arranged by size:
2120 1148 1092 950 677 245
NaeI GCC'GGC
Cuts at: 3002 3002
Size: 6232
NcoI C'CATG_G
Cuts at: 513 1065 2004 3358 513
Size: 552 939 1354 3387
Fragments arranged by size:
3387 1354 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6232
NgoMIV G'CCGG_C
Cuts at: 3000 3000
Size: 6232
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6232
NotI GC'GGCC_GC
Cuts at: 3679 3679
Size: 6232
NsiI A_TGCA'T
Cuts at: 2821 3027 2821
Size: 206 6026
NspI r_CATG'y
Cuts at: 1132 1229 3398 3590 5135 1132
Size: 97 2169 192 1545 2229
Fragments arranged by size:
2229 2169 1545 192 97
PasI CC'CwG_GG
Cuts at: 1348 2151 1348
Size: 803 5429
PciI A'CATG_T
Cuts at: 5131 5131
Size: 6232
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2352 3151 1108
Size: 1244 799 4189
Fragments arranged by size:
4189 1244 799
PfoI T'CCnGG_A
Cuts at: 3144 3144
Size: 6232
PmeI GTTT'AAAC
Cuts at: 3536 3536
Size: 6232
PmlI CAC'GTG
Cuts at: 2962 3319 2962
Size: 357 5875
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4301 4333 5814 5846 4301
Size: 32 1481 32 4687
Fragments arranged by size:
4687 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3148 3148
Size: 6232
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6232
PsiI TTA'TAA
Cuts at: 3802 3802
Size: 6232
PstI C_TGCA'G
Cuts at: 830 2892 2901 3288 3584 830
Size: 2062 9 387 296 3478
Fragments arranged by size:
3478 2062 387 296 9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6232
PvuII CAG'CTG
Cuts at: 2698 2698
Size: 6232
SacI G_AGCT'C
Cuts at: 2160 2160
Size: 6232
SalI G'TCGA_C
Cuts at: 1936 3574 1936
Size: 1638 4594
SapI GCTCTTCn'nnn_
Cuts at: 2165 2841 2165
Size: 676 5556
SbfI CC_TGCA'GG
Cuts at: 2901 3584 2901
Size: 683 5549
ScaI AGT'ACT
Cuts at: 4480 6192 4480
Size: 1712 4520
SexAI A'CCwGG_T
Cuts at: 3150 3150
Size: 6232
SfcI C'TryA_G
Cuts at: 826 1045 2888 2897 3284 3580 4715 5396 5587
Size: 219 1843 9 387 296 1135 681 191
Cuts at: 5587 826
Size: 1471
Fragments arranged by size:
1843 1471 1135 681 387 296 219 191
9
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6232
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6232
SmaI CCC'GGG
Cuts at: 1872 3559 1872
Size: 1687 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2552 3235 4336 5237 5499 820
Size: 197 926 609 683 1101 901 262 1553
Fragments arranged by size:
1553 1101 926 901 683 609 262 197
SnaBI TAC'GTA
Cuts at: 493 4023 493
Size: 3530 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6232
SphI G_CATG'C
Cuts at: 1132 3398 3590 1132
Size: 2266 192 3774
Fragments arranged by size:
3774 2266 192
SspI AAT'ATT
Cuts at: 5 52 4156 5
Size: 47 4104 2081
Fragments arranged by size:
4104 2081 47
StuI AGG'CCT
Cuts at: 1517 2228 1517
Size: 711 5521
StyI C'CwwG_G
Cuts at: 513 1065 2004 2748 2973 3358 3671 513
Size: 552 939 744 225 385 313 3074
Fragments arranged by size:
3074 939 744 552 385 313 225
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3104 4259 4276 4429 4614 5059 325
Size: 1255 1524 1155 17 153 185 445 1498
Fragments arranged by size:
1524 1498 1255 1155 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2433 2617 4478
Size: 274 80 33 51 1443 456 184 1861
Cuts at: 4478 6190 96
Size: 1712 138
Fragments arranged by size:
1861 1712 1443 456 274 184 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2304 2336 3401 3433 6192 6224 2304
Size: 32 1065 32 2759 32 2312
Fragments arranged by size:
2759 2312 1065 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3152 1316
Size: 1836 4396
XbaI T'CTAG_A
Cuts at: 3568 3568
Size: 6232
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2134 2134
Size: 6232
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6232
XmaI C'CCGG_G
Cuts at: 1870 3557 1870
Size: 1687 4545
XmnI GAAnn'nnTTC
Cuts at: 4361 4361
Size: 6232
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4040 276
Size: 53 83 186 936 2506 2468
Fragments arranged by size:
2506 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII FseI FspAI
KasI NarI NruI PacI PspOMI PspXI PsrI RsrII
SacII SanDI SfiI SfoI SrfI SwaI