Restriction map for
pFN21AB8722
(Circular) (Six-base) MAPSORT of: pFN21AB8722.seq Check: 3012 from: 1 to: 6265
pFN21AB8722 6265 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4075 278
Size: 53 83 186 936 2539 2468
Fragments arranged by size:
2539 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2154 2379 3608 1937
Size: 217 225 1229 4594
Fragments arranged by size:
4594 1229 225 217
Acc65I G'GTAC_C
Cuts at: 3586 3586
Size: 6265
AclI AA'CG_TT
Cuts at: 1563 4392 4765 1563
Size: 2829 373 3063
Fragments arranged by size:
3063 2829 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3538 4328 5712 5980 1632
Size: 1906 790 1384 268 1917
Fragments arranged by size:
1917 1906 1384 790 268
AfeI AGC'GCT
Cuts at: 2746 2746
Size: 6265
AflII C'TTAA_G
Cuts at: 820 1017 2213 820
Size: 197 1196 4872
Fragments arranged by size:
4872 1196 197
AflIII A'CryG_T
Cuts at: 1203 5164 6141 1203
Size: 3961 977 1327
Fragments arranged by size:
3961 1327 977
AgeI A'CCGG_T
Cuts at: 5096 5096
Size: 6265
AhdI GACnn_n'nnGTC
Cuts at: 2260 4994 2260
Size: 2734 3531
AleI CACnn'nnGTG
Cuts at: 1166 3299 1166
Size: 2133 4132
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6265
AlwNI CAG_nnn'CTG
Cuts at: 3561 4008 5580 3561
Size: 447 1572 4246
Fragments arranged by size:
4246 1572 447
ApaI G_GGCC'C
Cuts at: 3123 3422 3123
Size: 299 5966
ApaLI G'TGCA_C
Cuts at: 4322 5478 4322
Size: 1156 5109
ApoI r'AATT_y
Cuts at: 1493 2101 3574 3800 3952 6061 1493
Size: 608 1473 226 152 2109 1697
Fragments arranged by size:
2109 1697 1473 608 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4819 160
Size: 872 3787 1606
Fragments arranged by size:
3787 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6265
AvaI C'yCGr_G
Cuts at: 1870 1943 2616 3590 1870
Size: 73 673 974 4545
Fragments arranged by size:
4545 974 673 73
BamHI G'GATC_C
Cuts at: 3595 3962 3595
Size: 367 5898
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2957 3586 5041 618
Size: 325 217 627 1170 629 1455 1842
Fragments arranged by size:
1842 1455 1170 629 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2451 2637 3123 3422 3495 3584 1391
Size: 1060 186 486 299 73 89 4072
Fragments arranged by size:
4072 1060 486 299 186 89 73
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3359 928
Size: 971 1460 3834
Fragments arranged by size:
3834 1460 971
BbvCI CC'TCA_GC
Cuts at: 3101 3101
Size: 6265
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4456 4490 1903
Size: 34 2519 34 3678
Fragments arranged by size:
3678 2519 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4157 5367 4157
Size: 1210 5055
BclI T'GATC_A
Cuts at: 1548 2268 1548
Size: 720 5545
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2390 4876 136
Size: 107 122 71 631 605 718 2486 1525
Fragments arranged by size:
2486 1525 718 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2086 6260 2086
Size: 4174 2091
BlpI GC'TnA_GC
Cuts at: 3682 3682
Size: 6265
Bme1580I G_kGCm'C
Cuts at: 1790 3123 3422 4326 5482 1790
Size: 1333 299 904 1156 2573
Fragments arranged by size:
2573 1333 1156 904 299
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6265
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2153 2309 3049
Size: 416 53 83 399 24 736 156 740
Cuts at: 3049 3528 3996 4954 442
Size: 479 468 958 1753
Fragments arranged by size:
1753 958 740 736 479 468 416 399
156 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6265
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2470 2470
Size: 6265
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2462 2903 4925 1239
Size: 1223 441 2022 2579
Fragments arranged by size:
2579 2022 1223 441
Bpu10I CC'TnA_GC
Cuts at: 3101 3101
Size: 6265
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3347 4390 5255 5553 3347
Size: 1043 865 298 4059
Fragments arranged by size:
4059 1043 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2606 2615 2771 3141 3371 4928 882
Size: 617 1107 9 156 370 230 1557 2219
Fragments arranged by size:
2219 1557 1107 617 370 230 156 9
BsaAI yAC'GTr
Cuts at: 493 1204 2848 4056 493
Size: 711 1644 1208 2702
Fragments arranged by size:
2702 1644 1208 711
BsaBI GATnn'nnATC
Cuts at: 6259 6259
Size: 6265
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4072 4454 5089 275
Size: 53 83 186 936 2539 382 635 1451
Fragments arranged by size:
2539 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2979 4698 5096 5370 5517 5928 2979
Size: 1719 398 274 147 411 3316
Fragments arranged by size:
3316 1719 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3542 3572 1178
Size: 30 165 30 2139 30 3871
Fragments arranged by size:
3871 2139 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3290 3335 1186
Size: 2104 45 4116
Fragments arranged by size:
4116 2104 45
BseYI C'CCAG_C
Cuts at: 2234 3460 5468 5964 2234
Size: 1226 2008 496 2535
Fragments arranged by size:
2535 2008 1226 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2149 2840 2873 1163
Size: 573 413 691 33 4555
Fragments arranged by size:
4555 691 573 413 33
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2370 3715 4476 5504 1540
Size: 275 186 369 1345 761 1028 2301
Fragments arranged by size:
2301 1345 1028 761 369 275 186
BsiHKAI G_wGCw'C
Cuts at: 2637 4326 4411 5482 2637
Size: 1689 85 1071 3420
Fragments arranged by size:
3420 1689 1071 85
BsmI GAATG_Cn'
Cuts at: 3776 3869 3776
Size: 93 6172
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2451 2637 3123 3422 3495 3584 4326
Size: 399 661 186 486 299 73 89 742
Cuts at: 4326 4411 5482 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 661 486 399 299 186
89 85 73
BspEI T'CCGG_A
Cuts at: 2979 5928 2979
Size: 2949 3316
BspHI T'CATG_A
Cuts at: 4154 5884 4154
Size: 1730 4535
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2442 2921 3620 844
Size: 1598 479 699 3489
Fragments arranged by size:
3489 1598 699 479
BsrBI CCG'CTC
Cuts at: 1394 1823 3349 4152 1394
Size: 429 1526 803 3507
Fragments arranged by size:
3507 1526 803 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2245 4760 4934 65
Size: 1183 997 2515 174 1396
Fragments arranged by size:
2515 1396 1183 997 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2369 4909 5096 1700
Size: 110 559 2540 187 2869
Fragments arranged by size:
2869 2540 559 187 110
BsrGI T'GTAC_A
Cuts at: 96 2037 96
Size: 1941 4324
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6265
BssSI C'ACGA_G
Cuts at: 4325 5337 4325
Size: 1012 5253
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6265
BstBI TT'CG_AA
Cuts at: 5069 5069
Size: 6265
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6265
BstXI CCAn_nnnn'nTGG
Cuts at: 2494 2767 2494
Size: 273 5992
BstYI r'GATC_y
Cuts at: 1972 2086 2972 3279 3595 3962 4347 4364 5816
Size: 114 886 307 316 367 385 17 1452
Cuts at: 5816 5902 5914 6260 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 886 385 367 346 316 307
114 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3270 3270
Size: 6265
BtgI C'CryG_G
Cuts at: 513 1065 2004 2207 3091 3231 513
Size: 552 939 203 884 140 3547
Fragments arranged by size:
3547 939 884 552 203 140
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2780 487
Size: 683 56 1554 3972
Fragments arranged by size:
3972 1554 683 56
BtsI GCAGTG_nn'
Cuts at: 2125 2308 2400 2890 3781 4575 4595 2125
Size: 183 92 490 891 794 20 3795
Fragments arranged by size:
3795 891 794 490 183 92 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6265
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6230
DraI TTT'AAA
Cuts at: 3569 3569
Size: 6265
DraIII CAC_nnn'GTG
Cuts at: 2353 2353
Size: 6265
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5272 809
Size: 507 3956 1802
Fragments arranged by size:
3956 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3108 3712 4601
Size: 54 1425 50 275 18 1278 604 889
Cuts at: 4601 6230 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1278 889 604 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3712 1537
Size: 275 1900 4090
Fragments arranged by size:
4090 1900 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2556 3383 3483 4195 1353
Size: 1203 827 100 712 3423
Fragments arranged by size:
3423 1203 827 712 100
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3422 4852 5226 5372 387
Size: 1064 138 1833 1430 374 146 1280
Fragments arranged by size:
1833 1430 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2635 2635
Size: 6265
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2462 2903 3538 4328 4925 5712 5980
Size: 393 830 441 635 790 597 787 268
Cuts at: 5980 1239
Size: 1524
Fragments arranged by size:
1524 830 790 787 635 597 441 393
268
EcoO109I rG'GnC_Cy
Cuts at: 2262 2431 2482 2578 2683 3418 3419 2262
Size: 169 51 96 105 735 1 5108
Fragments arranged by size:
5108 735 169 105 96 51 1
EcoRI G'AATT_C
Cuts at: 3574 3952 3574
Size: 378 5887
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2296 2296
Size: 6265
FspI TGC'GCA
Cuts at: 3342 4771 3342
Size: 1429 4836
HaeII r_GCGC'y
Cuts at: 1678 2748 5412 1678
Size: 1070 2664 2531
Fragments arranged by size:
2664 2531 1070
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2246 2278 3542 3574 4906
Size: 32 135 32 674 32 1264 32 1332
Cuts at: 4906 4938 4980 5012 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1332 1264 674 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3609 3855 669
Size: 988 281 1671 246 3079
Fragments arranged by size:
3079 1671 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6265
HpaI GTT'AAC
Cuts at: 3855 3855
Size: 6265
KpnI G_GTAC'C
Cuts at: 3590 3590
Size: 6265
MfeI C'AATT_G
Cuts at: 3864 3864
Size: 6265
MluI A'CGCG_T
Cuts at: 6141 6141
Size: 6265
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5379 5563 1305
Size: 4074 184 2007
Fragments arranged by size:
4074 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3110 10
Size: 54 1425 343 1278 3165
Fragments arranged by size:
3165 1425 1278 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2067 2765 3299 4223 4582 4741 518
Size: 648 901 698 534 924 359 159 2042
Fragments arranged by size:
2042 924 901 698 648 534 359 159
MspA1I CmG'CkG
Cuts at: 1606 2795 3093 3681 4358 5506 5751 1606
Size: 1189 298 588 677 1148 245 2120
Fragments arranged by size:
2120 1189 1148 677 588 298 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2207 3231 513
Size: 552 939 203 1024 3547
Fragments arranged by size:
3547 1024 939 552 203
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6265
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6265
NotI GC'GGCC_GC
Cuts at: 3712 3712
Size: 6265
NspI r_CATG'y
Cuts at: 1132 1229 3623 5168 1132
Size: 97 2394 1545 2229
Fragments arranged by size:
2394 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6265
PciI A'CATG_T
Cuts at: 5164 5164
Size: 6265
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2353 3442 1108
Size: 1245 1089 3931
Fragments arranged by size:
3931 1245 1089
PfoI T'CCnGG_A
Cuts at: 2427 2478 2772 2427
Size: 51 294 5920
Fragments arranged by size:
5920 294 51
PmeI GTTT'AAAC
Cuts at: 3569 3569
Size: 6265
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4334 4366 5847 5879 4334
Size: 32 1481 32 4720
Fragments arranged by size:
4720 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2262 2431 2482 2578 2262
Size: 169 51 96 5949
Fragments arranged by size:
5949 169 96 51
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6265
PsiI TTA'TAA
Cuts at: 3835 3835
Size: 6265
PspOMI G'GGCC_C
Cuts at: 3119 3418 3119
Size: 299 5966
PstI C_TGCA'G
Cuts at: 830 2132 2145 2631 3617 830
Size: 1302 13 486 986 3478
Fragments arranged by size:
3478 1302 986 486 13
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6265
PvuII CAG'CTG
Cuts at: 2795 2795
Size: 6265
SacI G_AGCT'C
Cuts at: 2637 2637
Size: 6265
SacII CC_GC'GG
Cuts at: 3094 3094
Size: 6265
SalI G'TCGA_C
Cuts at: 1936 3607 1936
Size: 1671 4594
SapI GCTCTTCn'nnn_
Cuts at: 3383 3483 3383
Size: 100 6165
SbfI CC_TGCA'GG
Cuts at: 2631 3617 2631
Size: 986 5279
ScaI AGT'ACT
Cuts at: 4513 6225 4513
Size: 1712 4553
SfcI C'TryA_G
Cuts at: 826 1045 2128 2141 2627 3297 3613 4748 5429
Size: 219 1083 13 486 670 316 1135 681
Cuts at: 5429 5620 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1083 681 670 486 316 219
191 13
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6265
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6265
SmaI CCC'GGG
Cuts at: 1872 3592 1872
Size: 1720 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2213 3362 4369 5270 5532 820
Size: 197 926 270 1149 1007 901 262 1553
Fragments arranged by size:
1553 1149 1007 926 901 270 262 197
SnaBI TAC'GTA
Cuts at: 493 4056 493
Size: 3563 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6265
SphI G_CATG'C
Cuts at: 1132 3623 1132
Size: 2491 3774
SspI AAT'ATT
Cuts at: 5 52 4189 5
Size: 47 4137 2081
Fragments arranged by size:
4137 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6265
StyI C'CwwG_G
Cuts at: 513 1065 2004 2207 2995 3231 3704 513
Size: 552 939 203 788 236 473 3074
Fragments arranged by size:
3074 939 788 552 473 236 203
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2360 2456 4292 4309 4462 4647 5092
Size: 1255 780 96 1836 17 153 185 445
Cuts at: 5092 325
Size: 1498
Fragments arranged by size:
1836 1498 1255 780 445 185 153 96
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2037 3450 4511
Size: 274 80 33 51 1443 60 1413 1061
Cuts at: 4511 6223 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1413 1061 274 138 80 60
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6225 6257 6225
Size: 32 6233
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6265
XbaI T'CTAG_A
Cuts at: 3601 3601
Size: 6265
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3238 3238
Size: 6265
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6265
XmaI C'CCGG_G
Cuts at: 1870 3590 1870
Size: 1720 4545
XmnI GAAnn'nnTTC
Cuts at: 3050 4394 3050
Size: 1344 4921
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4073 276
Size: 53 83 186 936 2539 2468
Fragments arranged by size:
2539 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SacII SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstZ17I EcoNI EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI
RsrII SanDI SexAI SfiI SfoI SrfI SwaI