Restriction map for
pFN21AB8721
(Circular) (Six-base) MAPSORT of: pFN21AB8721.seq Check: 3184 from: 1 to: 6376
pFN21AB8721 6376 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2109 3124 2109
Size: 1015 5361
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4186 278
Size: 53 83 186 936 2650 2468
Fragments arranged by size:
2650 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2901 3180 3719 1937
Size: 964 279 539 4594
Fragments arranged by size:
4594 964 539 279
Acc65I G'GTAC_C
Cuts at: 3697 3697
Size: 6376
AclI AA'CG_TT
Cuts at: 1563 3675 4503 4876 1563
Size: 2112 828 373 3063
Fragments arranged by size:
3063 2112 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2007 2393 3359 3471 4439 5823 6091 1632
Size: 375 386 966 112 968 1384 268 1917
Fragments arranged by size:
1917 1384 968 966 386 375 268 112
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6179
AflIII A'CryG_T
Cuts at: 1203 5275 6252 1203
Size: 4072 977 1327
Fragments arranged by size:
4072 1327 977
AgeI A'CCGG_T
Cuts at: 5207 5207
Size: 6376
AhdI GACnn_n'nnGTC
Cuts at: 5105 5105
Size: 6376
AleI CACnn'nnGTG
Cuts at: 1166 3420 1166
Size: 2254 4122
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6376
AlwNI CAG_nnn'CTG
Cuts at: 2047 2131 2371 3067 3157 3239 3527 4119 5691
Size: 84 240 696 90 82 288 592 1572
Cuts at: 5691 2047
Size: 2732
Fragments arranged by size:
2732 1572 696 592 288 240 90 84
82
ApaI G_GGCC'C
Cuts at: 2242 2497 3303 3337 2242
Size: 255 806 34 5281
Fragments arranged by size:
5281 806 255 34
ApaLI G'TGCA_C
Cuts at: 2209 3413 4433 5589 2209
Size: 1204 1020 1156 2996
Fragments arranged by size:
2996 1204 1156 1020
ApoI r'AATT_y
Cuts at: 1493 3685 3911 4063 6172 1493
Size: 2192 226 152 2109 1697
Fragments arranged by size:
2192 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2874 4930 160
Size: 872 1842 2056 1606
Fragments arranged by size:
2056 1842 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6376
AvaI C'yCGr_G
Cuts at: 1870 1943 2071 2630 2662 3701 1870
Size: 73 128 559 32 1039 4545
Fragments arranged by size:
4545 1039 559 128 73 32
BamHI G'GATC_C
Cuts at: 3706 4073 3706
Size: 367 6009
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2717 3697 5152 618
Size: 325 217 627 930 980 1455 1842
Fragments arranged by size:
1842 1455 980 930 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2072 2097 2242 2497 3303 3337 3695 1391
Size: 681 25 145 255 806 34 358 4072
Fragments arranged by size:
4072 806 681 358 255 145 34 25
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3626 928
Size: 971 1727 3678
Fragments arranged by size:
3678 1727 971
BbvCI CC'TCA_GC
Cuts at: 3528 3528
Size: 6376
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2305 2339 4567 4601 1903
Size: 34 368 34 2228 34 3678
Fragments arranged by size:
3678 2228 368 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4268 5478 4268
Size: 1210 5166
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6376
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2044 2166 2570
Size: 107 122 71 631 605 372 122 404
Cuts at: 2570 2726 3307 4987 136
Size: 156 581 1680 1525
Fragments arranged by size:
1680 1525 631 605 581 404 372 156
122 122 107 71
BglII A'GATC_T
Cuts at: 3597 6371 3597
Size: 2774 3602
BlpI GC'TnA_GC
Cuts at: 2105 3376 3793 2105
Size: 1271 417 4688
Fragments arranged by size:
4688 1271 417
Bme1580I G_kGCm'C
Cuts at: 1790 2213 2242 2497 3303 3337 3417 4437 5593
Size: 423 29 255 806 34 80 1020 1156
Cuts at: 5593 1790
Size: 2573
Fragments arranged by size:
2573 1156 1020 806 423 255 80 34
29
BmgBI CAC'GTC
Cuts at: 1318 3391 1318
Size: 2073 4303
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3297 4107 5065
Size: 416 53 83 399 24 1880 810 958
Cuts at: 5065 442
Size: 1753
Fragments arranged by size:
1880 1753 958 810 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2861 1056
Size: 1805 4571
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2051 2264 2270 2663 5036 1239
Size: 812 213 6 393 2373 2579
Fragments arranged by size:
2579 2373 812 393 213 6
Bpu10I CC'TnA_GC
Cuts at: 2604 3528 2604
Size: 924 5452
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4501 5366 5664 4501
Size: 865 298 5213
Fragments arranged by size:
5213 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2903 2964 5039 882
Size: 617 1404 61 2075 2219
Fragments arranged by size:
2219 2075 1404 617 61
BsaAI yAC'GTr
Cuts at: 493 1204 4167 493
Size: 711 2963 2702
Fragments arranged by size:
2963 2702 711
BsaBI GATnn'nnATC
Cuts at: 2969 6370 2969
Size: 3401 2975
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4183 4565 5200 275
Size: 53 83 186 936 2650 382 635 1451
Fragments arranged by size:
2650 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4809 5207 5481 5628 6039 4809
Size: 398 274 147 411 5146
Fragments arranged by size:
5146 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2190 2220 1178
Size: 30 165 30 787 30 5334
Fragments arranged by size:
5334 787 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2089 2102 2105 2261 2648 2921 1186
Size: 903 13 3 156 387 273 4641
Fragments arranged by size:
4641 903 387 273 156 13 3
BseYI C'CCAG_C
Cuts at: 2040 2257 2568 3067 3239 5579 6075 2040
Size: 217 311 499 172 2340 496 2341
Fragments arranged by size:
2341 2340 499 496 311 217 172
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3186 1163
Size: 573 1450 4353
Fragments arranged by size:
4353 1450 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2170 3826 4587 5615 1540
Size: 275 186 169 1656 761 1028 2301
Fragments arranged by size:
2301 1656 1028 761 275 186 169
BsiHKAI G_wGCw'C
Cuts at: 2213 2256 3417 4437 4522 5593 2213
Size: 43 1161 1020 85 1071 2996
Fragments arranged by size:
2996 1161 1071 1020 85 43
BsmI GAATG_Cn'
Cuts at: 3314 3373 3887 3980 3314
Size: 59 514 93 5710
Fragments arranged by size:
5710 514 93 59
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2072 2097 2213 2242 2256 2497 3303
Size: 399 282 25 116 29 14 241 806
Cuts at: 3303 3337 3417 3695 4437 4522 5593 1391
Size: 34 80 278 742 85 1071 2174
Fragments arranged by size:
2174 1071 806 742 399 282 278 241
116 85 80 34 29 25 14
BspEI T'CCGG_A
Cuts at: 6039 6039
Size: 6376
BspHI T'CATG_A
Cuts at: 4265 5995 4265
Size: 1730 4646
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2109 3124 3731 844
Size: 1265 1015 607 3489
Fragments arranged by size:
3489 1265 1015 607
BsrBI CCG'CTC
Cuts at: 1394 1823 4263 1394
Size: 429 2440 3507
Fragments arranged by size:
3507 2440 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4871 5045 65
Size: 1183 3623 174 1396
Fragments arranged by size:
3623 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2169 2378 5020 5207 1700
Size: 110 359 209 2642 187 2869
Fragments arranged by size:
2869 2642 359 209 187 110
BsrGI T'GTAC_A
Cuts at: 96 3104 96
Size: 3008 3368
BssHII G'CGCG_C
Cuts at: 1925 2622 1925
Size: 697 5679
BssSI C'ACGA_G
Cuts at: 3545 4436 5448 3545
Size: 891 1012 4473
Fragments arranged by size:
4473 1012 891
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3368 794
Size: 2574 3802
BstBI TT'CG_AA
Cuts at: 5180 5180
Size: 6376
BstEII G'GTnAC_C
Cuts at: 1184 2185 1184
Size: 1001 5375
BstXI CCAn_nnnn'nTGG
Cuts at: 2265 2554 3253 2265
Size: 289 699 5388
Fragments arranged by size:
5388 699 289
BstYI r'GATC_y
Cuts at: 1972 2482 3217 3597 3706 4073 4458 4475 5927
Size: 510 735 380 109 367 385 17 1452
Cuts at: 5927 6013 6025 6371 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 735 510 385 380 367 346
109 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2234 2234
Size: 6376
BtgI C'CryG_G
Cuts at: 513 1065 2004 2164 2415 2532 3184 513
Size: 552 939 160 251 117 652 3705
Fragments arranged by size:
3705 939 652 552 251 160 117
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5637
Fragments arranged by size:
5637 683 56
BtsI GCAGTG_nn'
Cuts at: 2223 2421 2441 2889 3528 3892 4686 4706 2223
Size: 198 20 448 639 364 794 20 3893
Fragments arranged by size:
3893 794 639 448 364 198 20 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6376
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6341
DraI TTT'AAA
Cuts at: 3680 3680
Size: 6376
DraIII CAC_nnn'GTG
Cuts at: 2217 2217
Size: 6376
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2015 5383 809
Size: 507 699 3368 1802
Fragments arranged by size:
3368 1802 699 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2133 2167 2418
Size: 54 1425 50 275 18 303 34 251
Cuts at: 2418 2436 2454 2700 3570 3823 4712 6341 8
Size: 18 18 246 870 253 889 1629 43
Fragments arranged by size:
1629 1425 889 870 303 275 253 251
246 54 50 43 34 18 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 2167 3823 1537
Size: 275 355 1656 4090
Fragments arranged by size:
4090 1656 355 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2079 4306 1353
Size: 726 2227 3423
Fragments arranged by size:
3423 2227 726
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2939 4963 5337 5483 387
Size: 1064 138 1350 2024 374 146 1280
Fragments arranged by size:
2024 1350 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2007 2051 2264 2270 2393 2663 3359
Size: 393 375 44 213 6 123 270 696
Cuts at: 3359 3471 4439 5036 5823 6091 1239
Size: 112 968 597 787 268 1524
Fragments arranged by size:
1524 968 787 696 597 393 375 270
268 213 123 112 44 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2984 2984
Size: 6376
EcoO109I rG'GnC_Cy
Cuts at: 2238 2239 2431 2471 2494 3299 3334 2238
Size: 1 192 40 23 805 35 5280
Fragments arranged by size:
5280 805 192 40 35 23 1
EcoRI G'AATT_C
Cuts at: 3685 4063 3685
Size: 378 5998
FseI GG_CCGG'CC
Cuts at: 2382 2382
Size: 6376
FspI TGC'GCA
Cuts at: 4882 4882
Size: 6376
HaeII r_GCGC'y
Cuts at: 1678 5523 1678
Size: 3845 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3589 3621 5017 5049 5091
Size: 32 135 32 2017 32 1396 32 42
Cuts at: 5091 5123 1373
Size: 32 2626
Fragments arranged by size:
2626 2017 1396 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2902 3720 3966 669
Size: 988 281 964 818 246 3079
Fragments arranged by size:
3079 988 964 818 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6376
HpaI GTT'AAC
Cuts at: 3966 3966
Size: 6376
KpnI G_GTAC'C
Cuts at: 3701 3701
Size: 6376
MfeI C'AATT_G
Cuts at: 3975 3975
Size: 6376
MluI A'CGCG_T
Cuts at: 6252 6252
Size: 6376
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2973 5490 5674 1305
Size: 1668 2517 184 2007
Fragments arranged by size:
2517 2007 1668 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2135 2438 2456 2702 3572
Size: 54 1425 343 303 303 18 246 870
Cuts at: 3572 10
Size: 2814
Fragments arranged by size:
2814 1425 870 343 303 303 246 54
18
MslI CAynn'nnrTG
Cuts at: 518 1166 2763 2942 3251 3420 4334 4693 4852
Size: 648 1597 179 309 169 914 359 159
Cuts at: 4852 518
Size: 2042
Fragments arranged by size:
2042 1597 914 648 359 309 179 169
159
MspA1I CmG'CkG
Cuts at: 1606 2044 2368 2864 3792 4469 5617 5862 1606
Size: 438 324 496 928 677 1148 245 2120
Fragments arranged by size:
2120 1148 928 677 496 438 324 245
NaeI GCC'GGC
Cuts at: 2171 2380 2171
Size: 209 6167
NcoI C'CATG_G
Cuts at: 513 1065 2004 2532 513
Size: 552 939 528 4357
Fragments arranged by size:
4357 939 552 528
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6376
NgoMIV G'CCGG_C
Cuts at: 2169 2378 2169
Size: 209 6167
NheI G'CTAG_C
Cuts at: 1052 2857 1052
Size: 1805 4571
NotI GC'GGCC_GC
Cuts at: 3823 3823
Size: 6376
NspI r_CATG'y
Cuts at: 1132 1229 3734 5279 1132
Size: 97 2505 1545 2229
Fragments arranged by size:
2505 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3151 1348
Size: 1803 4573
PciI A'CATG_T
Cuts at: 5275 5275
Size: 6376
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2047 2131 2467 3239 1108
Size: 939 84 336 772 4245
Fragments arranged by size:
4245 939 772 336 84
PfoI T'CCnGG_A
Cuts at: 2274 2400 3232 2274
Size: 126 832 5418
Fragments arranged by size:
5418 832 126
PmeI GTTT'AAAC
Cuts at: 3680 3680
Size: 6376
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4445 4477 5958 5990 4445
Size: 32 1481 32 4831
Fragments arranged by size:
4831 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2431 2431
Size: 6376
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6376
PsiI TTA'TAA
Cuts at: 3946 3946
Size: 6376
PspOMI G'GGCC_C
Cuts at: 2238 2493 3299 3333 2238
Size: 255 806 34 5281
Fragments arranged by size:
5281 806 255 34
PstI C_TGCA'G
Cuts at: 830 2122 2428 2869 3728 830
Size: 1292 306 441 859 3478
Fragments arranged by size:
3478 1292 859 441 306
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6376
PvuII CAG'CTG
Cuts at: 2368 2864 2368
Size: 496 5880
SalI G'TCGA_C
Cuts at: 1936 2900 3718 1936
Size: 964 818 4594
Fragments arranged by size:
4594 964 818
SbfI CC_TGCA'GG
Cuts at: 3728 3728
Size: 6376
ScaI AGT'ACT
Cuts at: 4624 6336 4624
Size: 1712 4664
SfcI C'TryA_G
Cuts at: 826 1045 2118 2424 2865 3539 3552 3724 4859
Size: 219 1073 306 441 674 13 172 1135
Cuts at: 4859 5540 5731 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1073 681 674 441 306 219
191 172 13
SfiI GGCCn_nnn'nGGCC
Cuts at: 2166 3307 2166
Size: 1141 5235
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6376
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6376
SmaI CCC'GGG
Cuts at: 1872 2632 2664 3703 1872
Size: 760 32 1039 4545
Fragments arranged by size:
4545 1039 760 32
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4480 5381 5643 820
Size: 197 926 2537 901 262 1553
Fragments arranged by size:
2537 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4167 493
Size: 3674 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6376
SphI G_CATG'C
Cuts at: 1132 3734 1132
Size: 2602 3774
SspI AAT'ATT
Cuts at: 5 52 4300 5
Size: 47 4248 2081
Fragments arranged by size:
4248 2081 47
StuI AGG'CCT
Cuts at: 1517 2408 1517
Size: 891 5485
StyI C'CwwG_G
Cuts at: 513 1065 2004 2060 2532 3418 3508 3661 3815
Size: 552 939 56 472 886 90 153 154
Cuts at: 3815 513
Size: 3074
Fragments arranged by size:
3074 939 886 552 472 154 153 90
56
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2271 3164 3428 3648 4403 4420 4573
Size: 1255 691 893 264 220 755 17 153
Cuts at: 4573 4758 5203 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 893 755 691 445 264 220
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3104 4622 6334
Size: 274 80 33 51 1443 1127 1518 1712
Cuts at: 6334 96
Size: 138
Fragments arranged by size:
1712 1518 1443 1127 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2190 2222 2510 2542 2674 2706 2862 2894 3467
Size: 32 288 32 132 32 156 32 573
Cuts at: 3467 3499 6336 6368 2190
Size: 32 2837 32 2198
Fragments arranged by size:
2837 2198 573 288 156 132 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6376
XbaI T'CTAG_A
Cuts at: 3712 3712
Size: 6376
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2464 2464
Size: 6376
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6376
XmaI C'CCGG_G
Cuts at: 1870 2630 2662 3701 1870
Size: 760 32 1039 4545
Fragments arranged by size:
4545 1039 760 32
XmnI GAAnn'nnTTC
Cuts at: 4505 4505
Size: 6376
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4184 276
Size: 53 83 186 936 2650 2468
Fragments arranged by size:
2650 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SbfI ScaI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstZ17I EcoICRI EcoRV FalI FspAI KasI
NarI NruI NsiI PacI PmlI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfoI SrfI SwaI