Restriction map for
pFN21AB8720
(Circular) (Six-base) MAPSORT of: pFN21AB8720.seq Check: 3584 from: 1 to: 6865
pFN21AB8720 6865 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4675 278
Size: 53 83 186 936 3139 2468
Fragments arranged by size:
3139 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4208 1937
Size: 2271 4594
Acc65I G'GTAC_C
Cuts at: 4186 4186
Size: 6865
AclI AA'CG_TT
Cuts at: 1563 4992 5365 1563
Size: 3429 373 3063
Fragments arranged by size:
3429 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2346 2903 4928 6312 6580 1632
Size: 714 557 2025 1384 268 1917
Fragments arranged by size:
2025 1917 1384 714 557 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6668
AflIII A'CryG_T
Cuts at: 1203 3982 5764 6741 1203
Size: 2779 1782 977 1327
Fragments arranged by size:
2779 1782 1327 977
AgeI A'CCGG_T
Cuts at: 4069 5696 4069
Size: 1627 5238
AhdI GACnn_n'nnGTC
Cuts at: 5594 5594
Size: 6865
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6865
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6865
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4130 4162 4130
Size: 32 6833
AlwNI CAG_nnn'CTG
Cuts at: 3113 3238 3923 4608 6180 3113
Size: 125 685 685 1572 3798
Fragments arranged by size:
3798 1572 685 685 125
ApaI G_GGCC'C
Cuts at: 2475 2475
Size: 6865
ApaLI G'TGCA_C
Cuts at: 2974 3436 4922 6078 2974
Size: 462 1486 1156 3761
Fragments arranged by size:
3761 1486 1156 462
ApoI r'AATT_y
Cuts at: 1493 2051 3011 3316 3360 4174 4400 4552 6661
Size: 558 960 305 44 814 226 152 2109
Cuts at: 6661 1493
Size: 1697
Fragments arranged by size:
2109 1697 960 814 558 305 226 152
44
AseI AT'TA_AT
Cuts at: 160 1032 5419 160
Size: 872 4387 1606
Fragments arranged by size:
4387 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6865
AvaI C'yCGr_G
Cuts at: 1870 1943 2547 2639 2661 2800 2941 3831 4190
Size: 73 604 92 22 139 141 890 359
Cuts at: 4190 1870
Size: 4545
Fragments arranged by size:
4545 890 604 359 141 139 92 73
22
BamHI G'GATC_C
Cuts at: 4195 4562 4195
Size: 367 6498
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2528 2551 3181 4186 5641
Size: 325 217 627 741 23 630 1005 1455
Cuts at: 5641 618
Size: 1842
Fragments arranged by size:
1842 1455 1005 741 630 627 325 217
23
BanII G_rGCy'C
Cuts at: 1391 2222 2475 3611 3651 4100 4121 4184 1391
Size: 831 253 1136 40 449 21 63 4072
Fragments arranged by size:
4072 1136 831 449 253 63 40 21
BbeI G_GCGC'C
Cuts at: 2555 2555
Size: 6865
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2031 2067 2098 2334 2894 928
Size: 971 132 36 31 236 560 4899
Fragments arranged by size:
4899 971 560 236 132 36 31
BbvCI CC'TCA_GC
Cuts at: 3190 3190
Size: 6865
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5056 5090 1903
Size: 34 3119 34 3678
Fragments arranged by size:
3678 3119 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4757 5967 4757
Size: 1210 5655
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6865
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2838 5476 136
Size: 107 122 71 631 605 1166 2638 1525
Fragments arranged by size:
2638 1525 1166 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6860 6860
Size: 6865
BlpI GC'TnA_GC
Cuts at: 3512 4115 4282 3512
Size: 603 167 6095
Fragments arranged by size:
6095 603 167
Bme1580I G_kGCm'C
Cuts at: 1790 2475 2533 2978 3421 3440 4926 6082 1790
Size: 685 58 445 443 19 1486 1156 2573
Fragments arranged by size:
2573 1486 1156 685 445 443 58 19
BmgBI CAC'GTC
Cuts at: 1318 2424 3441 1318
Size: 1106 1017 4742
Fragments arranged by size:
4742 1106 1017
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4596 5554 442
Size: 416 53 83 399 24 3179 958 1753
Fragments arranged by size:
3179 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6865
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4119 4119
Size: 6865
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2236 3229 3765 3900 4020 5525 1239
Size: 997 993 536 135 120 1505 2579
Fragments arranged by size:
2579 1505 997 993 536 135 120
Bpu10I CC'TnA_GC
Cuts at: 3190 4094 3190
Size: 904 5961
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4142 4990 5855 6153 4142
Size: 848 865 298 4854
Fragments arranged by size:
4854 865 848 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5528 882
Size: 617 4029 2219
Fragments arranged by size:
4029 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3985 4656 493
Size: 711 2781 671 2702
Fragments arranged by size:
2781 2702 711 671
BsaBI GATnn'nnATC
Cuts at: 6859 6859
Size: 6865
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2552 4672 5054 5689
Size: 53 83 186 936 1019 2120 382 635
Cuts at: 5689 275
Size: 1451
Fragments arranged by size:
2120 1451 1019 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2167 3217 4069 5298 5696 5970 6117 6528 2167
Size: 1050 852 1229 398 274 147 411 2504
Fragments arranged by size:
2504 1229 1050 852 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4018 4048 4082 4112 1178
Size: 30 165 30 2615 30 34 30 3931
Fragments arranged by size:
3931 2615 165 34 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2494 2929 3735 4145 1186
Size: 1308 435 806 410 3906
Fragments arranged by size:
3906 1308 806 435 410
BseYI C'CCAG_C
Cuts at: 2467 2519 2752 2983 6068 6564 2467
Size: 52 233 231 3085 496 2768
Fragments arranged by size:
3085 2768 496 233 231 52
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6292
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2823 3428 4315 5076 6104 1540
Size: 275 186 822 605 887 761 1028 2301
Fragments arranged by size:
2301 1028 887 822 761 605 275 186
BsiHKAI G_wGCw'C
Cuts at: 2978 3440 4100 4926 5011 6082 2978
Size: 462 660 826 85 1071 3761
Fragments arranged by size:
3761 1071 826 660 462 85
BsmI GAATG_Cn'
Cuts at: 4376 4469 4376
Size: 93 6772
BsmBI CGTCTCn'nnnn_
Cuts at: 2430 3628 2430
Size: 1198 5667
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2222 2475 2533 2978 3421 3440 3611
Size: 399 432 253 58 445 443 19 171
Cuts at: 3611 3651 4100 4121 4184 4926 5011 6082 1391
Size: 40 449 21 63 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 449 445 443 432 399
253 171 85 63 58 40 21 19
BspEI T'CCGG_A
Cuts at: 3217 6528 3217
Size: 3311 3554
BspHI T'CATG_A
Cuts at: 4023 4754 6484 4023
Size: 731 1730 4404
Fragments arranged by size:
4404 1730 731
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2803 4220 844
Size: 1959 1417 3489
Fragments arranged by size:
3489 1959 1417
BsrBI CCG'CTC
Cuts at: 1394 1823 2666 2958 4752 1394
Size: 429 843 292 1794 3507
Fragments arranged by size:
3507 1794 843 429 292
BsrDI GCAATG_nn'
Cuts at: 65 1248 3672 5360 5534 65
Size: 1183 2424 1688 174 1396
Fragments arranged by size:
2424 1688 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2818 3552 4069 5509 5696 1700
Size: 110 1008 734 517 1440 187 2869
Fragments arranged by size:
2869 1440 1008 734 517 187 110
BsrGI T'GTAC_A
Cuts at: 96 2250 96
Size: 2154 4711
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6865
BssSI C'ACGA_G
Cuts at: 4925 5937 4925
Size: 1012 5853
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2969 794
Size: 2175 4690
BstBI TT'CG_AA
Cuts at: 5669 5669
Size: 6865
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6865
BstXI CCAn_nnnn'nTGG
Cuts at: 3604 3718 3604
Size: 114 6751
BstYI r'GATC_y
Cuts at: 1972 3995 4195 4562 4947 4964 6416 6502 6514
Size: 2023 200 367 385 17 1452 86 12
Cuts at: 6514 6860 1972
Size: 346 1977
Fragments arranged by size:
2023 1977 1452 385 367 346 200 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3762 4122 3762
Size: 360 6505
BtgI C'CryG_G
Cuts at: 513 1065 2004 3603 513
Size: 552 939 1599 3775
Fragments arranged by size:
3775 1599 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6126
Fragments arranged by size:
6126 683 56
BtsI GCAGTG_nn'
Cuts at: 2598 2994 4381 5175 5195 2598
Size: 396 1387 794 20 4268
Fragments arranged by size:
4268 1387 794 396 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6865
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3832 3867 529
Size: 35 3268 35 3527
Fragments arranged by size:
3527 3268 35 35
DraI TTT'AAA
Cuts at: 3358 4169 3358
Size: 811 6054
DraIII CAC_nnn'GTG
Cuts at: 3524 3524
Size: 6865
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5872 809
Size: 507 4556 1802
Fragments arranged by size:
4556 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2040 2226 2820
Size: 54 1425 50 275 18 210 186 594
Cuts at: 2820 3092 3229 3425 4056 4312 5201 6830 8
Size: 272 137 196 631 256 889 1629 43
Fragments arranged by size:
1629 1425 889 631 594 275 272 256
210 196 186 137 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2820 3425 4312 1537
Size: 275 1008 605 887 4090
Fragments arranged by size:
4090 1008 887 605 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3882 4795 1353
Size: 2529 913 3423
Fragments arranged by size:
3423 2529 913
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5452 5826 5972 387
Size: 1064 138 3863 374 146 1280
Fragments arranged by size:
3863 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4098 4098
Size: 6865
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2236 2346 2903 3229 3765 3900 4020
Size: 393 604 110 557 326 536 135 120
Cuts at: 4020 4928 5525 6312 6580 1239
Size: 908 597 787 268 1524
Fragments arranged by size:
1524 908 787 604 597 557 536 393
326 268 135 120 110
EcoO109I rG'GnC_Cy
Cuts at: 2471 2472 2558 2945 3077 3110 2471
Size: 1 86 387 132 33 6226
Fragments arranged by size:
6226 387 132 86 33 1
EcoRI G'AATT_C
Cuts at: 4174 4552 4174
Size: 378 6487
EcoRV GAT'ATC
Cuts at: 3889 3889
Size: 6865
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2048 2048
Size: 6865
FspI TGC'GCA
Cuts at: 5371 5371
Size: 6865
HaeII r_GCGC'y
Cuts at: 1678 2555 3559 6012 1678
Size: 877 1004 2453 2531
Fragments arranged by size:
2531 2453 1004 877
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2750 2782 4018 4050 4082
Size: 32 135 32 1178 32 1236 32 32
Cuts at: 4082 4114 5506 5538 5580 5612 1373
Size: 32 1392 32 42 32 2626
Fragments arranged by size:
2626 1392 1236 1178 135 42 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4209 4455 669
Size: 988 281 2271 246 3079
Fragments arranged by size:
3079 2271 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3407 748
Size: 2659 4206
HpaI GTT'AAC
Cuts at: 4455 4455
Size: 6865
KasI G'GCGC_C
Cuts at: 2551 2551
Size: 6865
KpnI G_GTAC'C
Cuts at: 4190 4190
Size: 6865
MfeI C'AATT_G
Cuts at: 3272 4464 3272
Size: 1192 5673
MluI A'CGCG_T
Cuts at: 6741 6741
Size: 6865
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5979 6163 1305
Size: 4674 184 2007
Fragments arranged by size:
4674 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4058 10
Size: 54 1425 343 2226 2817
Fragments arranged by size:
2817 2226 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2775 4823 5182 5341 518
Size: 648 1609 2048 359 159 2042
Fragments arranged by size:
2048 2042 1609 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2756 3167 3424 4114 4281 4958 6106 6351
Size: 1150 411 257 690 167 677 1148 245
Cuts at: 6351 1606
Size: 2120
Fragments arranged by size:
2120 1150 1148 690 677 411 257 245
167
NaeI GCC'GGC
Cuts at: 2820 3554 2820
Size: 734 6131
NarI GG'CG_CC
Cuts at: 2552 2552
Size: 6865
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5374
Fragments arranged by size:
5374 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6865
NgoMIV G'CCGG_C
Cuts at: 2818 3552 2818
Size: 734 6131
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6865
NotI GC'GGCC_GC
Cuts at: 3425 4312 3425
Size: 887 5978
NspI r_CATG'y
Cuts at: 1132 1229 4223 5768 1132
Size: 97 2994 1545 2229
Fragments arranged by size:
2994 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2586 2693 3597 1348
Size: 1238 107 904 4616
Fragments arranged by size:
4616 1238 904 107
PciI A'CATG_T
Cuts at: 5764 5764
Size: 6865
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2347 1108
Size: 1239 5626
PfoI T'CCnGG_A
Cuts at: 2738 2738
Size: 6865
PmeI GTTT'AAAC
Cuts at: 4169 4169
Size: 6865
PmlI CAC'GTG
Cuts at: 3985 3985
Size: 6865
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4934 4966 6447 6479 4934
Size: 32 1481 32 5320
Fragments arranged by size:
5320 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3077 3077
Size: 6865
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6865
PsiI TTA'TAA
Cuts at: 4435 4435
Size: 6865
PspOMI G'GGCC_C
Cuts at: 2471 2471
Size: 6865
PspXI vC'TCGA_Gb
Cuts at: 2661 2661
Size: 6865
PstI C_TGCA'G
Cuts at: 830 3109 4217 830
Size: 2279 1108 3478
Fragments arranged by size:
3478 2279 1108
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6865
PvuII CAG'CTG
Cuts at: 2756 4114 2756
Size: 1358 5507
SacI G_AGCT'C
Cuts at: 4100 4100
Size: 6865
SalI G'TCGA_C
Cuts at: 1936 4207 1936
Size: 2271 4594
SanDI GG'GwC_CC
Cuts at: 3077 3077
Size: 6865
SapI GCTCTTCn'nnn_
Cuts at: 3882 3882
Size: 6865
SbfI CC_TGCA'GG
Cuts at: 3109 4217 3109
Size: 1108 5757
ScaI AGT'ACT
Cuts at: 3814 5113 6825 3814
Size: 1299 1712 3854
Fragments arranged by size:
3854 1712 1299
SfcI C'TryA_G
Cuts at: 826 1045 3105 3990 4213 5348 6029 6220 826
Size: 219 2060 885 223 1135 681 191 1471
Fragments arranged by size:
2060 1471 1135 885 681 223 219 191
SfoI GGC'GCC
Cuts at: 2553 2553
Size: 6865
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6865
SgrAI Cr'CCGG_yG
Cuts at: 1700 4069 1700
Size: 2369 4496
SmaI CCC'GGG
Cuts at: 1872 2549 2641 2802 4192 1872
Size: 677 92 161 1390 4545
Fragments arranged by size:
4545 1390 677 161 92
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2661 4157 4969 5870 6132 820
Size: 197 926 718 1496 812 901 262 1553
Fragments arranged by size:
1553 1496 926 901 812 718 262 197
SnaBI TAC'GTA
Cuts at: 493 4656 493
Size: 4163 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6865
SphI G_CATG'C
Cuts at: 1132 4223 1132
Size: 3091 3774
SspI AAT'ATT
Cuts at: 5 52 4789 5
Size: 47 4737 2081
Fragments arranged by size:
4737 2081 47
StuI AGG'CCT
Cuts at: 1517 4009 1517
Size: 2492 4373
StyI C'CwwG_G
Cuts at: 513 1065 2004 4304 513
Size: 552 939 2300 3074
Fragments arranged by size:
3074 2300 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2471 3218 4892 4909 5062 5247 5692
Size: 1255 891 747 1674 17 153 185 445
Cuts at: 5692 325
Size: 1498
Fragments arranged by size:
1674 1498 1255 891 747 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2250 3812 5111
Size: 274 80 33 51 1443 273 1562 1299
Cuts at: 5111 6823 96
Size: 1712 138
Fragments arranged by size:
1712 1562 1443 1299 274 273 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6825 6857 6825
Size: 32 6833
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6865
XbaI T'CTAG_A
Cuts at: 4201 4201
Size: 6865
XhoI C'TCGA_G
Cuts at: 1943 2661 1943
Size: 718 6147
XmaI C'CCGG_G
Cuts at: 1870 2547 2639 2800 4190 1870
Size: 677 92 161 1390 4545
Fragments arranged by size:
4545 1390 677 161 92
XmnI GAAnn'nnTTC
Cuts at: 2028 4994 2028
Size: 2966 3899
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4673 276
Size: 53 83 186 936 3139 2468
Fragments arranged by size:
3139 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BfrBI BsiWI BstZ17I
EcoNI FseI FspAI NruI NsiI PacI PsrI RsrII
SacII SexAI SfiI SrfI SwaI XcmI