Restriction map for
pFN21AB8718
(Circular) (Six-base) MAPSORT of: pFN21AB8718.seq Check: 1381 from: 1 to: 6520
pFN21AB8718 6520 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2600 2600
Size: 6520
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3270 3606 4330 278
Size: 53 83 186 936 1734 336 724 2468
Fragments arranged by size:
2468 1734 936 724 336 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2455 3863 1937
Size: 518 1408 4594
Fragments arranged by size:
4594 1408 518
Acc65I G'GTAC_C
Cuts at: 3841 3841
Size: 6520
AclI AA'CG_TT
Cuts at: 1563 4647 5020 1563
Size: 3084 373 3063
Fragments arranged by size:
3084 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3091 3245 3260 3653 4583 5967 6235 1632
Size: 1459 154 15 393 930 1384 268 1917
Fragments arranged by size:
1917 1459 1384 930 393 268 154 15
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6323
AflIII A'CryG_T
Cuts at: 1203 2943 3501 3792 5419 6396 1203
Size: 1740 558 291 1627 977 1327
Fragments arranged by size:
1740 1627 1327 977 558 291
AgeI A'CCGG_T
Cuts at: 5351 5351
Size: 6520
AhdI GACnn_n'nnGTC
Cuts at: 5249 5249
Size: 6520
AleI CACnn'nnGTG
Cuts at: 1166 2591 1166
Size: 1425 5095
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2609 2810 2900 3272 3584 1184
Size: 1425 201 90 372 312 4120
Fragments arranged by size:
4120 1425 372 312 201 90
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2930 2962 2930
Size: 32 6488
AlwNI CAG_nnn'CTG
Cuts at: 4263 5835 4263
Size: 1572 4948
ApaLI G'TGCA_C
Cuts at: 2677 4577 5733 2677
Size: 1900 1156 3464
Fragments arranged by size:
3464 1900 1156
ApoI r'AATT_y
Cuts at: 1493 3829 4055 4207 6316 1493
Size: 2336 226 152 2109 1697
Fragments arranged by size:
2336 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5074 160
Size: 872 4042 1606
Fragments arranged by size:
4042 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6520
AvaI C'yCGr_G
Cuts at: 1870 1943 2115 2237 2994 3339 3845 1870
Size: 73 172 122 757 345 506 4545
Fragments arranged by size:
4545 757 506 345 172 122 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2566 2599 2566
Size: 33 6487
BamHI G'GATC_C
Cuts at: 3815 3850 4217 3815
Size: 35 367 6118
Fragments arranged by size:
6118 367 35
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2399 2691 2890 3841 5296
Size: 325 217 627 612 292 199 951 1455
Cuts at: 5296 618
Size: 1842
Fragments arranged by size:
1842 1455 951 627 612 325 292 217
199
BanII G_rGCy'C
Cuts at: 1391 2043 3839 1391
Size: 652 1796 4072
Fragments arranged by size:
4072 1796 652
BbeI G_GCGC'C
Cuts at: 2403 2403
Size: 6520
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5549
BbvCI CC'TCA_GC
Cuts at: 3717 3717
Size: 6520
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2992 3026 3235 3269 4711 4745 1903
Size: 34 1055 34 209 34 1442 34 3678
Fragments arranged by size:
3678 1442 1055 209 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4412 5622 4412
Size: 1210 5310
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6520
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5131 136
Size: 107 122 71 631 605 3459 1525
Fragments arranged by size:
3459 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6515 6515
Size: 6520
BlpI GC'TnA_GC
Cuts at: 2717 3937 2717
Size: 1220 5300
Bme1580I G_kGCm'C
Cuts at: 1790 2681 3142 3297 3537 4581 5737 1790
Size: 891 461 155 240 1044 1156 2573
Fragments arranged by size:
2573 1156 1044 891 461 240 155
BmgBI CAC'GTC
Cuts at: 1318 2227 2827 1318
Size: 909 600 5011
Fragments arranged by size:
5011 909 600
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2430 4251 5209
Size: 416 53 83 399 24 1013 1821 958
Cuts at: 5209 442
Size: 1753
Fragments arranged by size:
1821 1753 1013 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6520
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2674 2674
Size: 6520
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2450 2888 3113 3448 3644 5180 1239
Size: 1211 438 225 335 196 1536 2579
Fragments arranged by size:
2579 1536 1211 438 335 225 196
Bpu10I CC'TnA_GC
Cuts at: 2742 2808 3477 3717 2742
Size: 66 669 240 5545
Fragments arranged by size:
5545 669 240 66
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2027 4645 5510 5808 2027
Size: 2618 865 298 2739
Fragments arranged by size:
2739 2618 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5183 882
Size: 617 3684 2219
Fragments arranged by size:
3684 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4311 493
Size: 711 3107 2702
Fragments arranged by size:
3107 2702 711
BsaBI GATnn'nnATC
Cuts at: 2973 6514 2973
Size: 3541 2979
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2400 2960 3101 3267
Size: 53 83 186 936 867 560 141 166
Cuts at: 3267 3603 4327 4709 5344 275
Size: 336 724 382 635 1451
Fragments arranged by size:
1451 936 867 724 635 560 382 336
186 166 141 83 53
BsaWI w'CCGG_w
Cuts at: 2664 2955 3744 4953 5351 5625 5772 6183 2664
Size: 291 789 1209 398 274 147 411 3001
Fragments arranged by size:
3001 1209 789 411 398 291 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2075 2105 2153 2183 2477
Size: 30 165 30 672 30 48 30 294
Cuts at: 2477 2507 2930 2960 3620 3650 1178
Size: 30 423 30 660 30 4048
Fragments arranged by size:
4048 672 660 423 294 165 48 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2099 2174 3110 3641 1186
Size: 913 75 936 531 4065
Fragments arranged by size:
4065 936 913 531 75
BseYI C'CCAG_C
Cuts at: 2189 2266 2271 2570 5723 6219 2189
Size: 77 5 299 3153 496 2490
Fragments arranged by size:
3153 2490 496 299 77 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2628 2634 2652 3309 3758 1163
Size: 573 892 6 18 657 449 3925
Fragments arranged by size:
3925 892 657 573 449 18 6
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2665 2707 3970 4731 5759 1540
Size: 275 186 664 42 1263 761 1028 2301
Fragments arranged by size:
2301 1263 1028 761 664 275 186 42
BsiHKAI G_wGCw'C
Cuts at: 2043 2598 2681 2799 3530 4581 4666 5737 2043
Size: 555 83 118 731 1051 85 1071 2826
Fragments arranged by size:
2826 1071 1051 731 555 118 85 83
BsmI GAATG_Cn'
Cuts at: 4031 4124 4031
Size: 93 6427
BsmBI CGTCTCn'nnnn_
Cuts at: 3092 3092
Size: 6520
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2043 2598 2681 2799 3142 3297 3530
Size: 399 253 555 83 118 343 155 233
Cuts at: 3530 3537 3839 4581 4666 5737 1391
Size: 7 302 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 555 399 343 302 253
233 155 118 85 83 7
BspEI T'CCGG_A
Cuts at: 2955 3744 6183 2955
Size: 789 2439 3292
Fragments arranged by size:
3292 2439 789
BspHI T'CATG_A
Cuts at: 3144 4409 6139 3144
Size: 1265 1730 3525
Fragments arranged by size:
3525 1730 1265
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2600 3875 844
Size: 1756 1275 3489
Fragments arranged by size:
3489 1756 1275
BsrBI CCG'CTC
Cuts at: 1394 1823 4407 1394
Size: 429 2584 3507
Fragments arranged by size:
3507 2584 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2137 5015 5189 65
Size: 1183 889 2878 174 1396
Fragments arranged by size:
2878 1396 1183 889 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2688 3023 5164 5351 1700
Size: 110 878 335 2141 187 2869
Fragments arranged by size:
2869 2141 878 335 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6520
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6520
BssSI C'ACGA_G
Cuts at: 4580 5592 4580
Size: 1012 5508
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6520
BstBI TT'CG_AA
Cuts at: 5324 5324
Size: 6520
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6520
BstXI CCAn_nnnn'nTGG
Cuts at: 2035 2132 2545 2980 2035
Size: 97 413 435 5575
Fragments arranged by size:
5575 435 413 97
BstYI r'GATC_y
Cuts at: 1972 3619 3815 3850 4217 4602 4619 6071 6157
Size: 1647 196 35 367 385 17 1452 86
Cuts at: 6157 6169 6515 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1647 1452 385 367 346 196 86
35 17 12
Bsu36I CC'TnA_GG
Cuts at: 2196 2756 3311 3730 2196
Size: 560 555 419 4986
Fragments arranged by size:
4986 560 555 419
BtgI C'CryG_G
Cuts at: 513 1065 2004 2371 3675 513
Size: 552 939 367 1304 3358
Fragments arranged by size:
3358 1304 939 552 367
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3417 487
Size: 683 56 2191 3590
Fragments arranged by size:
3590 2191 683 56
BtsI GCAGTG_nn'
Cuts at: 2639 3297 4036 4830 4850 2639
Size: 658 739 794 20 4309
Fragments arranged by size:
4309 794 739 658 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6520
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6485
DraI TTT'AAA
Cuts at: 3824 3824
Size: 6520
DraIII CAC_nnn'GTG
Cuts at: 3076 3076
Size: 6520
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2669 3193 5527 809
Size: 507 1353 524 2334 1802
Fragments arranged by size:
2334 1802 1353 524 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2094 2245 2547
Size: 54 1425 50 275 18 264 151 302
Cuts at: 2547 3045 3678 3967 4856 6485 8
Size: 498 633 289 889 1629 43
Fragments arranged by size:
1629 1425 889 633 498 302 289 275
264 151 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3967 1537
Size: 275 2155 4090
Fragments arranged by size:
4090 2155 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3236 4450 1353
Size: 1883 1214 3423
Fragments arranged by size:
3423 1883 1214
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3533 5107 5481 5627 387
Size: 1064 138 1944 1574 374 146 1280
Fragments arranged by size:
1944 1574 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2041 2041
Size: 6520
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2450 2888 3091 3113 3245 3260 3448
Size: 393 818 438 203 22 132 15 188
Cuts at: 3448 3644 3653 4583 5180 5967 6235 1239
Size: 196 9 930 597 787 268 1524
Fragments arranged by size:
1524 930 818 787 597 438 393 268
203 196 188 132 22 15 9
EcoNI CCTnn'n_nnAGG
Cuts at: 3109 3109
Size: 6520
EcoO109I rG'GnC_Cy
Cuts at: 2233 2425 2515 2233
Size: 192 90 6238
Fragments arranged by size:
6238 192 90
EcoRI G'AATT_C
Cuts at: 3829 4207 3829
Size: 378 6142
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2875 2875
Size: 6520
FspI TGC'GCA
Cuts at: 5026 5026
Size: 6520
HaeII r_GCGC'y
Cuts at: 1678 2167 2403 5667 1678
Size: 489 236 3264 2531
Fragments arranged by size:
3264 2531 489 236
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2075 2107 2153 2185 3333
Size: 32 135 32 503 32 46 32 1148
Cuts at: 3333 3365 5161 5193 5235 5267 1373
Size: 32 1796 32 42 32 2626
Fragments arranged by size:
2626 1796 1148 503 135 46 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3358 3864 4110 669
Size: 988 281 1420 506 246 3079
Fragments arranged by size:
3079 1420 988 506 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6520
HpaI GTT'AAC
Cuts at: 4110 4110
Size: 6520
KasI G'GCGC_C
Cuts at: 2399 2399
Size: 6520
KpnI G_GTAC'C
Cuts at: 3845 3845
Size: 6520
MfeI C'AATT_G
Cuts at: 4119 4119
Size: 6520
MluI A'CGCG_T
Cuts at: 6396 6396
Size: 6520
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2685 5634 5818 1305
Size: 1380 2949 184 2007
Fragments arranged by size:
2949 2007 1380 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2247 2549 3680 10
Size: 54 1425 343 415 302 1131 2850
Fragments arranged by size:
2850 1425 1131 415 343 302 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2591 2792 3149 4478 4837 4996 518
Size: 648 1425 201 357 1329 359 159 2042
Fragments arranged by size:
2042 1425 1329 648 359 357 201 159
MspA1I CmG'CkG
Cuts at: 1606 2524 2618 2749 3398 3936 4613 5761 6006
Size: 918 94 131 649 538 677 1148 245
Cuts at: 6006 1606
Size: 2120
Fragments arranged by size:
2120 1148 918 677 649 538 245 131
94
NaeI GCC'GGC
Cuts at: 3025 3025
Size: 6520
NarI GG'CG_CC
Cuts at: 2400 2400
Size: 6520
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5029
Fragments arranged by size:
5029 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6520
NgoMIV G'CCGG_C
Cuts at: 3023 3023
Size: 6520
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6520
NotI GC'GGCC_GC
Cuts at: 3967 3967
Size: 6520
NspI r_CATG'y
Cuts at: 1132 1229 3184 3505 3796 3878 5423 1132
Size: 97 1955 321 291 82 1545 2229
Fragments arranged by size:
2229 1955 1545 321 291 97 82
PasI CC'CwG_GG
Cuts at: 1348 2317 1348
Size: 969 5551
PciI A'CATG_T
Cuts at: 3501 3792 5419 3501
Size: 291 1627 4602
Fragments arranged by size:
4602 1627 291
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2317 2570 1108
Size: 1209 253 5058
Fragments arranged by size:
5058 1209 253
PmeI GTTT'AAAC
Cuts at: 3824 3824
Size: 6520
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2930 2962 4589 4621 6102 6134 2930
Size: 32 1627 32 1481 32 3316
Fragments arranged by size:
3316 1627 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2233 2425 2233
Size: 192 6328
PshAI GACnn'nnGTC
Cuts at: 1613 3377 1613
Size: 1764 4756
PsiI TTA'TAA
Cuts at: 4090 4090
Size: 6520
PstI C_TGCA'G
Cuts at: 830 2529 3872 830
Size: 1699 1343 3478
Fragments arranged by size:
3478 1699 1343
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6520
PvuII CAG'CTG
Cuts at: 2524 2618 2749 3398 2524
Size: 94 131 649 5646
Fragments arranged by size:
5646 649 131 94
SacI G_AGCT'C
Cuts at: 2043 2043
Size: 6520
SalI G'TCGA_C
Cuts at: 1936 3862 1936
Size: 1926 4594
SapI GCTCTTCn'nnn_
Cuts at: 3236 3236
Size: 6520
SbfI CC_TGCA'GG
Cuts at: 3872 3872
Size: 6520
ScaI AGT'ACT
Cuts at: 4768 6480 4768
Size: 1712 4808
SfcI C'TryA_G
Cuts at: 826 1045 2519 2525 3868 5003 5684 5875 826
Size: 219 1474 6 1343 1135 681 191 1471
Fragments arranged by size:
1474 1471 1343 1135 681 219 191 6
SfoI GGC'GCC
Cuts at: 2401 2401
Size: 6520
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6520
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6520
SmaI CCC'GGG
Cuts at: 1872 2117 3847 1872
Size: 245 1730 4545
Fragments arranged by size:
4545 1730 245
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2042 2237 4624 5525 5787 820
Size: 197 926 99 195 2387 901 262 1553
Fragments arranged by size:
2387 1553 926 901 262 197 195 99
SnaBI TAC'GTA
Cuts at: 493 4311 493
Size: 3818 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6520
SphI G_CATG'C
Cuts at: 1132 3878 1132
Size: 2746 3774
SspI AAT'ATT
Cuts at: 5 52 4444 5
Size: 47 4392 2081
Fragments arranged by size:
4392 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6520
StyI C'CwwG_G
Cuts at: 513 1065 2004 2538 2763 3708 3959 513
Size: 552 939 534 225 945 251 3074
Fragments arranged by size:
3074 945 939 552 534 251 225
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2594 4547 4564 4717 4902 5347 325
Size: 1255 1014 1953 17 153 185 445 1498
Fragments arranged by size:
1953 1498 1255 1014 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2871 4766 6478
Size: 274 80 33 51 1443 894 1895 1712
Cuts at: 6478 96
Size: 138
Fragments arranged by size:
1895 1712 1443 894 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6480 6512 6480
Size: 32 6488
Tth111I GACn'n_nGTC
Cuts at: 1316 3602 1316
Size: 2286 4234
XbaI T'CTAG_A
Cuts at: 3856 3856
Size: 6520
XhoI C'TCGA_G
Cuts at: 1943 2237 1943
Size: 294 6226
XmaI C'CCGG_G
Cuts at: 1870 2115 3845 1870
Size: 245 1730 4545
Fragments arranged by size:
4545 1730 245
XmnI GAAnn'nnTTC
Cuts at: 2945 4649 2945
Size: 1704 4816
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3268 3604 4328 276
Size: 53 83 186 936 1734 336 724 2468
Fragments arranged by size:
2468 1734 936 724 336 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BfrBI BsiWI BstZ17I EcoRV
FseI FspAI NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI
SwaI XcmI