Restriction map for
pFN21AB8715
(Circular) (Six-base) MAPSORT of: pFN21AB8715.seq Check: 9956 from: 1 to: 5863
pFN21AB8715 5863 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2140 2575 2140
Size: 435 5428
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3673 278
Size: 53 83 186 936 2137 2468
Fragments arranged by size:
2468 2137 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3206 1937
Size: 1269 4594
Acc65I G'GTAC_C
Cuts at: 3184 3184
Size: 5863
AclI AA'CG_TT
Cuts at: 1563 3990 4363 1563
Size: 2427 373 3063
Fragments arranged by size:
3063 2427 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3926 5310 5578 1632
Size: 2294 1384 268 1917
Fragments arranged by size:
2294 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5666
AflIII A'CryG_T
Cuts at: 1203 4762 5739 1203
Size: 3559 977 1327
Fragments arranged by size:
3559 1327 977
AgeI A'CCGG_T
Cuts at: 4694 4694
Size: 5863
AhdI GACnn_n'nnGTC
Cuts at: 2743 4592 2743
Size: 1849 4014
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5863
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5863
AlwNI CAG_nnn'CTG
Cuts at: 2590 2893 2998 3606 5178 2590
Size: 303 105 608 1572 3275
Fragments arranged by size:
3275 1572 608 303 105
ApaI G_GGCC'C
Cuts at: 2021 2021
Size: 5863
ApaLI G'TGCA_C
Cuts at: 2153 3920 5076 2153
Size: 1767 1156 2940
Fragments arranged by size:
2940 1767 1156
ApoI r'AATT_y
Cuts at: 1493 3172 3398 3550 5659 1493
Size: 1679 226 152 2109 1697
Fragments arranged by size:
2109 1697 1679 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4417 160
Size: 872 3385 1606
Fragments arranged by size:
3385 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5863
AvaI C'yCGr_G
Cuts at: 1870 1943 2653 3188 1870
Size: 73 710 535 4545
Fragments arranged by size:
4545 710 535 73
BamHI G'GATC_C
Cuts at: 3193 3560 3193
Size: 367 5496
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3065 3184 4639 618
Size: 325 217 627 1278 119 1455 1842
Fragments arranged by size:
1842 1455 1278 627 325 217 119
BanII G_rGCy'C
Cuts at: 1391 2021 2813 3182 1391
Size: 630 792 369 4072
Fragments arranged by size:
4072 792 630 369
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2763 928
Size: 971 864 4028
Fragments arranged by size:
4028 971 864
BbvCI CC'TCA_GC
Cuts at: 2281 2281
Size: 5863
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4054 4088 1903
Size: 34 50 34 2033 34 3678
Fragments arranged by size:
3678 2033 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2357 3755 4965 2357
Size: 1398 1210 3255
Fragments arranged by size:
3255 1398 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5863
BfrBI ATG'CAT
Cuts at: 2008 2008
Size: 5863
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2025 2256 2390
Size: 107 122 71 631 605 353 231 134
Cuts at: 2390 2440 2531 2638 4474 136
Size: 50 91 107 1836 1525
Fragments arranged by size:
1836 1525 631 605 353 231 134 122
107 107 91 71 50
BglII A'GATC_T
Cuts at: 5858 5858
Size: 5863
BlpI GC'TnA_GC
Cuts at: 2480 3280 2480
Size: 800 5063
Bme1580I G_kGCm'C
Cuts at: 1790 2021 2157 2261 2431 3101 3924 5080 1790
Size: 231 136 104 170 670 823 1156 2573
Fragments arranged by size:
2573 1156 823 670 231 170 136 104
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5863
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2761 2842 3594
Size: 416 53 83 399 24 1344 81 752
Cuts at: 3594 4552 442
Size: 958 1753
Fragments arranged by size:
1753 1344 958 752 416 399 83 81
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5863
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2830 2830
Size: 5863
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3020 4523 1239
Size: 1781 1503 2579
Fragments arranged by size:
2579 1781 1503
Bpu10I CC'TnA_GC
Cuts at: 2207 2281 2207
Size: 74 5789
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3988 4853 5151 3988
Size: 865 298 4700
Fragments arranged by size:
4700 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3098 4526 882
Size: 617 1599 1428 2219
Fragments arranged by size:
2219 1599 1428 617
BsaAI yAC'GTr
Cuts at: 493 1204 3654 493
Size: 711 2450 2702
Fragments arranged by size:
2702 2450 711
BsaBI GATnn'nnATC
Cuts at: 5857 5857
Size: 5863
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2601 3670 4052 4687
Size: 53 83 186 936 1068 1069 382 635
Cuts at: 4687 275
Size: 1451
Fragments arranged by size:
1451 1069 1068 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2553 4296 4694 4968 5115 5526 2553
Size: 1743 398 274 147 411 2890
Fragments arranged by size:
2890 1743 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3078 3108 1178
Size: 30 165 30 1675 30 3933
Fragments arranged by size:
3933 1675 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2047 2803 2968 1186
Size: 861 756 165 4081
Fragments arranged by size:
4081 861 756 165
BseYI C'CCAG_C
Cuts at: 2038 2393 2543 2837 5066 5562 2038
Size: 355 150 294 2229 496 2339
Fragments arranged by size:
2339 2229 496 355 294 150
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2367 2629 2813 1163
Size: 573 631 262 184 4213
Fragments arranged by size:
4213 631 573 262 184
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3313 4074 5102 1540
Size: 275 186 1312 761 1028 2301
Fragments arranged by size:
2301 1312 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2084 2157 3924 4009 5080 2084
Size: 73 1767 85 1071 2867
Fragments arranged by size:
2867 1767 1071 85 73
BsmI GAATG_Cn'
Cuts at: 3374 3467 3374
Size: 93 5770
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2021 2084 2157 2261 2431 2813 3101
Size: 399 231 63 73 104 170 382 288
Cuts at: 3101 3182 3924 4009 5080 1391
Size: 81 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 382 288 231 170
104 85 81 73 63
BspEI T'CCGG_A
Cuts at: 5526 5526
Size: 5863
BspHI T'CATG_A
Cuts at: 3752 5482 3752
Size: 1730 4133
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2140 2575 3218 844
Size: 1296 435 643 3489
Fragments arranged by size:
3489 1296 643 435
BsrBI CCG'CTC
Cuts at: 1394 1823 3750 1394
Size: 429 1927 3507
Fragments arranged by size:
3507 1927 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4358 4532 65
Size: 1183 3110 174 1396
Fragments arranged by size:
3110 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2102 2157 2351 2525 4507 4694 1700
Size: 110 292 55 194 174 1982 187 2869
Fragments arranged by size:
2869 1982 292 194 187 174 110 55
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5863
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5863
BssSI C'ACGA_G
Cuts at: 3923 4935 3923
Size: 1012 4851
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2161 2590 2893 794
Size: 1367 429 303 3764
Fragments arranged by size:
3764 1367 429 303
BstBI TT'CG_AA
Cuts at: 4667 4667
Size: 5863
BstEII G'GTnAC_C
Cuts at: 1184 3112 1184
Size: 1928 3935
BstYI r'GATC_y
Cuts at: 1972 3193 3560 3945 3962 5414 5500 5512 5858
Size: 1221 367 385 17 1452 86 12 346
Cuts at: 5858 1972
Size: 1977
Fragments arranged by size:
1977 1452 1221 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2636 2783 2636
Size: 147 5716
BtgI C'CryG_G
Cuts at: 513 1065 2023 2422 2438 2450 2954 3118 513
Size: 552 958 399 16 12 504 164 3258
Fragments arranged by size:
3258 958 552 504 399 164 16 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5124
Fragments arranged by size:
5124 683 56
BtsI GCAGTG_nn'
Cuts at: 2042 2322 2429 2474 3379 4173 4193 2042
Size: 280 107 45 905 794 20 3712
Fragments arranged by size:
3712 905 794 280 107 45 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5863
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5828
DraI TTT'AAA
Cuts at: 3167 3167
Size: 5863
DraIII CAC_nnn'GTG
Cuts at: 2088 2088
Size: 5863
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4870 809
Size: 507 3554 1802
Fragments arranged by size:
3554 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2026 2382 3310
Size: 54 1425 50 275 18 196 356 928
Cuts at: 3310 4199 5828 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 928 889 356 275 196 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3310 1537
Size: 275 1498 4090
Fragments arranged by size:
4090 1498 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3793 1353
Size: 2440 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2681 4450 4824 4970 387
Size: 1064 138 1092 1769 374 146 1280
Fragments arranged by size:
1769 1280 1092 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3020 3926 4523 5310 5578 1239
Size: 393 1388 906 597 787 268 1524
Fragments arranged by size:
1524 1388 906 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2017 2018 2547 2613 3149 2017
Size: 1 529 66 536 4731
Fragments arranged by size:
4731 536 529 66 1
EcoRI G'AATT_C
Cuts at: 3172 3550 3172
Size: 378 5485
FseI GG_CCGG'CC
Cuts at: 2106 2106
Size: 5863
FspI TGC'GCA
Cuts at: 4369 4369
Size: 5863
HaeII r_GCGC'y
Cuts at: 1678 5010 1678
Size: 3332 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2729 2761 4504 4536 4578
Size: 32 135 32 1157 32 1743 32 42
Cuts at: 4578 4610 1373
Size: 32 2626
Fragments arranged by size:
2626 1743 1157 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3207 3453 669
Size: 988 281 1269 246 3079
Fragments arranged by size:
3079 1269 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5863
HpaI GTT'AAC
Cuts at: 3453 3453
Size: 5863
KpnI G_GTAC'C
Cuts at: 3188 3188
Size: 5863
MfeI C'AATT_G
Cuts at: 3462 3462
Size: 5863
MluI A'CGCG_T
Cuts at: 5739 5739
Size: 5863
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3064 4977 5161 1305
Size: 1759 1913 184 2007
Fragments arranged by size:
2007 1913 1759 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2028 2384 10
Size: 54 1425 343 196 356 3489
Fragments arranged by size:
3489 1425 356 343 196 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3821 4180 4339 518
Size: 648 2655 359 159 2042
Fragments arranged by size:
2655 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2358 2543 2998 3279 3956 5104 5349 1606
Size: 752 185 455 281 677 1148 245 2120
Fragments arranged by size:
2120 1148 752 677 455 281 245 185
NaeI GCC'GGC
Cuts at: 2104 2353 2527 2104
Size: 249 174 5440
Fragments arranged by size:
5440 249 174
NcoI C'CATG_G
Cuts at: 513 1065 2023 2954 3118 513
Size: 552 958 931 164 3258
Fragments arranged by size:
3258 958 931 552 164
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5863
NgoMIV G'CCGG_C
Cuts at: 2102 2351 2525 2102
Size: 249 174 5440
Fragments arranged by size:
5440 249 174
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5863
NotI GC'GGCC_GC
Cuts at: 3310 3310
Size: 5863
NsiI A_TGCA'T
Cuts at: 2010 2010
Size: 5863
NspI r_CATG'y
Cuts at: 1132 1229 2012 2279 2371 3221 4766 1132
Size: 97 783 267 92 850 1545 2229
Fragments arranged by size:
2229 1545 850 783 267 97 92
PasI CC'CwG_GG
Cuts at: 1348 2176 2855 1348
Size: 828 679 4356
Fragments arranged by size:
4356 828 679
PciI A'CATG_T
Cuts at: 4762 4762
Size: 5863
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2176 1108
Size: 1068 4795
PmeI GTTT'AAAC
Cuts at: 3167 3167
Size: 5863
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3932 3964 5445 5477 3932
Size: 32 1481 32 4318
Fragments arranged by size:
4318 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2613 3149 2613
Size: 536 5327
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5863
PsiI TTA'TAA
Cuts at: 3433 3433
Size: 5863
PspOMI G'GGCC_C
Cuts at: 2017 2017
Size: 5863
PstI C_TGCA'G
Cuts at: 830 2505 2586 2595 2898 2994 3215 830
Size: 1675 81 9 303 96 221 3478
Fragments arranged by size:
3478 1675 303 221 96 81 9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5863
PvuII CAG'CTG
Cuts at: 2358 2543 2998 2358
Size: 185 455 5223
Fragments arranged by size:
5223 455 185
SalI G'TCGA_C
Cuts at: 1936 3205 1936
Size: 1269 4594
SbfI CC_TGCA'GG
Cuts at: 3215 3215
Size: 5863
ScaI AGT'ACT
Cuts at: 4111 5823 4111
Size: 1712 4151
SfcI C'TryA_G
Cuts at: 826 1045 2501 2582 2591 2894 2990 3211 4346
Size: 219 1456 81 9 303 96 221 1135
Cuts at: 4346 5027 5218 826
Size: 681 191 1471
Fragments arranged by size:
1471 1456 1135 681 303 221 219 191
96 81 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 2025 2025
Size: 5863
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5863
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5863
SmaI CCC'GGG
Cuts at: 1872 2655 3190 1872
Size: 783 535 4545
Fragments arranged by size:
4545 783 535
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3967 4868 5130 820
Size: 197 926 2024 901 262 1553
Fragments arranged by size:
2024 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3654 493
Size: 3161 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5863
SphI G_CATG'C
Cuts at: 1132 2012 2279 3221 1132
Size: 880 267 942 3774
Fragments arranged by size:
3774 942 880 267
SspI AAT'ATT
Cuts at: 5 52 3787 5
Size: 47 3735 2081
Fragments arranged by size:
3735 2081 47
StuI AGG'CCT
Cuts at: 1517 2199 2331 2701 2788 2826 2912 1517
Size: 682 132 370 87 38 86 4468
Fragments arranged by size:
4468 682 370 132 87 86 38
StyI C'CwwG_G
Cuts at: 513 1065 2023 2332 2616 2877 2954 3118 3302
Size: 552 958 309 284 261 77 164 184
Cuts at: 3302 513
Size: 3074
Fragments arranged by size:
3074 958 552 309 284 261 184 164
77
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2696 3890 3907 4060 4245 4690 325
Size: 1255 1116 1194 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1194 1116 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4109 5821 96
Size: 274 80 33 51 1443 2132 1712 138
Fragments arranged by size:
2132 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3078 3110 5823 5855 3078
Size: 32 2713 32 3086
Fragments arranged by size:
3086 2713 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5863
XbaI T'CTAG_A
Cuts at: 3199 3199
Size: 5863
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3055 3125 3055
Size: 70 5793
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5863
XmaI C'CCGG_G
Cuts at: 1870 2653 3188 1870
Size: 783 535 4545
Fragments arranged by size:
4545 783 535
XmnI GAAnn'nnTTC
Cuts at: 3992 3992
Size: 5863
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3671 276
Size: 53 83 186 936 2137 2468
Fragments arranged by size:
2468 2137 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SbfI ScaI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BsiWI BsmBI
BstXI BstZ17I EcoICRI EcoNI EcoRV FalI FspAI KasI
NarI NruI PacI PfoI PmlI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfoI SrfI SwaI