Restriction map for
pFN21AB8710
(Circular) (Six-base) MAPSORT of: pFN21AB8710.seq Check: 2543 from: 1 to: 5905
pFN21AB8710 5905 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3715 278
Size: 53 83 186 936 2179 2468
Fragments arranged by size:
2468 2179 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3248 1937
Size: 1311 4594
Acc65I G'GTAC_C
Cuts at: 3226 3226
Size: 5905
AclI AA'CG_TT
Cuts at: 1563 4032 4405 1563
Size: 2469 373 3063
Fragments arranged by size:
3063 2469 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3968 5352 5620 1632
Size: 2336 1384 268 1917
Fragments arranged by size:
2336 1917 1384 268
AfeI AGC'GCT
Cuts at: 2429 2429
Size: 5905
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5708
AflIII A'CryG_T
Cuts at: 1203 2918 4804 5781 1203
Size: 1715 1886 977 1327
Fragments arranged by size:
1886 1715 1327 977
AgeI A'CCGG_T
Cuts at: 4736 4736
Size: 5905
AhdI GACnn_n'nnGTC
Cuts at: 4634 4634
Size: 5905
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5905
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5905
AlwNI CAG_nnn'CTG
Cuts at: 3648 5220 3648
Size: 1572 4333
ApaI G_GGCC'C
Cuts at: 2095 2095
Size: 5905
ApaLI G'TGCA_C
Cuts at: 3962 5118 3962
Size: 1156 4749
ApoI r'AATT_y
Cuts at: 1493 2514 2538 2827 2853 3031 3214 3440 3592
Size: 1021 24 289 26 178 183 226 152
Cuts at: 3592 5701 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1021 289 226 183 178 152
26 24
AseI AT'TA_AT
Cuts at: 160 1032 4459 160
Size: 872 3427 1606
Fragments arranged by size:
3427 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5905
AvaI C'yCGr_G
Cuts at: 1870 1943 2087 2094 2108 2264 2300 3230 1870
Size: 73 144 7 14 156 36 930 4545
Fragments arranged by size:
4545 930 156 144 73 36 14 7
AvrII C'CTAG_G
Cuts at: 2953 2953
Size: 5905
BamHI G'GATC_C
Cuts at: 3019 3235 3602 3019
Size: 216 367 5322
Fragments arranged by size:
5322 367 216
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2248 2567 3226 4681 618
Size: 325 217 627 461 319 659 1455 1842
Fragments arranged by size:
1842 1455 659 627 461 325 319 217
BanII G_rGCy'C
Cuts at: 1391 2041 2095 2107 2307 3224 1391
Size: 650 54 12 200 917 4072
Fragments arranged by size:
4072 917 650 200 54 12
BbeI G_GCGC'C
Cuts at: 2252 2252
Size: 5905
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4934
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4096 4130 1903
Size: 34 2159 34 3678
Fragments arranged by size:
3678 2159 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3797 5007 3797
Size: 1210 4695
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5905
BfrBI ATG'CAT
Cuts at: 2889 2889
Size: 5905
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2196 2235 2342
Size: 107 122 71 631 605 524 39 107
Cuts at: 2342 4516 136
Size: 2174 1525
Fragments arranged by size:
2174 1525 631 605 524 122 107 107
71 39
BglII A'GATC_T
Cuts at: 5900 5900
Size: 5905
BlpI GC'TnA_GC
Cuts at: 3322 3322
Size: 5905
Bme1580I G_kGCm'C
Cuts at: 1790 2088 2095 2301 3966 5122 1790
Size: 298 7 206 1665 1156 2573
Fragments arranged by size:
2573 1665 1156 298 206 7
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5905
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3636 4594 442
Size: 416 53 83 399 24 2219 958 1753
Fragments arranged by size:
2219 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5905
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2134 3198 2134
Size: 1064 4841
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2982 4565 1239
Size: 1743 1583 2579
Fragments arranged by size:
2579 1743 1583
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4030 4895 5193 4030
Size: 865 298 4742
Fragments arranged by size:
4742 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2253 4568 882
Size: 617 754 2315 2219
Fragments arranged by size:
2315 2219 754 617
BsaAI yAC'GTr
Cuts at: 493 1204 2143 3696 493
Size: 711 939 1553 2702
Fragments arranged by size:
2702 1553 939 711
BsaBI GATnn'nnATC
Cuts at: 2642 5899 2642
Size: 3257 2648
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2238 2249 3712 4094
Size: 53 83 186 936 705 11 1463 382
Cuts at: 4094 4729 275
Size: 635 1451
Fragments arranged by size:
1463 1451 936 705 635 382 186 83
53 11
BsaWI w'CCGG_w
Cuts at: 4338 4736 5010 5157 5568 4338
Size: 398 274 147 411 4675
Fragments arranged by size:
4675 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5680
Fragments arranged by size:
5680 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2438 2997 1186
Size: 1252 559 4094
Fragments arranged by size:
4094 1252 559
BseYI C'CCAG_C
Cuts at: 3122 5108 5604 3122
Size: 1986 496 3423
Fragments arranged by size:
3423 1986 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2341 2832 1163
Size: 573 605 491 4236
Fragments arranged by size:
4236 605 573 491
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2124 2314 2406 3355 4116 5144
Size: 275 186 123 190 92 949 761 1028
Cuts at: 5144 1540
Size: 2301
Fragments arranged by size:
2301 1028 949 761 275 190 186 123
92
BsiHKAI G_wGCw'C
Cuts at: 3966 4051 5122 3966
Size: 85 1071 4749
Fragments arranged by size:
4749 1071 85
BsmI GAATG_Cn'
Cuts at: 3416 3509 3416
Size: 93 5812
BsmBI CGTCTCn'nnnn_
Cuts at: 2182 2182
Size: 5905
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2041 2088 2095 2107 2301 2307 3224
Size: 399 251 47 7 12 194 6 917
Cuts at: 3224 3966 4051 5122 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 917 742 399 251 194 85
47 12 7 6
BspEI T'CCGG_A
Cuts at: 5568 5568
Size: 5905
BspHI T'CATG_A
Cuts at: 3794 5524 3794
Size: 1730 4175
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3260 844
Size: 2416 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3792 1394
Size: 429 1969 3507
Fragments arranged by size:
3507 1969 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2619 3155 4400 4574 65
Size: 1183 1371 536 1245 174 1396
Fragments arranged by size:
1396 1371 1245 1183 536 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3167 4549 4736 1700
Size: 110 1357 1382 187 2869
Fragments arranged by size:
2869 1382 1357 187 110
BsrGI T'GTAC_A
Cuts at: 96 2578 2980 96
Size: 2482 402 3021
Fragments arranged by size:
3021 2482 402
BssHII G'CGCG_C
Cuts at: 1925 2220 2274 1925
Size: 295 54 5556
Fragments arranged by size:
5556 295 54
BssSI C'ACGA_G
Cuts at: 2346 3965 4977 2346
Size: 1619 1012 3274
Fragments arranged by size:
3274 1619 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5905
BstBI TT'CG_AA
Cuts at: 4709 4709
Size: 5905
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5905
BstYI r'GATC_y
Cuts at: 1972 3019 3235 3602 3987 4004 5456 5542 5554
Size: 1047 216 367 385 17 1452 86 12
Cuts at: 5554 5900 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1047 385 367 346 216 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2124 2243 2897 513
Size: 552 939 120 119 654 3521
Fragments arranged by size:
3521 939 654 552 120 119
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5166
Fragments arranged by size:
5166 683 56
BtsI GCAGTG_nn'
Cuts at: 2820 3421 4215 4235 2820
Size: 601 794 20 4490
Fragments arranged by size:
4490 794 601 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5905
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5870
DraI TTT'AAA
Cuts at: 3130 3209 3130
Size: 79 5826
DraIII CAC_nnn'GTG
Cuts at: 3116 3116
Size: 5905
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4912 809
Size: 507 3596 1802
Fragments arranged by size:
3596 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2121 2188 2311
Size: 54 1425 50 275 18 291 67 123
Cuts at: 2311 2403 2616 2746 3352 4241 5870 8
Size: 92 213 130 606 889 1629 43
Fragments arranged by size:
1629 1425 889 606 291 275 213 130
123 92 67 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2121 2311 2403 3352 1537
Size: 275 309 190 92 949 4090
Fragments arranged by size:
4090 949 309 275 190 92
EarI CTCTTCn'nnn_
Cuts at: 1353 2503 3835 1353
Size: 1150 1332 3423
Fragments arranged by size:
3423 1332 1150
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2271 3207 4492 4866 5012 387
Size: 1064 138 682 936 1285 374 146 1280
Fragments arranged by size:
1285 1280 1064 936 682 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2982 3968 4565 5352 5620 1239
Size: 393 1350 986 597 787 268 1524
Fragments arranged by size:
1524 1350 986 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2091 2091
Size: 5905
EcoRI G'AATT_C
Cuts at: 2538 2853 3214 3592 2538
Size: 315 361 378 4851
Fragments arranged by size:
4851 378 361 315
FspI TGC'GCA
Cuts at: 4411 4411
Size: 5905
HaeII r_GCGC'y
Cuts at: 1678 2252 2431 5052 1678
Size: 574 179 2621 2531
Fragments arranged by size:
2621 2531 574 179
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2482 2514 2695 2727 2980
Size: 32 135 32 910 32 181 32 253
Cuts at: 2980 3012 4546 4578 4620 4652 1373
Size: 32 1534 32 42 32 2626
Fragments arranged by size:
2626 1534 910 253 181 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3249 3495 669
Size: 988 281 1311 246 3079
Fragments arranged by size:
3079 1311 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5905
HpaI GTT'AAC
Cuts at: 3495 3495
Size: 5905
KasI G'GCGC_C
Cuts at: 2248 2248
Size: 5905
KpnI G_GTAC'C
Cuts at: 3230 3230
Size: 5905
MfeI C'AATT_G
Cuts at: 3504 3504
Size: 5905
MluI A'CGCG_T
Cuts at: 5781 5781
Size: 5905
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5019 5203 1305
Size: 3714 184 2007
Fragments arranged by size:
3714 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2618 2748 10
Size: 54 1425 343 786 130 3167
Fragments arranged by size:
3167 1425 786 343 130 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2633 2636 3863 4222 4381 518
Size: 648 1467 3 1227 359 159 2042
Fragments arranged by size:
2042 1467 1227 648 359 159 3
MspA1I CmG'CkG
Cuts at: 1606 2595 3026 3321 3998 5146 5391 1606
Size: 989 431 295 677 1148 245 2120
Fragments arranged by size:
2120 1148 989 677 431 295 245
NaeI GCC'GGC
Cuts at: 3169 3169
Size: 5905
NarI GG'CG_CC
Cuts at: 2249 2249
Size: 5905
NcoI C'CATG_G
Cuts at: 513 1065 2004 2897 513
Size: 552 939 893 3521
Fragments arranged by size:
3521 939 893 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5905
NgoMIV G'CCGG_C
Cuts at: 3167 3167
Size: 5905
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5905
NotI GC'GGCC_GC
Cuts at: 3352 3352
Size: 5905
NsiI A_TGCA'T
Cuts at: 2891 2891
Size: 5905
NspI r_CATG'y
Cuts at: 1132 1229 2579 2922 3263 4808 1132
Size: 97 1350 343 341 1545 2229
Fragments arranged by size:
2229 1545 1350 343 341 97
PasI CC'CwG_GG
Cuts at: 1348 2328 1348
Size: 980 4925
PciI A'CATG_T
Cuts at: 2918 4804 2918
Size: 1886 4019
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 1108
Size: 902 5003
PfoI T'CCnGG_A
Cuts at: 3010 3010
Size: 5905
PmeI GTTT'AAAC
Cuts at: 3209 3209
Size: 5905
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3974 4006 5487 5519 3974
Size: 32 1481 32 4360
Fragments arranged by size:
4360 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5905
PsiI TTA'TAA
Cuts at: 3475 3475
Size: 5905
PspOMI G'GGCC_C
Cuts at: 2091 2091
Size: 5905
PspXI vC'TCGA_Gb
Cuts at: 2264 2264
Size: 5905
PstI C_TGCA'G
Cuts at: 830 3257 830
Size: 2427 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5905
PvuII CAG'CTG
Cuts at: 3026 3026
Size: 5905
SalI G'TCGA_C
Cuts at: 1936 3247 1936
Size: 1311 4594
SbfI CC_TGCA'GG
Cuts at: 3257 3257
Size: 5905
ScaI AGT'ACT
Cuts at: 4153 5865 4153
Size: 1712 4193
SfcI C'TryA_G
Cuts at: 826 1045 3253 4388 5069 5260 826
Size: 219 2208 1135 681 191 1471
Fragments arranged by size:
2208 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2250 2250
Size: 5905
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5905
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5905
SmaI CCC'GGG
Cuts at: 1872 2089 2096 3232 1872
Size: 217 7 1136 4545
Fragments arranged by size:
4545 1136 217 7
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2108 2264 4009 4910 5172 820
Size: 197 926 165 156 1745 901 262 1553
Fragments arranged by size:
1745 1553 926 901 262 197 165 156
SnaBI TAC'GTA
Cuts at: 493 3696 493
Size: 3203 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5905
SphI G_CATG'C
Cuts at: 1132 3263 1132
Size: 2131 3774
SspI AAT'ATT
Cuts at: 5 52 2664 3829 5
Size: 47 2612 1165 2081
Fragments arranged by size:
2612 2081 1165 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5905
StyI C'CwwG_G
Cuts at: 513 1065 2004 2211 2897 2953 3344 513
Size: 552 939 207 686 56 391 3074
Fragments arranged by size:
3074 939 686 552 391 207 56
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3932 3949 4102 4287 4732 325
Size: 1255 2352 17 153 185 445 1498
Fragments arranged by size:
2352 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2578 2980 4151
Size: 274 80 33 51 1443 601 402 1171
Cuts at: 4151 5863 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1171 601 402 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2463 2495 5865 5897 2463
Size: 32 3370 32 2471
Fragments arranged by size:
3370 2471 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5905
XbaI T'CTAG_A
Cuts at: 3241 3241
Size: 5905
XhoI C'TCGA_G
Cuts at: 1943 2108 2264 1943
Size: 165 156 5584
Fragments arranged by size:
5584 165 156
XmaI C'CCGG_G
Cuts at: 1870 2087 2094 3230 1870
Size: 217 7 1136 4545
Fragments arranged by size:
4545 1136 217 7
XmnI GAAnn'nnTTC
Cuts at: 4034 4034
Size: 5905
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3713 276
Size: 53 83 186 936 2179 2468
Fragments arranged by size:
2468 2179 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI BaeI BbvCI Bpu10I BsiWI BstXI
BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI
NruI PacI PmlI PpuMI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SrfI SwaI XcmI