Restriction map for
pFN21AB8706
(Circular) (Six-base) MAPSORT of: pFN21AB8706.seq Check: 6816 from: 1 to: 6175
pFN21AB8706 6175 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3985 278
Size: 53 83 186 936 2449 2468
Fragments arranged by size:
2468 2449 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3518 1937
Size: 1581 4594
Acc65I G'GTAC_C
Cuts at: 3496 3496
Size: 6175
AclI AA'CG_TT
Cuts at: 1563 4302 4675 1563
Size: 2739 373 3063
Fragments arranged by size:
3063 2739 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2493 2844 4238 5622 5890 1632
Size: 861 351 1394 1384 268 1917
Fragments arranged by size:
1917 1394 1384 861 351 268
AflII C'TTAA_G
Cuts at: 820 1017 2828 820
Size: 197 1811 4167
Fragments arranged by size:
4167 1811 197
AflIII A'CryG_T
Cuts at: 1203 5074 6051 1203
Size: 3871 977 1327
Fragments arranged by size:
3871 1327 977
AgeI A'CCGG_T
Cuts at: 5006 5006
Size: 6175
AhdI GACnn_n'nnGTC
Cuts at: 2023 4904 2023
Size: 2881 3294
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6175
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6175
AlwNI CAG_nnn'CTG
Cuts at: 3918 5490 3918
Size: 1572 4603
ApaLI G'TGCA_C
Cuts at: 4232 5388 4232
Size: 1156 5019
ApoI r'AATT_y
Cuts at: 1493 2712 2875 3283 3350 3484 3710 3862 5971
Size: 1219 163 408 67 134 226 152 2109
Cuts at: 5971 1493
Size: 1697
Fragments arranged by size:
2109 1697 1219 408 226 163 152 134
67
AseI AT'TA_AT
Cuts at: 160 1032 4729 160
Size: 872 3697 1606
Fragments arranged by size:
3697 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6175
AvaI C'yCGr_G
Cuts at: 1870 1943 3500 1870
Size: 73 1557 4545
Fragments arranged by size:
4545 1557 73
AvrII C'CTAG_G
Cuts at: 3388 3388
Size: 6175
BamHI G'GATC_C
Cuts at: 3505 3872 3505
Size: 367 5808
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3496 4951 618
Size: 325 217 627 1709 1455 1842
Fragments arranged by size:
1842 1709 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2440 3200 3494 1391
Size: 1049 760 294 4072
Fragments arranged by size:
4072 1049 760 294
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2703 2760 928
Size: 971 804 57 4343
Fragments arranged by size:
4343 971 804 57
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4366 4400 1903
Size: 34 2429 34 3678
Fragments arranged by size:
3678 2429 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2895 4067 5277 2895
Size: 1172 1210 3793
Fragments arranged by size:
3793 1210 1172
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6175
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4786 136
Size: 107 122 71 631 605 3114 1525
Fragments arranged by size:
3114 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6170 6170
Size: 6175
BlpI GC'TnA_GC
Cuts at: 3592 3592
Size: 6175
Bme1580I G_kGCm'C
Cuts at: 1790 4236 5392 1790
Size: 2446 1156 2573
Fragments arranged by size:
2573 2446 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6175
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3236 3906 4864
Size: 416 53 83 399 24 1819 670 958
Cuts at: 4864 442
Size: 1753
Fragments arranged by size:
1819 1753 958 670 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6175
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3219 3219
Size: 6175
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2729 4835 1239
Size: 1490 2106 2579
Fragments arranged by size:
2579 2106 1490
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4300 5165 5463 4300
Size: 865 298 5012
Fragments arranged by size:
5012 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4838 882
Size: 617 3339 2219
Fragments arranged by size:
3339 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3966 493
Size: 711 2762 2702
Fragments arranged by size:
2762 2702 711
BsaBI GATnn'nnATC
Cuts at: 6169 6169
Size: 6175
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3982 4364 4999 275
Size: 53 83 186 936 2449 382 635 1451
Fragments arranged by size:
2449 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4608 5006 5280 5427 5838 4608
Size: 398 274 147 411 4945
Fragments arranged by size:
4945 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5950
Fragments arranged by size:
5950 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6175
BseYI C'CCAG_C
Cuts at: 2219 3277 5378 5874 2219
Size: 1058 2101 496 2520
Fragments arranged by size:
2520 2101 1058 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5602
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3625 4386 5414 1540
Size: 275 186 1624 761 1028 2301
Fragments arranged by size:
2301 1624 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2198 3200 4236 4321 5392 2198
Size: 1002 1036 85 1071 2981
Fragments arranged by size:
2981 1071 1036 1002 85
BsmI GAATG_Cn'
Cuts at: 3686 3779 3686
Size: 93 6082
BsmBI CGTCTCn'nnnn_
Cuts at: 2743 2743
Size: 6175
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2198 2440 3200 3494 4236 4321 5392
Size: 399 408 242 760 294 742 85 1071
Cuts at: 5392 1391
Size: 2174
Fragments arranged by size:
2174 1071 760 742 408 399 294 242
85
BspEI T'CCGG_A
Cuts at: 5838 5838
Size: 6175
BspHI T'CATG_A
Cuts at: 4064 5794 4064
Size: 1730 4445
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3530 844
Size: 2686 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4062 1394
Size: 429 2239 3507
Fragments arranged by size:
3507 2239 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2263 2985 4670 4844 65
Size: 1183 1015 722 1685 174 1396
Fragments arranged by size:
1685 1396 1183 1015 722 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4819 5006 1700
Size: 110 3009 187 2869
Fragments arranged by size:
3009 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2627 96
Size: 2531 3644
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6175
BssSI C'ACGA_G
Cuts at: 4235 5247 4235
Size: 1012 5163
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6175
BstBI TT'CG_AA
Cuts at: 4979 4979
Size: 6175
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6175
BstYI r'GATC_y
Cuts at: 1972 3505 3872 4257 4274 5726 5812 5824 6170
Size: 1533 367 385 17 1452 86 12 346
Cuts at: 6170 1972
Size: 1977
Fragments arranged by size:
1977 1533 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4684
Fragments arranged by size:
4684 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5436
Fragments arranged by size:
5436 683 56
BtsI GCAGTG_nn'
Cuts at: 2217 3691 4485 4505 2217
Size: 1474 794 20 3887
Fragments arranged by size:
3887 1474 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6175
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6140
DraI TTT'AAA
Cuts at: 3479 3479
Size: 6175
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5182 809
Size: 507 3866 1802
Fragments arranged by size:
3866 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3622 4511 6140
Size: 54 1425 50 275 18 1792 889 1629
Cuts at: 6140 8
Size: 43
Fragments arranged by size:
1792 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3622 1537
Size: 275 1810 4090
Fragments arranged by size:
4090 1810 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4105 1353
Size: 2752 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4762 5136 5282 387
Size: 1064 138 3173 374 146 1280
Fragments arranged by size:
3173 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3198 3198
Size: 6175
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2493 2729 2844 4238 4835 5622 5890
Size: 393 861 236 115 1394 597 787 268
Cuts at: 5890 1239
Size: 1524
Fragments arranged by size:
1524 1394 861 787 597 393 268 236
115
EcoNI CCTnn'n_nnAGG
Cuts at: 2423 3006 2423
Size: 583 5592
EcoO109I rG'GnC_Cy
Cuts at: 2999 3385 2999
Size: 386 5789
EcoRI G'AATT_C
Cuts at: 3484 3862 3484
Size: 378 5797
FspI TGC'GCA
Cuts at: 4681 4681
Size: 6175
HaeII r_GCGC'y
Cuts at: 1678 5322 1678
Size: 3644 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2009 2041 3058 3090 4816
Size: 32 135 32 437 32 1017 32 1726
Cuts at: 4816 4848 4890 4922 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1726 1017 437 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2173 3519 3765 669
Size: 988 281 235 1346 246 3079
Fragments arranged by size:
3079 1346 988 281 246 235
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6175
HpaI GTT'AAC
Cuts at: 3765 3765
Size: 6175
KpnI G_GTAC'C
Cuts at: 3500 3500
Size: 6175
MfeI C'AATT_G
Cuts at: 3774 3774
Size: 6175
MluI A'CGCG_T
Cuts at: 6051 6051
Size: 6175
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2376 2778 5289 5473 1305
Size: 1071 402 2511 184 2007
Fragments arranged by size:
2511 2007 1071 402 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4353
Fragments arranged by size:
4353 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2660 3152 4133 4492 4651 518
Size: 648 1494 492 981 359 159 2042
Fragments arranged by size:
2042 1494 981 648 492 359 159
MspA1I CmG'CkG
Cuts at: 1606 2219 2891 3591 4268 5416 5661 1606
Size: 613 672 700 677 1148 245 2120
Fragments arranged by size:
2120 1148 700 677 672 613 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4684
Fragments arranged by size:
4684 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6175
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6175
NotI GC'GGCC_GC
Cuts at: 3622 3622
Size: 6175
NspI r_CATG'y
Cuts at: 1132 1229 3533 5078 1132
Size: 97 2304 1545 2229
Fragments arranged by size:
2304 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6175
PciI A'CATG_T
Cuts at: 5074 5074
Size: 6175
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6175
PmeI GTTT'AAAC
Cuts at: 3479 3479
Size: 6175
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4244 4276 5757 5789 4244
Size: 32 1481 32 4630
Fragments arranged by size:
4630 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2456 1613
Size: 843 5332
PsiI TTA'TAA
Cuts at: 3745 3745
Size: 6175
PstI C_TGCA'G
Cuts at: 830 3364 3527 830
Size: 2534 163 3478
Fragments arranged by size:
3478 2534 163
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6175
PvuII CAG'CTG
Cuts at: 2219 2219
Size: 6175
SacI G_AGCT'C
Cuts at: 3200 3200
Size: 6175
SalI G'TCGA_C
Cuts at: 1936 3517 1936
Size: 1581 4594
SbfI CC_TGCA'GG
Cuts at: 3527 3527
Size: 6175
ScaI AGT'ACT
Cuts at: 4423 6135 4423
Size: 1712 4463
SfcI C'TryA_G
Cuts at: 826 1045 3103 3360 3523 4658 5339 5530 826
Size: 219 2058 257 163 1135 681 191 1471
Fragments arranged by size:
2058 1471 1135 681 257 219 191 163
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6175
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6175
SmaI CCC'GGG
Cuts at: 1872 3502 1872
Size: 1630 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2828 4279 5180 5442 820
Size: 197 926 885 1451 901 262 1553
Fragments arranged by size:
1553 1451 926 901 885 262 197
SnaBI TAC'GTA
Cuts at: 493 3966 493
Size: 3473 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6175
SphI G_CATG'C
Cuts at: 1132 3533 1132
Size: 2401 3774
SspI AAT'ATT
Cuts at: 5 52 2950 4099 5
Size: 47 2898 1149 2081
Fragments arranged by size:
2898 2081 1149 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6175
StyI C'CwwG_G
Cuts at: 513 1065 2004 3388 3614 513
Size: 552 939 1384 226 3074
Fragments arranged by size:
3074 1384 939 552 226
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4202 4219 4372 4557 5002 325
Size: 1255 2622 17 153 185 445 1498
Fragments arranged by size:
2622 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2627 4421 6133
Size: 274 80 33 51 1443 650 1794 1712
Cuts at: 6133 96
Size: 138
Fragments arranged by size:
1794 1712 1443 650 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3162 3194 6135 6167 3162
Size: 32 2941 32 3170
Fragments arranged by size:
3170 2941 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6175
XbaI T'CTAG_A
Cuts at: 3511 3511
Size: 6175
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6175
XmaI C'CCGG_G
Cuts at: 1870 3500 1870
Size: 1630 4545
XmnI GAAnn'nnTTC
Cuts at: 4304 4304
Size: 6175
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3983 276
Size: 53 83 186 936 2449 2468
Fragments arranged by size:
2468 2449 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI