Restriction map for
pFN21AB8705
(Circular) (Six-base) MAPSORT of: pFN21AB8705.seq Check: 4527 from: 1 to: 5833
pFN21AB8705 5833 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3643 278
Size: 53 83 186 936 2107 2468
Fragments arranged by size:
2468 2107 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3176 1937
Size: 1239 4594
Acc65I G'GTAC_C
Cuts at: 3154 3154
Size: 5833
AclI AA'CG_TT
Cuts at: 1563 3960 4333 1563
Size: 2397 373 3063
Fragments arranged by size:
3063 2397 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3896 5280 5548 1632
Size: 2264 1384 268 1917
Fragments arranged by size:
2264 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5636
AflIII A'CryG_T
Cuts at: 1203 2214 4732 5709 1203
Size: 1011 2518 977 1327
Fragments arranged by size:
2518 1327 1011 977
AgeI A'CCGG_T
Cuts at: 4664 4664
Size: 5833
AhdI GACnn_n'nnGTC
Cuts at: 4562 4562
Size: 5833
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5833
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5833
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2977 3009 2977
Size: 32 5801
AlwNI CAG_nnn'CTG
Cuts at: 2258 3576 5148 2258
Size: 1318 1572 2943
Fragments arranged by size:
2943 1572 1318
ApaLI G'TGCA_C
Cuts at: 3890 5046 3890
Size: 1156 4677
ApoI r'AATT_y
Cuts at: 1493 2782 3142 3368 3520 5629 1493
Size: 1289 360 226 152 2109 1697
Fragments arranged by size:
2109 1697 1289 360 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2882 3023 4387 160
Size: 872 1850 141 1364 1606
Fragments arranged by size:
1850 1606 1364 872 141
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5833
AvaI C'yCGr_G
Cuts at: 1870 1943 2071 3158 1870
Size: 73 128 1087 4545
Fragments arranged by size:
4545 1087 128 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2930 2963 2930
Size: 33 5800
BamHI G'GATC_C
Cuts at: 3163 3530 3163
Size: 367 5466
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3154 4609 618
Size: 325 217 627 1367 1455 1842
Fragments arranged by size:
1842 1455 1367 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3152 1391
Size: 1761 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4862
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2213 2247 4024 4058 1903
Size: 34 276 34 1777 34 3678
Fragments arranged by size:
3678 1777 276 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3725 4935 3725
Size: 1210 4623
BclI T'GATC_A
Cuts at: 1548 2623 1548
Size: 1075 4758
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2240 4444 136
Size: 107 122 71 631 605 568 2204 1525
Fragments arranged by size:
2204 1525 631 605 568 122 107 71
BglII A'GATC_T
Cuts at: 2067 5828 2067
Size: 3761 2072
BlpI GC'TnA_GC
Cuts at: 3250 3250
Size: 5833
Bme1580I G_kGCm'C
Cuts at: 1790 3894 5050 1790
Size: 2104 1156 2573
Fragments arranged by size:
2573 2104 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5833
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3564 4522 442
Size: 416 53 83 399 24 2147 958 1753
Fragments arranged by size:
2147 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5833
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2913 2913
Size: 5833
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2197 2388 3111 4493 1239
Size: 958 191 723 1382 2579
Fragments arranged by size:
2579 1382 958 723 191
Bpu10I CC'TnA_GC
Cuts at: 2188 2188
Size: 5833
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3958 4823 5121 3958
Size: 865 298 4670
Fragments arranged by size:
4670 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4496 882
Size: 617 2997 2219
Fragments arranged by size:
2997 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3109 3624 493
Size: 711 1905 515 2702
Fragments arranged by size:
2702 1905 711 515
BsaBI GATnn'nnATC
Cuts at: 5827 5827
Size: 5833
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3640 4022 4657 275
Size: 53 83 186 936 2107 382 635 1451
Fragments arranged by size:
2107 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4266 4664 4938 5085 5496 4266
Size: 398 274 147 411 4603
Fragments arranged by size:
4603 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5608
Fragments arranged by size:
5608 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2886 1186
Size: 1700 4133
BseYI C'CCAG_C
Cuts at: 2690 5036 5532 2690
Size: 2346 496 2991
Fragments arranged by size:
2991 2346 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2595 1163
Size: 573 859 4401
Fragments arranged by size:
4401 859 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3283 4044 5072 1540
Size: 275 186 1282 761 1028 2301
Fragments arranged by size:
2301 1282 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2551 3894 3979 5050 2551
Size: 1343 85 1071 3334
Fragments arranged by size:
3334 1343 1071 85
BsmI GAATG_Cn'
Cuts at: 3344 3437 3344
Size: 93 5740
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2551 3152 3894 3979 5050 1391
Size: 399 761 601 742 85 1071 2174
Fragments arranged by size:
2174 1071 761 742 601 399 85
BspEI T'CCGG_A
Cuts at: 5496 5496
Size: 5833
BspHI T'CATG_A
Cuts at: 2656 2772 3722 5452 2656
Size: 116 950 1730 3037
Fragments arranged by size:
3037 1730 950 116
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3188 844
Size: 2344 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2312 3720 1394
Size: 429 489 1408 3507
Fragments arranged by size:
3507 1408 489 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2578 4328 4502 65
Size: 1183 1330 1750 174 1396
Fragments arranged by size:
1750 1396 1330 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4477 4664 1700
Size: 110 2667 187 2869
Fragments arranged by size:
2869 2667 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5833
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5833
BssSI C'ACGA_G
Cuts at: 3893 4905 3893
Size: 1012 4821
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3038 794
Size: 2244 3589
BstBI TT'CG_AA
Cuts at: 4637 4637
Size: 5833
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5833
BstYI r'GATC_y
Cuts at: 1972 2067 2803 3163 3530 3915 3932 5384 5470
Size: 95 736 360 367 385 17 1452 86
Cuts at: 5470 5482 5828 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 736 385 367 360 346 95
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2518 3129 513
Size: 552 1453 611 3217
Fragments arranged by size:
3217 1453 611 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5094
Fragments arranged by size:
5094 683 56
BtsI GCAGTG_nn'
Cuts at: 2229 2355 3349 4143 4163 2229
Size: 126 994 794 20 3899
Fragments arranged by size:
3899 994 794 126 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5833
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5798
DraI TTT'AAA
Cuts at: 3137 3137
Size: 5833
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4840 809
Size: 507 3524 1802
Fragments arranged by size:
3524 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2515 2566 3280
Size: 54 1425 50 275 18 685 51 714
Cuts at: 3280 4169 5798 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 714 685 275 54 51
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3280 1537
Size: 275 1468 4090
Fragments arranged by size:
4090 1468 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3763 1353
Size: 2410 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4420 4794 4940 387
Size: 1064 138 2831 374 146 1280
Fragments arranged by size:
2831 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2197 2388 3111 3896 4493 5280 5548
Size: 393 565 191 723 785 597 787 268
Cuts at: 5548 1239
Size: 1524
Fragments arranged by size:
1524 787 785 723 597 565 393 268
191
EcoO109I rG'GnC_Cy
Cuts at: 2687 3012 2687
Size: 325 5508
EcoRI G'AATT_C
Cuts at: 3142 3520 3142
Size: 378 5455
FspI TGC'GCA
Cuts at: 4339 4339
Size: 5833
HaeII r_GCGC'y
Cuts at: 1678 4980 1678
Size: 3302 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2276 2308 2651 2683 2788
Size: 32 135 32 704 32 343 32 105
Cuts at: 2788 2820 4474 4506 4548 4580 1373
Size: 32 1654 32 42 32 2626
Fragments arranged by size:
2626 1654 704 343 135 105 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3177 3423 669
Size: 988 281 1239 246 3079
Fragments arranged by size:
3079 1239 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5833
HpaI GTT'AAC
Cuts at: 3423 3423
Size: 5833
KpnI G_GTAC'C
Cuts at: 3158 3158
Size: 5833
MfeI C'AATT_G
Cuts at: 3432 3432
Size: 5833
MluI A'CGCG_T
Cuts at: 5709 5709
Size: 5833
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2121 2226 2854 4947 5131 1305
Size: 816 105 628 2093 184 2007
Fragments arranged by size:
2093 2007 816 628 184 105
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2517 2568 10
Size: 54 1425 343 685 51 3275
Fragments arranged by size:
3275 1425 685 343 54 51
MslI CAynn'nnrTG
Cuts at: 518 1166 2505 2568 2589 2619 2625 2631 2637
Size: 648 1339 63 21 30 6 6 6
Cuts at: 2637 2655 2774 3791 4150 4309 518
Size: 18 119 1017 359 159 2042
Fragments arranged by size:
2042 1339 1017 648 359 159 119 63
30 21 18 6 6 6
MspA1I CmG'CkG
Cuts at: 1606 3249 3926 5074 5319 1606
Size: 1643 677 1148 245 2120
Fragments arranged by size:
2120 1643 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 3129 513
Size: 552 2064 3217
Fragments arranged by size:
3217 2064 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5833
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5833
NotI GC'GGCC_GC
Cuts at: 3280 3280
Size: 5833
NspI r_CATG'y
Cuts at: 1132 1229 2218 3191 4736 1132
Size: 97 989 973 1545 2229
Fragments arranged by size:
2229 1545 989 973 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5833
PciI A'CATG_T
Cuts at: 2214 4732 2214
Size: 2518 3315
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5833
PfoI T'CCnGG_A
Cuts at: 2108 2108
Size: 5833
PmeI GTTT'AAAC
Cuts at: 3137 3137
Size: 5833
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2301 2333 3902 3934 5415 5447 2301
Size: 32 1569 32 1481 32 2687
Fragments arranged by size:
2687 1569 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2687 3012 2687
Size: 325 5508
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5833
PsiI TTA'TAA
Cuts at: 3403 3403
Size: 5833
PstI C_TGCA'G
Cuts at: 830 3185 830
Size: 2355 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5833
SalI G'TCGA_C
Cuts at: 1936 3175 1936
Size: 1239 4594
SbfI CC_TGCA'GG
Cuts at: 3185 3185
Size: 5833
ScaI AGT'ACT
Cuts at: 4081 5793 4081
Size: 1712 4121
SfcI C'TryA_G
Cuts at: 826 1045 3181 4316 4997 5188 826
Size: 219 2136 1135 681 191 1471
Fragments arranged by size:
2136 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5833
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5833
SmaI CCC'GGG
Cuts at: 1872 3160 1872
Size: 1288 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3937 4838 5100 820
Size: 197 926 1994 901 262 1553
Fragments arranged by size:
1994 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3109 3624 493
Size: 2616 515 2702
Fragments arranged by size:
2702 2616 515
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5833
SphI G_CATG'C
Cuts at: 1132 3191 1132
Size: 2059 3774
SspI AAT'ATT
Cuts at: 5 52 2472 2880 3076 3757 5
Size: 47 2420 408 196 681 2081
Fragments arranged by size:
2420 2081 681 408 196 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5833
StyI C'CwwG_G
Cuts at: 513 1065 3129 3272 513
Size: 552 2064 143 3074
Fragments arranged by size:
3074 2064 552 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2059 3860 3877 4030 4215 4660 325
Size: 1255 479 1801 17 153 185 445 1498
Fragments arranged by size:
1801 1498 1255 479 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4079 5791 96
Size: 274 80 33 51 1443 2102 1712 138
Fragments arranged by size:
2102 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2516 2548 5793 5825 2516
Size: 32 3245 32 2524
Fragments arranged by size:
3245 2524 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2526 1316
Size: 1210 4623
XbaI T'CTAG_A
Cuts at: 2551 3169 2551
Size: 618 5215
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5833
XmaI C'CCGG_G
Cuts at: 1870 3158 1870
Size: 1288 4545
XmnI GAAnn'nnTTC
Cuts at: 3962 3962
Size: 5833
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3641 276
Size: 53 83 186 936 2107 2468
Fragments arranged by size:
2468 2107 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BbeI BbvCI BfrBI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI