Restriction map for
pFN21AB8698
(Circular) (Six-base) MAPSORT of: pFN21AB8698.seq Check: 4787 from: 1 to: 6460
pFN21AB8698 6460 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4270 278
Size: 53 83 186 936 2734 2468
Fragments arranged by size:
2734 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3803 1937
Size: 1866 4594
Acc65I G'GTAC_C
Cuts at: 3616 3781 3616
Size: 165 6295
AclI AA'CG_TT
Cuts at: 1563 4587 4960 1563
Size: 3024 373 3063
Fragments arranged by size:
3063 3024 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3263 3382 4523 5907 6175 1632
Size: 1631 119 1141 1384 268 1917
Fragments arranged by size:
1917 1631 1384 1141 268 119
AflII C'TTAA_G
Cuts at: 820 1017 2119 820
Size: 197 1102 5161
Fragments arranged by size:
5161 1102 197
AflIII A'CryG_T
Cuts at: 1203 2417 3253 5359 6336 1203
Size: 1214 836 2106 977 1327
Fragments arranged by size:
2106 1327 1214 977 836
AgeI A'CCGG_T
Cuts at: 3586 5291 3586
Size: 1705 4755
AhdI GACnn_n'nnGTC
Cuts at: 5189 5189
Size: 6460
AleI CACnn'nnGTG
Cuts at: 1166 2018 1166
Size: 852 5608
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6460
AlwNI CAG_nnn'CTG
Cuts at: 3052 4203 5775 3052
Size: 1151 1572 3737
Fragments arranged by size:
3737 1572 1151
ApaLI G'TGCA_C
Cuts at: 4517 5673 4517
Size: 1156 5304
ApoI r'AATT_y
Cuts at: 1493 3503 3769 3995 4147 6256 1493
Size: 2010 266 226 152 2109 1697
Fragments arranged by size:
2109 2010 1697 266 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2924 5014 160
Size: 872 1892 2090 1606
Fragments arranged by size:
2090 1892 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6460
AvaI C'yCGr_G
Cuts at: 1870 1943 3785 1870
Size: 73 1842 4545
Fragments arranged by size:
4545 1842 73
BamHI G'GATC_C
Cuts at: 3790 4157 3790
Size: 367 6093
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2545 3616 3781 5236 618
Size: 325 217 627 758 1071 165 1455 1842
Fragments arranged by size:
1842 1455 1071 758 627 325 217 165
BanII G_rGCy'C
Cuts at: 1391 3779 1391
Size: 2388 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5489
BbvCI CC'TCA_GC
Cuts at: 2916 3006 2916
Size: 90 6370
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4651 4685 1903
Size: 34 2714 34 3678
Fragments arranged by size:
3678 2714 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4352 5562 4352
Size: 1210 5250
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6460
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5071 136
Size: 107 122 71 631 605 3399 1525
Fragments arranged by size:
3399 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6455 6455
Size: 6460
BlpI GC'TnA_GC
Cuts at: 3633 3877 3633
Size: 244 6216
Bme1580I G_kGCm'C
Cuts at: 1790 2682 4521 5677 1790
Size: 892 1839 1156 2573
Fragments arranged by size:
2573 1839 1156 892
BmgBI CAC'GTC
Cuts at: 1318 3254 1318
Size: 1936 4524
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2678 4191 5149
Size: 416 53 83 399 24 1261 1513 958
Cuts at: 5149 442
Size: 1753
Fragments arranged by size:
1753 1513 1261 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6460
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2262 5120 1239
Size: 1023 2858 2579
Fragments arranged by size:
2858 2579 1023
Bpu10I CC'TnA_GC
Cuts at: 2916 3006 2916
Size: 90 6370
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3594 4585 5450 5748 3594
Size: 991 865 298 4306
Fragments arranged by size:
4306 991 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2023 2238 3486 5123 882
Size: 617 524 215 1248 1637 2219
Fragments arranged by size:
2219 1637 1248 617 524 215
BsaAI yAC'GTr
Cuts at: 493 1204 2420 4251 493
Size: 711 1216 1831 2702
Fragments arranged by size:
2702 1831 1216 711
BsaBI GATnn'nnATC
Cuts at: 3237 6454 3237
Size: 3217 3243
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4267 4649 5284 275
Size: 53 83 186 936 2734 382 635 1451
Fragments arranged by size:
2734 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3586 4893 5291 5565 5712 6123 3586
Size: 1307 398 274 147 411 3923
Fragments arranged by size:
3923 1307 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6235
Fragments arranged by size:
6235 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2883 3063 3734 1186
Size: 1697 180 671 3912
Fragments arranged by size:
3912 1697 671 180
BseYI C'CCAG_C
Cuts at: 3018 3063 5663 6159 3018
Size: 45 2600 496 3319
Fragments arranged by size:
3319 2600 496 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2715 3053 1163
Size: 573 979 338 4570
Fragments arranged by size:
4570 979 573 338
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3910 4671 5699 1540
Size: 275 186 1909 761 1028 2301
Fragments arranged by size:
2301 1909 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4521 4606 5677 4521
Size: 85 1071 5304
Fragments arranged by size:
5304 1071 85
BsmI GAATG_Cn'
Cuts at: 3971 4064 3971
Size: 93 6367
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2682 3779 4521 4606 5677 1391
Size: 399 892 1097 742 85 1071 2174
Fragments arranged by size:
2174 1097 1071 892 742 399 85
BspEI T'CCGG_A
Cuts at: 6123 6123
Size: 6460
BspHI T'CATG_A
Cuts at: 4349 6079 4349
Size: 1730 4730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3815 844
Size: 2971 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3113 4347 1394
Size: 429 1290 1234 3507
Fragments arranged by size:
3507 1290 1234 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2533 2661 2724 2779 4955 5129 65
Size: 1183 1285 128 63 55 2176 174 1396
Fragments arranged by size:
2176 1396 1285 1183 174 128 63 55
BsrFI r'CCGG_y
Cuts at: 1700 1810 3586 5104 5291 1700
Size: 110 1776 1518 187 2869
Fragments arranged by size:
2869 1776 1518 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6460
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6460
BssSI C'ACGA_G
Cuts at: 4520 5532 4520
Size: 1012 5448
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3052 3184 794
Size: 2258 132 4070
Fragments arranged by size:
4070 2258 132
BstBI TT'CG_AA
Cuts at: 5264 5264
Size: 6460
BstEII G'GTnAC_C
Cuts at: 1184 2597 3589 1184
Size: 1413 992 4055
Fragments arranged by size:
4055 1413 992
BstXI CCAn_nnnn'nTGG
Cuts at: 2020 2715 3107 2020
Size: 695 392 5373
Fragments arranged by size:
5373 695 392
BstYI r'GATC_y
Cuts at: 1972 2164 2423 3519 3790 4157 4542 4559 6011
Size: 192 259 1096 271 367 385 17 1452
Cuts at: 6011 6097 6109 6455 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1096 385 367 346 271 259
192 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5908
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5721
Fragments arranged by size:
5721 683 56
BtsI GCAGTG_nn'
Cuts at: 2619 2653 2698 3124 3418 3976 4770 4790 2619
Size: 34 45 426 294 558 794 20 4289
Fragments arranged by size:
4289 794 558 426 294 45 34 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6460
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2922 2957 529
Size: 35 2358 35 4032
Fragments arranged by size:
4032 2358 35 35
DraI TTT'AAA
Cuts at: 3764 3764
Size: 6460
DraIII CAC_nnn'GTG
Cuts at: 3031 3031
Size: 6460
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5467 809
Size: 507 4151 1802
Fragments arranged by size:
4151 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3907 4796 6425
Size: 54 1425 50 275 18 2077 889 1629
Cuts at: 6425 8
Size: 43
Fragments arranged by size:
2077 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3907 1537
Size: 275 2095 4090
Fragments arranged by size:
4090 2095 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3401 3711 4390 1353
Size: 2048 310 679 3423
Fragments arranged by size:
3423 2048 679 310
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5047 5421 5567 387
Size: 1064 138 3458 374 146 1280
Fragments arranged by size:
3458 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2262 3263 3382 4523 5120 5907 6175
Size: 393 630 1001 119 1141 597 787 268
Cuts at: 6175 1239
Size: 1524
Fragments arranged by size:
1524 1141 1001 787 630 597 393 268
119
EcoO109I rG'GnC_Cy
Cuts at: 3002 3002
Size: 6460
EcoRI G'AATT_C
Cuts at: 3769 4147 3769
Size: 378 6082
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2469 2469
Size: 6460
FspI TGC'GCA
Cuts at: 4966 4966
Size: 6460
HaeII r_GCGC'y
Cuts at: 1678 5607 1678
Size: 3929 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5101 5133 5175 5207 1373
Size: 32 135 32 3529 32 42 32 2626
Fragments arranged by size:
3529 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2327 2366 3804 4050 669
Size: 988 281 389 39 1438 246 3079
Fragments arranged by size:
3079 1438 988 389 281 246 39
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6460
HpaI GTT'AAC
Cuts at: 4050 4050
Size: 6460
KpnI G_GTAC'C
Cuts at: 3620 3785 3620
Size: 165 6295
MfeI C'AATT_G
Cuts at: 4059 4059
Size: 6460
MluI A'CGCG_T
Cuts at: 6336 6336
Size: 6460
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5574 5758 1305
Size: 4269 184 2007
Fragments arranged by size:
4269 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4638
Fragments arranged by size:
4638 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2018 3423 4418 4777 4936 518
Size: 648 852 1405 995 359 159 2042
Fragments arranged by size:
2042 1405 995 852 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2240 2609 3052 3876 4553 5701 5946 1606
Size: 634 369 443 824 677 1148 245 2120
Fragments arranged by size:
2120 1148 824 677 634 443 369 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5908
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6460
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6460
NotI GC'GGCC_GC
Cuts at: 3907 3907
Size: 6460
NspI r_CATG'y
Cuts at: 1132 1229 2176 3818 5363 1132
Size: 97 947 1642 1545 2229
Fragments arranged by size:
2229 1642 1545 947 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6460
PciI A'CATG_T
Cuts at: 5359 5359
Size: 6460
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2198 2360 1108
Size: 1090 162 5208
Fragments arranged by size:
5208 1090 162
PmeI GTTT'AAAC
Cuts at: 3764 3764
Size: 6460
PmlI CAC'GTG
Cuts at: 2420 2420
Size: 6460
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4529 4561 6042 6074 4529
Size: 32 1481 32 4915
Fragments arranged by size:
4915 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3002 3002
Size: 6460
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6460
PsiI TTA'TAA
Cuts at: 3263 4030 3263
Size: 767 5693
PstI C_TGCA'G
Cuts at: 830 2614 3057 3812 830
Size: 1784 443 755 3478
Fragments arranged by size:
3478 1784 755 443
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6460
PvuII CAG'CTG
Cuts at: 2240 2609 3052 2240
Size: 369 443 5648
Fragments arranged by size:
5648 443 369
SalI G'TCGA_C
Cuts at: 1936 3802 1936
Size: 1866 4594
SbfI CC_TGCA'GG
Cuts at: 3812 3812
Size: 6460
ScaI AGT'ACT
Cuts at: 3325 4708 6420 3325
Size: 1383 1712 3365
Fragments arranged by size:
3365 1712 1383
SfcI C'TryA_G
Cuts at: 826 1045 2610 2625 3053 3808 4943 5624 5815
Size: 219 1565 15 428 755 1135 681 191
Cuts at: 5815 826
Size: 1471
Fragments arranged by size:
1565 1471 1135 755 681 428 219 191
15
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6460
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6460
SmaI CCC'GGG
Cuts at: 1872 3787 1872
Size: 1915 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2119 3609 4564 5465 5727 820
Size: 197 926 176 1490 955 901 262 1553
Fragments arranged by size:
1553 1490 955 926 901 262 197 176
SnaBI TAC'GTA
Cuts at: 493 4251 493
Size: 3758 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6460
SphI G_CATG'C
Cuts at: 1132 3818 1132
Size: 2686 3774
SspI AAT'ATT
Cuts at: 5 52 2830 4384 5
Size: 47 2778 1554 2081
Fragments arranged by size:
2778 2081 1554 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6460
StyI C'CwwG_G
Cuts at: 513 1065 2215 2766 3899 513
Size: 552 1150 551 1133 3074
Fragments arranged by size:
3074 1150 1133 552 551
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4487 4504 4657 4842 5287 325
Size: 1255 2907 17 153 185 445 1498
Fragments arranged by size:
2907 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2059 3323 4706
Size: 274 80 33 51 1443 82 1264 1383
Cuts at: 4706 6418 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1383 1264 274 138 82 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2213 2245 6420 6452 2213
Size: 32 4175 32 2221
Fragments arranged by size:
4175 2221 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6460
XbaI T'CTAG_A
Cuts at: 3796 3796
Size: 6460
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2773 2773
Size: 6460
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6460
XmaI C'CCGG_G
Cuts at: 1870 3785 1870
Size: 1915 4545
XmnI GAAnn'nnTTC
Cuts at: 4589 4589
Size: 6460
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4268 276
Size: 53 83 186 936 2734 2468
Fragments arranged by size:
2734 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI