Restriction map for
pFN21AB8697
(Circular) (Six-base) MAPSORT of: pFN21AB8697.seq Check: 1053 from: 1 to: 6343
pFN21AB8697 6343 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2691 2691
Size: 6343
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4153 278
Size: 53 83 186 936 2617 2468
Fragments arranged by size:
2617 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3686 1937
Size: 1749 4594
Acc65I G'GTAC_C
Cuts at: 3664 3664
Size: 6343
AclI AA'CG_TT
Cuts at: 1563 4470 4843 1563
Size: 2907 373 3063
Fragments arranged by size:
3063 2907 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3173 4406 5790 6058 1632
Size: 1541 1233 1384 268 1917
Fragments arranged by size:
1917 1541 1384 1233 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6146
AflIII A'CryG_T
Cuts at: 1203 5242 6219 1203
Size: 4039 977 1327
Fragments arranged by size:
4039 1327 977
AgeI A'CCGG_T
Cuts at: 3300 5174 3300
Size: 1874 4469
AhdI GACnn_n'nnGTC
Cuts at: 5072 5072
Size: 6343
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6343
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3180 1184
Size: 1996 4347
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3191 3223 3191
Size: 32 6311
AlwNI CAG_nnn'CTG
Cuts at: 3010 3466 4086 5658 3010
Size: 456 620 1572 3695
Fragments arranged by size:
3695 1572 620 456
ApaI G_GGCC'C
Cuts at: 2206 2288 2794 2206
Size: 82 506 5755
Fragments arranged by size:
5755 506 82
ApaLI G'TGCA_C
Cuts at: 4400 5556 4400
Size: 1156 5187
ApoI r'AATT_y
Cuts at: 1493 2617 3652 3878 4030 6139 1493
Size: 1124 1035 226 152 2109 1697
Fragments arranged by size:
2109 1697 1124 1035 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4897 160
Size: 872 3865 1606
Fragments arranged by size:
3865 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6343
AvaI C'yCGr_G
Cuts at: 1870 1943 2084 2793 3668 1870
Size: 73 141 709 875 4545
Fragments arranged by size:
4545 875 709 141 73
BamHI G'GATC_C
Cuts at: 2419 3673 4040 2419
Size: 1254 367 4722
Fragments arranged by size:
4722 1254 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3066 3664 5119 618
Size: 325 217 627 1279 598 1455 1842
Fragments arranged by size:
1842 1455 1279 627 598 325 217
BanII G_rGCy'C
Cuts at: 1391 2095 2179 2206 2288 2794 3488 3535 3627
Size: 704 84 27 82 506 694 47 92
Cuts at: 3627 3662 1391
Size: 35 4072
Fragments arranged by size:
4072 704 694 506 92 84 82 47
35 27
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2250 2974 928
Size: 971 351 724 4297
Fragments arranged by size:
4297 971 724 351
BbvCI CC'TCA_GC
Cuts at: 3345 3345
Size: 6343
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4534 4568 1903
Size: 34 2597 34 3678
Fragments arranged by size:
3678 2597 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4235 5445 4235
Size: 1210 5133
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6343
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2189 2711 3523
Size: 107 122 71 631 605 517 522 812
Cuts at: 3523 4954 136
Size: 1431 1525
Fragments arranged by size:
1525 1431 812 631 605 522 517 122
107 71
BglII A'GATC_T
Cuts at: 6338 6338
Size: 6343
BlpI GC'TnA_GC
Cuts at: 2228 3760 2228
Size: 1532 4811
Bme1580I G_kGCm'C
Cuts at: 1790 2206 2288 2305 2794 3424 4404 5560 1790
Size: 416 82 17 489 630 980 1156 2573
Fragments arranged by size:
2573 1156 980 630 489 416 82 17
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6343
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3621 4074 5032
Size: 416 53 83 399 24 2204 453 958
Cuts at: 5032 442
Size: 1753
Fragments arranged by size:
2204 1753 958 453 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6343
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2842 2905 3646 2842
Size: 63 741 5539
Fragments arranged by size:
5539 741 63
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2367 2624 3011 3299 3395 3425 5003 1239
Size: 1128 257 387 288 96 30 1578 2579
Fragments arranged by size:
2579 1578 1128 387 288 257 96 30
Bpu10I CC'TnA_GC
Cuts at: 3345 3345
Size: 6343
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2163 2747 2753 2915 3356 3519 3603 4468 5333
Size: 584 6 162 441 163 84 865 865
Cuts at: 5333 5631 2163
Size: 298 2875
Fragments arranged by size:
2875 865 865 584 441 298 163 162
84 6
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2454 2460 3137 5006 882
Size: 617 955 6 677 1869 2219
Fragments arranged by size:
2219 1869 955 677 617 6
BsaAI yAC'GTr
Cuts at: 493 1204 4134 493
Size: 711 2930 2702
Fragments arranged by size:
2930 2702 711
BsaBI GATnn'nnATC
Cuts at: 6337 6337
Size: 6343
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4150 4532 5167 275
Size: 53 83 186 936 2617 382 635 1451
Fragments arranged by size:
2617 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2071 2613 3300 4776 5174 5448 5595 6006 2071
Size: 542 687 1476 398 274 147 411 2408
Fragments arranged by size:
2408 1476 687 542 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2507 2537 2747 2777 3398
Size: 30 165 30 1104 30 210 30 621
Cuts at: 3398 3428 1178
Size: 30 4093
Fragments arranged by size:
4093 1104 621 210 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2148 2237 2576 2621 2813 2816 2819 2978
Size: 962 89 339 45 192 3 3 159
Cuts at: 2978 3203 3362 3526 1186
Size: 225 159 164 4003
Fragments arranged by size:
4003 962 339 225 192 164 159 159
89 45 3 3
BseYI C'CCAG_C
Cuts at: 2105 3542 5546 6042 2105
Size: 1437 2004 496 2406
Fragments arranged by size:
2406 2004 1437 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2804 1163
Size: 573 1068 4702
Fragments arranged by size:
4702 1068 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3793 4554 5582 1540
Size: 275 186 1792 761 1028 2301
Fragments arranged by size:
2301 1792 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2258 2613 2697 3586 3593 4404 4489 5560 2258
Size: 355 84 889 7 811 85 1071 3041
Fragments arranged by size:
3041 1071 889 811 355 85 84 7
BsmI GAATG_Cn'
Cuts at: 3854 3947 3854
Size: 93 6250
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2095 2179 2206 2258 2288 2305 2613
Size: 399 305 84 27 52 30 17 308
Cuts at: 2613 2697 2794 3424 3488 3535 3586 3593 3627
Size: 84 97 630 64 47 51 7 34
Cuts at: 3627 3662 4404 4489 5560 1391
Size: 35 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 630 399 308 305 97
85 84 84 64 52 51 47 35
34 30 27 17 7
BspEI T'CCGG_A
Cuts at: 6006 6006
Size: 6343
BspHI T'CATG_A
Cuts at: 4232 5962 4232
Size: 1730 4613
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2691 3376 3698 844
Size: 1847 685 322 3489
Fragments arranged by size:
3489 1847 685 322
BsrBI CCG'CTC
Cuts at: 1394 1823 2101 2763 3064 4230 1394
Size: 429 278 662 301 1166 3507
Fragments arranged by size:
3507 1166 662 429 301 278
BsrDI GCAATG_nn'
Cuts at: 65 1248 3445 4838 5012 65
Size: 1183 2197 1393 174 1396
Fragments arranged by size:
2197 1396 1393 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2314 3300 4987 5174 1700
Size: 110 504 986 1687 187 2869
Fragments arranged by size:
2869 1687 986 504 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6343
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6343
BssSI C'ACGA_G
Cuts at: 4403 5415 4403
Size: 1012 5331
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6343
BstBI TT'CG_AA
Cuts at: 5147 5147
Size: 6343
BstEII G'GTnAC_C
Cuts at: 1184 2821 1184
Size: 1637 4706
BstXI CCAn_nnnn'nTGG
Cuts at: 2506 2506
Size: 6343
BstYI r'GATC_y
Cuts at: 1972 2419 2436 3673 4040 4425 4442 5894 5980
Size: 447 17 1237 367 385 17 1452 86
Cuts at: 5980 5992 6338 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1237 447 385 367 346 86
17 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5791
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5604
Fragments arranged by size:
5604 683 56
BtsI GCAGTG_nn'
Cuts at: 3422 3859 4653 4673 3422
Size: 437 794 20 5092
Fragments arranged by size:
5092 794 437 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6343
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6308
DraI TTT'AAA
Cuts at: 3647 3647
Size: 6343
DraIII CAC_nnn'GTG
Cuts at: 2309 2309
Size: 6343
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5350 809
Size: 507 4034 1802
Fragments arranged by size:
4034 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3478 3790 4679
Size: 54 1425 50 275 18 1648 312 889
Cuts at: 4679 6308 8
Size: 1629 43
Fragments arranged by size:
1648 1629 1425 889 312 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3790 1537
Size: 275 1978 4090
Fragments arranged by size:
4090 1978 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2427 3054 4273 1353
Size: 1074 627 1219 3423
Fragments arranged by size:
3423 1219 1074 627
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3622 4930 5304 5450 387
Size: 1064 138 2033 1308 374 146 1280
Fragments arranged by size:
2033 1308 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2367 2624 3011 3173 3299 3395 3425
Size: 393 735 257 387 162 126 96 30
Cuts at: 3425 4406 5003 5790 6058 1239
Size: 981 597 787 268 1524
Fragments arranged by size:
1524 981 787 735 597 393 387 268
257 162 126 96 30
EcoNI CCTnn'n_nnAGG
Cuts at: 3095 3634 3095
Size: 539 5804
EcoO109I rG'GnC_Cy
Cuts at: 2081 2138 2202 2203 2284 2285 2995 3328 3379
Size: 57 64 1 81 1 710 333 51
Cuts at: 3379 3463 3524 2081
Size: 84 61 4900
Fragments arranged by size:
4900 710 333 84 81 64 61 57
51 1 1
EcoRI G'AATT_C
Cuts at: 2617 3652 4030 2617
Size: 1035 378 4930
Fragments arranged by size:
4930 1035 378
FspI TGC'GCA
Cuts at: 4849 4849
Size: 6343
HaeII r_GCGC'y
Cuts at: 1678 3569 5490 1678
Size: 1891 1921 2531
Fragments arranged by size:
2531 1921 1891
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3601 3633 4984 5016 5058
Size: 32 135 32 2029 32 1351 32 42
Cuts at: 5058 5090 1373
Size: 32 2626
Fragments arranged by size:
2626 2029 1351 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3687 3933 669
Size: 988 281 1749 246 3079
Fragments arranged by size:
3079 1749 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2723 748
Size: 1975 4368
HpaI GTT'AAC
Cuts at: 3933 3933
Size: 6343
KpnI G_GTAC'C
Cuts at: 3668 3668
Size: 6343
MfeI C'AATT_G
Cuts at: 3942 3942
Size: 6343
MluI A'CGCG_T
Cuts at: 6219 6219
Size: 6343
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2437 3421 5457 5641 1305
Size: 1132 984 2036 184 2007
Fragments arranged by size:
2036 2007 1132 984 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3480 10
Size: 54 1425 343 1648 2873
Fragments arranged by size:
2873 1648 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4301 4660 4819 518
Size: 648 3135 359 159 2042
Fragments arranged by size:
3135 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2662 2752 2788 2860 3010 3073 3759 4436
Size: 1056 90 36 72 150 63 686 677
Cuts at: 4436 5584 5829 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 1056 686 677 245 150 90
72 63 36
NaeI GCC'GGC
Cuts at: 2316 2316
Size: 6343
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5791
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6343
NgoMIV G'CCGG_C
Cuts at: 2314 2314
Size: 6343
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6343
NotI GC'GGCC_GC
Cuts at: 3790 3790
Size: 6343
NspI r_CATG'y
Cuts at: 1132 1229 3701 5246 1132
Size: 97 2472 1545 2229
Fragments arranged by size:
2472 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3552 1348
Size: 2204 4139
PciI A'CATG_T
Cuts at: 5242 5242
Size: 6343
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3403 1108
Size: 2295 4048
PfoI T'CCnGG_A
Cuts at: 2342 3360 2342
Size: 1018 5325
PmeI GTTT'AAAC
Cuts at: 3647 3647
Size: 6343
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4412 4444 5925 5957 4412
Size: 32 1481 32 4798
Fragments arranged by size:
4798 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2081 2138 2995 3463 2081
Size: 57 857 468 4961
Fragments arranged by size:
4961 857 468 57
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6343
PsiI TTA'TAA
Cuts at: 3913 3913
Size: 6343
PspOMI G'GGCC_C
Cuts at: 2202 2284 2790 2202
Size: 82 506 5755
Fragments arranged by size:
5755 506 82
PstI C_TGCA'G
Cuts at: 830 2125 2751 2865 3387 3429 3695 830
Size: 1295 626 114 522 42 266 3478
Fragments arranged by size:
3478 1295 626 522 266 114 42
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6343
PvuII CAG'CTG
Cuts at: 2662 2860 3010 2662
Size: 198 150 5995
Fragments arranged by size:
5995 198 150
SalI G'TCGA_C
Cuts at: 1936 3685 1936
Size: 1749 4594
SapI GCTCTTCn'nnn_
Cuts at: 3054 3054
Size: 6343
SbfI CC_TGCA'GG
Cuts at: 3387 3695 3387
Size: 308 6035
ScaI AGT'ACT
Cuts at: 4591 6303 4591
Size: 1712 4631
SfcI C'TryA_G
Cuts at: 826 1045 2121 2642 2747 2861 3383 3425 3691
Size: 219 1076 521 105 114 522 42 266
Cuts at: 3691 4826 5507 5698 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1076 681 522 521 266 219
191 114 105 42
SfiI GGCCn_nnn'nGGCC
Cuts at: 2711 3523 2711
Size: 812 5531
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6343
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6343
SmaI CCC'GGG
Cuts at: 1872 2795 3670 1872
Size: 923 875 4545
Fragments arranged by size:
4545 923 875
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2178 2726 2768 2930 3335 3534
Size: 197 926 235 548 42 162 405 199
Cuts at: 3534 3618 4447 5348 5610 820
Size: 84 829 901 262 1553
Fragments arranged by size:
1553 926 901 829 548 405 262 235
199 197 162 84 42
SnaBI TAC'GTA
Cuts at: 493 4134 493
Size: 3641 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6343
SphI G_CATG'C
Cuts at: 1132 3701 1132
Size: 2569 3774
SspI AAT'ATT
Cuts at: 5 52 4267 5
Size: 47 4215 2081
Fragments arranged by size:
4215 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6343
StyI C'CwwG_G
Cuts at: 513 1065 2412 2499 3782 513
Size: 552 1347 87 1283 3074
Fragments arranged by size:
3074 1347 1283 552 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2478 3556 4370 4387 4540 4725 5170
Size: 1255 898 1078 814 17 153 185 445
Cuts at: 5170 325
Size: 1498
Fragments arranged by size:
1498 1255 1078 898 814 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4589 6301 96
Size: 274 80 33 51 1443 2612 1712 138
Fragments arranged by size:
2612 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2747 2779 3398 3430 6303 6335 2747
Size: 32 619 32 2873 32 2755
Fragments arranged by size:
2873 2755 619 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2453 2978 1316
Size: 1137 525 4681
Fragments arranged by size:
4681 1137 525
XbaI T'CTAG_A
Cuts at: 3679 3679
Size: 6343
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6343
XmaI C'CCGG_G
Cuts at: 1870 2793 3668 1870
Size: 923 875 4545
Fragments arranged by size:
4545 923 875
XmnI GAAnn'nnTTC
Cuts at: 4472 4472
Size: 6343
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4151 276
Size: 53 83 186 936 2617 2468
Fragments arranged by size:
2617 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SapI SbfI ScaI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI
BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI
NarI NruI NsiI PacI PmlI PspXI PsrI RsrII
SacI SacII SanDI SexAI SfoI SrfI SwaI XcmI