Restriction map for
pFN21AB8695
(Circular) (Six-base) MAPSORT of: pFN21AB8695.seq Check: 9167 from: 1 to: 6865
pFN21AB8695 6865 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4675 278
Size: 53 83 186 936 3139 2468
Fragments arranged by size:
3139 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2140 4208 1937
Size: 203 2068 4594
Fragments arranged by size:
4594 2068 203
Acc65I G'GTAC_C
Cuts at: 4186 4186
Size: 6865
AclI AA'CG_TT
Cuts at: 1563 4992 5365 1563
Size: 3429 373 3063
Fragments arranged by size:
3429 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3032 3207 3248 4928 6312 6580 1632
Size: 1400 175 41 1680 1384 268 1917
Fragments arranged by size:
1917 1680 1400 1384 268 175 41
AflII C'TTAA_G
Cuts at: 820 1017 3626 820
Size: 197 2609 4059
Fragments arranged by size:
4059 2609 197
AflIII A'CryG_T
Cuts at: 1203 3677 5764 6741 1203
Size: 2474 2087 977 1327
Fragments arranged by size:
2474 2087 1327 977
AgeI A'CCGG_T
Cuts at: 5696 5696
Size: 6865
AhdI GACnn_n'nnGTC
Cuts at: 5594 5594
Size: 6865
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6865
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2218 1184
Size: 1034 5831
AlwNI CAG_nnn'CTG
Cuts at: 2756 2789 4608 6180 2756
Size: 33 1819 1572 3441
Fragments arranged by size:
3441 1819 1572 33
ApaLI G'TGCA_C
Cuts at: 4922 6078 4922
Size: 1156 5709
ApoI r'AATT_y
Cuts at: 1493 2406 2600 3179 3282 3333 4174 4400 4552
Size: 913 194 579 103 51 841 226 152
Cuts at: 4552 6661 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 913 841 579 226 194 152
103 51
AseI AT'TA_AT
Cuts at: 160 1032 2013 2637 4104 5419 160
Size: 872 981 624 1467 1315 1606
Fragments arranged by size:
1606 1467 1315 981 872 624
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6865
AvaI C'yCGr_G
Cuts at: 1870 1943 3093 4190 1870
Size: 73 1150 1097 4545
Fragments arranged by size:
4545 1150 1097 73
BamHI G'GATC_C
Cuts at: 3307 4195 4562 3307
Size: 888 367 5610
Fragments arranged by size:
5610 888 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3458 4186 5641 618
Size: 325 217 627 1671 728 1455 1842
Fragments arranged by size:
1842 1671 1455 728 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4184 1391
Size: 2793 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5894
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5056 5090 1903
Size: 34 3119 34 3678
Fragments arranged by size:
3678 3119 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3796 4757 5967 3796
Size: 961 1210 4694
Fragments arranged by size:
4694 1210 961
BclI T'GATC_A
Cuts at: 1548 2995 1548
Size: 1447 5418
BfrBI ATG'CAT
Cuts at: 3593 3739 3593
Size: 146 6719
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5476 136
Size: 107 122 71 631 605 3804 1525
Fragments arranged by size:
3804 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2744 6860 2744
Size: 4116 2749
BlpI GC'TnA_GC
Cuts at: 4282 4282
Size: 6865
Bme1580I G_kGCm'C
Cuts at: 1790 3797 4926 6082 1790
Size: 2007 1129 1156 2573
Fragments arranged by size:
2573 2007 1156 1129
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6865
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4596 5554 442
Size: 416 53 83 399 24 3179 958 1753
Fragments arranged by size:
3179 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6865
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2140 2733 3362 5525 1239
Size: 901 593 629 2163 2579
Fragments arranged by size:
2579 2163 901 629 593
Bpu10I CC'TnA_GC
Cuts at: 2109 2109
Size: 6865
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4990 5855 6153 4990
Size: 865 298 5702
Fragments arranged by size:
5702 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5528 882
Size: 617 4029 2219
Fragments arranged by size:
4029 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4656 493
Size: 711 3452 2702
Fragments arranged by size:
3452 2702 711
BsaBI GATnn'nnATC
Cuts at: 6859 6859
Size: 6865
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4672 5054 5689 275
Size: 53 83 186 936 3139 382 635 1451
Fragments arranged by size:
3139 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5298 5696 5970 6117 6528 5298
Size: 398 274 147 411 5635
Fragments arranged by size:
5635 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2257 2287 3189 3219 1178
Size: 30 165 30 854 30 902 30 4824
Fragments arranged by size:
4824 902 854 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3041 1186
Size: 1855 5010
BseYI C'CCAG_C
Cuts at: 6068 6564 6068
Size: 496 6369
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3630 1163
Size: 573 1894 4398
Fragments arranged by size:
4398 1894 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4315 5076 6104 1540
Size: 275 186 2314 761 1028 2301
Fragments arranged by size:
2314 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4926 5011 6082 4926
Size: 85 1071 5709
Fragments arranged by size:
5709 1071 85
BsmI GAATG_Cn'
Cuts at: 4376 4469 4376
Size: 93 6772
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3797 4184 4926 5011 6082 1391
Size: 399 2007 387 742 85 1071 2174
Fragments arranged by size:
2174 2007 1071 742 399 387 85
BspEI T'CCGG_A
Cuts at: 6528 6528
Size: 6865
BspHI T'CATG_A
Cuts at: 4754 6484 4754
Size: 1730 5135
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4220 844
Size: 3376 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4752 1394
Size: 429 2929 3507
Fragments arranged by size:
3507 2929 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5360 5534 65
Size: 1183 4112 174 1396
Fragments arranged by size:
4112 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5509 5696 1700
Size: 110 3699 187 2869
Fragments arranged by size:
3699 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2791 2896 96
Size: 2695 105 4065
Fragments arranged by size:
4065 2695 105
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6865
BssSI C'ACGA_G
Cuts at: 4925 5937 4925
Size: 1012 5853
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6865
BstBI TT'CG_AA
Cuts at: 5669 5669
Size: 6865
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6865
BstYI r'GATC_y
Cuts at: 1972 2744 2752 3307 3641 4195 4562 4947 4964
Size: 772 8 555 334 554 367 385 17
Cuts at: 4964 6416 6502 6514 6860 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 772 555 554 385 367 346
334 86 17 12 8
Bsu36I CC'TnA_GG
Cuts at: 3684 3684
Size: 6865
BtgI C'CryG_G
Cuts at: 513 1065 2233 2886 513
Size: 552 1168 653 4492
Fragments arranged by size:
4492 1168 653 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6126
Fragments arranged by size:
6126 683 56
BtsI GCAGTG_nn'
Cuts at: 4381 5175 5195 4381
Size: 794 20 6051
Fragments arranged by size:
6051 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6865
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2584 2619 3348 3383 529
Size: 35 2020 35 729 35 4011
Fragments arranged by size:
4011 2020 729 35 35 35
DraI TTT'AAA
Cuts at: 4169 4169
Size: 6865
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5872 809
Size: 507 4556 1802
Fragments arranged by size:
4556 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3758 4312 5201
Size: 54 1425 50 275 18 1928 554 889
Cuts at: 5201 6830 8
Size: 1629 43
Fragments arranged by size:
1928 1629 1425 889 554 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4312 1537
Size: 275 2500 4090
Fragments arranged by size:
4090 2500 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2514 2724 2973 3051 3267 4795 1353
Size: 1161 210 249 78 216 1528 3423
Fragments arranged by size:
3423 1528 1161 249 216 210 78
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5452 5826 5972 387
Size: 1064 138 3863 374 146 1280
Fragments arranged by size:
3863 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2140 2733 3032 3207 3248 3362 4928
Size: 393 508 593 299 175 41 114 1566
Cuts at: 4928 5525 6312 6580 1239
Size: 597 787 268 1524
Fragments arranged by size:
1566 1524 787 597 593 508 393 299
268 175 114 41
EcoO109I rG'GnC_Cy
Cuts at: 3090 3090
Size: 6865
EcoRI G'AATT_C
Cuts at: 3333 4174 4552 3333
Size: 841 378 5646
Fragments arranged by size:
5646 841 378
FspI TGC'GCA
Cuts at: 5371 5371
Size: 6865
HaeII r_GCGC'y
Cuts at: 1678 6012 1678
Size: 4334 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3154 3186 3189 3221 5506
Size: 32 135 32 1582 32 3 32 2285
Cuts at: 5506 5538 5580 5612 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2285 1582 135 42 32 32 32
32 32 32 3
HincII GTy'rAC
Cuts at: 669 1657 1938 4209 4455 669
Size: 988 281 2271 246 3079
Fragments arranged by size:
3079 2271 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2901 3841 748
Size: 2153 940 3772
Fragments arranged by size:
3772 2153 940
HpaI GTT'AAC
Cuts at: 4455 4455
Size: 6865
KpnI G_GTAC'C
Cuts at: 4190 4190
Size: 6865
MfeI C'AATT_G
Cuts at: 3342 4464 3342
Size: 1122 5743
MluI A'CGCG_T
Cuts at: 6741 6741
Size: 6865
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3544 3563 3993 5979 6163 1305
Size: 2239 19 430 1986 184 2007
Fragments arranged by size:
2239 2007 1986 430 184 19
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3760 10
Size: 54 1425 343 1928 3115
Fragments arranged by size:
3115 1928 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2303 4823 5182 5341 518
Size: 648 1137 2520 359 159 2042
Fragments arranged by size:
2520 2042 1137 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4006 4281 4958 6106 6351 1606
Size: 2400 275 677 1148 245 2120
Fragments arranged by size:
2400 2120 1148 677 275 245
NcoI C'CATG_G
Cuts at: 513 1065 2233 513
Size: 552 1168 5145
Fragments arranged by size:
5145 1168 552
NdeI CA'TA_TG
Cuts at: 387 3492 387
Size: 3105 3760
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6865
NotI GC'GGCC_GC
Cuts at: 4312 4312
Size: 6865
NsiI A_TGCA'T
Cuts at: 3595 3741 3595
Size: 146 6719
NspI r_CATG'y
Cuts at: 1132 1229 3681 4223 5768 1132
Size: 97 2452 542 1545 2229
Fragments arranged by size:
2452 2229 1545 542 97
PasI CC'CwG_GG
Cuts at: 1348 3790 1348
Size: 2442 4423
PciI A'CATG_T
Cuts at: 3677 5764 3677
Size: 2087 4778
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2756 1108
Size: 1648 5217
PfoI T'CCnGG_A
Cuts at: 2694 2694
Size: 6865
PmeI GTTT'AAAC
Cuts at: 4169 4169
Size: 6865
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4934 4966 6447 6479 4934
Size: 32 1481 32 5320
Fragments arranged by size:
5320 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3090 3090
Size: 6865
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6865
PsiI TTA'TAA
Cuts at: 2772 4435 2772
Size: 1663 5202
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2084 2116 2084
Size: 32 6833
PstI C_TGCA'G
Cuts at: 830 3274 4011 4098 4217 830
Size: 2444 737 87 119 3478
Fragments arranged by size:
3478 2444 737 119 87
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6865
PvuII CAG'CTG
Cuts at: 4006 4006
Size: 6865
SalI G'TCGA_C
Cuts at: 1936 4207 1936
Size: 2271 4594
SapI GCTCTTCn'nnn_
Cuts at: 2973 2973
Size: 6865
SbfI CC_TGCA'GG
Cuts at: 4217 4217
Size: 6865
ScaI AGT'ACT
Cuts at: 2323 5113 6825 2323
Size: 2790 1712 2363
Fragments arranged by size:
2790 2363 1712
SfcI C'TryA_G
Cuts at: 826 1045 2022 3270 3879 4007 4094 4213 5348
Size: 219 977 1248 609 128 87 119 1135
Cuts at: 5348 6029 6220 826
Size: 681 191 1471
Fragments arranged by size:
1471 1248 1135 977 681 609 219 191
128 119 87
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6865
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6865
SmaI CCC'GGG
Cuts at: 1872 3095 4192 1872
Size: 1223 1097 4545
Fragments arranged by size:
4545 1223 1097
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3626 4969 5870 6132 820
Size: 197 926 1683 1343 901 262 1553
Fragments arranged by size:
1683 1553 1343 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4656 493
Size: 4163 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6865
SphI G_CATG'C
Cuts at: 1132 4223 1132
Size: 3091 3774
SspI AAT'ATT
Cuts at: 5 52 4071 4789 5
Size: 47 4019 718 2081
Fragments arranged by size:
4019 2081 718 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6865
StyI C'CwwG_G
Cuts at: 513 1065 2233 2277 3498 4304 513
Size: 552 1168 44 1221 806 3074
Fragments arranged by size:
3074 1221 1168 806 552 44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4892 4909 5062 5247 5692 325
Size: 1255 3312 17 153 185 445 1498
Fragments arranged by size:
3312 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2321 2791 2896
Size: 274 80 33 51 1443 344 470 105
Cuts at: 2896 5111 6823 96
Size: 2215 1712 138
Fragments arranged by size:
2215 1712 1443 470 344 274 138 105
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2173 2205 2257 2289 3183 3215 6825 6857 2173
Size: 32 52 32 894 32 3610 32 2181
Fragments arranged by size:
3610 2181 894 52 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6865
XbaI T'CTAG_A
Cuts at: 3175 3973 4201 3175
Size: 798 228 5839
Fragments arranged by size:
5839 798 228
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6865
XmaI C'CCGG_G
Cuts at: 1870 3093 4190 1870
Size: 1223 1097 4545
Fragments arranged by size:
4545 1223 1097
XmnI GAAnn'nnTTC
Cuts at: 3261 3621 4143 4994 3261
Size: 360 522 851 5132
Fragments arranged by size:
5132 851 522 360
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4673 276
Size: 53 83 186 936 3139 2468
Fragments arranged by size:
3139 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PsrI PstI PvuI PvuII SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PmlI PspOMI PspXI RsrII SacI
SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI