Restriction map for
pFN21AB8694
(Circular) (Six-base) MAPSORT of: pFN21AB8694.seq Check: 9507 from: 1 to: 6070
pFN21AB8694 6070 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3880 278
Size: 53 83 186 936 2344 2468
Fragments arranged by size:
2468 2344 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2458 3413 1937
Size: 521 955 4594
Fragments arranged by size:
4594 955 521
Acc65I G'GTAC_C
Cuts at: 3391 3391
Size: 6070
AclI AA'CG_TT
Cuts at: 1563 4197 4570 1563
Size: 2634 373 3063
Fragments arranged by size:
3063 2634 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2633 2684 3237 4133 5517 5785 1632
Size: 1001 51 553 896 1384 268 1917
Fragments arranged by size:
1917 1384 1001 896 553 268 51
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5873
AflIII A'CryG_T
Cuts at: 1203 3208 4969 5946 1203
Size: 2005 1761 977 1327
Fragments arranged by size:
2005 1761 1327 977
AgeI A'CCGG_T
Cuts at: 4901 4901
Size: 6070
AhdI GACnn_n'nnGTC
Cuts at: 4799 4799
Size: 6070
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6070
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6070
AlwNI CAG_nnn'CTG
Cuts at: 2213 2820 3813 5385 2213
Size: 607 993 1572 2898
Fragments arranged by size:
2898 1572 993 607
ApaLI G'TGCA_C
Cuts at: 4127 5283 4127
Size: 1156 4914
ApoI r'AATT_y
Cuts at: 1493 2747 3379 3605 3757 5866 1493
Size: 1254 632 226 152 2109 1697
Fragments arranged by size:
2109 1697 1254 632 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4624 160
Size: 872 3592 1606
Fragments arranged by size:
3592 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6070
AvaI C'yCGr_G
Cuts at: 1870 1943 2015 2307 2592 3395 1870
Size: 73 72 292 285 803 4545
Fragments arranged by size:
4545 803 292 285 73 72
AvrII C'CTAG_G
Cuts at: 2911 2911
Size: 6070
BamHI G'GATC_C
Cuts at: 2915 3400 3767 2915
Size: 485 367 5218
Fragments arranged by size:
5218 485 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2011 2231 3391 4846 618
Size: 325 217 627 224 220 1160 1455 1842
Fragments arranged by size:
1842 1455 1160 627 325 224 220 217
BanII G_rGCy'C
Cuts at: 1391 2506 3389 1391
Size: 1115 883 4072
Fragments arranged by size:
4072 1115 883
BbeI G_GCGC'C
Cuts at: 2235 2235
Size: 6070
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5099
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4261 4295 1903
Size: 34 2324 34 3678
Fragments arranged by size:
3678 2324 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2749 3962 5172 2749
Size: 1213 1210 3647
Fragments arranged by size:
3647 1213 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6070
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4681 136
Size: 107 122 71 631 605 337 2672 1525
Fragments arranged by size:
2672 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 2325 6065 2325
Size: 3740 2330
BlpI GC'TnA_GC
Cuts at: 3487 3487
Size: 6070
Bme1580I G_kGCm'C
Cuts at: 1790 4131 5287 1790
Size: 2341 1156 2573
Fragments arranged by size:
2573 2341 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6070
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3801 4759 442
Size: 416 53 83 399 24 2384 958 1753
Fragments arranged by size:
2384 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6070
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2285 3309 3360 4730 1239
Size: 1046 1024 51 1370 2579
Fragments arranged by size:
2579 1370 1046 1024 51
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2518 4195 5060 5358 2518
Size: 1677 865 298 3230
Fragments arranged by size:
3230 1677 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2261 4733 882
Size: 617 762 2472 2219
Fragments arranged by size:
2472 2219 762 617
BsaAI yAC'GTr
Cuts at: 493 1204 3861 493
Size: 711 2657 2702
Fragments arranged by size:
2702 2657 711
BsaBI GATnn'nnATC
Cuts at: 6064 6064
Size: 6070
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2232 3877 4259 4894
Size: 53 83 186 936 699 1645 382 635
Cuts at: 4894 275
Size: 1451
Fragments arranged by size:
1645 1451 936 699 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3083 4503 4901 5175 5322 5733 3083
Size: 1420 398 274 147 411 3420
Fragments arranged by size:
3420 1420 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5845
Fragments arranged by size:
5845 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2078 1186
Size: 892 5178
BseYI C'CCAG_C
Cuts at: 2046 3074 5273 5769 2046
Size: 1028 2199 496 2347
Fragments arranged by size:
2347 2199 1028 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2387 1163
Size: 573 651 4846
Fragments arranged by size:
4846 651 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2037 2536 3520 4281 5309 1540
Size: 275 186 36 499 984 761 1028 2301
Fragments arranged by size:
2301 1028 984 761 499 275 186 36
BsiHKAI G_wGCw'C
Cuts at: 2506 4131 4216 5287 2506
Size: 1625 85 1071 3289
Fragments arranged by size:
3289 1625 1071 85
BsmI GAATG_Cn'
Cuts at: 3581 3674 3581
Size: 93 5977
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2506 3389 4131 4216 5287 1391
Size: 399 716 883 742 85 1071 2174
Fragments arranged by size:
2174 1071 883 742 716 399 85
BspEI T'CCGG_A
Cuts at: 3083 5733 3083
Size: 2650 3420
BspHI T'CATG_A
Cuts at: 3959 5689 3959
Size: 1730 4340
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3425 844
Size: 2581 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3957 1394
Size: 429 2134 3507
Fragments arranged by size:
3507 2134 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4565 4739 65
Size: 1183 3317 174 1396
Fragments arranged by size:
3317 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4714 4901 1700
Size: 110 2904 187 2869
Fragments arranged by size:
2904 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6070
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6070
BssSI C'ACGA_G
Cuts at: 4130 5142 4130
Size: 1012 5058
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6070
BstBI TT'CG_AA
Cuts at: 4874 4874
Size: 6070
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6070
BstYI r'GATC_y
Cuts at: 1972 2325 2574 2915 3400 3767 4152 4169 5621
Size: 353 249 341 485 367 385 17 1452
Cuts at: 5621 5707 5719 6065 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 485 385 367 353 346 341
249 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2884 513
Size: 552 939 880 3699
Fragments arranged by size:
3699 939 880 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5331
Fragments arranged by size:
5331 683 56
BtsI GCAGTG_nn'
Cuts at: 2258 2868 3586 4380 4400 2258
Size: 610 718 794 20 3928
Fragments arranged by size:
3928 794 718 610 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6070
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6035
DraI TTT'AAA
Cuts at: 2083 3374 2083
Size: 1291 4779
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2041 2895 5077 809
Size: 507 725 854 2182 1802
Fragments arranged by size:
2182 1802 854 725 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3517 4406 6035
Size: 54 1425 50 275 18 1687 889 1629
Cuts at: 6035 8
Size: 43
Fragments arranged by size:
1687 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3517 1537
Size: 275 1705 4090
Fragments arranged by size:
4090 1705 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2055 2618 3131 4000 1353
Size: 702 563 513 869 3423
Fragments arranged by size:
3423 869 702 563 513
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4657 5031 5177 387
Size: 1064 138 3068 374 146 1280
Fragments arranged by size:
3068 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2504 2504
Size: 6070
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2285 2633 2684 3237 3309 3360 4133
Size: 393 653 348 51 553 72 51 773
Cuts at: 4133 4730 5517 5785 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 787 773 653 597 553 393 348
268 72 51 51
EcoO109I rG'GnC_Cy
Cuts at: 2352 2352
Size: 6070
EcoRI G'AATT_C
Cuts at: 3379 3757 3379
Size: 378 5692
EcoRV GAT'ATC
Cuts at: 2194 2194
Size: 6070
FspI TGC'GCA
Cuts at: 4576 4576
Size: 6070
HaeII r_GCGC'y
Cuts at: 1678 2235 2911 3082 5217 1678
Size: 557 676 171 2135 2531
Fragments arranged by size:
2531 2135 676 557 171
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2387 2419 4711 4743 4785
Size: 32 135 32 815 32 2292 32 42
Cuts at: 4785 4817 1373
Size: 32 2626
Fragments arranged by size:
2626 2292 815 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2459 2569 3013 3414 3660 669
Size: 988 281 521 110 444 401 246 3079
Fragments arranged by size:
3079 988 521 444 401 281 246 110
HindIII A'AGCT_T
Cuts at: 748 2115 748
Size: 1367 4703
HpaI GTT'AAC
Cuts at: 3660 3660
Size: 6070
KasI G'GCGC_C
Cuts at: 2231 2231
Size: 6070
KpnI G_GTAC'C
Cuts at: 3395 3395
Size: 6070
MfeI C'AATT_G
Cuts at: 3669 3669
Size: 6070
MluI A'CGCG_T
Cuts at: 3208 5946 3208
Size: 2738 3332
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2057 3197 5184 5368 1305
Size: 752 1140 1987 184 2007
Fragments arranged by size:
2007 1987 1140 752 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4248
Fragments arranged by size:
4248 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3000 4028 4387 4546 518
Size: 648 1834 1028 359 159 2042
Fragments arranged by size:
2042 1834 1028 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3486 4163 5311 5556 1606
Size: 1880 677 1148 245 2120
Fragments arranged by size:
2120 1880 1148 677 245
NarI GG'CG_CC
Cuts at: 2232 2232
Size: 6070
NcoI C'CATG_G
Cuts at: 513 1065 2004 2884 513
Size: 552 939 880 3699
Fragments arranged by size:
3699 939 880 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6070
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6070
NotI GC'GGCC_GC
Cuts at: 3517 3517
Size: 6070
NruI TCG'CGA
Cuts at: 3277 3277
Size: 6070
NspI r_CATG'y
Cuts at: 1132 1229 3428 4973 1132
Size: 97 2199 1545 2229
Fragments arranged by size:
2229 2199 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2347 1348
Size: 999 5071
PciI A'CATG_T
Cuts at: 4969 4969
Size: 6070
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6070
PmeI GTTT'AAAC
Cuts at: 3374 3374
Size: 6070
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4139 4171 5652 5684 4139
Size: 32 1481 32 4525
Fragments arranged by size:
4525 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2352 2352
Size: 6070
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6070
PsiI TTA'TAA
Cuts at: 3640 3640
Size: 6070
PstI C_TGCA'G
Cuts at: 830 2816 3422 830
Size: 1986 606 3478
Fragments arranged by size:
3478 1986 606
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6070
SacI G_AGCT'C
Cuts at: 2506 2506
Size: 6070
SalI G'TCGA_C
Cuts at: 1936 2457 3412 1936
Size: 521 955 4594
Fragments arranged by size:
4594 955 521
SbfI CC_TGCA'GG
Cuts at: 3422 3422
Size: 6070
ScaI AGT'ACT
Cuts at: 4318 6030 4318
Size: 1712 4358
SexAI A'CCwGG_T
Cuts at: 2763 3163 2763
Size: 400 5670
SfcI C'TryA_G
Cuts at: 826 1045 2812 3418 4553 5234 5425 826
Size: 219 1767 606 1135 681 191 1471
Fragments arranged by size:
1767 1471 1135 681 606 219 191
SfoI GGC'GCC
Cuts at: 2233 2233
Size: 6070
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6070
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6070
SmaI CCC'GGG
Cuts at: 1872 3397 1872
Size: 1525 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2497 2592 4174 5075 5337 820
Size: 197 926 554 95 1582 901 262 1553
Fragments arranged by size:
1582 1553 926 901 554 262 197 95
SnaBI TAC'GTA
Cuts at: 493 3861 493
Size: 3368 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6070
SphI G_CATG'C
Cuts at: 1132 3428 1132
Size: 2296 3774
SspI AAT'ATT
Cuts at: 5 52 2726 3994 5
Size: 47 2674 1268 2081
Fragments arranged by size:
2674 2081 1268 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6070
StyI C'CwwG_G
Cuts at: 513 1065 2004 2884 2911 2937 3036 3509 513
Size: 552 939 880 27 26 99 473 3074
Fragments arranged by size:
3074 939 880 552 473 99 27 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2051 2081 2550 4097 4114 4267 4452
Size: 1255 471 30 469 1547 17 153 185
Cuts at: 4452 4897 325
Size: 445 1498
Fragments arranged by size:
1547 1498 1255 471 469 445 185 153
30 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4316 6028 96
Size: 274 80 33 51 1443 2339 1712 138
Fragments arranged by size:
2339 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3352 3384 6030 6062 3352
Size: 32 2646 32 3360
Fragments arranged by size:
3360 2646 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6070
XbaI T'CTAG_A
Cuts at: 3406 3406
Size: 6070
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2980 2980
Size: 6070
XhoI C'TCGA_G
Cuts at: 1943 2592 1943
Size: 649 5421
XmaI C'CCGG_G
Cuts at: 1870 3395 1870
Size: 1525 4545
XmnI GAAnn'nnTTC
Cuts at: 2166 2395 2489 2606 4199 2166
Size: 229 94 117 1593 4037
Fragments arranged by size:
4037 1593 229 117 94
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3878 276
Size: 53 83 186 936 2344 2468
Fragments arranged by size:
2468 2344 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NruI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SacI SalI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoNI FalI FseI FspAI NaeI NgoMIV NsiI PacI
PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacII
SanDI SapI SfiI SrfI SwaI