Restriction map for
pFN21AB8693
(Circular) (Six-base) MAPSORT of: pFN21AB8693.seq Check: 1872 from: 1 to: 6319
pFN21AB8693 6319 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2535 4129 278
Size: 53 83 186 936 999 1594 2468
Fragments arranged by size:
2468 1594 999 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3662 1937
Size: 1725 4594
Acc65I G'GTAC_C
Cuts at: 3640 3640
Size: 6319
AclI AA'CG_TT
Cuts at: 1563 4446 4819 1563
Size: 2883 373 3063
Fragments arranged by size:
3063 2883 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2043 2957 3098 3248 4382 5766 6034 1632
Size: 411 914 141 150 1134 1384 268 1917
Fragments arranged by size:
1917 1384 1134 914 411 268 150 141
AfeI AGC'GCT
Cuts at: 2717 2717
Size: 6319
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6122
AflIII A'CryG_T
Cuts at: 1203 2310 3069 3135 5218 6195 1203
Size: 1107 759 66 2083 977 1327
Fragments arranged by size:
2083 1327 1107 977 759 66
AgeI A'CCGG_T
Cuts at: 5150 5150
Size: 6319
AhdI GACnn_n'nnGTC
Cuts at: 2390 5048 2390
Size: 2658 3661
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6319
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6319
AlwNI CAG_nnn'CTG
Cuts at: 2083 4062 5634 2083
Size: 1979 1572 2768
Fragments arranged by size:
2768 1979 1572
ApaLI G'TGCA_C
Cuts at: 4376 5532 4376
Size: 1156 5163
ApoI r'AATT_y
Cuts at: 1493 2762 3628 3854 4006 6115 1493
Size: 1269 866 226 152 2109 1697
Fragments arranged by size:
2109 1697 1269 866 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4873 160
Size: 872 3841 1606
Fragments arranged by size:
3841 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6319
AvaI C'yCGr_G
Cuts at: 1870 1943 2016 3258 3644 1870
Size: 73 73 1242 386 4545
Fragments arranged by size:
4545 1242 386 73 73
BamHI G'GATC_C
Cuts at: 3649 4016 3649
Size: 367 5952
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3548 3640 5095 618
Size: 325 217 627 1761 92 1455 1842
Fragments arranged by size:
1842 1761 1455 627 325 217 92
BanII G_rGCy'C
Cuts at: 1391 2808 3063 3638 1391
Size: 1417 255 575 4072
Fragments arranged by size:
4072 1417 575 255
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3236 3288 928
Size: 971 1337 52 3959
Fragments arranged by size:
3959 1337 971 52
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4510 4544 1903
Size: 34 2573 34 3678
Fragments arranged by size:
3678 2573 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2760 4211 5421 2760
Size: 1451 1210 3658
Fragments arranged by size:
3658 1451 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6319
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2444 3494 4930
Size: 107 122 71 631 605 772 1050 1436
Cuts at: 4930 136
Size: 1525
Fragments arranged by size:
1525 1436 1050 772 631 605 122 107
71
BglII A'GATC_T
Cuts at: 6314 6314
Size: 6319
BlpI GC'TnA_GC
Cuts at: 3152 3484 3736 3152
Size: 332 252 5735
Fragments arranged by size:
5735 332 252
Bme1580I G_kGCm'C
Cuts at: 1790 3135 4380 5536 1790
Size: 1345 1245 1156 2573
Fragments arranged by size:
2573 1345 1245 1156
BmgBI CAC'GTC
Cuts at: 1318 3072 1318
Size: 1754 4565
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2393 3384 3531
Size: 416 53 83 399 24 976 991 147
Cuts at: 3531 4050 5008 442
Size: 519 958 1753
Fragments arranged by size:
1753 991 976 958 519 416 399 147
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6319
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2128 3235 3499 2128
Size: 1107 264 4948
Fragments arranged by size:
4948 1107 264
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2123 2210 2300 2908 3587 4979 1239
Size: 884 87 90 608 679 1392 2579
Fragments arranged by size:
2579 1392 884 679 608 90 87
Bpu10I CC'TnA_GC
Cuts at: 2473 2473
Size: 6319
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4444 5309 5607 4444
Size: 865 298 5156
Fragments arranged by size:
5156 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4982 882
Size: 617 3483 2219
Fragments arranged by size:
3483 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4110 493
Size: 711 2906 2702
Fragments arranged by size:
2906 2702 711
BsaBI GATnn'nnATC
Cuts at: 6313 6313
Size: 6319
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2532 4126 4508 5143
Size: 53 83 186 936 999 1594 382 635
Cuts at: 5143 275
Size: 1451
Fragments arranged by size:
1594 1451 999 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3545 3594 4752 5150 5424 5571 5982 3545
Size: 49 1158 398 274 147 411 3882
Fragments arranged by size:
3882 1158 411 398 274 147 49
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3232 3262 1178
Size: 30 165 30 1829 30 4235
Fragments arranged by size:
4235 1829 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2120 2555 2680 2726 3179 3227 3253 1186
Size: 934 435 125 46 453 48 26 4252
Fragments arranged by size:
4252 934 453 435 125 48 46 26
BseYI C'CCAG_C
Cuts at: 2083 2712 3003 3409 5522 6018 2083
Size: 629 291 406 2113 496 2384
Fragments arranged by size:
2384 2113 629 496 406 291
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2398 2523 1163
Size: 573 662 125 4959
Fragments arranged by size:
4959 662 573 125
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3769 4530 5558 1540
Size: 275 186 1768 761 1028 2301
Fragments arranged by size:
2301 1768 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2554 4380 4465 5536 2554
Size: 1826 85 1071 3337
Fragments arranged by size:
3337 1826 1071 85
BsmI GAATG_Cn'
Cuts at: 3830 3923 3830
Size: 93 6226
BsmBI CGTCTCn'nnnn_
Cuts at: 3292 3292
Size: 6319
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2554 2808 3063 3135 3638 4380 4465
Size: 399 764 254 255 72 503 742 85
Cuts at: 4465 5536 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 764 742 503 399 255 254
85 72
BspEI T'CCGG_A
Cuts at: 3594 5982 3594
Size: 2388 3931
BspHI T'CATG_A
Cuts at: 4208 5938 4208
Size: 1730 4589
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2421 3674 844
Size: 1577 1253 3489
Fragments arranged by size:
3489 1577 1253
BsrBI CCG'CTC
Cuts at: 1394 1823 4206 1394
Size: 429 2383 3507
Fragments arranged by size:
3507 2383 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3616 4814 4988 65
Size: 1183 2368 1198 174 1396
Fragments arranged by size:
2368 1396 1198 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2009 2150 4963 5150 1700
Size: 110 199 141 2813 187 2869
Fragments arranged by size:
2869 2813 199 187 141 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6319
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6319
BssSI C'ACGA_G
Cuts at: 4379 5391 4379
Size: 1012 5307
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2510 3478 794
Size: 1716 968 3635
Fragments arranged by size:
3635 1716 968
BstBI TT'CG_AA
Cuts at: 2557 5123 2557
Size: 2566 3753
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6319
BstYI r'GATC_y
Cuts at: 1972 2908 3090 3649 4016 4401 4418 5870 5956
Size: 936 182 559 367 385 17 1452 86
Cuts at: 5956 5968 6314 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 936 559 385 367 346 182
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2076 2076
Size: 6319
BtgI C'CryG_G
Cuts at: 513 1065 2004 3310 3396 3580 513
Size: 552 939 1306 86 184 3252
Fragments arranged by size:
3252 1306 939 552 184 86
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5580
Fragments arranged by size:
5580 683 56
BtsI GCAGTG_nn'
Cuts at: 3835 4629 4649 3835
Size: 794 20 5505
Fragments arranged by size:
5505 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6319
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3310 3345 529
Size: 35 2746 35 3503
Fragments arranged by size:
3503 2746 35 35
DraI TTT'AAA
Cuts at: 3623 3623
Size: 6319
DraIII CAC_nnn'GTG
Cuts at: 2272 2272
Size: 6319
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5326 809
Size: 507 4010 1802
Fragments arranged by size:
4010 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2200 2251
Size: 54 1425 50 275 18 177 193 51
Cuts at: 2251 2436 3766 4655 6284 8
Size: 185 1330 889 1629 43
Fragments arranged by size:
1629 1425 1330 889 275 193 185 177
54 51 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3766 1537
Size: 275 1954 4090
Fragments arranged by size:
4090 1954 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2062 2174 2559 3026 3131 3239 4249 1353
Size: 709 112 385 467 105 108 1010 3423
Fragments arranged by size:
3423 1010 709 467 385 112 108 105
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4906 5280 5426 387
Size: 1064 138 3317 374 146 1280
Fragments arranged by size:
3317 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2043 2123 2210 2300 2908 2957 3098
Size: 393 411 80 87 90 608 49 141
Cuts at: 3098 3248 3587 4382 4979 5766 6034 1239
Size: 150 339 795 597 787 268 1524
Fragments arranged by size:
1524 795 787 608 597 411 393 339
268 150 141 90 87 80 49
EcoNI CCTnn'n_nnAGG
Cuts at: 2411 2411
Size: 6319
EcoO109I rG'GnC_Cy
Cuts at: 2046 3559 2046
Size: 1513 4806
EcoRI G'AATT_C
Cuts at: 3628 4006 3628
Size: 378 5941
EcoRV GAT'ATC
Cuts at: 2245 2245
Size: 6319
FspI TGC'GCA
Cuts at: 4825 4825
Size: 6319
HaeII r_GCGC'y
Cuts at: 1678 2016 2719 3422 5466 1678
Size: 338 703 703 2044 2531
Fragments arranged by size:
2531 2044 703 703 338
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2376 2408 2857 2881 2889
Size: 32 135 32 804 32 449 24 8
Cuts at: 2889 2913 4960 4992 5034 5066 1373
Size: 24 2047 32 42 32 2626
Fragments arranged by size:
2626 2047 804 449 135 42 32 32
32 32 32 24 24 8
HincII GTy'rAC
Cuts at: 669 1657 1938 2959 3663 3909 669
Size: 988 281 1021 704 246 3079
Fragments arranged by size:
3079 1021 988 704 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6319
HpaI GTT'AAC
Cuts at: 2959 3909 2959
Size: 950 5369
KpnI G_GTAC'C
Cuts at: 3644 3644
Size: 6319
MfeI C'AATT_G
Cuts at: 3918 3918
Size: 6319
MluI A'CGCG_T
Cuts at: 6195 6195
Size: 6319
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3568 5433 5617 1305
Size: 2263 1865 184 2007
Fragments arranged by size:
2263 2007 1865 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2253 10
Size: 54 1425 343 421 4076
Fragments arranged by size:
4076 1425 421 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3290 4277 4636 4795 518
Size: 648 2124 987 359 159 2042
Fragments arranged by size:
2124 2042 987 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2033 2145 2976 3735 4412 5560 5805 1606
Size: 427 112 831 759 677 1148 245 2120
Fragments arranged by size:
2120 1148 831 759 677 427 245 112
NaeI GCC'GGC
Cuts at: 2011 2011
Size: 6319
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4828
Fragments arranged by size:
4828 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6319
NgoMIV G'CCGG_C
Cuts at: 2009 2009
Size: 6319
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6319
NotI GC'GGCC_GC
Cuts at: 3766 3766
Size: 6319
NspI r_CATG'y
Cuts at: 1132 1229 3139 3295 3677 5222 1132
Size: 97 1910 156 382 1545 2229
Fragments arranged by size:
2229 1910 1545 382 156 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6319
PciI A'CATG_T
Cuts at: 3135 5218 3135
Size: 2083 4236
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6319
PfoI T'CCnGG_A
Cuts at: 2114 2277 2114
Size: 163 6156
PmeI GTTT'AAAC
Cuts at: 3623 3623
Size: 6319
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4388 4420 5901 5933 4388
Size: 32 1481 32 4774
Fragments arranged by size:
4774 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2046 2046
Size: 6319
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6319
PsiI TTA'TAA
Cuts at: 3889 3889
Size: 6319
PstI C_TGCA'G
Cuts at: 830 3474 3671 830
Size: 2644 197 3478
Fragments arranged by size:
3478 2644 197
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6319
SalI G'TCGA_C
Cuts at: 1936 3661 1936
Size: 1725 4594
SapI GCTCTTCn'nnn_
Cuts at: 2559 2559
Size: 6319
SbfI CC_TGCA'GG
Cuts at: 3671 3671
Size: 6319
ScaI AGT'ACT
Cuts at: 4567 6279 4567
Size: 1712 4607
SfcI C'TryA_G
Cuts at: 826 1045 3470 3667 4802 5483 5674 826
Size: 219 2425 197 1135 681 191 1471
Fragments arranged by size:
2425 1471 1135 681 219 197 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6319
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6319
SmaI CCC'GGG
Cuts at: 1872 3260 3646 1872
Size: 1388 386 4545
Fragments arranged by size:
4545 1388 386
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4423 5324 5586 820
Size: 197 926 2480 901 262 1553
Fragments arranged by size:
2480 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4110 493
Size: 3617 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6319
SphI G_CATG'C
Cuts at: 1132 3295 3677 1132
Size: 2163 382 3774
Fragments arranged by size:
3774 2163 382
SspI AAT'ATT
Cuts at: 5 52 4243 5
Size: 47 4191 2081
Fragments arranged by size:
4191 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6319
StyI C'CwwG_G
Cuts at: 513 1065 2004 2133 3328 3615 3758 513
Size: 552 939 129 1195 287 143 3074
Fragments arranged by size:
3074 1195 939 552 287 143 129
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4346 4363 4516 4701 5146 325
Size: 1255 2766 17 153 185 445 1498
Fragments arranged by size:
2766 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4565 6277 96
Size: 274 80 33 51 1443 2588 1712 138
Fragments arranged by size:
2588 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6279 6311 6279
Size: 32 6287
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6319
XbaI T'CTAG_A
Cuts at: 3655 3655
Size: 6319
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6319
XmaI C'CCGG_G
Cuts at: 1870 3258 3644 1870
Size: 1388 386 4545
Fragments arranged by size:
4545 1388 386
XmnI GAAnn'nnTTC
Cuts at: 4448 4448
Size: 6319
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2533 4127 276
Size: 53 83 186 936 999 1594 2468
Fragments arranged by size:
2468 1594 999 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BstXI BstZ17I EcoICRI FalI FseI FspAI
KasI NarI NruI NsiI PacI PmlI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI
SfoI SrfI SwaI XcmI