Restriction map for
pFN21AB8689
(Circular) (Six-base) MAPSORT of: pFN21AB8689.seq Check: 6922 from: 1 to: 6055
pFN21AB8689 6055 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2288 2288
Size: 6055
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3865 278
Size: 53 83 186 936 2329 2468
Fragments arranged by size:
2468 2329 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3398 1937
Size: 1461 4594
Acc65I G'GTAC_C
Cuts at: 3376 3376
Size: 6055
AclI AA'CG_TT
Cuts at: 1563 4182 4555 1563
Size: 2619 373 3063
Fragments arranged by size:
3063 2619 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2801 4118 5502 5770 1632
Size: 1169 1317 1384 268 1917
Fragments arranged by size:
1917 1384 1317 1169 268
AfeI AGC'GCT
Cuts at: 2417 2417
Size: 6055
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5858
AflIII A'CryG_T
Cuts at: 1203 3192 4954 5931 1203
Size: 1989 1762 977 1327
Fragments arranged by size:
1989 1762 1327 977
AgeI A'CCGG_T
Cuts at: 3014 4886 3014
Size: 1872 4183
AhdI GACnn_n'nnGTC
Cuts at: 4784 4784
Size: 6055
AleI CACnn'nnGTG
Cuts at: 1166 2612 2846 1166
Size: 1446 234 4375
Fragments arranged by size:
4375 1446 234
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6055
AlwNI CAG_nnn'CTG
Cuts at: 3798 5370 3798
Size: 1572 4483
ApaLI G'TGCA_C
Cuts at: 2257 4112 5268 2257
Size: 1855 1156 3044
Fragments arranged by size:
3044 1855 1156
ApoI r'AATT_y
Cuts at: 1493 3364 3590 3742 5851 1493
Size: 1871 226 152 2109 1697
Fragments arranged by size:
2109 1871 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4609 160
Size: 872 3577 1606
Fragments arranged by size:
3577 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6055
AvaI C'yCGr_G
Cuts at: 1870 1943 2222 2429 3059 3255 3380 1870
Size: 73 279 207 630 196 125 4545
Fragments arranged by size:
4545 630 279 207 196 125 73
BamHI G'GATC_C
Cuts at: 3385 3752 3385
Size: 367 5688
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2558 2837 3011 3017 3314
Size: 325 217 627 771 279 174 6 297
Cuts at: 3314 3376 4831 618
Size: 62 1455 1842
Fragments arranged by size:
1842 1455 771 627 325 297 279 217
174 62 6
BanII G_rGCy'C
Cuts at: 1391 2721 3374 1391
Size: 1330 653 4072
Fragments arranged by size:
4072 1330 653
BbeI G_GCGC'C
Cuts at: 2562 3318 2562
Size: 756 5299
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3133 928
Size: 971 1234 3850
Fragments arranged by size:
3850 1234 971
BbvCI CC'TCA_GC
Cuts at: 3006 3006
Size: 6055
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2213 2247 2827 2861 4246
Size: 34 50 34 192 34 580 34 1385
Cuts at: 4246 4280 1903
Size: 34 3678
Fragments arranged by size:
3678 1385 580 192 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3947 5157 3947
Size: 1210 4845
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6055
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2862 2955 4666
Size: 107 122 71 631 605 1190 93 1711
Cuts at: 4666 136
Size: 1525
Fragments arranged by size:
1711 1525 1190 631 605 122 107 93
71
BglII A'GATC_T
Cuts at: 2433 6050 2433
Size: 3617 2438
BlpI GC'TnA_GC
Cuts at: 2626 3472 2626
Size: 846 5209
Bme1580I G_kGCm'C
Cuts at: 1790 2261 3014 3022 4116 5272 1790
Size: 471 753 8 1094 1156 2573
Fragments arranged by size:
2573 1156 1094 753 471 8
BmgBI CAC'GTC
Cuts at: 1318 2187 3166 1318
Size: 869 979 4207
Fragments arranged by size:
4207 979 869
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2932 3786 4744
Size: 416 53 83 399 24 1515 854 958
Cuts at: 4744 442
Size: 1753
Fragments arranged by size:
1753 1515 958 854 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6055
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2197 4715 1239
Size: 958 2518 2579
Fragments arranged by size:
2579 2518 958
Bpu10I CC'TnA_GC
Cuts at: 3006 3006
Size: 6055
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2141 4180 5045 5343 2141
Size: 2039 865 298 2853
Fragments arranged by size:
2853 2039 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2942 3056 4718 882
Size: 617 1443 114 1662 2219
Fragments arranged by size:
2219 1662 1443 617 114
BsaAI yAC'GTr
Cuts at: 493 1204 2812 3846 493
Size: 711 1608 1034 2702
Fragments arranged by size:
2702 1608 1034 711
BsaBI GATnn'nnATC
Cuts at: 6049 6049
Size: 6055
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2559 3315 3862 4244
Size: 53 83 186 936 1026 756 547 382
Cuts at: 4244 4879 275
Size: 635 1451
Fragments arranged by size:
1451 1026 936 756 635 547 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3014 4488 4886 5160 5307 5718 3014
Size: 1474 398 274 147 411 3351
Fragments arranged by size:
3351 1474 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2333 2363 3107 3137 1178
Size: 30 165 30 930 30 744 30 4096
Fragments arranged by size:
4096 930 744 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3140 1186
Size: 1954 4101
BseYI C'CCAG_C
Cuts at: 2203 5258 5754 2203
Size: 3055 496 2504
Fragments arranged by size:
3055 2504 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2253 2573 2615 3030 3221 1163
Size: 573 517 320 42 415 191 3997
Fragments arranged by size:
3997 573 517 415 320 191 42
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2310 2400 2533 2727 3505 4266
Size: 275 186 309 90 133 194 778 761
Cuts at: 4266 5294 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 778 761 309 275 194 186
133 90
BsiHKAI G_wGCw'C
Cuts at: 2261 2721 4116 4201 5272 2261
Size: 460 1395 85 1071 3044
Fragments arranged by size:
3044 1395 1071 460 85
BsmI GAATG_Cn'
Cuts at: 3566 3659 3566
Size: 93 5962
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2261 2721 3014 3022 3374 4116 4201
Size: 399 471 460 293 8 352 742 85
Cuts at: 4201 5272 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 471 460 399 352 293
85 8
BspEI T'CCGG_A
Cuts at: 5718 5718
Size: 6055
BspHI T'CATG_A
Cuts at: 2331 3944 5674 2331
Size: 1613 1730 2712
Fragments arranged by size:
2712 1730 1613
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2288 2552 2587 3270 3410 844
Size: 1444 264 35 683 140 3489
Fragments arranged by size:
3489 1444 683 264 140 35
BsrBI CCG'CTC
Cuts at: 1394 1823 2569 3328 3942 1394
Size: 429 746 759 614 3507
Fragments arranged by size:
3507 759 746 614 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4550 4724 65
Size: 1183 3302 174 1396
Fragments arranged by size:
3302 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2395 3014 3109 4699 4886 1700
Size: 110 585 619 95 1590 187 2869
Fragments arranged by size:
2869 1590 619 585 187 110 95
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6055
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6055
BssSI C'ACGA_G
Cuts at: 4115 5127 4115
Size: 1012 5043
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6055
BstBI TT'CG_AA
Cuts at: 4859 4859
Size: 6055
BstEII G'GTnAC_C
Cuts at: 1184 2288 2599 1184
Size: 1104 311 4640
Fragments arranged by size:
4640 1104 311
BstXI CCAn_nnnn'nTGG
Cuts at: 2848 2848
Size: 6055
BstYI r'GATC_y
Cuts at: 1972 2151 2433 2817 3385 3752 4137 4154 5606
Size: 179 282 384 568 367 385 17 1452
Cuts at: 5606 5692 5704 6050 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 568 385 384 367 346 282
179 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2516 2516
Size: 6055
BtgI C'CryG_G
Cuts at: 513 1065 2314 3084 3156 513
Size: 552 1249 770 72 3412
Fragments arranged by size:
3412 1249 770 552 72
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5316
Fragments arranged by size:
5316 683 56
BtsI GCAGTG_nn'
Cuts at: 2634 3571 4365 4385 2634
Size: 937 794 20 4304
Fragments arranged by size:
4304 937 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6055
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6020
DraI TTT'AAA
Cuts at: 3359 3359
Size: 6055
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5062 809
Size: 507 3746 1802
Fragments arranged by size:
3746 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2096 2307 2397
Size: 54 1425 50 275 18 266 211 90
Cuts at: 2397 2469 2564 2631 2724 3087 3180 3502 4391
Size: 72 95 67 93 363 93 322 889
Cuts at: 4391 6020 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 363 322 275 266 211
95 93 93 90 72 67 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2307 2397 2724 3502 1537
Size: 275 495 90 327 778 4090
Fragments arranged by size:
4090 778 495 327 275 90
EarI CTCTTCn'nnn_
Cuts at: 1353 2444 2504 3985 1353
Size: 1091 60 1481 3423
Fragments arranged by size:
3423 1481 1091 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2281 4642 5016 5162 387
Size: 1064 138 692 2361 374 146 1280
Fragments arranged by size:
2361 1280 1064 692 374 146 138
EcoICRI GAG'CTC
Cuts at: 2719 2719
Size: 6055
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2197 2801 4118 4715 5502 5770 1239
Size: 393 565 604 1317 597 787 268 1524
Fragments arranged by size:
1524 1317 787 604 597 565 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2326 2506 3118 3259 2326
Size: 180 612 141 5122
Fragments arranged by size:
5122 612 180 141
EcoRI G'AATT_C
Cuts at: 3364 3742 3364
Size: 378 5677
FspI TGC'GCA
Cuts at: 2243 2384 4561 2243
Size: 141 2177 3737
Fragments arranged by size:
3737 2177 141
HaeII r_GCGC'y
Cuts at: 1678 2184 2419 2562 3318 5202 1678
Size: 506 235 143 756 1884 2531
Fragments arranged by size:
2531 1884 756 506 235 143
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2783 2815 4696 4728 4770
Size: 32 135 32 1211 32 1881 32 42
Cuts at: 4770 4802 1373
Size: 32 2626
Fragments arranged by size:
2626 1881 1211 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3399 3645 669
Size: 988 281 1461 246 3079
Fragments arranged by size:
3079 1461 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6055
HpaI GTT'AAC
Cuts at: 3645 3645
Size: 6055
KasI G'GCGC_C
Cuts at: 2558 3314 2558
Size: 756 5299
KpnI G_GTAC'C
Cuts at: 3380 3380
Size: 6055
MfeI C'AATT_G
Cuts at: 3654 3654
Size: 6055
MluI A'CGCG_T
Cuts at: 3192 5931 3192
Size: 2739 3316
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3177 3246 5169 5353 1305
Size: 1872 69 1923 184 2007
Fragments arranged by size:
2007 1923 1872 184 69
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3089 10
Size: 54 1425 343 1257 2976
Fragments arranged by size:
2976 1425 1257 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2612 2846 2849 3000 3243 4013 4372
Size: 648 1446 234 3 151 243 770 359
Cuts at: 4372 4531 518
Size: 159 2042
Fragments arranged by size:
2042 1446 770 648 359 243 234 159
151 3
MspA1I CmG'CkG
Cuts at: 1606 2630 3471 4148 5296 5541 1606
Size: 1024 841 677 1148 245 2120
Fragments arranged by size:
2120 1148 1024 841 677 245
NarI GG'CG_CC
Cuts at: 2559 3315 2559
Size: 756 5299
NcoI C'CATG_G
Cuts at: 513 1065 2314 513
Size: 552 1249 4254
Fragments arranged by size:
4254 1249 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6055
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6055
NotI GC'GGCC_GC
Cuts at: 3502 3502
Size: 6055
NspI r_CATG'y
Cuts at: 1132 1229 2893 3413 4958 1132
Size: 97 1664 520 1545 2229
Fragments arranged by size:
2229 1664 1545 520 97
PasI CC'CwG_GG
Cuts at: 1348 2086 1348
Size: 738 5317
PciI A'CATG_T
Cuts at: 4954 4954
Size: 6055
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2812 1108
Size: 1704 4351
PfoI T'CCnGG_A
Cuts at: 2673 2673
Size: 6055
PmeI GTTT'AAAC
Cuts at: 3359 3359
Size: 6055
PmlI CAC'GTG
Cuts at: 2812 2812
Size: 6055
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4124 4156 5637 5669 4124
Size: 32 1481 32 4510
Fragments arranged by size:
4510 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2326 2506 3118 3259 2326
Size: 180 612 141 5122
Fragments arranged by size:
5122 612 180 141
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6055
PsiI TTA'TAA
Cuts at: 3625 3625
Size: 6055
PstI C_TGCA'G
Cuts at: 830 2123 3407 830
Size: 1293 1284 3478
Fragments arranged by size:
3478 1293 1284
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6055
SacI G_AGCT'C
Cuts at: 2721 2721
Size: 6055
SalI G'TCGA_C
Cuts at: 1936 3397 1936
Size: 1461 4594
SanDI GG'GwC_CC
Cuts at: 2506 2506
Size: 6055
SapI GCTCTTCn'nnn_
Cuts at: 2444 2444
Size: 6055
SbfI CC_TGCA'GG
Cuts at: 3407 3407
Size: 6055
ScaI AGT'ACT
Cuts at: 4303 6015 4303
Size: 1712 4343
SfcI C'TryA_G
Cuts at: 826 1045 2119 3403 4538 5219 5410 826
Size: 219 1074 1284 1135 681 191 1471
Fragments arranged by size:
1471 1284 1135 1074 681 219 191
SfoI GGC'GCC
Cuts at: 2560 3316 2560
Size: 756 5299
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6055
SgrAI Cr'CCGG_yG
Cuts at: 1700 3014 1700
Size: 1314 4741
SmaI CCC'GGG
Cuts at: 1872 3382 1872
Size: 1510 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2156 4159 5060 5322 820
Size: 197 926 213 2003 901 262 1553
Fragments arranged by size:
2003 1553 926 901 262 213 197
SnaBI TAC'GTA
Cuts at: 493 3846 493
Size: 3353 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6055
SphI G_CATG'C
Cuts at: 1132 2893 3413 1132
Size: 1761 520 3774
Fragments arranged by size:
3774 1761 520
SspI AAT'ATT
Cuts at: 5 52 3979 5
Size: 47 3927 2081
Fragments arranged by size:
3927 2081 47
StuI AGG'CCT
Cuts at: 1517 2990 3134 1517
Size: 1473 144 4438
Fragments arranged by size:
4438 1473 144
StyI C'CwwG_G
Cuts at: 513 1065 2015 2314 2352 3494 513
Size: 552 950 299 38 1142 3074
Fragments arranged by size:
3074 1142 950 552 299 38
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3064 4082 4099 4252 4437 4882 325
Size: 1255 1484 1018 17 153 185 445 1498
Fragments arranged by size:
1498 1484 1255 1018 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2829 3123 4301
Size: 274 80 33 51 1443 852 294 1178
Cuts at: 4301 6013 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1178 852 294 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2333 2365 6015 6047 2333
Size: 32 3650 32 2341
Fragments arranged by size:
3650 2341 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6055
XbaI T'CTAG_A
Cuts at: 3391 3391
Size: 6055
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6055
XmaI C'CCGG_G
Cuts at: 1870 3380 1870
Size: 1510 4545
XmnI GAAnn'nnTTC
Cuts at: 4184 4184
Size: 6055
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3863 276
Size: 53 83 186 936 2329 2468
Fragments arranged by size:
2468 2329 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI
PvuI SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI
PvuII RsrII SacII SexAI SfiI SrfI SwaI XcmI