Restriction map for
pFN21AB8688
(Circular) (Six-base) MAPSORT of: pFN21AB8688.seq Check: 4078 from: 1 to: 6211
pFN21AB8688 6211 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4021 278
Size: 53 83 186 936 2485 2468
Fragments arranged by size:
2485 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2110 3554 1937
Size: 173 1444 4594
Fragments arranged by size:
4594 1444 173
Acc65I G'GTAC_C
Cuts at: 3532 3532
Size: 6211
AclI AA'CG_TT
Cuts at: 1563 4338 4711 1563
Size: 2775 373 3063
Fragments arranged by size:
3063 2775 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2223 3371 4274 5658 5926 1632
Size: 591 1148 903 1384 268 1917
Fragments arranged by size:
1917 1384 1148 903 591 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6014
AflIII A'CryG_T
Cuts at: 1203 5110 6087 1203
Size: 3907 977 1327
Fragments arranged by size:
3907 1327 977
AgeI A'CCGG_T
Cuts at: 5042 5042
Size: 6211
AhdI GACnn_n'nnGTC
Cuts at: 4940 4940
Size: 6211
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6211
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3014 1184
Size: 1830 4381
AlwNI CAG_nnn'CTG
Cuts at: 3166 3954 5526 3166
Size: 788 1572 3851
Fragments arranged by size:
3851 1572 788
ApaLI G'TGCA_C
Cuts at: 3049 4268 5424 3049
Size: 1219 1156 3836
Fragments arranged by size:
3836 1219 1156
ApoI r'AATT_y
Cuts at: 1493 2084 2275 2902 3401 3520 3746 3898 6007
Size: 591 191 627 499 119 226 152 2109
Cuts at: 6007 1493
Size: 1697
Fragments arranged by size:
2109 1697 627 591 499 226 191 152
119
AseI AT'TA_AT
Cuts at: 160 1032 4765 160
Size: 872 3733 1606
Fragments arranged by size:
3733 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6211
AvaI C'yCGr_G
Cuts at: 1870 1943 2403 3495 3536 1870
Size: 73 460 1092 41 4545
Fragments arranged by size:
4545 1092 460 73 41
BamHI G'GATC_C
Cuts at: 3541 3908 3541
Size: 367 5844
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3532 4987 618
Size: 325 217 627 1745 1455 1842
Fragments arranged by size:
1842 1745 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2943 3530 1391
Size: 1552 587 4072
Fragments arranged by size:
4072 1552 587
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2499 2742 2846 3489 928
Size: 971 600 243 104 643 3650
Fragments arranged by size:
3650 971 643 600 243 104
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4402 4436 1903
Size: 34 2465 34 3678
Fragments arranged by size:
3678 2465 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4103 5313 4103
Size: 1210 5001
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6211
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2295 2852 4822
Size: 107 122 71 631 605 623 557 1970
Cuts at: 4822 136
Size: 1525
Fragments arranged by size:
1970 1525 631 623 605 557 122 107
71
BglII A'GATC_T
Cuts at: 6206 6206
Size: 6211
BlpI GC'TnA_GC
Cuts at: 3628 3628
Size: 6211
Bme1580I G_kGCm'C
Cuts at: 1790 3053 3123 4272 5428 1790
Size: 1263 70 1149 1156 2573
Fragments arranged by size:
2573 1263 1156 1149 70
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6211
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3942 4900 442
Size: 416 53 83 399 24 2525 958 1753
Fragments arranged by size:
2525 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6211
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2531 4871 1239
Size: 1292 2340 2579
Fragments arranged by size:
2579 2340 1292
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4336 5201 5499 4336
Size: 865 298 5048
Fragments arranged by size:
5048 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4874 882
Size: 617 3375 2219
Fragments arranged by size:
3375 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4002 493
Size: 711 2798 2702
Fragments arranged by size:
2798 2702 711
BsaBI GATnn'nnATC
Cuts at: 2466 6205 2466
Size: 3739 2472
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4018 4400 5035 275
Size: 53 83 186 936 2485 382 635 1451
Fragments arranged by size:
2485 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4644 5042 5316 5463 5874 4644
Size: 398 274 147 411 4981
Fragments arranged by size:
4981 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3495 3525 1178
Size: 30 165 30 2092 30 3864
Fragments arranged by size:
3864 2092 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2654 1186
Size: 1468 4743
BseYI C'CCAG_C
Cuts at: 3131 3173 5414 5910 3131
Size: 42 2241 496 3432
Fragments arranged by size:
3432 2241 496 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2046 1163
Size: 573 310 5328
Fragments arranged by size:
5328 573 310
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3661 4422 5450 1540
Size: 275 186 1660 761 1028 2301
Fragments arranged by size:
2301 1660 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2861 2943 3053 4272 4357 5428 2861
Size: 82 110 1219 85 1071 3644
Fragments arranged by size:
3644 1219 1071 110 85 82
BsiWI C'GTAC_G
Cuts at: 3219 3219
Size: 6211
BsmI GAATG_Cn'
Cuts at: 3722 3815 3722
Size: 93 6118
BsmBI CGTCTCn'nnnn_
Cuts at: 3188 3188
Size: 6211
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2861 2943 3053 3123 3530 4272 4357
Size: 399 1071 82 110 70 407 742 85
Cuts at: 4357 5428 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 1071 742 407 399 110 85
82 70
BspEI T'CCGG_A
Cuts at: 5874 5874
Size: 6211
BspHI T'CATG_A
Cuts at: 4100 5830 4100
Size: 1730 4481
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2134 2785 3566 844
Size: 1290 651 781 3489
Fragments arranged by size:
3489 1290 781 651
BsrBI CCG'CTC
Cuts at: 1394 1823 2233 3181 4098 1394
Size: 429 410 948 917 3507
Fragments arranged by size:
3507 948 917 429 410
BsrDI GCAATG_nn'
Cuts at: 65 1248 2893 4706 4880 65
Size: 1183 1645 1813 174 1396
Fragments arranged by size:
1813 1645 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4855 5042 1700
Size: 110 3045 187 2869
Fragments arranged by size:
3045 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6211
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6211
BssSI C'ACGA_G
Cuts at: 4271 5283 4271
Size: 1012 5199
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2877 794
Size: 2083 4128
BstBI TT'CG_AA
Cuts at: 5015 5015
Size: 6211
BstEII G'GTnAC_C
Cuts at: 1184 3088 1184
Size: 1904 4307
BstYI r'GATC_y
Cuts at: 1972 3541 3908 4293 4310 5762 5848 5860 6206
Size: 1569 367 385 17 1452 86 12 346
Cuts at: 6206 1972
Size: 1977
Fragments arranged by size:
1977 1569 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3084 513
Size: 552 939 1080 3640
Fragments arranged by size:
3640 1080 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5472
Fragments arranged by size:
5472 683 56
BtsI GCAGTG_nn'
Cuts at: 2935 3191 3727 4521 4541 2935
Size: 256 536 794 20 4605
Fragments arranged by size:
4605 794 536 256 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6211
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6176
DraI TTT'AAA
Cuts at: 3515 3515
Size: 6211
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5218 809
Size: 507 3902 1802
Fragments arranged by size:
3902 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3658 4547 6176
Size: 54 1425 50 275 18 1828 889 1629
Cuts at: 6176 8
Size: 43
Fragments arranged by size:
1828 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3658 1537
Size: 275 1846 4090
Fragments arranged by size:
4090 1846 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2199 2532 2691 3330 4141 1353
Size: 846 333 159 639 811 3423
Fragments arranged by size:
3423 846 811 639 333 159
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2474 4798 5172 5318 387
Size: 1064 138 885 2324 374 146 1280
Fragments arranged by size:
2324 1280 1064 885 374 146 138
EcoICRI GAG'CTC
Cuts at: 2941 2941
Size: 6211
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2223 2531 3371 4274 4871 5658 5926
Size: 393 591 308 840 903 597 787 268
Cuts at: 5926 1239
Size: 1524
Fragments arranged by size:
1524 903 840 787 597 591 393 308
268
EcoO109I rG'GnC_Cy
Cuts at: 2644 2644
Size: 6211
EcoRI G'AATT_C
Cuts at: 2275 2902 3520 3898 2275
Size: 627 618 378 4588
Fragments arranged by size:
4588 627 618 378
FspI TGC'GCA
Cuts at: 4717 4717
Size: 6211
HaeII r_GCGC'y
Cuts at: 1678 5358 1678
Size: 3680 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3047 3079 4852 4884 4926
Size: 32 135 32 1475 32 1773 32 42
Cuts at: 4926 4958 1373
Size: 32 2626
Fragments arranged by size:
2626 1773 1475 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3205 3555 3801 669
Size: 988 281 1267 350 246 3079
Fragments arranged by size:
3079 1267 988 350 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6211
HpaI GTT'AAC
Cuts at: 3801 3801
Size: 6211
KpnI G_GTAC'C
Cuts at: 3536 3536
Size: 6211
MfeI C'AATT_G
Cuts at: 3810 3810
Size: 6211
MluI A'CGCG_T
Cuts at: 6087 6087
Size: 6211
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5325 5509 1305
Size: 4020 184 2007
Fragments arranged by size:
4020 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4389
Fragments arranged by size:
4389 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2996 3459 4169 4528 4687 518
Size: 648 1830 463 710 359 159 2042
Fragments arranged by size:
2042 1830 710 648 463 359 159
MspA1I CmG'CkG
Cuts at: 1606 3135 3627 4304 5452 5697 1606
Size: 1529 492 677 1148 245 2120
Fragments arranged by size:
2120 1529 1148 677 492 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4720
Fragments arranged by size:
4720 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6211
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6211
NotI GC'GGCC_GC
Cuts at: 3658 3658
Size: 6211
NspI r_CATG'y
Cuts at: 1132 1229 3130 3569 5114 1132
Size: 97 1901 439 1545 2229
Fragments arranged by size:
2229 1901 1545 439 97
PasI CC'CwG_GG
Cuts at: 1348 2607 1348
Size: 1259 4952
PciI A'CATG_T
Cuts at: 5110 5110
Size: 6211
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2196 3077 1108
Size: 1088 881 4242
Fragments arranged by size:
4242 1088 881
PmeI GTTT'AAAC
Cuts at: 3515 3515
Size: 6211
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4280 4312 5793 5825 4280
Size: 32 1481 32 4666
Fragments arranged by size:
4666 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2644 2644
Size: 6211
PshAI GACnn'nnGTC
Cuts at: 1613 3200 1613
Size: 1587 4624
PsiI TTA'TAA
Cuts at: 2708 3781 2708
Size: 1073 5138
PstI C_TGCA'G
Cuts at: 830 2782 3162 3563 830
Size: 1952 380 401 3478
Fragments arranged by size:
3478 1952 401 380
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6211
PvuII CAG'CTG
Cuts at: 3135 3135
Size: 6211
SacI G_AGCT'C
Cuts at: 2943 2943
Size: 6211
SalI G'TCGA_C
Cuts at: 1936 3553 1936
Size: 1617 4594
SapI GCTCTTCn'nnn_
Cuts at: 2532 2532
Size: 6211
SbfI CC_TGCA'GG
Cuts at: 3563 3563
Size: 6211
ScaI AGT'ACT
Cuts at: 4459 6171 4459
Size: 1712 4499
SfcI C'TryA_G
Cuts at: 826 1045 2778 3158 3559 4694 5375 5566 826
Size: 219 1733 380 401 1135 681 191 1471
Fragments arranged by size:
1733 1471 1135 681 401 380 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6211
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6211
SmaI CCC'GGG
Cuts at: 1872 3538 1872
Size: 1666 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4315 5216 5478 820
Size: 197 926 2372 901 262 1553
Fragments arranged by size:
2372 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4002 493
Size: 3509 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6211
SphI G_CATG'C
Cuts at: 1132 3569 1132
Size: 2437 3774
SspI AAT'ATT
Cuts at: 5 52 2551 3262 4135 5
Size: 47 2499 711 873 2081
Fragments arranged by size:
2499 2081 873 711 47
StuI AGG'CCT
Cuts at: 1517 3146 1517
Size: 1629 4582
StyI C'CwwG_G
Cuts at: 513 1065 2004 2655 3650 513
Size: 552 939 651 995 3074
Fragments arranged by size:
3074 995 939 651 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2012 2936 4238 4255 4408 4593 5038
Size: 1255 432 924 1302 17 153 185 445
Cuts at: 5038 325
Size: 1498
Fragments arranged by size:
1498 1302 1255 924 445 432 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2158 2330 4457
Size: 274 80 33 51 1443 181 172 2127
Cuts at: 4457 6169 96
Size: 1712 138
Fragments arranged by size:
2127 1712 1443 274 181 172 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2228 2260 6171 6203 2228
Size: 32 3911 32 2236
Fragments arranged by size:
3911 2236 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6211
XbaI T'CTAG_A
Cuts at: 3547 3547
Size: 6211
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6211
XmaI C'CCGG_G
Cuts at: 1870 3536 1870
Size: 1666 4545
XmnI GAAnn'nnTTC
Cuts at: 3314 4340 3314
Size: 1026 5185
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4019 276
Size: 53 83 186 936 2485 2468
Fragments arranged by size:
2485 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BstXI BstZ17I Bsu36I DraIII
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI
SwaI XcmI