Restriction map for
pFN21AB8686
(Circular) (Six-base) MAPSORT of: pFN21AB8686.seq Check: 1072 from: 1 to: 5917
pFN21AB8686 5917 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3727 278
Size: 53 83 186 936 2191 2468
Fragments arranged by size:
2468 2191 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3260 1937
Size: 1323 4594
Acc65I G'GTAC_C
Cuts at: 3238 3238
Size: 5917
AclI AA'CG_TT
Cuts at: 1563 4044 4417 1563
Size: 2481 373 3063
Fragments arranged by size:
3063 2481 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3120 3980 5364 5632 1632
Size: 1488 860 1384 268 1917
Fragments arranged by size:
1917 1488 1384 860 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5720
AflIII A'CryG_T
Cuts at: 1203 2808 4816 5793 1203
Size: 1605 2008 977 1327
Fragments arranged by size:
2008 1605 1327 977
AgeI A'CCGG_T
Cuts at: 4748 4748
Size: 5917
AhdI GACnn_n'nnGTC
Cuts at: 4646 4646
Size: 5917
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5917
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5917
AlwNI CAG_nnn'CTG
Cuts at: 2047 3660 5232 2047
Size: 1613 1572 2732
Fragments arranged by size:
2732 1613 1572
ApaLI G'TGCA_C
Cuts at: 3974 5130 3974
Size: 1156 4761
ApoI r'AATT_y
Cuts at: 1493 2827 3070 3226 3452 3604 5713 1493
Size: 1334 243 156 226 152 2109 1697
Fragments arranged by size:
2109 1697 1334 243 226 156 152
AseI AT'TA_AT
Cuts at: 160 1032 2418 4471 160
Size: 872 1386 2053 1606
Fragments arranged by size:
2053 1606 1386 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5917
AvaI C'yCGr_G
Cuts at: 1870 1943 2086 3242 1870
Size: 73 143 1156 4545
Fragments arranged by size:
4545 1156 143 73
BamHI G'GATC_C
Cuts at: 3247 3614 3247
Size: 367 5550
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3238 4693 618
Size: 325 217 627 1451 1455 1842
Fragments arranged by size:
1842 1455 1451 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2138 3236 1391
Size: 747 1098 4072
Fragments arranged by size:
4072 1098 747
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2756 3195 928
Size: 971 857 439 3650
Fragments arranged by size:
3650 971 857 439
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4108 4142 1903
Size: 34 2171 34 3678
Fragments arranged by size:
3678 2171 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2849 3809 5019 2849
Size: 960 1210 3747
Fragments arranged by size:
3747 1210 960
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5917
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4528 136
Size: 107 122 71 631 605 2856 1525
Fragments arranged by size:
2856 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5912 5912
Size: 5917
BlpI GC'TnA_GC
Cuts at: 3334 3334
Size: 5917
Bme1580I G_kGCm'C
Cuts at: 1790 3978 5134 1790
Size: 2188 1156 2573
Fragments arranged by size:
2573 2188 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5917
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3648 4606 442
Size: 416 53 83 399 24 2231 958 1753
Fragments arranged by size:
2231 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5917
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2069 2688 4577 1239
Size: 830 619 1889 2579
Fragments arranged by size:
2579 1889 830 619
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4042 4907 5205 4042
Size: 865 298 4754
Fragments arranged by size:
4754 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4580 882
Size: 617 3081 2219
Fragments arranged by size:
3081 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3708 493
Size: 711 2504 2702
Fragments arranged by size:
2702 2504 711
BsaBI GATnn'nnATC
Cuts at: 2154 5911 2154
Size: 3757 2160
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3724 4106 4741 275
Size: 53 83 186 936 2191 382 635 1451
Fragments arranged by size:
2191 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4350 4748 5022 5169 5580 4350
Size: 398 274 147 411 4687
Fragments arranged by size:
4687 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5692
Fragments arranged by size:
5692 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2086 2104 1186
Size: 900 18 4999
Fragments arranged by size:
4999 900 18
BseYI C'CCAG_C
Cuts at: 2789 5120 5616 2789
Size: 2331 496 3090
Fragments arranged by size:
3090 2331 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2775 1163
Size: 573 1039 4305
Fragments arranged by size:
4305 1039 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2113 3367 4128 5156 1540
Size: 275 186 112 1254 761 1028 2301
Fragments arranged by size:
2301 1254 1028 761 275 186 112
BsiHKAI G_wGCw'C
Cuts at: 2138 2695 3978 4063 5134 2138
Size: 557 1283 85 1071 2921
Fragments arranged by size:
2921 1283 1071 557 85
BsmI GAATG_Cn'
Cuts at: 3428 3521 3428
Size: 93 5824
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2138 2695 3236 3978 4063 5134 1391
Size: 399 348 557 541 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 557 541 399 348 85
BspEI T'CCGG_A
Cuts at: 5580 5580
Size: 5917
BspHI T'CATG_A
Cuts at: 3806 5536 3806
Size: 1730 4187
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2071 3272 844
Size: 1227 1201 3489
Fragments arranged by size:
3489 1227 1201
BsrBI CCG'CTC
Cuts at: 1394 1823 2053 2094 3804 1394
Size: 429 230 41 1710 3507
Fragments arranged by size:
3507 1710 429 230 41
BsrDI GCAATG_nn'
Cuts at: 65 1248 2871 4412 4586 65
Size: 1183 1623 1541 174 1396
Fragments arranged by size:
1623 1541 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4561 4748 1700
Size: 110 2751 187 2869
Fragments arranged by size:
2869 2751 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5917
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5917
BssSI C'ACGA_G
Cuts at: 3977 4989 3977
Size: 1012 4905
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5917
BstBI TT'CG_AA
Cuts at: 2153 4721 2153
Size: 2568 3349
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5917
BstYI r'GATC_y
Cuts at: 1972 2793 3247 3614 3999 4016 5468 5554 5566
Size: 821 454 367 385 17 1452 86 12
Cuts at: 5566 5912 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 821 454 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4426
Fragments arranged by size:
4426 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5178
Fragments arranged by size:
5178 683 56
BtsI GCAGTG_nn'
Cuts at: 2966 3433 4227 4247 2966
Size: 467 794 20 4636
Fragments arranged by size:
4636 794 467 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5917
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5882
DraI TTT'AAA
Cuts at: 3221 3221
Size: 5917
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4924 809
Size: 507 3608 1802
Fragments arranged by size:
3608 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2110 3198 3364
Size: 54 1425 50 275 18 280 1088 166
Cuts at: 3364 4253 5882 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1088 889 280 275 166 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2110 3364 1537
Size: 275 298 1254 4090
Fragments arranged by size:
4090 1254 298 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2988 2994 3051 3847 1353
Size: 1635 6 57 796 3423
Fragments arranged by size:
3423 1635 796 57 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 4504 4878 5024 387
Size: 1064 138 435 2480 374 146 1280
Fragments arranged by size:
2480 1280 1064 435 374 146 138
EcoICRI GAG'CTC
Cuts at: 2136 2136
Size: 5917
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2069 2688 3120 3980 4577 5364 5632
Size: 393 437 619 432 860 597 787 268
Cuts at: 5632 1239
Size: 1524
Fragments arranged by size:
1524 860 787 619 597 437 432 393
268
EcoO109I rG'GnC_Cy
Cuts at: 2083 2083
Size: 5917
EcoRI G'AATT_C
Cuts at: 3226 3604 3226
Size: 378 5539
FspI TGC'GCA
Cuts at: 4423 4423
Size: 5917
HaeII r_GCGC'y
Cuts at: 1678 2026 5064 1678
Size: 348 3038 2531
Fragments arranged by size:
3038 2531 348
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3026 3058 4558 4590 4632
Size: 32 135 32 1454 32 1500 32 42
Cuts at: 4632 4664 1373
Size: 32 2626
Fragments arranged by size:
2626 1500 1454 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3159 3261 3507 669
Size: 988 281 1221 102 246 3079
Fragments arranged by size:
3079 1221 988 281 246 102
HindIII A'AGCT_T
Cuts at: 748 3181 748
Size: 2433 3484
HpaI GTT'AAC
Cuts at: 3159 3507 3159
Size: 348 5569
KpnI G_GTAC'C
Cuts at: 3242 3242
Size: 5917
MfeI C'AATT_G
Cuts at: 3516 3516
Size: 5917
MluI A'CGCG_T
Cuts at: 5793 5793
Size: 5917
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2383 5031 5215 1305
Size: 1078 2648 184 2007
Fragments arranged by size:
2648 2007 1078 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3200 10
Size: 54 1425 343 1368 2727
Fragments arranged by size:
2727 1425 1368 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3875 4234 4393 518
Size: 648 2709 359 159 2042
Fragments arranged by size:
2709 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2035 3333 4010 5158 5403 1606
Size: 429 1298 677 1148 245 2120
Fragments arranged by size:
2120 1298 1148 677 429 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4426
Fragments arranged by size:
4426 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5917
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5917
NotI GC'GGCC_GC
Cuts at: 3364 3364
Size: 5917
NspI r_CATG'y
Cuts at: 1132 1229 3275 4820 1132
Size: 97 2046 1545 2229
Fragments arranged by size:
2229 2046 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5917
PciI A'CATG_T
Cuts at: 4816 4816
Size: 5917
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2047 1108
Size: 939 4978
PfoI T'CCnGG_A
Cuts at: 2040 2665 2040
Size: 625 5292
PmeI GTTT'AAAC
Cuts at: 3221 3221
Size: 5917
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3986 4018 5499 5531 3986
Size: 32 1481 32 4372
Fragments arranged by size:
4372 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2083 2083
Size: 5917
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5917
PsiI TTA'TAA
Cuts at: 2660 3487 2660
Size: 827 5090
PstI C_TGCA'G
Cuts at: 830 2031 2082 3269 830
Size: 1201 51 1187 3478
Fragments arranged by size:
3478 1201 1187 51
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5917
SacI G_AGCT'C
Cuts at: 2138 2138
Size: 5917
SalI G'TCGA_C
Cuts at: 1936 3259 1936
Size: 1323 4594
SbfI CC_TGCA'GG
Cuts at: 3269 3269
Size: 5917
ScaI AGT'ACT
Cuts at: 4165 5877 4165
Size: 1712 4205
SfcI C'TryA_G
Cuts at: 826 1045 2027 2078 2161 3265 4400 5081 5272
Size: 219 982 51 83 1104 1135 681 191
Cuts at: 5272 826
Size: 1471
Fragments arranged by size:
1471 1135 1104 982 681 219 191 83
51
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5917
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5917
SmaI CCC'GGG
Cuts at: 1872 3244 1872
Size: 1372 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4021 4922 5184 820
Size: 197 926 2078 901 262 1553
Fragments arranged by size:
2078 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3708 493
Size: 3215 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5917
SphI G_CATG'C
Cuts at: 1132 3275 1132
Size: 2143 3774
SspI AAT'ATT
Cuts at: 5 52 2719 3841 5
Size: 47 2667 1122 2081
Fragments arranged by size:
2667 2081 1122 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5917
StyI C'CwwG_G
Cuts at: 513 1065 2004 2851 3356 513
Size: 552 939 847 505 3074
Fragments arranged by size:
3074 939 847 552 505
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2768 3944 3961 4114 4299 4744 325
Size: 1255 1188 1176 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1188 1176 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2440 2800 4163
Size: 274 80 33 51 1443 463 360 1363
Cuts at: 4163 5875 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1363 463 360 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3096 3128 5877 5909 3096
Size: 32 2749 32 3104
Fragments arranged by size:
3104 2749 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5917
XbaI T'CTAG_A
Cuts at: 3253 3253
Size: 5917
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2779 2779
Size: 5917
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5917
XmaI C'CCGG_G
Cuts at: 1870 3242 1870
Size: 1372 4545
XmnI GAAnn'nnTTC
Cuts at: 2472 4046 2472
Size: 1574 4343
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3725 276
Size: 53 83 186 936 2191 2468
Fragments arranged by size:
2468 2191 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI
PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI