Restriction map for
pFN21AB8684
(Circular) (Six-base) MAPSORT of: pFN21AB8684.seq Check: 4092 from: 1 to: 5842
pFN21AB8684 5842 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3652 278
Size: 53 83 186 936 2116 2468
Fragments arranged by size:
2468 2116 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3185 1937
Size: 1248 4594
Acc65I G'GTAC_C
Cuts at: 3163 3163
Size: 5842
AclI AA'CG_TT
Cuts at: 1563 2322 3969 4342 1563
Size: 759 1647 373 3063
Fragments arranged by size:
3063 1647 759 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2439 3905 5289 5557 1632
Size: 807 1466 1384 268 1917
Fragments arranged by size:
1917 1466 1384 807 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5645
AflIII A'CryG_T
Cuts at: 1203 2797 4741 5718 1203
Size: 1594 1944 977 1327
Fragments arranged by size:
1944 1594 1327 977
AgeI A'CCGG_T
Cuts at: 4673 4673
Size: 5842
AhdI GACnn_n'nnGTC
Cuts at: 2270 4571 2270
Size: 2301 3541
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5842
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2325 1184
Size: 1141 4701
AlwNI CAG_nnn'CTG
Cuts at: 2085 3585 5157 2085
Size: 1500 1572 2770
Fragments arranged by size:
2770 1572 1500
ApaLI G'TGCA_C
Cuts at: 3899 5055 3899
Size: 1156 4686
ApoI r'AATT_y
Cuts at: 1493 2342 2927 3151 3377 3529 5638 1493
Size: 849 585 224 226 152 2109 1697
Fragments arranged by size:
2109 1697 849 585 226 224 152
AseI AT'TA_AT
Cuts at: 160 1032 2255 4396 160
Size: 872 1223 2141 1606
Fragments arranged by size:
2141 1606 1223 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5842
AvaI C'yCGr_G
Cuts at: 1870 1943 3167 1870
Size: 73 1224 4545
Fragments arranged by size:
4545 1224 73
BamHI G'GATC_C
Cuts at: 3172 3539 3172
Size: 367 5475
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3163 4618 618
Size: 325 217 627 1376 1455 1842
Fragments arranged by size:
1842 1455 1376 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3161 1391
Size: 1770 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2907 928
Size: 971 1008 3863
Fragments arranged by size:
3863 1008 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4033 4067 1903
Size: 34 2096 34 3678
Fragments arranged by size:
3678 2096 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3734 4944 3734
Size: 1210 4632
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5842
BfrBI ATG'CAT
Cuts at: 2027 2157 2027
Size: 130 5712
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4453 136
Size: 107 122 71 631 605 2781 1525
Fragments arranged by size:
2781 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5837 5837
Size: 5842
BlpI GC'TnA_GC
Cuts at: 3259 3259
Size: 5842
Bme1580I G_kGCm'C
Cuts at: 1790 3903 5059 1790
Size: 2113 1156 2573
Fragments arranged by size:
2573 2113 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5842
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2468 3573 4531
Size: 416 53 83 399 24 1051 1105 958
Cuts at: 4531 442
Size: 1753
Fragments arranged by size:
1753 1105 1051 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5842
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4502 1239
Size: 3263 2579
Bpu10I CC'TnA_GC
Cuts at: 2999 2999
Size: 5842
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2632 2717 3967 4832 5130 2632
Size: 85 1250 865 298 3344
Fragments arranged by size:
3344 1250 865 298 85
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2257 4505 882
Size: 617 758 2248 2219
Fragments arranged by size:
2248 2219 758 617
BsaAI yAC'GTr
Cuts at: 493 1204 2149 3633 493
Size: 711 945 1484 2702
Fragments arranged by size:
2702 1484 945 711
BsaBI GATnn'nnATC
Cuts at: 3042 5836 3042
Size: 2794 3048
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2532 3649 4031 4666
Size: 53 83 186 936 999 1117 382 635
Cuts at: 4666 275
Size: 1451
Fragments arranged by size:
1451 1117 999 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4275 4673 4947 5094 5505 4275
Size: 398 274 147 411 4612
Fragments arranged by size:
4612 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2451 2481 1178
Size: 30 165 30 1048 30 4539
Fragments arranged by size:
4539 1048 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2761 1186
Size: 1575 4267
BseYI C'CCAG_C
Cuts at: 5045 5541 5045
Size: 496 5346
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5269
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3292 4053 5081 1540
Size: 275 186 1291 761 1028 2301
Fragments arranged by size:
2301 1291 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3903 3988 5059 3903
Size: 85 1071 4686
Fragments arranged by size:
4686 1071 85
BsmI GAATG_Cn'
Cuts at: 2096 3353 3446 2096
Size: 1257 93 4492
Fragments arranged by size:
4492 1257 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3161 3903 3988 5059 1391
Size: 399 1371 742 85 1071 2174
Fragments arranged by size:
2174 1371 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5505 5505
Size: 5842
BspHI T'CATG_A
Cuts at: 3731 5461 3731
Size: 1730 4112
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3197 844
Size: 2353 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3729 1394
Size: 429 1906 3507
Fragments arranged by size:
3507 1906 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2029 4337 4511 65
Size: 1183 781 2308 174 1396
Fragments arranged by size:
2308 1396 1183 781 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4486 4673 1700
Size: 110 2676 187 2869
Fragments arranged by size:
2869 2676 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5842
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5842
BssSI C'ACGA_G
Cuts at: 3902 4914 3902
Size: 1012 4830
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2109 794
Size: 1315 4527
BstBI TT'CG_AA
Cuts at: 4646 4646
Size: 5842
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5842
BstYI r'GATC_y
Cuts at: 1972 3050 3172 3539 3924 3941 5393 5479 5491
Size: 1078 122 367 385 17 1452 86 12
Cuts at: 5491 5837 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1078 385 367 346 122 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2721 2883 2721
Size: 162 5680
BtgI C'CryG_G
Cuts at: 513 1065 2004 2535 513
Size: 552 939 531 3820
Fragments arranged by size:
3820 939 552 531
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2145 487
Size: 683 56 919 4184
Fragments arranged by size:
4184 919 683 56
BtsI GCAGTG_nn'
Cuts at: 3358 4152 4172 3358
Size: 794 20 5028
Fragments arranged by size:
5028 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5842
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2011 2046 529
Size: 35 1447 35 4325
Fragments arranged by size:
4325 1447 35 35
DraI TTT'AAA
Cuts at: 2370 3146 2370
Size: 776 5066
DraIII CAC_nnn'GTG
Cuts at: 2476 2476
Size: 5842
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4849 809
Size: 507 3533 1802
Fragments arranged by size:
3533 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2462 3289 4178
Size: 54 1425 50 275 18 632 827 889
Cuts at: 4178 5807 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 827 632 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3289 1537
Size: 275 1477 4090
Fragments arranged by size:
4090 1477 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2738 2784 2807 3051 3772 1353
Size: 1385 46 23 244 721 3423
Fragments arranged by size:
3423 1385 721 244 46 23
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2904 4429 4803 4949 387
Size: 1064 138 1315 1525 374 146 1280
Fragments arranged by size:
1525 1315 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2439 3905 4502 5289 5557 1239
Size: 393 807 1466 597 787 268 1524
Fragments arranged by size:
1524 1466 807 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2919 2919
Size: 5842
EcoO109I rG'GnC_Cy
Cuts at: 2968 2968
Size: 5842
EcoRI G'AATT_C
Cuts at: 3151 3529 3151
Size: 378 5464
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3149 3149
Size: 5842
FspI TGC'GCA
Cuts at: 4348 4348
Size: 5842
HaeII r_GCGC'y
Cuts at: 1678 2781 4989 1678
Size: 1103 2208 2531
Fragments arranged by size:
2531 2208 1103
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2256 2288 4483 4515 4557
Size: 32 135 32 684 32 2195 32 42
Cuts at: 4557 4589 1373
Size: 32 2626
Fragments arranged by size:
2626 2195 684 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3186 3432 669
Size: 988 281 1248 246 3079
Fragments arranged by size:
3079 1248 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2421 3131 748
Size: 1673 710 3459
Fragments arranged by size:
3459 1673 710
HpaI GTT'AAC
Cuts at: 3432 3432
Size: 5842
KpnI G_GTAC'C
Cuts at: 3167 3167
Size: 5842
MfeI C'AATT_G
Cuts at: 3441 3441
Size: 5842
MluI A'CGCG_T
Cuts at: 2797 5718 2797
Size: 2921 2921
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2451 4956 5140 1305
Size: 1146 2505 184 2007
Fragments arranged by size:
2505 2007 1146 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4020
Fragments arranged by size:
4020 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3800 4159 4318 518
Size: 648 2634 359 159 2042
Fragments arranged by size:
2634 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3258 3935 5083 5328 1606
Size: 1652 677 1148 245 2120
Fragments arranged by size:
2120 1652 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4351
Fragments arranged by size:
4351 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5842
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5842
NotI GC'GGCC_GC
Cuts at: 3289 3289
Size: 5842
NsiI A_TGCA'T
Cuts at: 2029 2159 2029
Size: 130 5712
NspI r_CATG'y
Cuts at: 1132 1229 2022 2031 2069 2492 3200 4745 1132
Size: 97 793 9 38 423 708 1545 2229
Fragments arranged by size:
2229 1545 793 708 423 97 38 9
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5842
PciI A'CATG_T
Cuts at: 4741 4741
Size: 5842
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2476 1108
Size: 1368 4474
PmeI GTTT'AAAC
Cuts at: 3146 3146
Size: 5842
PmlI CAC'GTG
Cuts at: 2149 2149
Size: 5842
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3911 3943 5424 5456 3911
Size: 32 1481 32 4297
Fragments arranged by size:
4297 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5842
PsiI TTA'TAA
Cuts at: 3412 3412
Size: 5842
PstI C_TGCA'G
Cuts at: 830 2740 3194 830
Size: 1910 454 3478
Fragments arranged by size:
3478 1910 454
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5842
SalI G'TCGA_C
Cuts at: 1936 3184 1936
Size: 1248 4594
SbfI CC_TGCA'GG
Cuts at: 3194 3194
Size: 5842
ScaI AGT'ACT
Cuts at: 3023 4090 5802 3023
Size: 1067 1712 3063
Fragments arranged by size:
3063 1712 1067
SexAI A'CCwGG_T
Cuts at: 2397 2397
Size: 5842
SfcI C'TryA_G
Cuts at: 826 1045 2736 3190 4325 5006 5197 826
Size: 219 1691 454 1135 681 191 1471
Fragments arranged by size:
1691 1471 1135 681 454 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5842
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5842
SmaI CCC'GGG
Cuts at: 1872 3169 1872
Size: 1297 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2647 2696 3946 4847 5109 820
Size: 197 926 704 49 1250 901 262 1553
Fragments arranged by size:
1553 1250 926 901 704 262 197 49
SnaBI TAC'GTA
Cuts at: 493 3633 493
Size: 3140 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5842
SphI G_CATG'C
Cuts at: 1132 3200 1132
Size: 2068 3774
SspI AAT'ATT
Cuts at: 5 52 3766 5
Size: 47 3714 2081
Fragments arranged by size:
3714 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5842
StyI C'CwwG_G
Cuts at: 513 1065 2004 2505 3281 513
Size: 552 939 501 776 3074
Fragments arranged by size:
3074 939 776 552 501
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2487 3869 3886 4039 4224 4669 325
Size: 1255 907 1382 17 153 185 445 1498
Fragments arranged by size:
1498 1382 1255 907 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3021 4088 5800
Size: 274 80 33 51 1443 1044 1067 1712
Cuts at: 5800 96
Size: 138
Fragments arranged by size:
1712 1443 1067 1044 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2387 2419 2494 2526 5802 5834 2387
Size: 32 75 32 3276 32 2395
Fragments arranged by size:
3276 2395 75 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3125 1316
Size: 1809 4033
XbaI T'CTAG_A
Cuts at: 3178 3178
Size: 5842
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5842
XmaI C'CCGG_G
Cuts at: 1870 3167 1870
Size: 1297 4545
XmnI GAAnn'nnTTC
Cuts at: 2388 3971 2388
Size: 1583 4259
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3650 276
Size: 53 83 186 936 2116 2468
Fragments arranged by size:
2468 2116 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI