Restriction map for
pFN21AB8681
(Circular) (Six-base) MAPSORT of: pFN21AB8681.seq Check: 656 from: 1 to: 5836
pFN21AB8681 5836 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2250 2250
Size: 5836
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3646 278
Size: 53 83 186 936 2110 2468
Fragments arranged by size:
2468 2110 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3179 1937
Size: 1242 4594
Acc65I G'GTAC_C
Cuts at: 3090 3157 3090
Size: 67 5769
AclI AA'CG_TT
Cuts at: 1563 3963 4336 1563
Size: 2400 373 3063
Fragments arranged by size:
3063 2400 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3899 5283 5551 1632
Size: 2267 1384 268 1917
Fragments arranged by size:
2267 1917 1384 268
AfeI AGC'GCT
Cuts at: 2270 2270
Size: 5836
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5639
AflIII A'CryG_T
Cuts at: 1203 2330 4735 5712 1203
Size: 1127 2405 977 1327
Fragments arranged by size:
2405 1327 1127 977
AgeI A'CCGG_T
Cuts at: 4667 4667
Size: 5836
AhdI GACnn_n'nnGTC
Cuts at: 4565 4565
Size: 5836
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5836
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5836
AlwNI CAG_nnn'CTG
Cuts at: 2089 2548 3579 5151 2089
Size: 459 1031 1572 2774
Fragments arranged by size:
2774 1572 1031 459
ApaLI G'TGCA_C
Cuts at: 2732 3893 5049 2732
Size: 1161 1156 3519
Fragments arranged by size:
3519 1161 1156
ApoI r'AATT_y
Cuts at: 1493 3145 3371 3523 5632 1493
Size: 1652 226 152 2109 1697
Fragments arranged by size:
2109 1697 1652 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4390 160
Size: 872 3358 1606
Fragments arranged by size:
3358 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5836
AvaI C'yCGr_G
Cuts at: 1870 1943 2137 3161 1870
Size: 73 194 1024 4545
Fragments arranged by size:
4545 1024 194 73
BamHI G'GATC_C
Cuts at: 3166 3533 3166
Size: 367 5469
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2238 2446 2828 2892 3090
Size: 325 217 627 451 208 382 64 198
Cuts at: 3090 3157 4612 618
Size: 67 1455 1842
Fragments arranged by size:
1842 1455 627 451 382 325 217 208
198 67 64
BanII G_rGCy'C
Cuts at: 1391 2144 2171 2212 3155 1391
Size: 753 27 41 943 4072
Fragments arranged by size:
4072 943 753 41 27
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4865
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2354 2388 4027 4061 1903
Size: 34 417 34 1639 34 3678
Fragments arranged by size:
3678 1639 417 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3728 4938 3728
Size: 1210 4626
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5836
BfrBI ATG'CAT
Cuts at: 3050 3050
Size: 5836
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2112 2582 4447
Size: 107 122 71 631 605 440 470 1865
Cuts at: 4447 136
Size: 1525
Fragments arranged by size:
1865 1525 631 605 470 440 122 107
71
BglII A'GATC_T
Cuts at: 5831 5831
Size: 5836
BlpI GC'TnA_GC
Cuts at: 2272 3253 2272
Size: 981 4855
Bme1580I G_kGCm'C
Cuts at: 1790 2451 2736 3897 5053 1790
Size: 661 285 1161 1156 2573
Fragments arranged by size:
2573 1161 1156 661 285
BmgBI CAC'GTC
Cuts at: 1318 2331 1318
Size: 1013 4823
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3567 4525 442
Size: 416 53 83 399 24 2150 958 1753
Fragments arranged by size:
2150 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5836
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2378 2378
Size: 5836
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2083 2885 4496 1239
Size: 844 802 1611 2579
Fragments arranged by size:
2579 1611 844 802
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2504 2867 3961 4826 5124 2504
Size: 363 1094 865 298 3216
Fragments arranged by size:
3216 1094 865 363 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4499 882
Size: 617 3000 2219
Fragments arranged by size:
3000 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3627 493
Size: 711 2423 2702
Fragments arranged by size:
2702 2423 711
BsaBI GATnn'nnATC
Cuts at: 5830 5830
Size: 5836
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2780 3643 4025 4660
Size: 53 83 186 936 1247 863 382 635
Cuts at: 4660 275
Size: 1451
Fragments arranged by size:
1451 1247 936 863 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4269 4667 4941 5088 5499 4269
Size: 398 274 147 411 4606
Fragments arranged by size:
4606 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2687 2717 2726 2756 1178
Size: 30 165 30 1284 30 9 30 4258
Fragments arranged by size:
4258 1284 165 30 30 30 30 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2422 2488 2708 1186
Size: 1236 66 220 4314
Fragments arranged by size:
4314 1236 220 66
BseYI C'CCAG_C
Cuts at: 2068 2082 2179 2572 2658 3127 5039 5535 2068
Size: 14 97 393 86 469 1912 496 2369
Fragments arranged by size:
2369 1912 496 469 393 97 86 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2871 1163
Size: 573 1135 4128
Fragments arranged by size:
4128 1135 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3286 4047 5075 1540
Size: 275 186 1285 761 1028 2301
Fragments arranged by size:
2301 1285 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2736 3897 3982 5053 2736
Size: 1161 85 1071 3519
Fragments arranged by size:
3519 1161 1071 85
BsmI GAATG_Cn'
Cuts at: 3050 3347 3440 3050
Size: 297 93 5446
Fragments arranged by size:
5446 297 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2144 2171 2212 2451 2736 3155 3897
Size: 399 354 27 41 239 285 419 742
Cuts at: 3897 3982 5053 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 419 399 354 285 239
85 41 27
BspEI T'CCGG_A
Cuts at: 5499 5499
Size: 5836
BspHI T'CATG_A
Cuts at: 3725 5455 3725
Size: 1730 4106
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2250 3102 3191 844
Size: 1406 852 89 3489
Fragments arranged by size:
3489 1406 852 89
BsrBI CCG'CTC
Cuts at: 1394 1823 3723 1394
Size: 429 1900 3507
Fragments arranged by size:
3507 1900 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4331 4505 65
Size: 1183 3083 174 1396
Fragments arranged by size:
3083 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2967 4480 4667 1700
Size: 110 1157 1513 187 2869
Fragments arranged by size:
2869 1513 1157 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5836
BssHII G'CGCG_C
Cuts at: 1925 2245 1925
Size: 320 5516
BssSI C'ACGA_G
Cuts at: 3896 4908 3896
Size: 1012 4824
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5836
BstBI TT'CG_AA
Cuts at: 4640 4640
Size: 5836
BstEII G'GTnAC_C
Cuts at: 1184 2608 1184
Size: 1424 4412
BstXI CCAn_nnnn'nTGG
Cuts at: 2458 2845 2458
Size: 387 5449
BstYI r'GATC_y
Cuts at: 1972 3166 3533 3918 3935 5387 5473 5485 5831
Size: 1194 367 385 17 1452 86 12 346
Cuts at: 5831 1972
Size: 1977
Fragments arranged by size:
1977 1452 1194 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2145 2171 2820 2832 513
Size: 552 939 141 26 649 12 3517
Fragments arranged by size:
3517 939 649 552 141 26 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5097
Fragments arranged by size:
5097 683 56
BtsI GCAGTG_nn'
Cuts at: 2717 3352 4146 4166 2717
Size: 635 794 20 4387
Fragments arranged by size:
4387 794 635 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5836
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5801
DraI TTT'AAA
Cuts at: 3140 3140
Size: 5836
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2501 2795 4843 809
Size: 507 1185 294 2048 1802
Fragments arranged by size:
2048 1802 1185 507 294
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2835 2856 3283
Size: 54 1425 50 275 18 1005 21 427
Cuts at: 3283 4172 5801 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1005 889 427 275 54 50
43 21 18
EagI C'GGCC_G
Cuts at: 1537 1812 3283 1537
Size: 275 1471 4090
Fragments arranged by size:
4090 1471 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2547 3766 1353
Size: 1194 1219 3423
Fragments arranged by size:
3423 1219 1194
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2732 4423 4797 4943 387
Size: 1064 138 1143 1691 374 146 1280
Fragments arranged by size:
1691 1280 1143 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2083 2885 3899 4496 5283 5551 1239
Size: 393 451 802 1014 597 787 268 1524
Fragments arranged by size:
1524 1014 802 787 597 451 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2927 2927
Size: 5836
EcoO109I rG'GnC_Cy
Cuts at: 2254 2920 2254
Size: 666 5170
EcoRI G'AATT_C
Cuts at: 3145 3523 3145
Size: 378 5458
FspI TGC'GCA
Cuts at: 2411 4342 2411
Size: 1931 3905
HaeII r_GCGC'y
Cuts at: 1678 2036 2195 2272 2517 4983 1678
Size: 358 159 77 245 2466 2531
Fragments arranged by size:
2531 2466 358 245 159 77
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4477 4509 4551 4583 1373
Size: 32 135 32 2905 32 42 32 2626
Fragments arranged by size:
2905 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3180 3426 669
Size: 988 281 1242 246 3079
Fragments arranged by size:
3079 1242 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5836
HpaI GTT'AAC
Cuts at: 3426 3426
Size: 5836
KpnI G_GTAC'C
Cuts at: 3094 3161 3094
Size: 67 5769
MfeI C'AATT_G
Cuts at: 3435 3435
Size: 5836
MluI A'CGCG_T
Cuts at: 5712 5712
Size: 5836
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4950 5134 1305
Size: 3645 184 2007
Fragments arranged by size:
3645 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2837 10
Size: 54 1425 343 1005 3009
Fragments arranged by size:
3009 1425 1005 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2456 3794 4153 4312 518
Size: 648 1290 1338 359 159 2042
Fragments arranged by size:
2042 1338 1290 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2089 2173 2308 2855 3252 3929 5077 5322
Size: 483 84 135 547 397 677 1148 245
Cuts at: 5322 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 547 483 397 245 135
84
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4345
Fragments arranged by size:
4345 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5836
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5836
NotI GC'GGCC_GC
Cuts at: 3283 3283
Size: 5836
NsiI A_TGCA'T
Cuts at: 3052 3052
Size: 5836
NspI r_CATG'y
Cuts at: 1132 1229 3194 4739 1132
Size: 97 1965 1545 2229
Fragments arranged by size:
2229 1965 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2200 2212 1348
Size: 852 12 4972
Fragments arranged by size:
4972 852 12
PciI A'CATG_T
Cuts at: 4735 4735
Size: 5836
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2392 1108
Size: 1284 4552
PfoI T'CCnGG_A
Cuts at: 2901 2901
Size: 5836
PmeI GTTT'AAAC
Cuts at: 3140 3140
Size: 5836
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2411 2443 2795 2827 3905 3937 5418 5450 2411
Size: 32 352 32 1078 32 1481 32 2797
Fragments arranged by size:
2797 1481 1078 352 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2254 2920 2254
Size: 666 5170
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5836
PsiI TTA'TAA
Cuts at: 3406 3406
Size: 5836
PstI C_TGCA'G
Cuts at: 830 2155 2307 2345 3188 830
Size: 1325 152 38 843 3478
Fragments arranged by size:
3478 1325 843 152 38
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5836
PvuII CAG'CTG
Cuts at: 2308 2308
Size: 5836
SacII CC_GC'GG
Cuts at: 2174 2174
Size: 5836
SalI G'TCGA_C
Cuts at: 1936 3178 1936
Size: 1242 4594
SbfI CC_TGCA'GG
Cuts at: 3188 3188
Size: 5836
ScaI AGT'ACT
Cuts at: 2630 4084 5796 2630
Size: 1454 1712 2670
Fragments arranged by size:
2670 1712 1454
SexAI A'CCwGG_T
Cuts at: 2385 2529 2385
Size: 144 5692
SfcI C'TryA_G
Cuts at: 826 1045 2151 2303 2341 2437 3184 4319 5000
Size: 219 1106 152 38 96 747 1135 681
Cuts at: 5000 5191 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1106 747 681 219 191 152
96 38
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5836
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5836
SmaI CCC'GGG
Cuts at: 1872 3163 1872
Size: 1291 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2519 2882 3940 4841 5103 820
Size: 197 926 576 363 1058 901 262 1553
Fragments arranged by size:
1553 1058 926 901 576 363 262 197
SnaBI TAC'GTA
Cuts at: 493 3627 493
Size: 3134 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5836
SphI G_CATG'C
Cuts at: 1132 3194 1132
Size: 2062 3774
SspI AAT'ATT
Cuts at: 5 52 3760 5
Size: 47 3708 2081
Fragments arranged by size:
3708 2081 47
StuI AGG'CCT
Cuts at: 1517 3122 1517
Size: 1605 4231
StyI C'CwwG_G
Cuts at: 513 1065 2004 2055 3275 513
Size: 552 939 51 1220 3074
Fragments arranged by size:
3074 1220 939 552 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2037 2627 2837 2935 3863 3880 4033
Size: 1255 457 590 210 98 928 17 153
Cuts at: 4033 4218 4663 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 928 590 457 445 210 185
153 98 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2322 2628 2841
Size: 274 80 33 51 1443 345 306 213
Cuts at: 2841 4082 5794 96
Size: 1241 1712 138
Fragments arranged by size:
1712 1443 1241 345 306 274 213 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2687 2719 2726 2758 5796 5828 2687
Size: 32 7 32 3038 32 2695
Fragments arranged by size:
3038 2695 32 32 32 7
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5836
XbaI T'CTAG_A
Cuts at: 3172 3172
Size: 5836
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2666 2773 2666
Size: 107 5729
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5836
XmaI C'CCGG_G
Cuts at: 1870 3161 1870
Size: 1291 4545
XmnI GAAnn'nnTTC
Cuts at: 3965 3965
Size: 5836
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3644 276
Size: 53 83 186 936 2110 2468
Fragments arranged by size:
2468 2110 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PspOMI PspXI PsrI RsrII SacI SanDI SapI
SfiI SfoI SrfI SwaI