Restriction map for
pFN21AB8680
(Circular) (Six-base) MAPSORT of: pFN21AB8680.seq Check: 4909 from: 1 to: 6604
pFN21AB8680 6604 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3813 4414 278
Size: 53 83 186 936 2277 601 2468
Fragments arranged by size:
2468 2277 936 601 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2880 3947 1937
Size: 943 1067 4594
Fragments arranged by size:
4594 1067 943
Acc65I G'GTAC_C
Cuts at: 3925 3925
Size: 6604
AclI AA'CG_TT
Cuts at: 1563 4731 5104 1563
Size: 3168 373 3063
Fragments arranged by size:
3168 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2775 3033 3267 3717 4667 6051 6319 1632
Size: 1143 258 234 450 950 1384 268 1917
Fragments arranged by size:
1917 1384 1143 950 450 268 258 234
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6407
AflIII A'CryG_T
Cuts at: 1203 3078 3583 3901 5503 6480 1203
Size: 1875 505 318 1602 977 1327
Fragments arranged by size:
1875 1602 1327 977 505 318
AgeI A'CCGG_T
Cuts at: 5435 5435
Size: 6604
AhdI GACnn_n'nnGTC
Cuts at: 5333 5333
Size: 6604
AleI CACnn'nnGTG
Cuts at: 1166 2823 1166
Size: 1657 4947
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3242 3467 1184
Size: 2058 225 4321
Fragments arranged by size:
4321 2058 225
AlwNI CAG_nnn'CTG
Cuts at: 2141 4347 5919 2141
Size: 2206 1572 2826
Fragments arranged by size:
2826 2206 1572
ApaLI G'TGCA_C
Cuts at: 2079 4661 5817 2079
Size: 2582 1156 2866
Fragments arranged by size:
2866 2582 1156
ApoI r'AATT_y
Cuts at: 1493 3913 4139 4291 6400 1493
Size: 2420 226 152 2109 1697
Fragments arranged by size:
2420 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5158 160
Size: 872 4126 1606
Fragments arranged by size:
4126 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6604
AvaI C'yCGr_G
Cuts at: 1870 1943 2429 3929 1870
Size: 73 486 1500 4545
Fragments arranged by size:
4545 1500 486 73
BamHI G'GATC_C
Cuts at: 3934 4301 3934
Size: 367 6237
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3864 3925 5380 618
Size: 325 217 627 2077 61 1455 1842
Fragments arranged by size:
2077 1842 1455 627 325 217 61
BanII G_rGCy'C
Cuts at: 1391 2430 2448 3923 1391
Size: 1039 18 1475 4072
Fragments arranged by size:
4072 1475 1039 18
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2361 2877 3420 928
Size: 971 462 516 543 4112
Fragments arranged by size:
4112 971 543 516 462
BbvCI CC'TCA_GC
Cuts at: 2384 2439 2619 2384
Size: 55 180 6369
Fragments arranged by size:
6369 180 55
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4795 4829 1903
Size: 34 50 34 2774 34 3678
Fragments arranged by size:
3678 2774 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4496 5706 4496
Size: 1210 5394
BclI T'GATC_A
Cuts at: 1548 2956 3172 1548
Size: 1408 216 4980
Fragments arranged by size:
4980 1408 216
BfrBI ATG'CAT
Cuts at: 2934 2934
Size: 6604
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5215 136
Size: 107 122 71 631 605 3543 1525
Fragments arranged by size:
3543 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6599 6599
Size: 6604
BlpI GC'TnA_GC
Cuts at: 2657 3215 3440 4021 2657
Size: 558 225 581 5240
Fragments arranged by size:
5240 581 558 225
Bme1580I G_kGCm'C
Cuts at: 1790 2083 4665 5821 1790
Size: 293 2582 1156 2573
Fragments arranged by size:
2582 2573 1156 293
BmgBI CAC'GTC
Cuts at: 1318 3604 3829 1318
Size: 2286 225 4093
Fragments arranged by size:
4093 2286 225
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2488 4335 5293
Size: 416 53 83 399 24 1071 1847 958
Cuts at: 5293 442
Size: 1753
Fragments arranged by size:
1847 1753 1071 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6604
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2537 2804 5264 1239
Size: 1298 267 2460 2579
Fragments arranged by size:
2579 2460 1298 267
Bpu10I CC'TnA_GC
Cuts at: 2384 2439 2447 2619 2384
Size: 55 8 172 6369
Fragments arranged by size:
6369 172 55 8
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2292 4729 5594 5892 2292
Size: 2437 865 298 3004
Fragments arranged by size:
3004 2437 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5267 882
Size: 617 3768 2219
Fragments arranged by size:
3768 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4395 493
Size: 711 3191 2702
Fragments arranged by size:
3191 2702 711
BsaBI GATnn'nnATC
Cuts at: 6598 6598
Size: 6604
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3810 4411 4793 5428
Size: 53 83 186 936 2277 601 382 635
Cuts at: 5428 275
Size: 1451
Fragments arranged by size:
2277 1451 936 635 601 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2198 2569 5037 5435 5709 5856 6267 2198
Size: 371 2468 398 274 147 411 2535
Fragments arranged by size:
2535 2468 411 398 371 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2586 2616 1178
Size: 30 165 30 1183 30 5166
Fragments arranged by size:
5166 1183 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2438 2456 2552 2717 2801 3032 1186
Size: 1252 18 96 165 84 231 4758
Fragments arranged by size:
4758 1252 231 165 96 84 18
BseYI C'CCAG_C
Cuts at: 2250 5807 6303 2250
Size: 3557 496 2551
Fragments arranged by size:
3557 2551 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2765 1163
Size: 573 1029 5002
Fragments arranged by size:
5002 1029 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4054 4815 5843 1540
Size: 275 186 2053 761 1028 2301
Fragments arranged by size:
2301 2053 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2083 2430 2448 3110 4665 4750 5821 2083
Size: 347 18 662 1555 85 1071 2866
Fragments arranged by size:
2866 1555 1071 662 347 85 18
BsmI GAATG_Cn'
Cuts at: 4115 4208 4115
Size: 93 6511
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2083 2430 2448 3110 3923 4665 4750
Size: 399 293 347 18 662 813 742 85
Cuts at: 4750 5821 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 813 742 662 399 347 293
85 18
BspEI T'CCGG_A
Cuts at: 2569 6267 2569
Size: 3698 2906
BspHI T'CATG_A
Cuts at: 2908 2959 4493 6223 2908
Size: 51 1534 1730 3289
Fragments arranged by size:
3289 1730 1534 51
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3959 844
Size: 3115 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2395 2535 4491 1394
Size: 429 572 140 1956 3507
Fragments arranged by size:
3507 1956 572 429 140
BsrDI GCAATG_nn'
Cuts at: 65 1248 3344 5099 5273 65
Size: 1183 2096 1755 174 1396
Fragments arranged by size:
2096 1755 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2186 2331 5248 5435 1700
Size: 110 376 145 2917 187 2869
Fragments arranged by size:
2917 2869 376 187 145 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6604
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6604
BssSI C'ACGA_G
Cuts at: 4664 5676 4664
Size: 1012 5592
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6604
BstBI TT'CG_AA
Cuts at: 5408 5408
Size: 6604
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6604
BstYI r'GATC_y
Cuts at: 1972 2128 3934 4301 4686 4703 6155 6241 6253
Size: 156 1806 367 385 17 1452 86 12
Cuts at: 6253 6599 1972
Size: 346 1977
Fragments arranged by size:
1977 1806 1452 385 367 346 156 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2772 3471 2772
Size: 699 5905
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6052
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5865
Fragments arranged by size:
5865 683 56
BtsI GCAGTG_nn'
Cuts at: 4120 4914 4934 4120
Size: 794 20 5790
Fragments arranged by size:
5790 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6604
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2804 2839 529
Size: 35 2240 35 4294
Fragments arranged by size:
4294 2240 35 35
DraI TTT'AAA
Cuts at: 3908 3908
Size: 6604
DraIII CAC_nnn'GTG
Cuts at: 2102 2102
Size: 6604
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5611 809
Size: 507 4295 1802
Fragments arranged by size:
4295 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2325 4051 4940
Size: 54 1425 50 275 18 495 1726 889
Cuts at: 4940 6569 8
Size: 1629 43
Fragments arranged by size:
1726 1629 1425 889 495 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4051 1537
Size: 275 2239 4090
Fragments arranged by size:
4090 2239 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2158 2403 3009 3399 3684 4534 1353
Size: 805 245 606 390 285 850 3423
Fragments arranged by size:
3423 850 805 606 390 285 245
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5191 5565 5711 387
Size: 1064 138 3602 374 146 1280
Fragments arranged by size:
3602 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2428 2446 2428
Size: 18 6586
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2537 2775 2804 3033 3267 3717 4667
Size: 393 905 238 29 229 234 450 950
Cuts at: 4667 5264 6051 6319 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 950 905 787 597 450 393 268
238 234 229 29
EcoO109I rG'GnC_Cy
Cuts at: 2051 2557 2599 2051
Size: 506 42 6056
Fragments arranged by size:
6056 506 42
EcoRI G'AATT_C
Cuts at: 3913 4291 3913
Size: 378 6226
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2432 2432
Size: 6604
FspI TGC'GCA
Cuts at: 5110 5110
Size: 6604
HaeII r_GCGC'y
Cuts at: 1678 5751 1678
Size: 4073 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2586 2618 2888 2920 3326
Size: 32 135 32 1014 32 270 32 406
Cuts at: 3326 3358 5245 5277 5319 5351 1373
Size: 32 1887 32 42 32 2626
Fragments arranged by size:
2626 1887 1014 406 270 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2881 3948 4194 669
Size: 988 281 943 1067 246 3079
Fragments arranged by size:
3079 1067 988 943 281 246
HindIII A'AGCT_T
Cuts at: 748 2414 748
Size: 1666 4938
HpaI GTT'AAC
Cuts at: 4194 4194
Size: 6604
KpnI G_GTAC'C
Cuts at: 3929 3929
Size: 6604
MfeI C'AATT_G
Cuts at: 4203 4203
Size: 6604
MluI A'CGCG_T
Cuts at: 6480 6480
Size: 6604
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2156 2856 2901 5718 5902 1305
Size: 851 700 45 2817 184 2007
Fragments arranged by size:
2817 2007 851 700 184 45
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2327 10
Size: 54 1425 343 495 4287
Fragments arranged by size:
4287 1425 495 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2823 3168 4562 4921 5080 518
Size: 648 1657 345 1394 359 159 2042
Fragments arranged by size:
2042 1657 1394 648 359 345 159
MspA1I CmG'CkG
Cuts at: 1606 2254 2614 4020 4697 5845 6090 1606
Size: 648 360 1406 677 1148 245 2120
Fragments arranged by size:
2120 1406 1148 677 648 360 245
NaeI GCC'GGC
Cuts at: 2188 2188
Size: 6604
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6052
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6604
NgoMIV G'CCGG_C
Cuts at: 2186 2186
Size: 6604
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6604
NotI GC'GGCC_GC
Cuts at: 4051 4051
Size: 6604
NsiI A_TGCA'T
Cuts at: 2936 2936
Size: 6604
NspI r_CATG'y
Cuts at: 1132 1229 3082 3761 3962 5507 1132
Size: 97 1853 679 201 1545 2229
Fragments arranged by size:
2229 1853 1545 679 201 97
PasI CC'CwG_GG
Cuts at: 1348 2590 2736 1348
Size: 1242 146 5216
Fragments arranged by size:
5216 1242 146
PciI A'CATG_T
Cuts at: 3078 5503 3078
Size: 2425 4179
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6604
PmeI GTTT'AAAC
Cuts at: 3908 3908
Size: 6604
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4673 4705 6186 6218 4673
Size: 32 1481 32 5059
Fragments arranged by size:
5059 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2599 2599
Size: 6604
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6604
PsiI TTA'TAA
Cuts at: 4174 4174
Size: 6604
PstI C_TGCA'G
Cuts at: 830 3006 3956 830
Size: 2176 950 3478
Fragments arranged by size:
3478 2176 950
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6604
PvuII CAG'CTG
Cuts at: 2254 2614 2254
Size: 360 6244
SacI G_AGCT'C
Cuts at: 2430 2448 2430
Size: 18 6586
SalI G'TCGA_C
Cuts at: 1936 2879 3946 1936
Size: 943 1067 4594
Fragments arranged by size:
4594 1067 943
SapI GCTCTTCn'nnn_
Cuts at: 3684 3684
Size: 6604
SbfI CC_TGCA'GG
Cuts at: 3956 3956
Size: 6604
ScaI AGT'ACT
Cuts at: 4852 6564 4852
Size: 1712 4892
SfcI C'TryA_G
Cuts at: 826 1045 3002 3325 3952 5087 5768 5959 826
Size: 219 1957 323 627 1135 681 191 1471
Fragments arranged by size:
1957 1471 1135 681 627 323 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6604
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6604
SmaI CCC'GGG
Cuts at: 1872 3931 1872
Size: 2059 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2307 4708 5609 5871 820
Size: 197 926 364 2401 901 262 1553
Fragments arranged by size:
2401 1553 926 901 364 262 197
SnaBI TAC'GTA
Cuts at: 493 4395 493
Size: 3902 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6604
SphI G_CATG'C
Cuts at: 1132 3962 1132
Size: 2830 3774
SspI AAT'ATT
Cuts at: 5 52 4528 5
Size: 47 4476 2081
Fragments arranged by size:
4476 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6604
StyI C'CwwG_G
Cuts at: 513 1065 2192 3361 3418 4043 513
Size: 552 1127 1169 57 625 3074
Fragments arranged by size:
3074 1169 1127 625 552 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4631 4648 4801 4986 5431 325
Size: 1255 3051 17 153 185 445 1498
Fragments arranged by size:
3051 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2716 3774 4850
Size: 274 80 33 51 1443 739 1058 1076
Cuts at: 4850 6562 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1076 1058 739 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6564 6596 6564
Size: 32 6572
Tth111I GACn'n_nGTC
Cuts at: 1316 2876 1316
Size: 1560 5044
XbaI T'CTAG_A
Cuts at: 2224 3940 2224
Size: 1716 4888
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6604
XmaI C'CCGG_G
Cuts at: 1870 3929 1870
Size: 2059 4545
XmnI GAAnn'nnTTC
Cuts at: 4733 4733
Size: 6604
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3811 4412 276
Size: 53 83 186 936 2277 601 2468
Fragments arranged by size:
2468 2277 936 601 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BplI BsiWI BsmBI BstXI BstZ17I EcoNI EcoRV FseI
FspAI KasI NarI NruI PacI PfoI PmlI PspOMI
PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI
SrfI SwaI XcmI