Restriction map for
pFN21AB8678
(Circular) (Six-base) MAPSORT of: pFN21AB8678.seq Check: 3613 from: 1 to: 5833
pFN21AB8678 5833 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2574 3643 278
Size: 53 83 186 936 1038 1069 2468
Fragments arranged by size:
2468 1069 1038 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3176 1937
Size: 1239 4594
Acc65I G'GTAC_C
Cuts at: 3154 3154
Size: 5833
AclI AA'CG_TT
Cuts at: 1563 3960 4333 1563
Size: 2397 373 3063
Fragments arranged by size:
3063 2397 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2776 3896 5280 5548 1632
Size: 1144 1120 1384 268 1917
Fragments arranged by size:
1917 1384 1144 1120 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5636
AflIII A'CryG_T
Cuts at: 1203 4732 5709 1203
Size: 3529 977 1327
Fragments arranged by size:
3529 1327 977
AgeI A'CCGG_T
Cuts at: 4664 4664
Size: 5833
AhdI GACnn_n'nnGTC
Cuts at: 4562 4562
Size: 5833
AleI CACnn'nnGTG
Cuts at: 1166 2139 2468 1166
Size: 973 329 4531
Fragments arranged by size:
4531 973 329
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5833
AlwNI CAG_nnn'CTG
Cuts at: 3576 5148 3576
Size: 1572 4261
ApaI G_GGCC'C
Cuts at: 2779 2779
Size: 5833
ApaLI G'TGCA_C
Cuts at: 3890 5046 3890
Size: 1156 4677
ApoI r'AATT_y
Cuts at: 1493 3142 3368 3520 5629 1493
Size: 1649 226 152 2109 1697
Fragments arranged by size:
2109 1697 1649 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4387 160
Size: 872 3355 1606
Fragments arranged by size:
3355 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5833
AvaI C'yCGr_G
Cuts at: 1870 1943 3158 1870
Size: 73 1215 4545
Fragments arranged by size:
4545 1215 73
BamHI G'GATC_C
Cuts at: 2054 3163 3530 2054
Size: 1109 367 4357
Fragments arranged by size:
4357 1109 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2341 3104 3154 4609 618
Size: 325 217 627 554 763 50 1455 1842
Fragments arranged by size:
1842 1455 763 627 554 325 217 50
BanII G_rGCy'C
Cuts at: 1391 2158 2779 3038 3152 1391
Size: 767 621 259 114 4072
Fragments arranged by size:
4072 767 621 259 114
BbeI G_GCGC'C
Cuts at: 2345 2345
Size: 5833
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3105 928
Size: 971 1206 3656
Fragments arranged by size:
3656 1206 971
BbvCI CC'TCA_GC
Cuts at: 2190 2190
Size: 5833
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2539 2573 4024 4058 1903
Size: 34 602 34 1451 34 3678
Fragments arranged by size:
3678 1451 602 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3725 4935 3725
Size: 1210 4623
BclI T'GATC_A
Cuts at: 1548 2086 1548
Size: 538 5295
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4444 136
Size: 107 122 71 631 605 2772 1525
Fragments arranged by size:
2772 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5828 5828
Size: 5833
BlpI GC'TnA_GC
Cuts at: 3250 3250
Size: 5833
Bme1580I G_kGCm'C
Cuts at: 1790 2409 2511 2779 3109 3894 5050 1790
Size: 619 102 268 330 785 1156 2573
Fragments arranged by size:
2573 1156 785 619 330 268 102
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5833
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3564 4522 442
Size: 416 53 83 399 24 2147 958 1753
Fragments arranged by size:
2147 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5833
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4493 1239
Size: 3254 2579
Bpu10I CC'TnA_GC
Cuts at: 2190 2190
Size: 5833
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3958 4823 5121 3958
Size: 865 298 4670
Fragments arranged by size:
4670 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2387 3082 4496 882
Size: 617 888 695 1414 2219
Fragments arranged by size:
2219 1414 888 695 617
BsaAI yAC'GTr
Cuts at: 493 1204 3024 3624 493
Size: 711 1820 600 2702
Fragments arranged by size:
2702 1820 711 600
BsaBI GATnn'nnATC
Cuts at: 5827 5827
Size: 5833
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2342 2571 3640 4022
Size: 53 83 186 936 809 229 1069 382
Cuts at: 4022 4657 275
Size: 635 1451
Fragments arranged by size:
1451 1069 936 809 635 382 229 186
83 53
BsaWI w'CCGG_w
Cuts at: 2057 4266 4664 4938 5085 5496 2057
Size: 2209 398 274 147 411 2394
Fragments arranged by size:
2394 2209 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2999 3029 1178
Size: 30 165 30 1596 30 3982
Fragments arranged by size:
3982 1596 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2207 2330 2828 1186
Size: 1021 123 498 4191
Fragments arranged by size:
4191 1021 498 123
BseYI C'CCAG_C
Cuts at: 5036 5532 5036
Size: 496 5337
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2982 1163
Size: 573 1246 4014
Fragments arranged by size:
4014 1246 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3283 4044 5072 1540
Size: 275 186 1282 761 1028 2301
Fragments arranged by size:
2301 1282 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2199 2331 3894 3979 5050 2199
Size: 132 1563 85 1071 2982
Fragments arranged by size:
2982 1563 1071 132 85
BsmI GAATG_Cn'
Cuts at: 3344 3437 3344
Size: 93 5740
BsmBI CGTCTCn'nnnn_
Cuts at: 2578 2693 2578
Size: 115 5718
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2158 2199 2331 2409 2511 2779 3038
Size: 399 368 41 132 78 102 268 259
Cuts at: 3038 3109 3152 3894 3979 5050 1391
Size: 71 43 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 368 268 259 132
102 85 78 71 43 41
BspEI T'CCGG_A
Cuts at: 5496 5496
Size: 5833
BspHI T'CATG_A
Cuts at: 3722 5452 3722
Size: 1730 4103
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2604 2739 2880 3188 844
Size: 1760 135 141 308 3489
Fragments arranged by size:
3489 1760 308 141 135
BsrBI CCG'CTC
Cuts at: 1394 1823 2411 3720 1394
Size: 429 588 1309 3507
Fragments arranged by size:
3507 1309 588 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4328 4502 65
Size: 1183 3080 174 1396
Fragments arranged by size:
3080 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4477 4664 1700
Size: 110 2667 187 2869
Fragments arranged by size:
2869 2667 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5833
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5833
BssSI C'ACGA_G
Cuts at: 2831 3893 4905 2831
Size: 1062 1012 3759
Fragments arranged by size:
3759 1062 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2326 794
Size: 1532 4301
BstBI TT'CG_AA
Cuts at: 4637 4637
Size: 5833
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5833
BstXI CCAn_nnnn'nTGG
Cuts at: 2470 2470
Size: 5833
BstYI r'GATC_y
Cuts at: 1972 2054 2091 3163 3530 3915 3932 5384 5470
Size: 82 37 1072 367 385 17 1452 86
Cuts at: 5470 5482 5828 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1072 385 367 346 86 82
37 17 12
Bsu36I CC'TnA_GG
Cuts at: 3003 3003
Size: 5833
BtgI C'CryG_G
Cuts at: 513 1065 2004 2226 513
Size: 552 939 222 4120
Fragments arranged by size:
4120 939 552 222
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2542 487
Size: 683 56 1316 3778
Fragments arranged by size:
3778 1316 683 56
BtsI GCAGTG_nn'
Cuts at: 3349 4143 4163 3349
Size: 794 20 5019
Fragments arranged by size:
5019 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5833
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2100 2135 3010 3045 529
Size: 35 1536 35 875 35 3317
Fragments arranged by size:
3317 1536 875 35 35 35
DraI TTT'AAA
Cuts at: 2349 3137 2349
Size: 788 5045
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4840 809
Size: 507 3524 1802
Fragments arranged by size:
3524 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3280 4169 5798
Size: 54 1425 50 275 18 1450 889 1629
Cuts at: 5798 8
Size: 43
Fragments arranged by size:
1629 1450 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3280 1537
Size: 275 1468 4090
Fragments arranged by size:
4090 1468 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2795 3763 1353
Size: 1442 968 3423
Fragments arranged by size:
3423 1442 968
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4420 4794 4940 387
Size: 1064 138 2831 374 146 1280
Fragments arranged by size:
2831 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2776 3896 4493 5280 5548 1239
Size: 393 1144 1120 597 787 268 1524
Fragments arranged by size:
1524 1144 1120 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2336 2600 2336
Size: 264 5569
EcoO109I rG'GnC_Cy
Cuts at: 2776 2836 2776
Size: 60 5773
EcoRI G'AATT_C
Cuts at: 3142 3520 3142
Size: 378 5455
EcoRV GAT'ATC
Cuts at: 2994 2994
Size: 5833
FspI TGC'GCA
Cuts at: 2549 4339 2549
Size: 1790 4043
FspAI rTGC'GCAy
Cuts at: 2549 2549
Size: 5833
HaeII r_GCGC'y
Cuts at: 1678 2345 4980 1678
Size: 667 2635 2531
Fragments arranged by size:
2635 2531 667
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2039 2071 2606 2638 4474
Size: 32 135 32 467 32 535 32 1836
Cuts at: 4474 4506 4548 4580 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1836 535 467 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3177 3423 669
Size: 988 281 1239 246 3079
Fragments arranged by size:
3079 1239 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5833
HpaI GTT'AAC
Cuts at: 3423 3423
Size: 5833
KasI G'GCGC_C
Cuts at: 2341 2341
Size: 5833
KpnI G_GTAC'C
Cuts at: 3158 3158
Size: 5833
MfeI C'AATT_G
Cuts at: 3432 3432
Size: 5833
MluI A'CGCG_T
Cuts at: 5709 5709
Size: 5833
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4947 5131 1305
Size: 3642 184 2007
Fragments arranged by size:
3642 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4011
Fragments arranged by size:
4011 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2139 2423 2468 3791 4150 4309 518
Size: 648 973 284 45 1323 359 159 2042
Fragments arranged by size:
2042 1323 973 648 359 284 159 45
MspA1I CmG'CkG
Cuts at: 1606 2299 3249 3926 5074 5319 1606
Size: 693 950 677 1148 245 2120
Fragments arranged by size:
2120 1148 950 693 677 245
NarI GG'CG_CC
Cuts at: 2342 2342
Size: 5833
NcoI C'CATG_G
Cuts at: 513 1065 2004 2226 513
Size: 552 939 222 4120
Fragments arranged by size:
4120 939 552 222
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5833
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5833
NotI GC'GGCC_GC
Cuts at: 3280 3280
Size: 5833
NspI r_CATG'y
Cuts at: 1132 1229 3191 4736 1132
Size: 97 1962 1545 2229
Fragments arranged by size:
2229 1962 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5833
PciI A'CATG_T
Cuts at: 4732 4732
Size: 5833
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5833
PmeI GTTT'AAAC
Cuts at: 3137 3137
Size: 5833
PmlI CAC'GTG
Cuts at: 3024 3024
Size: 5833
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3902 3934 5415 5447 3902
Size: 32 1481 32 4288
Fragments arranged by size:
4288 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2836 2836
Size: 5833
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5833
PsiI TTA'TAA
Cuts at: 3403 3403
Size: 5833
PspOMI G'GGCC_C
Cuts at: 2775 2775
Size: 5833
PstI C_TGCA'G
Cuts at: 830 2304 3185 830
Size: 1474 881 3478
Fragments arranged by size:
3478 1474 881
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5833
PvuII CAG'CTG
Cuts at: 2299 2299
Size: 5833
SalI G'TCGA_C
Cuts at: 1936 3175 1936
Size: 1239 4594
SapI GCTCTTCn'nnn_
Cuts at: 2795 2795
Size: 5833
SbfI CC_TGCA'GG
Cuts at: 3185 3185
Size: 5833
ScaI AGT'ACT
Cuts at: 2726 4081 5793 2726
Size: 1355 1712 2766
Fragments arranged by size:
2766 1712 1355
SfcI C'TryA_G
Cuts at: 826 1045 2300 2451 3181 4316 4997 5188 826
Size: 219 1255 151 730 1135 681 191 1471
Fragments arranged by size:
1471 1255 1135 730 681 219 191 151
SfoI GGC'GCC
Cuts at: 2343 2343
Size: 5833
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5833
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5833
SmaI CCC'GGG
Cuts at: 1872 3160 1872
Size: 1288 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3937 4838 5100 820
Size: 197 926 1994 901 262 1553
Fragments arranged by size:
1994 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3624 493
Size: 3131 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5833
SphI G_CATG'C
Cuts at: 1132 3191 1132
Size: 2059 3774
SspI AAT'ATT
Cuts at: 5 52 3757 5
Size: 47 3705 2081
Fragments arranged by size:
3705 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5833
StyI C'CwwG_G
Cuts at: 513 1065 2004 2226 3272 513
Size: 552 939 222 1046 3074
Fragments arranged by size:
3074 1046 939 552 222
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2513 3860 3877 4030 4215 4660 325
Size: 1255 933 1347 17 153 185 445 1498
Fragments arranged by size:
1498 1347 1255 933 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2434 2724 4079
Size: 274 80 33 51 1443 457 290 1355
Cuts at: 4079 5791 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1355 457 290 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2046 2078 5793 5825 2046
Size: 32 3715 32 2054
Fragments arranged by size:
3715 2054 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5833
XbaI T'CTAG_A
Cuts at: 3169 3169
Size: 5833
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5833
XmaI C'CCGG_G
Cuts at: 1870 3158 1870
Size: 1288 4545
XmnI GAAnn'nnTTC
Cuts at: 3962 3962
Size: 5833
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2572 3641 276
Size: 53 83 186 936 1038 1069 2468
Fragments arranged by size:
2468 1069 1038 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FspI FspAI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BstZ17I DraIII EcoICRI FalI FseI NaeI NgoMIV
NruI NsiI PacI PfoI PspXI PsrI RsrII SacI
SacII SanDI SexAI SfiI SrfI SwaI XcmI