Restriction map for
pFN21AB8677
(Circular) (Six-base) MAPSORT of: pFN21AB8677.seq Check: 5305 from: 1 to: 5809
pFN21AB8677 5809 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3619 278
Size: 53 83 186 936 2083 2468
Fragments arranged by size:
2468 2083 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2132 3152 1937
Size: 195 1020 4594
Fragments arranged by size:
4594 1020 195
Acc65I G'GTAC_C
Cuts at: 3130 3130
Size: 5809
AclI AA'CG_TT
Cuts at: 1563 3936 4309 1563
Size: 2373 373 3063
Fragments arranged by size:
3063 2373 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3872 5256 5524 1632
Size: 2240 1384 268 1917
Fragments arranged by size:
2240 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5612
AflIII A'CryG_T
Cuts at: 1203 2728 2781 2991 4708 5685 1203
Size: 1525 53 210 1717 977 1327
Fragments arranged by size:
1717 1525 1327 977 210 53
AgeI A'CCGG_T
Cuts at: 4640 4640
Size: 5809
AhdI GACnn_n'nnGTC
Cuts at: 4538 4538
Size: 5809
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5809
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5809
AlwNI CAG_nnn'CTG
Cuts at: 2049 2413 2465 3552 5124 2049
Size: 364 52 1087 1572 2734
Fragments arranged by size:
2734 1572 1087 364 52
ApaI G_GGCC'C
Cuts at: 2032 2077 2503 2032
Size: 45 426 5338
Fragments arranged by size:
5338 426 45
ApaLI G'TGCA_C
Cuts at: 3866 5022 3866
Size: 1156 4653
ApoI r'AATT_y
Cuts at: 1493 2773 3118 3344 3496 5605 1493
Size: 1280 345 226 152 2109 1697
Fragments arranged by size:
2109 1697 1280 345 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4363 160
Size: 872 3331 1606
Fragments arranged by size:
3331 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5809
AvaI C'yCGr_G
Cuts at: 1870 1943 2643 3134 1870
Size: 73 700 491 4545
Fragments arranged by size:
4545 700 491 73
BamHI G'GATC_C
Cuts at: 3139 3506 3139
Size: 367 5442
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2151 2212 2876 3130 4585
Size: 325 217 627 364 61 664 254 1455
Cuts at: 4585 618
Size: 1842
Fragments arranged by size:
1842 1455 664 627 364 325 254 217
61
BanII G_rGCy'C
Cuts at: 1391 2032 2077 2503 2694 3128 1391
Size: 641 45 426 191 434 4072
Fragments arranged by size:
4072 641 434 426 191 45
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2547 3089 928
Size: 971 648 542 3648
Fragments arranged by size:
3648 971 648 542
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4000 4034 1903
Size: 34 2063 34 3678
Fragments arranged by size:
3678 2063 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2813 3701 4911 2813
Size: 888 1210 3711
Fragments arranged by size:
3711 1210 888
BclI T'GATC_A
Cuts at: 1548 2566 1548
Size: 1018 4791
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2027 4420 136
Size: 107 122 71 631 605 355 2393 1525
Fragments arranged by size:
2393 1525 631 605 355 122 107 71
BglII A'GATC_T
Cuts at: 2450 5804 2450
Size: 3354 2455
BlpI GC'TnA_GC
Cuts at: 3226 3226
Size: 5809
Bme1580I G_kGCm'C
Cuts at: 1790 2032 2077 2503 2991 3002 3870 5026 1790
Size: 242 45 426 488 11 868 1156 2573
Fragments arranged by size:
2573 1156 868 488 426 242 45 11
BmgBI CAC'GTC
Cuts at: 1318 2965 1318
Size: 1647 4162
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2079 3115 3540
Size: 416 53 83 399 24 662 1036 425
Cuts at: 3540 4498 442
Size: 958 1753
Fragments arranged by size:
1753 1036 958 662 425 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5809
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2133 2322 2708 2875 4469 1239
Size: 894 189 386 167 1594 2579
Fragments arranged by size:
2579 1594 894 386 189 167
Bpu10I CC'TnA_GC
Cuts at: 2654 2654
Size: 5809
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3934 4799 5097 3934
Size: 865 298 4646
Fragments arranged by size:
4646 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2231 4472 882
Size: 617 732 2241 2219
Fragments arranged by size:
2241 2219 732 617
BsaAI yAC'GTr
Cuts at: 493 1204 2729 2782 3600 493
Size: 711 1525 53 818 2702
Fragments arranged by size:
2702 1525 818 711 53
BsaBI GATnn'nnATC
Cuts at: 2703 5803 2703
Size: 3100 2709
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3616 3998 4633 275
Size: 53 83 186 936 2083 382 635 1451
Fragments arranged by size:
2083 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4242 4640 4914 5061 5472 4242
Size: 398 274 147 411 4579
Fragments arranged by size:
4579 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5584
Fragments arranged by size:
5584 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5809
BseYI C'CCAG_C
Cuts at: 5012 5508 5012
Size: 496 5313
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5236
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2063 2106 3259 4020 5048 1540
Size: 275 186 62 43 1153 761 1028 2301
Fragments arranged by size:
2301 1153 1028 761 275 186 62 43
BsiHKAI G_wGCw'C
Cuts at: 2694 3870 3955 5026 2694
Size: 1176 85 1071 3477
Fragments arranged by size:
3477 1176 1071 85
BsmI GAATG_Cn'
Cuts at: 3320 3413 3320
Size: 93 5716
BsmBI CGTCTCn'nnnn_
Cuts at: 2642 2971 2642
Size: 329 5480
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2032 2077 2503 2694 2991 3002 3128
Size: 399 242 45 426 191 297 11 126
Cuts at: 3128 3870 3955 5026 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 426 399 297 242 191
126 85 45 11
BspEI T'CCGG_A
Cuts at: 5472 5472
Size: 5809
BspHI T'CATG_A
Cuts at: 3698 5428 3698
Size: 1730 4079
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3164 844
Size: 2320 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2092 2848 3696 1394
Size: 429 269 756 848 3507
Fragments arranged by size:
3507 848 756 429 269
BsrDI GCAATG_nn'
Cuts at: 65 1248 4304 4478 65
Size: 1183 3056 174 1396
Fragments arranged by size:
3056 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4453 4640 1700
Size: 110 2643 187 2869
Fragments arranged by size:
2869 2643 187 110
BsrGI T'GTAC_A
Cuts at: 96 2015 96
Size: 1919 3890
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5809
BssSI C'ACGA_G
Cuts at: 2424 3869 4881 2424
Size: 1445 1012 3352
Fragments arranged by size:
3352 1445 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5809
BstBI TT'CG_AA
Cuts at: 4613 4613
Size: 5809
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5809
BstYI r'GATC_y
Cuts at: 1972 2450 3139 3506 3891 3908 5360 5446 5458
Size: 478 689 367 385 17 1452 86 12
Cuts at: 5458 5804 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 689 478 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3094 3094
Size: 5809
BtgI C'CryG_G
Cuts at: 513 1065 2004 2037 2365 513
Size: 552 939 33 328 3957
Fragments arranged by size:
3957 939 552 328 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5070
Fragments arranged by size:
5070 683 56
BtsI GCAGTG_nn'
Cuts at: 3325 4119 4139 3325
Size: 794 20 4995
Fragments arranged by size:
4995 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5809
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5774
DraI TTT'AAA
Cuts at: 3113 3113
Size: 5809
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4816 809
Size: 507 3500 1802
Fragments arranged by size:
3500 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2034 2081 2103
Size: 54 1425 50 275 18 204 47 22
Cuts at: 2103 2362 3256 4145 5774 8
Size: 259 894 889 1629 43
Fragments arranged by size:
1629 1425 894 889 275 259 204 54
50 47 43 22 18
EagI C'GGCC_G
Cuts at: 1537 1812 2103 3256 1537
Size: 275 291 1153 4090
Fragments arranged by size:
4090 1153 291 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3739 1353
Size: 2386 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4396 4770 4916 387
Size: 1064 138 2807 374 146 1280
Fragments arranged by size:
2807 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2692 2692
Size: 5809
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2133 2322 2708 2875 3872 4469 5256
Size: 393 501 189 386 167 997 597 787
Cuts at: 5256 5524 1239
Size: 268 1524
Fragments arranged by size:
1524 997 787 597 501 393 386 268
189 167
EcoO109I rG'GnC_Cy
Cuts at: 2029 2073 2500 2658 2029
Size: 44 427 158 5180
Fragments arranged by size:
5180 427 158 44
EcoRI G'AATT_C
Cuts at: 2773 3118 3496 2773
Size: 345 378 5086
Fragments arranged by size:
5086 378 345
FspI TGC'GCA
Cuts at: 4315 4315
Size: 5809
HaeII r_GCGC'y
Cuts at: 1678 2797 4956 1678
Size: 1119 2159 2531
Fragments arranged by size:
2531 2159 1119
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2268 2300 2407 2439 2851
Size: 32 135 32 696 32 107 32 412
Cuts at: 2851 2883 4450 4482 4524 4556 1373
Size: 32 1567 32 42 32 2626
Fragments arranged by size:
2626 1567 696 412 135 107 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2133 3153 3399 669
Size: 988 281 195 1020 246 3079
Fragments arranged by size:
3079 1020 988 281 246 195
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5809
HpaI GTT'AAC
Cuts at: 3399 3399
Size: 5809
KpnI G_GTAC'C
Cuts at: 3134 3134
Size: 5809
MfeI C'AATT_G
Cuts at: 3408 3408
Size: 5809
MluI A'CGCG_T
Cuts at: 5685 5685
Size: 5809
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2169 2605 4923 5107 1305
Size: 864 436 2318 184 2007
Fragments arranged by size:
2318 2007 864 436 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2083 2364 10
Size: 54 1425 343 251 281 3455
Fragments arranged by size:
3455 1425 343 281 251 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2756 3036 3767 4126 4285 518
Size: 648 1590 280 731 359 159 2042
Fragments arranged by size:
2042 1590 731 648 359 280 159
MspA1I CmG'CkG
Cuts at: 1606 2049 3225 3902 5050 5295 1606
Size: 443 1176 677 1148 245 2120
Fragments arranged by size:
2120 1176 1148 677 443 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2365 513
Size: 552 939 361 3957
Fragments arranged by size:
3957 939 552 361
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5809
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5809
NotI GC'GGCC_GC
Cuts at: 3256 3256
Size: 5809
NspI r_CATG'y
Cuts at: 1132 1229 2995 3167 4712 1132
Size: 97 1766 172 1545 2229
Fragments arranged by size:
2229 1766 1545 172 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5809
PciI A'CATG_T
Cuts at: 2991 4708 2991
Size: 1717 4092
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5809
PfoI T'CCnGG_A
Cuts at: 2841 2841
Size: 5809
PmeI GTTT'AAAC
Cuts at: 3113 3113
Size: 5809
PmlI CAC'GTG
Cuts at: 2782 2782
Size: 5809
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3878 3910 5391 5423 3878
Size: 32 1481 32 4264
Fragments arranged by size:
4264 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5809
PsiI TTA'TAA
Cuts at: 3379 3379
Size: 5809
PspOMI G'GGCC_C
Cuts at: 2028 2073 2499 2028
Size: 45 426 5338
Fragments arranged by size:
5338 426 45
PstI C_TGCA'G
Cuts at: 830 2335 2976 3161 830
Size: 1505 641 185 3478
Fragments arranged by size:
3478 1505 641 185
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5809
SacI G_AGCT'C
Cuts at: 2694 2694
Size: 5809
SalI G'TCGA_C
Cuts at: 1936 2131 3151 1936
Size: 195 1020 4594
Fragments arranged by size:
4594 1020 195
SbfI CC_TGCA'GG
Cuts at: 3161 3161
Size: 5809
ScaI AGT'ACT
Cuts at: 4057 5769 4057
Size: 1712 4097
SfcI C'TryA_G
Cuts at: 826 1045 2331 2972 3157 4292 4973 5164 826
Size: 219 1286 641 185 1135 681 191 1471
Fragments arranged by size:
1471 1286 1135 681 641 219 191 185
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5809
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5809
SmaI CCC'GGG
Cuts at: 1872 3136 1872
Size: 1264 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3913 4814 5076 820
Size: 197 926 1970 901 262 1553
Fragments arranged by size:
1970 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3600 493
Size: 3107 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5809
SphI G_CATG'C
Cuts at: 1132 3167 1132
Size: 2035 3774
SspI AAT'ATT
Cuts at: 5 52 3733 5
Size: 47 3681 2081
Fragments arranged by size:
3681 2081 47
StuI AGG'CCT
Cuts at: 1517 3099 1517
Size: 1582 4227
StyI C'CwwG_G
Cuts at: 513 1065 2004 2084 2365 2373 2880 2931 3248
Size: 552 939 80 281 8 507 51 317
Cuts at: 3248 513
Size: 3074
Fragments arranged by size:
3074 939 552 507 317 281 80 51
8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3836 3853 4006 4191 4636 325
Size: 1255 2256 17 153 185 445 1498
Fragments arranged by size:
2256 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2015 2784 4055
Size: 274 80 33 51 1443 38 769 1271
Cuts at: 4055 5767 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1271 769 274 138 80 51
38 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2144 2176 5769 5801 2144
Size: 32 3593 32 2152
Fragments arranged by size:
3593 2152 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2528 1316
Size: 1212 4597
XbaI T'CTAG_A
Cuts at: 3145 3145
Size: 5809
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2518 2518
Size: 5809
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5809
XmaI C'CCGG_G
Cuts at: 1870 3134 1870
Size: 1264 4545
XmnI GAAnn'nnTTC
Cuts at: 2601 3938 2601
Size: 1337 4472
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3617 276
Size: 53 83 186 936 2083 2468
Fragments arranged by size:
2468 2083 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PpuMI PspXI PsrI PvuII RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI