Restriction map for
pFN21AB8674
(Circular) (Six-base) MAPSORT of: pFN21AB8674.seq Check: 1171 from: 1 to: 6280
pFN21AB8674 6280 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3166 3166
Size: 6280
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4090 278
Size: 53 83 186 936 2554 2468
Fragments arranged by size:
2554 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3623 1937
Size: 1686 4594
Acc65I G'GTAC_C
Cuts at: 3601 3601
Size: 6280
AclI AA'CG_TT
Cuts at: 1563 4407 4780 1563
Size: 2844 373 3063
Fragments arranged by size:
3063 2844 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2571 2900 4343 5727 5995 1632
Size: 939 329 1443 1384 268 1917
Fragments arranged by size:
1917 1443 1384 939 329 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6083
AflIII A'CryG_T
Cuts at: 1203 2182 5179 6156 1203
Size: 979 2997 977 1327
Fragments arranged by size:
2997 1327 979 977
AgeI A'CCGG_T
Cuts at: 5111 5111
Size: 6280
AhdI GACnn_n'nnGTC
Cuts at: 5009 5009
Size: 6280
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6280
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6280
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3470 3502 3470
Size: 32 6248
AlwNI CAG_nnn'CTG
Cuts at: 2465 2864 3419 4023 5595 2465
Size: 399 555 604 1572 3150
Fragments arranged by size:
3150 1572 604 555 399
ApaLI G'TGCA_C
Cuts at: 4337 5493 4337
Size: 1156 5124
ApoI r'AATT_y
Cuts at: 1493 2360 2592 3310 3589 3815 3967 6076 1493
Size: 867 232 718 279 226 152 2109 1697
Fragments arranged by size:
2109 1697 867 718 279 232 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4834 160
Size: 872 3802 1606
Fragments arranged by size:
3802 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6280
AvaI C'yCGr_G
Cuts at: 1870 1943 3605 1870
Size: 73 1662 4545
Fragments arranged by size:
4545 1662 73
AvrII C'CTAG_G
Cuts at: 3262 3262
Size: 6280
BamHI G'GATC_C
Cuts at: 3610 3977 3610
Size: 367 5913
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3601 5056 618
Size: 325 217 627 1814 1455 1842
Fragments arranged by size:
1842 1814 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2905 3599 1391
Size: 1514 694 4072
Fragments arranged by size:
4072 1514 694
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2514 928
Size: 971 615 4694
Fragments arranged by size:
4694 971 615
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4471 4505 1903
Size: 34 50 34 2450 34 3678
Fragments arranged by size:
3678 2450 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4172 5382 4172
Size: 1210 5070
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6280
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4891 136
Size: 107 122 71 631 605 3219 1525
Fragments arranged by size:
3219 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3290 6275 3290
Size: 2985 3295
BlpI GC'TnA_GC
Cuts at: 3697 3697
Size: 6280
Bme1580I G_kGCm'C
Cuts at: 1790 4341 5497 1790
Size: 2551 1156 2573
Fragments arranged by size:
2573 2551 1156
BmgBI CAC'GTC
Cuts at: 1318 2041 1318
Size: 723 5557
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4011 4969 442
Size: 416 53 83 399 24 2594 958 1753
Fragments arranged by size:
2594 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6280
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2421 3126 4940 1239
Size: 1182 705 1814 2579
Fragments arranged by size:
2579 1814 1182 705
Bpu10I CC'TnA_GC
Cuts at: 2865 2865
Size: 6280
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4405 5270 5568 4405
Size: 865 298 5117
Fragments arranged by size:
5117 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4943 882
Size: 617 3444 2219
Fragments arranged by size:
3444 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2285 4071 493
Size: 711 1081 1786 2702
Fragments arranged by size:
2702 1786 1081 711
BsaBI GATnn'nnATC
Cuts at: 6274 6274
Size: 6280
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4087 4469 5104 275
Size: 53 83 186 936 2554 382 635 1451
Fragments arranged by size:
2554 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3514 4713 5111 5385 5532 5943 3514
Size: 1199 398 274 147 411 3851
Fragments arranged by size:
3851 1199 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2419 2449 1178
Size: 30 165 30 1016 30 5009
Fragments arranged by size:
5009 1016 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6280
BseYI C'CCAG_C
Cuts at: 5483 5979 5483
Size: 496 5784
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5707
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3730 4491 5519 1540
Size: 275 186 1729 761 1028 2301
Fragments arranged by size:
2301 1729 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4341 4426 5497 4341
Size: 85 1071 5124
Fragments arranged by size:
5124 1071 85
BsmI GAATG_Cn'
Cuts at: 3034 3228 3791 3884 3034
Size: 194 563 93 5430
Fragments arranged by size:
5430 563 194 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2905 3599 4341 4426 5497 1391
Size: 399 1115 694 742 85 1071 2174
Fragments arranged by size:
2174 1115 1071 742 694 399 85
BspEI T'CCGG_A
Cuts at: 5943 5943
Size: 6280
BspHI T'CATG_A
Cuts at: 4169 5899 4169
Size: 1730 4550
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2432 2899 3166 3635 844
Size: 1588 467 267 469 3489
Fragments arranged by size:
3489 1588 469 467 267
BsrBI CCG'CTC
Cuts at: 1394 1823 4167 1394
Size: 429 2344 3507
Fragments arranged by size:
3507 2344 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4775 4949 65
Size: 1183 3527 174 1396
Fragments arranged by size:
3527 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4924 5111 1700
Size: 110 3114 187 2869
Fragments arranged by size:
3114 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6280
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6280
BssSI C'ACGA_G
Cuts at: 4340 5352 4340
Size: 1012 5268
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3332 794
Size: 2538 3742
BstBI TT'CG_AA
Cuts at: 5084 5084
Size: 6280
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6280
BstYI r'GATC_y
Cuts at: 1972 2926 3290 3610 3977 4362 4379 5831 5917
Size: 954 364 320 367 385 17 1452 86
Cuts at: 5917 5929 6275 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 954 385 367 364 346 320
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5728
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5541
Fragments arranged by size:
5541 683 56
BtsI GCAGTG_nn'
Cuts at: 3796 4590 4610 3796
Size: 794 20 5466
Fragments arranged by size:
5466 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6280
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6245
DraI TTT'AAA
Cuts at: 2674 2784 3584 2674
Size: 110 800 5370
Fragments arranged by size:
5370 800 110
DraIII CAC_nnn'GTG
Cuts at: 3206 3206
Size: 6280
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5287 809
Size: 507 3971 1802
Fragments arranged by size:
3971 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3727 4616 6245
Size: 54 1425 50 275 18 1897 889 1629
Cuts at: 6245 8
Size: 43
Fragments arranged by size:
1897 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3727 1537
Size: 275 1915 4090
Fragments arranged by size:
4090 1915 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2211 4210 1353
Size: 858 1999 3423
Fragments arranged by size:
3423 1999 858
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4867 5241 5387 387
Size: 1064 138 3278 374 146 1280
Fragments arranged by size:
3278 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2421 2571 2900 3126 4343 4940 5727
Size: 393 789 150 329 226 1217 597 787
Cuts at: 5727 5995 1239
Size: 268 1524
Fragments arranged by size:
1524 1217 789 787 597 393 329 268
226 150
EcoNI CCTnn'n_nnAGG
Cuts at: 2439 2439
Size: 6280
EcoO109I rG'GnC_Cy
Cuts at: 2371 3266 2371
Size: 895 5385
EcoRI G'AATT_C
Cuts at: 3310 3589 3967 3310
Size: 279 378 5623
Fragments arranged by size:
5623 378 279
EcoRV GAT'ATC
Cuts at: 2391 2977 2391
Size: 586 5694
FspI TGC'GCA
Cuts at: 4786 4786
Size: 6280
HaeII r_GCGC'y
Cuts at: 1678 5427 1678
Size: 3749 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2911 2943 3452 3484 4921
Size: 32 135 32 1339 32 509 32 1437
Cuts at: 4921 4953 4995 5027 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1437 1339 509 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3624 3870 669
Size: 988 281 1686 246 3079
Fragments arranged by size:
3079 1686 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6280
HpaI GTT'AAC
Cuts at: 3870 3870
Size: 6280
KpnI G_GTAC'C
Cuts at: 3605 3605
Size: 6280
MfeI C'AATT_G
Cuts at: 2716 3879 2716
Size: 1163 5117
MluI A'CGCG_T
Cuts at: 6156 6156
Size: 6280
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2092 2954 5394 5578 1305
Size: 787 862 2440 184 2007
Fragments arranged by size:
2440 2007 862 787 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4458
Fragments arranged by size:
4458 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4238 4597 4756 518
Size: 648 3072 359 159 2042
Fragments arranged by size:
3072 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2462 3696 4373 5521 5766 1606
Size: 856 1234 677 1148 245 2120
Fragments arranged by size:
2120 1234 1148 856 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5728
NdeI CA'TA_TG
Cuts at: 387 2099 387
Size: 1712 4568
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6280
NotI GC'GGCC_GC
Cuts at: 3727 3727
Size: 6280
NspI r_CATG'y
Cuts at: 1132 1229 2186 3638 5183 1132
Size: 97 957 1452 1545 2229
Fragments arranged by size:
2229 1545 1452 957 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6280
PciI A'CATG_T
Cuts at: 2182 5179 2182
Size: 2997 3283
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2721 2989 1108
Size: 1613 268 4399
Fragments arranged by size:
4399 1613 268
PfoI T'CCnGG_A
Cuts at: 3066 3066
Size: 6280
PmeI GTTT'AAAC
Cuts at: 3584 3584
Size: 6280
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3383 3415 4349 4381 5862 5894 3383
Size: 32 934 32 1481 32 3769
Fragments arranged by size:
3769 1481 934 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2371 3266 2371
Size: 895 5385
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6280
PsiI TTA'TAA
Cuts at: 3850 3850
Size: 6280
PstI C_TGCA'G
Cuts at: 830 2910 3632 830
Size: 2080 722 3478
Fragments arranged by size:
3478 2080 722
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6280
PvuII CAG'CTG
Cuts at: 2462 2462
Size: 6280
SalI G'TCGA_C
Cuts at: 1936 3622 1936
Size: 1686 4594
SbfI CC_TGCA'GG
Cuts at: 2910 3632 2910
Size: 722 5558
ScaI AGT'ACT
Cuts at: 2119 4528 6240 2119
Size: 2409 1712 2159
Fragments arranged by size:
2409 2159 1712
SfcI C'TryA_G
Cuts at: 826 1045 2070 2906 3256 3407 3628 4763 5444
Size: 219 1025 836 350 151 221 1135 681
Cuts at: 5444 5635 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1025 836 681 350 221 219
191 151
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6280
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6280
SmaI CCC'GGG
Cuts at: 1872 3607 1872
Size: 1735 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4384 5285 5547 820
Size: 197 926 2441 901 262 1553
Fragments arranged by size:
2441 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4071 493
Size: 3578 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6280
SphI G_CATG'C
Cuts at: 1132 3638 1132
Size: 2506 3774
SspI AAT'ATT
Cuts at: 5 52 4204 5
Size: 47 4152 2081
Fragments arranged by size:
4152 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6280
StyI C'CwwG_G
Cuts at: 513 1065 3262 3719 513
Size: 552 2197 457 3074
Fragments arranged by size:
3074 2197 552 457
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4307 4324 4477 4662 5107 325
Size: 1255 2727 17 153 185 445 1498
Fragments arranged by size:
2727 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2061 2117 3114
Size: 274 80 33 51 1443 84 56 997
Cuts at: 3114 4526 6238 96
Size: 1412 1712 138
Fragments arranged by size:
1712 1443 1412 997 274 138 84 80
56 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6240 6272 6240
Size: 32 6248
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6280
XbaI T'CTAG_A
Cuts at: 3616 3616
Size: 6280
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6280
XmaI C'CCGG_G
Cuts at: 1870 3605 1870
Size: 1735 4545
XmnI GAAnn'nnTTC
Cuts at: 2361 4409 2361
Size: 2048 4232
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4088 276
Size: 53 83 186 936 2554 2468
Fragments arranged by size:
2554 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI BaeI BbeI BbvCI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI