Restriction map for
pFN21AB8672
(Circular) (Six-base) MAPSORT of: pFN21AB8672.seq Check: 9407 from: 1 to: 6418
pFN21AB8672 6418 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4228 278
Size: 53 83 186 936 2692 2468
Fragments arranged by size:
2692 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2193 3761 1937
Size: 256 1568 4594
Fragments arranged by size:
4594 1568 256
Acc65I G'GTAC_C
Cuts at: 3739 3739
Size: 6418
AclI AA'CG_TT
Cuts at: 1563 4545 4918 1563
Size: 2982 373 3063
Fragments arranged by size:
3063 2982 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3576 4481 5865 6133 1632
Size: 1944 905 1384 268 1917
Fragments arranged by size:
1944 1917 1384 905 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6221
AflIII A'CryG_T
Cuts at: 1203 3297 5317 6294 1203
Size: 2094 2020 977 1327
Fragments arranged by size:
2094 2020 1327 977
AgeI A'CCGG_T
Cuts at: 5249 5249
Size: 6418
AhdI GACnn_n'nnGTC
Cuts at: 5147 5147
Size: 6418
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6418
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6418
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2630 2662 2630
Size: 32 6386
AlwNI CAG_nnn'CTG
Cuts at: 2216 3013 3442 4161 5733 2216
Size: 797 429 719 1572 2901
Fragments arranged by size:
2901 1572 797 719 429
ApaLI G'TGCA_C
Cuts at: 4475 5631 4475
Size: 1156 5262
ApoI r'AATT_y
Cuts at: 1493 2078 2434 2752 3727 3953 4105 6214 1493
Size: 585 356 318 975 226 152 2109 1697
Fragments arranged by size:
2109 1697 975 585 356 318 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4972 160
Size: 872 3940 1606
Fragments arranged by size:
3940 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6418
AvaI C'yCGr_G
Cuts at: 1870 1943 3743 1870
Size: 73 1800 4545
Fragments arranged by size:
4545 1800 73
BamHI G'GATC_C
Cuts at: 2048 3748 4115 2048
Size: 1700 367 4351
Fragments arranged by size:
4351 1700 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3739 5194 618
Size: 325 217 627 1952 1455 1842
Fragments arranged by size:
1952 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2163 2877 3737 1391
Size: 772 714 860 4072
Fragments arranged by size:
4072 860 772 714
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2505 928
Size: 971 606 4841
Fragments arranged by size:
4841 971 606
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4609 4643 1903
Size: 34 2672 34 3678
Fragments arranged by size:
3678 2672 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2656 4310 5520 2656
Size: 1654 1210 3554
Fragments arranged by size:
3554 1654 1210
BclI T'GATC_A
Cuts at: 1548 2409 1548
Size: 861 5557
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2573 5029 136
Size: 107 122 71 631 605 901 2456 1525
Fragments arranged by size:
2456 1525 901 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6413 6413
Size: 6418
BlpI GC'TnA_GC
Cuts at: 2871 3835 2871
Size: 964 5454
Bme1580I G_kGCm'C
Cuts at: 1790 3357 4479 5635 1790
Size: 1567 1122 1156 2573
Fragments arranged by size:
2573 1567 1156 1122
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6418
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2257 2600 4149
Size: 416 53 83 399 24 840 343 1549
Cuts at: 4149 5107 442
Size: 958 1753
Fragments arranged by size:
1753 1549 958 840 416 399 343 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6418
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2804 5078 1239
Size: 1565 2274 2579
Fragments arranged by size:
2579 2274 1565
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4543 5408 5706 4543
Size: 865 298 5255
Fragments arranged by size:
5255 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2908 5081 882
Size: 617 1409 2173 2219
Fragments arranged by size:
2219 2173 1409 617
BsaAI yAC'GTr
Cuts at: 493 1204 4209 493
Size: 711 3005 2702
Fragments arranged by size:
3005 2702 711
BsaBI GATnn'nnATC
Cuts at: 6412 6412
Size: 6418
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3387 4225 4607 5242
Size: 53 83 186 936 1854 838 382 635
Cuts at: 5242 275
Size: 1451
Fragments arranged by size:
1854 1451 936 838 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4851 5249 5523 5670 6081 4851
Size: 398 274 147 411 5188
Fragments arranged by size:
5188 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6193
Fragments arranged by size:
6193 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6418
BseYI C'CCAG_C
Cuts at: 2695 5621 6117 2695
Size: 2926 496 2996
Fragments arranged by size:
2996 2926 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5845
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3868 4629 5657 1540
Size: 275 186 1867 761 1028 2301
Fragments arranged by size:
2301 1867 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2163 4479 4564 5635 2163
Size: 2316 85 1071 2946
Fragments arranged by size:
2946 2316 1071 85
BsmI GAATG_Cn'
Cuts at: 2266 2530 3929 4022 2266
Size: 264 1399 93 4662
Fragments arranged by size:
4662 1399 264 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2163 2877 3357 3737 4479 4564 5635
Size: 399 373 714 480 380 742 85 1071
Cuts at: 5635 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 714 480 399 380 373
85
BspEI T'CCGG_A
Cuts at: 6081 6081
Size: 6418
BspHI T'CATG_A
Cuts at: 4307 6037 4307
Size: 1730 4688
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3773 844
Size: 2929 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4305 1394
Size: 429 2482 3507
Fragments arranged by size:
3507 2482 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3390 3606 4913 5087 65
Size: 1183 2142 216 1307 174 1396
Fragments arranged by size:
2142 1396 1307 1183 216 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5062 5249 1700
Size: 110 3252 187 2869
Fragments arranged by size:
3252 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3487 96
Size: 3391 3027
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6418
BssSI C'ACGA_G
Cuts at: 4478 5490 4478
Size: 1012 5406
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2429 794
Size: 1635 4783
BstBI TT'CG_AA
Cuts at: 5222 5222
Size: 6418
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6418
BstXI CCAn_nnnn'nTGG
Cuts at: 2656 2656
Size: 6418
BstYI r'GATC_y
Cuts at: 1972 2048 3748 4115 4500 4517 5969 6055 6067
Size: 76 1700 367 385 17 1452 86 12
Cuts at: 6067 6413 1972
Size: 346 1977
Fragments arranged by size:
1977 1700 1452 385 367 346 86 76
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2377 2571 513
Size: 552 1312 194 4360
Fragments arranged by size:
4360 1312 552 194
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5679
Fragments arranged by size:
5679 683 56
BtsI GCAGTG_nn'
Cuts at: 3934 4728 4748 3934
Size: 794 20 5604
Fragments arranged by size:
5604 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6418
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6383
DraI TTT'AAA
Cuts at: 3722 3722
Size: 6418
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5425 809
Size: 507 4109 1802
Fragments arranged by size:
4109 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2055 2439 2581
Size: 54 1425 50 275 18 225 384 142
Cuts at: 2581 3865 4754 6383 8
Size: 1284 889 1629 43
Fragments arranged by size:
1629 1425 1284 889 384 275 225 142
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3865 1537
Size: 275 2053 4090
Fragments arranged by size:
4090 2053 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2721 4348 1353
Size: 1368 1627 3423
Fragments arranged by size:
3423 1627 1368
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5005 5379 5525 387
Size: 1064 138 3416 374 146 1280
Fragments arranged by size:
3416 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2161 2161
Size: 6418
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2804 3576 4481 5078 5865 6133 1239
Size: 393 1172 772 905 597 787 268 1524
Fragments arranged by size:
1524 1172 905 787 772 597 393 268
EcoRI G'AATT_C
Cuts at: 3727 4105 3727
Size: 378 6040
EcoRV GAT'ATC
Cuts at: 3226 3226
Size: 6418
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2735 2735
Size: 6418
FspI TGC'GCA
Cuts at: 4924 4924
Size: 6418
HaeII r_GCGC'y
Cuts at: 1678 5565 1678
Size: 3887 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2223 2255 2373 2405 3215
Size: 32 135 32 651 32 118 32 810
Cuts at: 3215 3247 5059 5091 5133 5165 1373
Size: 32 1812 32 42 32 2626
Fragments arranged by size:
2626 1812 810 651 135 118 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2553 3762 4008 669
Size: 988 281 615 1209 246 3079
Fragments arranged by size:
3079 1209 988 615 281 246
HindIII A'AGCT_T
Cuts at: 748 3558 748
Size: 2810 3608
HpaI GTT'AAC
Cuts at: 2553 4008 2553
Size: 1455 4963
KpnI G_GTAC'C
Cuts at: 3743 3743
Size: 6418
MfeI C'AATT_G
Cuts at: 4017 4017
Size: 6418
MluI A'CGCG_T
Cuts at: 6294 6294
Size: 6418
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5532 5716 1305
Size: 4227 184 2007
Fragments arranged by size:
4227 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2441 2583 10
Size: 54 1425 343 609 142 3845
Fragments arranged by size:
3845 1425 609 343 142 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2333 2355 2654 4376 4735 4894 518
Size: 648 1167 22 299 1722 359 159 2042
Fragments arranged by size:
2042 1722 1167 648 359 299 159 22
MspA1I CmG'CkG
Cuts at: 1606 2749 3834 4511 5659 5904 1606
Size: 1143 1085 677 1148 245 2120
Fragments arranged by size:
2120 1148 1143 1085 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2377 2571 513
Size: 552 1312 194 4360
Fragments arranged by size:
4360 1312 552 194
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6418
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6418
NotI GC'GGCC_GC
Cuts at: 3865 3865
Size: 6418
NspI r_CATG'y
Cuts at: 1132 1229 3301 3776 5321 1132
Size: 97 2072 475 1545 2229
Fragments arranged by size:
2229 2072 1545 475 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6418
PciI A'CATG_T
Cuts at: 3297 5317 3297
Size: 2020 4398
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6418
PfoI T'CCnGG_A
Cuts at: 3330 3330
Size: 6418
PmeI GTTT'AAAC
Cuts at: 3722 3722
Size: 6418
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4487 4519 6000 6032 4487
Size: 32 1481 32 4873
Fragments arranged by size:
4873 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2189 1613
Size: 576 5842
PsiI TTA'TAA
Cuts at: 3479 3988 3479
Size: 509 5909
PstI C_TGCA'G
Cuts at: 830 3770 830
Size: 2940 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6418
PvuII CAG'CTG
Cuts at: 2749 2749
Size: 6418
SacI G_AGCT'C
Cuts at: 2163 2163
Size: 6418
SalI G'TCGA_C
Cuts at: 1936 3760 1936
Size: 1824 4594
SbfI CC_TGCA'GG
Cuts at: 3770 3770
Size: 6418
ScaI AGT'ACT
Cuts at: 2446 4666 6378 2446
Size: 2220 1712 2486
Fragments arranged by size:
2486 2220 1712
SfcI C'TryA_G
Cuts at: 826 1045 3075 3766 4901 5582 5773 826
Size: 219 2030 691 1135 681 191 1471
Fragments arranged by size:
2030 1471 1135 691 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6418
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6418
SmaI CCC'GGG
Cuts at: 1872 3745 1872
Size: 1873 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4522 5423 5685 820
Size: 197 926 2579 901 262 1553
Fragments arranged by size:
2579 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4209 493
Size: 3716 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6418
SphI G_CATG'C
Cuts at: 1132 3776 1132
Size: 2644 3774
SspI AAT'ATT
Cuts at: 5 52 2428 3325 4342 5
Size: 47 2376 897 1017 2081
Fragments arranged by size:
2376 2081 1017 897 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6418
StyI C'CwwG_G
Cuts at: 513 1065 2052 2092 2377 2571 3857 513
Size: 552 987 40 285 194 1286 3074
Fragments arranged by size:
3074 1286 987 552 285 194 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2239 2792 3395 4445 4462 4615 4800
Size: 1255 659 553 603 1050 17 153 185
Cuts at: 4800 5245 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1050 659 603 553 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2444 3487 4664
Size: 274 80 33 51 1443 467 1043 1177
Cuts at: 4664 6376 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1177 1043 467 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6378 6410 6378
Size: 32 6386
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6418
XbaI T'CTAG_A
Cuts at: 3754 3754
Size: 6418
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6418
XmaI C'CCGG_G
Cuts at: 1870 3743 1870
Size: 1873 4545
XmnI GAAnn'nnTTC
Cuts at: 4547 4547
Size: 6418
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4226 276
Size: 53 83 186 936 2692 2468
Fragments arranged by size:
2692 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI