Restriction map for
pFN21AB8671
(Circular) (Six-base) MAPSORT of: pFN21AB8671.seq Check: 915 from: 1 to: 5893
pFN21AB8671 5893 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3703 278
Size: 53 83 186 936 2167 2468
Fragments arranged by size:
2468 2167 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2682 3236 1937
Size: 745 554 4594
Fragments arranged by size:
4594 745 554
Acc65I G'GTAC_C
Cuts at: 3214 3214
Size: 5893
AclI AA'CG_TT
Cuts at: 1563 4020 4393 1563
Size: 2457 373 3063
Fragments arranged by size:
3063 2457 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2130 2444 3060 3956 5340 5608 1632
Size: 498 314 616 896 1384 268 1917
Fragments arranged by size:
1917 1384 896 616 498 314 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5696
AflIII A'CryG_T
Cuts at: 1203 2375 4792 5769 1203
Size: 1172 2417 977 1327
Fragments arranged by size:
2417 1327 1172 977
AgeI A'CCGG_T
Cuts at: 4724 4724
Size: 5893
AhdI GACnn_n'nnGTC
Cuts at: 4622 4622
Size: 5893
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5893
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5893
AlwNI CAG_nnn'CTG
Cuts at: 2320 3636 5208 2320
Size: 1316 1572 3005
Fragments arranged by size:
3005 1572 1316
ApaI G_GGCC'C
Cuts at: 2490 2490
Size: 5893
ApaLI G'TGCA_C
Cuts at: 3950 5106 3950
Size: 1156 4737
ApoI r'AATT_y
Cuts at: 1493 2253 3161 3202 3428 3580 5689 1493
Size: 760 908 41 226 152 2109 1697
Fragments arranged by size:
2109 1697 908 760 226 152 41
AseI AT'TA_AT
Cuts at: 160 1032 4447 160
Size: 872 3415 1606
Fragments arranged by size:
3415 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5893
AvaI C'yCGr_G
Cuts at: 1870 1943 3218 1870
Size: 73 1275 4545
Fragments arranged by size:
4545 1275 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2831 2864 2831
Size: 33 5860
BamHI G'GATC_C
Cuts at: 3223 3590 3223
Size: 367 5526
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3214 4669 618
Size: 325 217 627 1427 1455 1842
Fragments arranged by size:
1842 1455 1427 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2305 2490 3212 1391
Size: 914 185 722 4072
Fragments arranged by size:
4072 914 722 185
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4922
BbvCI CC'TCA_GC
Cuts at: 2832 2832
Size: 5893
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4084 4118 1903
Size: 34 50 34 2063 34 3678
Fragments arranged by size:
3678 2063 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3785 4995 3785
Size: 1210 4683
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5893
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4504 136
Size: 107 122 71 631 605 2832 1525
Fragments arranged by size:
2832 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5888 5888
Size: 5893
BlpI GC'TnA_GC
Cuts at: 3310 3310
Size: 5893
Bme1580I G_kGCm'C
Cuts at: 1790 2490 3954 5110 1790
Size: 700 1464 1156 2573
Fragments arranged by size:
2573 1464 1156 700
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5893
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3624 4582 442
Size: 416 53 83 399 24 2207 958 1753
Fragments arranged by size:
2207 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5893
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4553 1239
Size: 3314 2579
Bpu10I CC'TnA_GC
Cuts at: 2832 2832
Size: 5893
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2795 4018 4883 5181 2795
Size: 1223 865 298 3507
Fragments arranged by size:
3507 1223 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4556 882
Size: 617 3057 2219
Fragments arranged by size:
3057 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2378 3684 493
Size: 711 1174 1306 2702
Fragments arranged by size:
2702 1306 1174 711
BsaBI GATnn'nnATC
Cuts at: 5887 5887
Size: 5893
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3700 4082 4717 275
Size: 53 83 186 936 2167 382 635 1451
Fragments arranged by size:
2167 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4326 4724 4998 5145 5556 4326
Size: 398 274 147 411 4663
Fragments arranged by size:
4663 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2968 2998 1178
Size: 30 165 30 1565 30 4073
Fragments arranged by size:
4073 1565 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2538 2541 2849 2976 1186
Size: 1352 3 308 127 4103
Fragments arranged by size:
4103 1352 308 127 3
BseYI C'CCAG_C
Cuts at: 2897 5096 5592 2897
Size: 2199 496 3198
Fragments arranged by size:
3198 2199 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2152 1163
Size: 573 416 4904
Fragments arranged by size:
4904 573 416
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3343 4104 5132 1540
Size: 275 186 1342 761 1028 2301
Fragments arranged by size:
2301 1342 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2305 3954 4039 5110 2305
Size: 1649 85 1071 3088
Fragments arranged by size:
3088 1649 1071 85
BsmI GAATG_Cn'
Cuts at: 2332 3404 3497 2332
Size: 1072 93 4728
Fragments arranged by size:
4728 1072 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2305 2490 3212 3954 4039 5110 1391
Size: 399 515 185 722 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 722 515 399 185 85
BspEI T'CCGG_A
Cuts at: 5556 5556
Size: 5893
BspHI T'CATG_A
Cuts at: 2614 3782 5512 2614
Size: 1168 1730 2995
Fragments arranged by size:
2995 1730 1168
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3248 844
Size: 2404 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3780 1394
Size: 429 1957 3507
Fragments arranged by size:
3507 1957 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4388 4562 65
Size: 1183 3140 174 1396
Fragments arranged by size:
3140 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4537 4724 1700
Size: 110 2727 187 2869
Fragments arranged by size:
2869 2727 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5893
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5893
BssSI C'ACGA_G
Cuts at: 3953 4965 3953
Size: 1012 4881
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5893
BstBI TT'CG_AA
Cuts at: 4697 4697
Size: 5893
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5893
BstYI r'GATC_y
Cuts at: 1972 2422 3223 3590 3975 3992 5444 5530 5542
Size: 450 801 367 385 17 1452 86 12
Cuts at: 5542 5888 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 801 450 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2988 2988
Size: 5893
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5341
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5154
Fragments arranged by size:
5154 683 56
BtsI GCAGTG_nn'
Cuts at: 2931 3409 4203 4223 2931
Size: 478 794 20 4601
Fragments arranged by size:
4601 794 478 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5893
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5858
DraI TTT'AAA
Cuts at: 3064 3197 3064
Size: 133 5760
DraIII CAC_nnn'GTG
Cuts at: 2873 2873
Size: 5893
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4900 809
Size: 507 3584 1802
Fragments arranged by size:
3584 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3340 4229 5858
Size: 54 1425 50 275 18 1510 889 1629
Cuts at: 5858 8
Size: 43
Fragments arranged by size:
1629 1510 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3340 1537
Size: 275 1528 4090
Fragments arranged by size:
4090 1528 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2448 3823 1353
Size: 1095 1375 3423
Fragments arranged by size:
3423 1375 1095
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4480 4854 5000 387
Size: 1064 138 2891 374 146 1280
Fragments arranged by size:
2891 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2303 2303
Size: 5893
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2130 2444 3060 3956 4553 5340 5608
Size: 393 498 314 616 896 597 787 268
Cuts at: 5608 1239
Size: 1524
Fragments arranged by size:
1524 896 787 616 597 498 393 314
268
EcoO109I rG'GnC_Cy
Cuts at: 2317 2799 2317
Size: 482 5411
EcoRI G'AATT_C
Cuts at: 3202 3580 3202
Size: 378 5515
EcoRV GAT'ATC
Cuts at: 2398 2398
Size: 5893
FspI TGC'GCA
Cuts at: 4399 4399
Size: 5893
HaeII r_GCGC'y
Cuts at: 1678 5040 1678
Size: 3362 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2968 3000 3135 3167 4534
Size: 32 135 32 1396 32 135 32 1367
Cuts at: 4534 4566 4608 4640 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1396 1367 135 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2130 3237 3483 669
Size: 988 281 192 1107 246 3079
Fragments arranged by size:
3079 1107 988 281 246 192
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5893
HpaI GTT'AAC
Cuts at: 2130 3483 2130
Size: 1353 4540
KpnI G_GTAC'C
Cuts at: 3218 3218
Size: 5893
MfeI C'AATT_G
Cuts at: 3492 3492
Size: 5893
MluI A'CGCG_T
Cuts at: 5769 5769
Size: 5893
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2068 2938 5007 5191 1305
Size: 763 870 2069 184 2007
Fragments arranged by size:
2069 2007 870 763 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4071
Fragments arranged by size:
4071 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2346 3851 4210 4369 518
Size: 648 1180 1505 359 159 2042
Fragments arranged by size:
2042 1505 1180 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3309 3986 5134 5379 1606
Size: 1703 677 1148 245 2120
Fragments arranged by size:
2120 1703 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5341
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5893
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5893
NotI GC'GGCC_GC
Cuts at: 3340 3340
Size: 5893
NspI r_CATG'y
Cuts at: 1132 1229 3251 4796 1132
Size: 97 2022 1545 2229
Fragments arranged by size:
2229 2022 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5893
PciI A'CATG_T
Cuts at: 4792 4792
Size: 5893
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2051 2102 1108
Size: 943 51 4899
Fragments arranged by size:
4899 943 51
PmeI GTTT'AAAC
Cuts at: 3197 3197
Size: 5893
PmlI CAC'GTG
Cuts at: 2378 2378
Size: 5893
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3962 3994 5475 5507 3962
Size: 32 1481 32 4348
Fragments arranged by size:
4348 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5893
PsiI TTA'TAA
Cuts at: 3463 3463
Size: 5893
PspOMI G'GGCC_C
Cuts at: 2486 2486
Size: 5893
PstI C_TGCA'G
Cuts at: 830 2938 2998 3245 830
Size: 2108 60 247 3478
Fragments arranged by size:
3478 2108 247 60
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5893
SacI G_AGCT'C
Cuts at: 2305 2305
Size: 5893
SalI G'TCGA_C
Cuts at: 1936 3235 1936
Size: 1299 4594
SapI GCTCTTCn'nnn_
Cuts at: 2448 2448
Size: 5893
SbfI CC_TGCA'GG
Cuts at: 3245 3245
Size: 5893
ScaI AGT'ACT
Cuts at: 4141 5853 4141
Size: 1712 4181
SfcI C'TryA_G
Cuts at: 826 1045 2934 2994 3241 4376 5057 5248 826
Size: 219 1889 60 247 1135 681 191 1471
Fragments arranged by size:
1889 1471 1135 681 247 219 191 60
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5893
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5893
SmaI CCC'GGG
Cuts at: 1872 3220 1872
Size: 1348 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2810 3997 4898 5160 820
Size: 197 926 867 1187 901 262 1553
Fragments arranged by size:
1553 1187 926 901 867 262 197
SnaBI TAC'GTA
Cuts at: 493 3684 493
Size: 3191 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5893
SphI G_CATG'C
Cuts at: 1132 3251 1132
Size: 2119 3774
SspI AAT'ATT
Cuts at: 5 52 3817 5
Size: 47 3765 2081
Fragments arranged by size:
3765 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5893
StyI C'CwwG_G
Cuts at: 513 1065 2802 3332 513
Size: 552 1737 530 3074
Fragments arranged by size:
3074 1737 552 530
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2743 3920 3937 4090 4275 4720 325
Size: 1255 1163 1177 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1177 1163 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3105 4139 5851
Size: 274 80 33 51 1443 1128 1034 1712
Cuts at: 5851 96
Size: 138
Fragments arranged by size:
1712 1443 1128 1034 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2161 2193 5853 5885 2161
Size: 32 3660 32 2169
Fragments arranged by size:
3660 2169 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5893
XbaI T'CTAG_A
Cuts at: 3229 3229
Size: 5893
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5893
XmaI C'CCGG_G
Cuts at: 1870 3218 1870
Size: 1348 4545
XmnI GAAnn'nnTTC
Cuts at: 2457 4022 2457
Size: 1565 4328
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3701 276
Size: 53 83 186 936 2167 2468
Fragments arranged by size:
2468 2167 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I EcoNI FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PpuMI PspXI PsrI PvuII RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI XcmI