Restriction map for
pFN21AB8670
(Circular) (Six-base) MAPSORT of: pFN21AB8670.seq Check: 6199 from: 1 to: 6097
pFN21AB8670 6097 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3907 278
Size: 53 83 186 936 2371 2468
Fragments arranged by size:
2468 2371 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3440 1937
Size: 1503 4594
Acc65I G'GTAC_C
Cuts at: 3418 3418
Size: 6097
AclI AA'CG_TT
Cuts at: 1563 2856 4224 4597 1563
Size: 1293 1368 373 3063
Fragments arranged by size:
3063 1368 1293 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2185 2575 4160 5544 5812 1632
Size: 553 390 1585 1384 268 1917
Fragments arranged by size:
1917 1585 1384 553 390 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5900
AflIII A'CryG_T
Cuts at: 1203 3070 4996 5973 1203
Size: 1867 1926 977 1327
Fragments arranged by size:
1926 1867 1327 977
AgeI A'CCGG_T
Cuts at: 4928 4928
Size: 6097
AhdI GACnn_n'nnGTC
Cuts at: 4826 4826
Size: 6097
AleI CACnn'nnGTG
Cuts at: 1166 2972 1166
Size: 1806 4291
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6097
AlwNI CAG_nnn'CTG
Cuts at: 3840 5412 3840
Size: 1572 4525
ApaI G_GGCC'C
Cuts at: 2256 2262 2256
Size: 6 6091
ApaLI G'TGCA_C
Cuts at: 4154 5310 4154
Size: 1156 4941
ApoI r'AATT_y
Cuts at: 1493 2646 3093 3406 3632 3784 5893 1493
Size: 1153 447 313 226 152 2109 1697
Fragments arranged by size:
2109 1697 1153 447 313 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4651 160
Size: 872 3619 1606
Fragments arranged by size:
3619 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6097
AvaI C'yCGr_G
Cuts at: 1870 1943 2209 2255 3422 1870
Size: 73 266 46 1167 4545
Fragments arranged by size:
4545 1167 266 73 46
BamHI G'GATC_C
Cuts at: 2289 3427 3794 2289
Size: 1138 367 4592
Fragments arranged by size:
4592 1138 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2137 2239 2270 3276 3418
Size: 325 217 627 350 102 31 1006 142
Cuts at: 3418 4873 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1006 627 350 325 217 142
102 31
BanII G_rGCy'C
Cuts at: 1391 2035 2256 2262 2487 2557 3416 1391
Size: 644 221 6 225 70 859 4072
Fragments arranged by size:
4072 859 644 225 221 70 6
BbeI G_GCGC'C
Cuts at: 2274 2274
Size: 6097
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2179 2581 928
Size: 971 280 402 4444
Fragments arranged by size:
4444 971 402 280
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2150 2184 4288 4322 1903
Size: 34 213 34 2104 34 3678
Fragments arranged by size:
3678 2104 213 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3989 5199 3989
Size: 1210 4887
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6097
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4708 136
Size: 107 122 71 631 605 3036 1525
Fragments arranged by size:
3036 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6092 6092
Size: 6097
BlpI GC'TnA_GC
Cuts at: 2051 3514 2051
Size: 1463 4634
Bme1580I G_kGCm'C
Cuts at: 1790 2079 2142 2244 2256 2262 2304 2985 4158
Size: 289 63 102 12 6 42 681 1173
Cuts at: 4158 5314 1790
Size: 1156 2573
Fragments arranged by size:
2573 1173 1156 681 289 102 63 42
12 6
BmgBI CAC'GTC
Cuts at: 1318 3349 1318
Size: 2031 4066
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3828 4786 442
Size: 416 53 83 399 24 2411 958 1753
Fragments arranged by size:
2411 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6097
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2333 3317 4757 1239
Size: 1094 984 1440 2579
Fragments arranged by size:
2579 1440 1094 984
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4222 5087 5385 4222
Size: 865 298 4934
Fragments arranged by size:
4934 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2545 2832 4760 882
Size: 617 1046 287 1928 2219
Fragments arranged by size:
2219 1928 1046 617 287
BsaAI yAC'GTr
Cuts at: 493 1204 2155 3888 493
Size: 711 951 1733 2702
Fragments arranged by size:
2702 1733 951 711
BsaBI GATnn'nnATC
Cuts at: 6091 6091
Size: 6097
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2271 3904 4286 4921
Size: 53 83 186 936 738 1633 382 635
Cuts at: 4921 275
Size: 1451
Fragments arranged by size:
1633 1451 936 738 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4530 4928 5202 5349 5760 4530
Size: 398 274 147 411 4867
Fragments arranged by size:
4867 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2841 2871 3290 3320 1178
Size: 30 165 30 1438 30 419 30 3955
Fragments arranged by size:
3955 1438 419 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6097
BseYI C'CCAG_C
Cuts at: 2027 2038 2065 2218 2243 2830 5300 5796 2027
Size: 11 27 153 25 587 2470 496 2328
Fragments arranged by size:
2470 2328 587 496 153 27 25 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3069 3210 1163
Size: 573 1333 141 4050
Fragments arranged by size:
4050 1333 573 141
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2182 2279 2551 3292 3547 4308
Size: 275 186 181 97 272 741 255 761
Cuts at: 4308 5336 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 761 741 275 272 255 186
181 97
BsiHKAI G_wGCw'C
Cuts at: 2557 4158 4243 5314 2557
Size: 1601 85 1071 3340
Fragments arranged by size:
3340 1601 1071 85
BsmI GAATG_Cn'
Cuts at: 3608 3701 3608
Size: 93 6004
BsmBI CGTCTCn'nnnn_
Cuts at: 2500 2500
Size: 6097
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2035 2079 2142 2244 2256 2262 2304
Size: 399 245 44 63 102 12 6 42
Cuts at: 2304 2487 2557 2985 3416 4158 4243 5314 1391
Size: 183 70 428 431 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 431 428 399 245 183
102 85 70 63 44 42 12 6
BspEI T'CCGG_A
Cuts at: 5760 5760
Size: 6097
BspHI T'CATG_A
Cuts at: 3986 5716 3986
Size: 1730 4367
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2527 2949 3452 844
Size: 1683 422 503 3489
Fragments arranged by size:
3489 1683 503 422
BsrBI CCG'CTC
Cuts at: 1394 1823 2055 3984 1394
Size: 429 232 1929 3507
Fragments arranged by size:
3507 1929 429 232
BsrDI GCAATG_nn'
Cuts at: 65 1248 2624 4592 4766 65
Size: 1183 1376 1968 174 1396
Fragments arranged by size:
1968 1396 1376 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2106 2267 4741 4928 1700
Size: 110 296 161 2474 187 2869
Fragments arranged by size:
2869 2474 296 187 161 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6097
BssHII G'CGCG_C
Cuts at: 1925 2164 1925
Size: 239 5858
BssSI C'ACGA_G
Cuts at: 4157 5169 4157
Size: 1012 5085
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6097
BstBI TT'CG_AA
Cuts at: 4901 4901
Size: 6097
BstEII G'GTnAC_C
Cuts at: 1184 3373 1184
Size: 2189 3908
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2830 2011
Size: 819 5278
BstYI r'GATC_y
Cuts at: 1972 2289 3427 3794 4179 4196 5648 5734 5746
Size: 317 1138 367 385 17 1452 86 12
Cuts at: 5746 6092 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1138 385 367 346 317 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2517 2517
Size: 6097
BtgI C'CryG_G
Cuts at: 513 1065 2004 2142 2671 3314 513
Size: 552 939 138 529 643 3296
Fragments arranged by size:
3296 939 643 552 529 138
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5358
Fragments arranged by size:
5358 683 56
BtsI GCAGTG_nn'
Cuts at: 2360 3057 3613 4407 4427 2360
Size: 697 556 794 20 4030
Fragments arranged by size:
4030 794 697 556 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6097
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2815 2850 3099 3134 3139 3174 529
Size: 35 2251 35 249 35 5 35 3452
Fragments arranged by size:
3452 2251 249 35 35 35 35 5
DraI TTT'AAA
Cuts at: 3401 3401
Size: 6097
DraIII CAC_nnn'GTG
Cuts at: 3373 3373
Size: 6097
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5104 809
Size: 507 3788 1802
Fragments arranged by size:
3788 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2088 2548 2692
Size: 54 1425 50 275 18 258 460 144
Cuts at: 2692 2892 3381 3544 4433 6062 8
Size: 200 489 163 889 1629 43
Fragments arranged by size:
1629 1425 889 489 460 275 258 200
163 144 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2548 3544 1537
Size: 275 736 996 4090
Fragments arranged by size:
4090 996 736 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4027 1353
Size: 2674 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2147 2890 4684 5058 5204 387
Size: 1064 138 558 743 1794 374 146 1280
Fragments arranged by size:
1794 1280 1064 743 558 374 146 138
EcoICRI GAG'CTC
Cuts at: 2555 2555
Size: 6097
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2185 2333 2575 3317 4160 4757 5544
Size: 393 553 148 242 742 843 597 787
Cuts at: 5544 5812 1239
Size: 268 1524
Fragments arranged by size:
1524 843 787 742 597 553 393 268
242 148
EcoO109I rG'GnC_Cy
Cuts at: 2259 2259
Size: 6097
EcoRI G'AATT_C
Cuts at: 3093 3406 3784 3093
Size: 313 378 5406
Fragments arranged by size:
5406 378 313
FspI TGC'GCA
Cuts at: 4603 4603
Size: 6097
HaeII r_GCGC'y
Cuts at: 1678 2012 2098 2219 2274 5244 1678
Size: 334 86 121 55 2970 2531
Fragments arranged by size:
2970 2531 334 121 86 55
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3195 3227 4738 4770 4812
Size: 32 135 32 1623 32 1511 32 42
Cuts at: 4812 4844 1373
Size: 32 2626
Fragments arranged by size:
2626 1623 1511 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2571 3441 3687 669
Size: 988 281 633 870 246 3079
Fragments arranged by size:
3079 988 870 633 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6097
HpaI GTT'AAC
Cuts at: 3687 3687
Size: 6097
KasI G'GCGC_C
Cuts at: 2270 2270
Size: 6097
KpnI G_GTAC'C
Cuts at: 3422 3422
Size: 6097
MfeI C'AATT_G
Cuts at: 3696 3696
Size: 6097
MluI A'CGCG_T
Cuts at: 5973 5973
Size: 6097
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2460 5211 5395 1305
Size: 1155 2751 184 2007
Fragments arranged by size:
2751 2007 1155 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2694 3383 10
Size: 54 1425 343 862 689 2724
Fragments arranged by size:
2724 1425 862 689 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2972 4055 4414 4573 518
Size: 648 1806 1083 359 159 2042
Fragments arranged by size:
2042 1806 1083 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2947 2988 3513 4190 5338 5583 1606
Size: 1341 41 525 677 1148 245 2120
Fragments arranged by size:
2120 1341 1148 677 525 245 41
NaeI GCC'GGC
Cuts at: 2108 2269 2108
Size: 161 5936
NarI GG'CG_CC
Cuts at: 2271 2271
Size: 6097
NcoI C'CATG_G
Cuts at: 513 1065 2004 3314 513
Size: 552 939 1310 3296
Fragments arranged by size:
3296 1310 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6097
NgoMIV G'CCGG_C
Cuts at: 2106 2267 2106
Size: 161 5936
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6097
NotI GC'GGCC_GC
Cuts at: 3544 3544
Size: 6097
NspI r_CATG'y
Cuts at: 1132 1229 3033 3074 3357 3455 5000 1132
Size: 97 1804 41 283 98 1545 2229
Fragments arranged by size:
2229 1804 1545 283 98 97 41
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6097
PciI A'CATG_T
Cuts at: 3070 4996 3070
Size: 1926 4171
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2155 1108
Size: 1047 5050
PfoI T'CCnGG_A
Cuts at: 2099 2099
Size: 6097
PmeI GTTT'AAAC
Cuts at: 3401 3401
Size: 6097
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4166 4198 5679 5711 4166
Size: 32 1481 32 4552
Fragments arranged by size:
4552 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6097
PsiI TTA'TAA
Cuts at: 3667 3667
Size: 6097
PspOMI G'GGCC_C
Cuts at: 2252 2258 2252
Size: 6 6091
PstI C_TGCA'G
Cuts at: 830 2538 2946 3449 830
Size: 1708 408 503 3478
Fragments arranged by size:
3478 1708 503 408
PvuI CG_AT'CG
Cuts at: 2001 2182 2001
Size: 181 5916
PvuII CAG'CTG
Cuts at: 2947 2947
Size: 6097
SacI G_AGCT'C
Cuts at: 2557 2557
Size: 6097
SalI G'TCGA_C
Cuts at: 1936 3439 1936
Size: 1503 4594
SbfI CC_TGCA'GG
Cuts at: 3449 3449
Size: 6097
ScaI AGT'ACT
Cuts at: 4345 6057 4345
Size: 1712 4385
SfcI C'TryA_G
Cuts at: 826 1045 2534 2942 3445 4580 5261 5452 826
Size: 219 1489 408 503 1135 681 191 1471
Fragments arranged by size:
1489 1471 1135 681 503 408 219 191
SfoI GGC'GCC
Cuts at: 2272 2272
Size: 6097
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6097
SgrAI Cr'CCGG_yG
Cuts at: 1700 2267 1700
Size: 567 5530
SmaI CCC'GGG
Cuts at: 1872 2257 3424 1872
Size: 385 1167 4545
Fragments arranged by size:
4545 1167 385
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4201 5102 5364 820
Size: 197 926 2258 901 262 1553
Fragments arranged by size:
2258 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3888 493
Size: 3395 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6097
SphI G_CATG'C
Cuts at: 1132 3033 3455 1132
Size: 1901 422 3774
Fragments arranged by size:
3774 1901 422
SrfI GCCC'GGGC
Cuts at: 2257 2257
Size: 6097
SspI AAT'ATT
Cuts at: 5 52 4021 5
Size: 47 3969 2081
Fragments arranged by size:
3969 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6097
StyI C'CwwG_G
Cuts at: 513 1065 2004 3314 3536 513
Size: 552 939 1310 222 3074
Fragments arranged by size:
3074 1310 939 552 222
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2822 3163 3278 4124 4141 4294 4479
Size: 1255 1242 341 115 846 17 153 185
Cuts at: 4479 4924 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1242 846 445 341 185 153
115 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4343 6055 96
Size: 274 80 33 51 1443 2366 1712 138
Fragments arranged by size:
2366 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2616 2648 6057 6089 2616
Size: 32 3409 32 2624
Fragments arranged by size:
3409 2624 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6097
XbaI T'CTAG_A
Cuts at: 3433 3433
Size: 6097
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2711 2011
Size: 700 5397
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6097
XmaI C'CCGG_G
Cuts at: 1870 2255 3422 1870
Size: 385 1167 4545
Fragments arranged by size:
4545 1167 385
XmnI GAAnn'nnTTC
Cuts at: 2605 3141 4226 2605
Size: 536 1085 4476
Fragments arranged by size:
4476 1085 536
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3905 276
Size: 53 83 186 936 2371 2468
Fragments arranged by size:
2468 2371 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BstZ17I EcoNI EcoRV FalI FseI
FspAI NruI NsiI PacI PmlI PpuMI PspXI PsrI
RsrII SacII SanDI SapI SexAI SfiI SwaI