Restriction map for
pFN21AB8669
(Circular) (Six-base) MAPSORT of: pFN21AB8669.seq Check: 8225 from: 1 to: 6475
pFN21AB8669 6475 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2764 2764
Size: 6475
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4285 278
Size: 53 83 186 936 2749 2468
Fragments arranged by size:
2749 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3818 1937
Size: 1881 4594
Acc65I G'GTAC_C
Cuts at: 2679 2928 3796 2679
Size: 249 868 5358
Fragments arranged by size:
5358 868 249
AclI AA'CG_TT
Cuts at: 1563 4602 4975 1563
Size: 3039 373 3063
Fragments arranged by size:
3063 3039 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3139 3470 4538 5922 6190 1632
Size: 1507 331 1068 1384 268 1917
Fragments arranged by size:
1917 1507 1384 1068 331 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6278
AflIII A'CryG_T
Cuts at: 1203 5374 6351 1203
Size: 4171 977 1327
Fragments arranged by size:
4171 1327 977
AgeI A'CCGG_T
Cuts at: 5306 5306
Size: 6475
AhdI GACnn_n'nnGTC
Cuts at: 5204 5204
Size: 6475
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6475
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6475
AlwNI CAG_nnn'CTG
Cuts at: 2513 2591 2716 2876 2974 4218 5790 2513
Size: 78 125 160 98 1244 1572 3198
Fragments arranged by size:
3198 1572 1244 160 125 98 78
ApaI G_GGCC'C
Cuts at: 2870 3620 2870
Size: 750 5725
ApaLI G'TGCA_C
Cuts at: 3068 4532 5688 3068
Size: 1464 1156 3855
Fragments arranged by size:
3855 1464 1156
ApoI r'AATT_y
Cuts at: 1493 3784 4010 4162 6271 1493
Size: 2291 226 152 2109 1697
Fragments arranged by size:
2291 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5029 160
Size: 872 3997 1606
Fragments arranged by size:
3997 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6475
AvaI C'yCGr_G
Cuts at: 1870 1943 2136 3527 3800 1870
Size: 73 193 1391 273 4545
Fragments arranged by size:
4545 1391 273 193 73
AvrII C'CTAG_G
Cuts at: 2302 2302
Size: 6475
BamHI G'GATC_C
Cuts at: 2961 3805 4172 2961
Size: 844 367 5264
Fragments arranged by size:
5264 844 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2100 2245 2346 2665 2679
Size: 325 217 627 313 145 101 319 14
Cuts at: 2679 2896 2928 3118 3342 3796 5251 618
Size: 217 32 190 224 454 1455 1842
Fragments arranged by size:
1842 1455 627 454 325 319 313 224
217 217 190 145 101 32 14
BanII G_rGCy'C
Cuts at: 1391 2481 2870 3309 3552 3620 3794 1391
Size: 1090 389 439 243 68 174 4072
Fragments arranged by size:
4072 1090 439 389 243 174 68
BbeI G_GCGC'C
Cuts at: 3346 3346
Size: 6475
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3083 3085 928
Size: 971 1184 2 4318
Fragments arranged by size:
4318 1184 971 2
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4666 4700 1903
Size: 34 2729 34 3678
Fragments arranged by size:
3678 2729 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2514 4367 5577 2514
Size: 1853 1210 3412
Fragments arranged by size:
3412 1853 1210
BclI T'GATC_A
Cuts at: 1548 3429 1548
Size: 1881 4594
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2334 2370 2847
Size: 107 122 71 631 605 662 36 477
Cuts at: 2847 3134 3615 5086 136
Size: 287 481 1471 1525
Fragments arranged by size:
1525 1471 662 631 605 481 477 287
122 107 71 36
BglII A'GATC_T
Cuts at: 3024 3295 6470 3024
Size: 271 3175 3029
Fragments arranged by size:
3175 3029 271
BlpI GC'TnA_GC
Cuts at: 2433 3551 3892 2433
Size: 1118 341 5016
Fragments arranged by size:
5016 1118 341
Bme1580I G_kGCm'C
Cuts at: 1790 2044 2250 2349 2638 2870 2899 3072 3123
Size: 254 206 99 289 232 29 173 51
Cuts at: 3123 3588 3620 4536 5692 1790
Size: 465 32 916 1156 2573
Fragments arranged by size:
2573 1156 916 465 289 254 232 206
173 99 51 32 29
BmgBI CAC'GTC
Cuts at: 1318 3520 1318
Size: 2202 4273
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2607 2625 2632
Size: 416 53 83 399 24 1190 18 7
Cuts at: 2632 3661 4206 5164 442
Size: 1029 545 958 1753
Fragments arranged by size:
1753 1190 1029 958 545 416 399 83
53 24 18 7
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6475
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2587 3298 2587
Size: 711 5764
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2462 2799 2898 3245 3280 5135 1239
Size: 1223 337 99 347 35 1855 2579
Fragments arranged by size:
2579 1855 1223 347 337 99 35
Bpu10I CC'TnA_GC
Cuts at: 2475 2562 2475
Size: 87 6388
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4600 5465 5763 4600
Size: 865 298 5312
Fragments arranged by size:
5312 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2059 5138 882
Size: 617 560 3079 2219
Fragments arranged by size:
3079 2219 617 560
BsaAI yAC'GTr
Cuts at: 493 1204 3415 4266 493
Size: 711 2211 851 2702
Fragments arranged by size:
2702 2211 851 711
BsaBI GATnn'nnATC
Cuts at: 6469 6469
Size: 6475
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3343 3445 4282 4664
Size: 53 83 186 936 1810 102 837 382
Cuts at: 4664 5299 275
Size: 635 1451
Fragments arranged by size:
1810 1451 936 837 635 382 186 102
83 53
BsaWI w'CCGG_w
Cuts at: 4908 5306 5580 5727 6138 4908
Size: 398 274 147 411 5245
Fragments arranged by size:
5245 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6250
Fragments arranged by size:
6250 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2759 1186
Size: 1573 4902
BseYI C'CCAG_C
Cuts at: 2241 2383 2534 2683 2706 2724 2735 5678 6174
Size: 142 151 149 23 18 11 2943 496
Cuts at: 6174 2241
Size: 2542
Fragments arranged by size:
2943 2542 496 151 149 142 23 18
11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3134 1163
Size: 573 1398 4504
Fragments arranged by size:
4504 1398 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3339 3643 3925 4686 5714 1540
Size: 275 186 1338 304 282 761 1028 2301
Fragments arranged by size:
2301 1338 1028 761 304 282 275 186
BsiHKAI G_wGCw'C
Cuts at: 3072 3309 3651 3693 4536 4621 5692 3072
Size: 237 342 42 843 85 1071 3855
Fragments arranged by size:
3855 1071 843 342 237 85 42
BsmI GAATG_Cn'
Cuts at: 3986 4079 3986
Size: 93 6382
BsmBI CGTCTCn'nnnn_
Cuts at: 3095 3095
Size: 6475
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2044 2250 2349 2481 2638 2870 2899
Size: 399 254 206 99 132 157 232 29
Cuts at: 2899 3072 3123 3309 3552 3588 3620 3651 3693
Size: 173 51 186 243 36 32 31 42
Cuts at: 3693 3794 4536 4621 5692 1391
Size: 101 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 254 243 232 206
186 173 157 132 101 99 85 51
42 36 32 31 29
BspEI T'CCGG_A
Cuts at: 6138 6138
Size: 6475
BspHI T'CATG_A
Cuts at: 4364 6094 4364
Size: 1730 4745
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2641 2764 3039 3830 844
Size: 1797 123 275 791 3489
Fragments arranged by size:
3489 1797 791 275 123
BsrBI CCG'CTC
Cuts at: 1394 1823 2859 3256 4362 1394
Size: 429 1036 397 1106 3507
Fragments arranged by size:
3507 1106 1036 429 397
BsrDI GCAATG_nn'
Cuts at: 65 1248 4970 5144 65
Size: 1183 3722 174 1396
Fragments arranged by size:
3722 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2034 2850 5119 5306 1700
Size: 110 224 816 2269 187 2869
Fragments arranged by size:
2869 2269 816 224 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6475
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6475
BssSI C'ACGA_G
Cuts at: 4535 5547 4535
Size: 1012 5463
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6475
BstBI TT'CG_AA
Cuts at: 5279 5279
Size: 6475
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6475
BstXI CCAn_nnnn'nTGG
Cuts at: 2837 2837
Size: 6475
BstYI r'GATC_y
Cuts at: 1972 2961 3024 3295 3805 4172 4557 4574 6026
Size: 989 63 271 510 367 385 17 1452
Cuts at: 6026 6112 6124 6470 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 989 510 385 367 346 271
86 63 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3333 3561 3684 513
Size: 552 2268 228 123 3304
Fragments arranged by size:
3304 2268 552 228 123
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3398 3541 487
Size: 683 56 2172 143 3421
Fragments arranged by size:
3421 2172 683 143 56
BtsI GCAGTG_nn'
Cuts at: 2280 2532 2746 3991 4785 4805 2280
Size: 252 214 1245 794 20 3950
Fragments arranged by size:
3950 1245 794 252 214 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6475
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6440
DraI TTT'AAA
Cuts at: 3779 3779
Size: 6475
DraIII CAC_nnn'GTG
Cuts at: 2903 2903
Size: 6475
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3017 5482 809
Size: 507 1701 2465 1802
Fragments arranged by size:
2465 1802 1701 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2371 2529 2848
Size: 54 1425 50 275 18 541 158 319
Cuts at: 2848 2905 3126 3199 3231 3336 3640 3666 3922
Size: 57 221 73 32 105 304 26 256
Cuts at: 3922 4811 6440 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 541 319 304 275 256
221 158 105 73 57 54 50 43
32 26 18
EagI C'GGCC_G
Cuts at: 1537 1812 3336 3640 3922 1537
Size: 275 1524 304 282 4090
Fragments arranged by size:
4090 1524 304 282 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2225 4405 1353
Size: 872 2180 3423
Fragments arranged by size:
3423 2180 872
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2352 2869 5062 5436 5582 387
Size: 1064 138 763 517 2193 374 146 1280
Fragments arranged by size:
2193 1280 1064 763 517 374 146 138
EcoICRI GAG'CTC
Cuts at: 3307 3307
Size: 6475
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2462 2799 2898 3139 3245 3280 3470
Size: 393 830 337 99 241 106 35 190
Cuts at: 3470 4538 5135 5922 6190 1239
Size: 1068 597 787 268 1524
Fragments arranged by size:
1524 1068 830 787 597 393 337 268
241 190 106 99 35
EcoO109I rG'GnC_Cy
Cuts at: 2306 2866 2971 3617 2306
Size: 560 105 646 5164
Fragments arranged by size:
5164 646 560 105
EcoRI G'AATT_C
Cuts at: 3784 4162 3784
Size: 378 6097
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3314 3314
Size: 6475
FspI TGC'GCA
Cuts at: 2294 4981 2294
Size: 2687 3788
HaeII r_GCGC'y
Cuts at: 1678 2752 3346 3509 5622 1678
Size: 1074 594 163 2113 2531
Fragments arranged by size:
2531 2113 1074 594 163
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2483 2515 3164 3196 5116
Size: 32 135 32 911 32 649 32 1920
Cuts at: 5116 5148 5190 5222 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1920 911 649 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3819 4065 669
Size: 988 281 1881 246 3079
Fragments arranged by size:
3079 1881 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6475
HpaI GTT'AAC
Cuts at: 4065 4065
Size: 6475
KasI G'GCGC_C
Cuts at: 3342 3342
Size: 6475
KpnI G_GTAC'C
Cuts at: 2683 2932 3800 2683
Size: 249 868 5358
Fragments arranged by size:
5358 868 249
MfeI C'AATT_G
Cuts at: 4074 4074
Size: 6475
MluI A'CGCG_T
Cuts at: 6351 6351
Size: 6475
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3021 5589 5773 1305
Size: 1716 2568 184 2007
Fragments arranged by size:
2568 2007 1716 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2373 3668 10
Size: 54 1425 343 541 1295 2817
Fragments arranged by size:
2817 1425 1295 541 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2159 2400 2406 3560 4433 4792 4951
Size: 648 993 241 6 1154 873 359 159
Cuts at: 4951 518
Size: 2042
Fragments arranged by size:
2042 1154 993 873 648 359 241 159
6
MspA1I CmG'CkG
Cuts at: 1606 2534 2950 3891 4568 5716 5961 1606
Size: 928 416 941 677 1148 245 2120
Fragments arranged by size:
2120 1148 941 928 677 416 245
NaeI GCC'GGC
Cuts at: 2036 2852 2036
Size: 816 5659
NarI GG'CG_CC
Cuts at: 3343 3343
Size: 6475
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5923
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6475
NgoMIV G'CCGG_C
Cuts at: 2034 2850 2034
Size: 816 5659
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6475
NotI GC'GGCC_GC
Cuts at: 3922 3922
Size: 6475
NspI r_CATG'y
Cuts at: 1132 1229 3833 5378 1132
Size: 97 2604 1545 2229
Fragments arranged by size:
2604 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6475
PciI A'CATG_T
Cuts at: 5374 5374
Size: 6475
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2876 2953 1108
Size: 1768 77 4630
Fragments arranged by size:
4630 1768 77
PfoI T'CCnGG_A
Cuts at: 2228 3222 2228
Size: 994 5481
PmeI GTTT'AAAC
Cuts at: 3779 3779
Size: 6475
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4544 4576 6057 6089 4544
Size: 32 1481 32 4930
Fragments arranged by size:
4930 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6475
PsiI TTA'TAA
Cuts at: 4045 4045
Size: 6475
PspOMI G'GGCC_C
Cuts at: 2866 3616 2866
Size: 750 5725
PstI C_TGCA'G
Cuts at: 830 2518 2970 3827 830
Size: 1688 452 857 3478
Fragments arranged by size:
3478 1688 857 452
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6475
PvuII CAG'CTG
Cuts at: 2950 2950
Size: 6475
SacI G_AGCT'C
Cuts at: 3309 3309
Size: 6475
SalI G'TCGA_C
Cuts at: 1936 3817 1936
Size: 1881 4594
SbfI CC_TGCA'GG
Cuts at: 2970 3827 2970
Size: 857 5618
ScaI AGT'ACT
Cuts at: 4723 6435 4723
Size: 1712 4763
SexAI A'CCwGG_T
Cuts at: 2675 2675
Size: 6475
SfcI C'TryA_G
Cuts at: 826 1045 2514 2966 3823 4958 5639 5830 826
Size: 219 1469 452 857 1135 681 191 1471
Fragments arranged by size:
1471 1469 1135 857 681 452 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2370 2847 3134 2370
Size: 477 287 5711
Fragments arranged by size:
5711 477 287
SfoI GGC'GCC
Cuts at: 3344 3344
Size: 6475
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6475
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6475
SmaI CCC'GGG
Cuts at: 1872 2138 3802 1872
Size: 266 1664 4545
Fragments arranged by size:
4545 1664 266
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4579 5480 5742 820
Size: 197 926 2636 901 262 1553
Fragments arranged by size:
2636 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4266 493
Size: 3773 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6475
SphI G_CATG'C
Cuts at: 1132 3833 1132
Size: 2701 3774
SspI AAT'ATT
Cuts at: 5 52 4399 5
Size: 47 4347 2081
Fragments arranged by size:
4347 2081 47
StuI AGG'CCT
Cuts at: 1517 2328 3422 1517
Size: 811 1094 4570
Fragments arranged by size:
4570 1094 811
StyI C'CwwG_G
Cuts at: 513 1065 2302 2988 3063 3914 513
Size: 552 1237 686 75 851 3074
Fragments arranged by size:
3074 1237 851 686 552 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2133 2164 2198 2233 2357 2683 2720
Size: 1255 553 31 34 35 124 326 37
Cuts at: 2720 4502 4519 4672 4857 5302 325
Size: 1782 17 153 185 445 1498
Fragments arranged by size:
1782 1498 1255 553 445 326 185 153
124 37 35 34 31 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4721 6433 96
Size: 274 80 33 51 1443 2744 1712 138
Fragments arranged by size:
2744 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6435 6467 6435
Size: 32 6443
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6475
XbaI T'CTAG_A
Cuts at: 3811 3811
Size: 6475
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2357 2357
Size: 6475
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6475
XmaI C'CCGG_G
Cuts at: 1870 2136 3800 1870
Size: 266 1664 4545
Fragments arranged by size:
4545 1664 266
XmnI GAAnn'nnTTC
Cuts at: 4604 4604
Size: 6475
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4283 276
Size: 53 83 186 936 2749 2468
Fragments arranged by size:
2749 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbvCI BfrBI BsiWI BstZ17I
Bsu36I EcoNI EcoRV FseI FspAI NruI NsiI PacI
PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI
SrfI SwaI