Restriction map for
pFN21AB8667
(Circular) (Six-base) MAPSORT of: pFN21AB8667.seq Check: 2464 from: 1 to: 5977
pFN21AB8667 5977 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3787 278
Size: 53 83 186 936 2251 2468
Fragments arranged by size:
2468 2251 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2442 3320 1937
Size: 505 878 4594
Fragments arranged by size:
4594 878 505
Acc65I G'GTAC_C
Cuts at: 3298 3298
Size: 5977
AclI AA'CG_TT
Cuts at: 1563 4104 4477 1563
Size: 2541 373 3063
Fragments arranged by size:
3063 2541 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2672 4040 5424 5692 1632
Size: 1040 1368 1384 268 1917
Fragments arranged by size:
1917 1384 1368 1040 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5780
AflIII A'CryG_T
Cuts at: 1203 4876 5853 1203
Size: 3673 977 1327
Fragments arranged by size:
3673 1327 977
AgeI A'CCGG_T
Cuts at: 4808 4808
Size: 5977
AhdI GACnn_n'nnGTC
Cuts at: 4706 4706
Size: 5977
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5977
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5977
AlwNI CAG_nnn'CTG
Cuts at: 2188 3720 5292 2188
Size: 1532 1572 2873
Fragments arranged by size:
2873 1572 1532
ApaI G_GGCC'C
Cuts at: 3233 3233
Size: 5977
ApaLI G'TGCA_C
Cuts at: 4034 5190 4034
Size: 1156 4821
ApoI r'AATT_y
Cuts at: 1493 2426 2772 3286 3512 3664 5773 1493
Size: 933 346 514 226 152 2109 1697
Fragments arranged by size:
2109 1697 933 514 346 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4531 160
Size: 872 3499 1606
Fragments arranged by size:
3499 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5977
AvaI C'yCGr_G
Cuts at: 1870 1943 3302 1870
Size: 73 1359 4545
Fragments arranged by size:
4545 1359 73
BamHI G'GATC_C
Cuts at: 2365 2729 3307 3674 2365
Size: 364 578 367 4668
Fragments arranged by size:
4668 578 367 364
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3298 4753 618
Size: 325 217 627 1511 1455 1842
Fragments arranged by size:
1842 1511 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2205 3233 3296 1391
Size: 814 1028 63 4072
Fragments arranged by size:
4072 1028 814 63
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2867 928
Size: 971 968 4038
Fragments arranged by size:
4038 971 968
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3047 3081 4168 4202 1903
Size: 34 1110 34 1087 34 3678
Fragments arranged by size:
3678 1110 1087 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3869 5079 3869
Size: 1210 4767
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5977
BfrBI ATG'CAT
Cuts at: 2561 2561
Size: 5977
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4588 136
Size: 107 122 71 631 605 337 2579 1525
Fragments arranged by size:
2579 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5972 5972
Size: 5977
BlpI GC'TnA_GC
Cuts at: 3394 3394
Size: 5977
Bme1580I G_kGCm'C
Cuts at: 1790 2159 3233 4038 5194 1790
Size: 369 1074 805 1156 2573
Fragments arranged by size:
2573 1156 1074 805 369
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5977
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2055 3708 4666
Size: 416 53 83 399 24 638 1653 958
Cuts at: 4666 442
Size: 1753
Fragments arranged by size:
1753 1653 958 638 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5977
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2904 2904
Size: 5977
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3147 4637 1239
Size: 1908 1490 2579
Fragments arranged by size:
2579 1908 1490
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3175 4102 4967 5265 3175
Size: 927 865 298 3887
Fragments arranged by size:
3887 927 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4640 882
Size: 617 3141 2219
Fragments arranged by size:
3141 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3768 493
Size: 711 2564 2702
Fragments arranged by size:
2702 2564 711
BsaBI GATnn'nnATC
Cuts at: 5971 5971
Size: 5977
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3784 4166 4801 275
Size: 53 83 186 936 2251 382 635 1451
Fragments arranged by size:
2251 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4410 4808 5082 5229 5640 4410
Size: 398 274 147 411 4747
Fragments arranged by size:
4747 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5752
Fragments arranged by size:
5752 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2110 2279 1186
Size: 924 169 4884
Fragments arranged by size:
4884 924 169
BseYI C'CCAG_C
Cuts at: 2206 3226 5180 5676 2206
Size: 1020 1954 496 2507
Fragments arranged by size:
2507 1954 1020 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2838 1163
Size: 573 1102 4302
Fragments arranged by size:
4302 1102 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2165 3427 4188 5216 1540
Size: 275 186 164 1262 761 1028 2301
Fragments arranged by size:
2301 1262 1028 761 275 186 164
BsiHKAI G_wGCw'C
Cuts at: 4038 4123 5194 4038
Size: 85 1071 4821
Fragments arranged by size:
4821 1071 85
BsmI GAATG_Cn'
Cuts at: 2577 3488 3581 2577
Size: 911 93 4973
Fragments arranged by size:
4973 911 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2796 2796
Size: 5977
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2159 2205 3233 3296 4038 4123 5194
Size: 399 369 46 1028 63 742 85 1071
Cuts at: 5194 1391
Size: 2174
Fragments arranged by size:
2174 1071 1028 742 399 369 85 63
46
BspEI T'CCGG_A
Cuts at: 5640 5640
Size: 5977
BspHI T'CATG_A
Cuts at: 3866 5596 3866
Size: 1730 4247
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2030 3332 844
Size: 1186 1302 3489
Fragments arranged by size:
3489 1302 1186
BsrBI CCG'CTC
Cuts at: 1394 1823 3189 3864 1394
Size: 429 1366 675 3507
Fragments arranged by size:
3507 1366 675 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2148 4472 4646 65
Size: 1183 900 2324 174 1396
Fragments arranged by size:
2324 1396 1183 900 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4621 4808 1700
Size: 110 2811 187 2869
Fragments arranged by size:
2869 2811 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5977
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5977
BssSI C'ACGA_G
Cuts at: 4037 5049 4037
Size: 1012 4965
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5977
BstBI TT'CG_AA
Cuts at: 4781 4781
Size: 5977
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5977
BstXI CCAn_nnnn'nTGG
Cuts at: 3226 3226
Size: 5977
BstYI r'GATC_y
Cuts at: 1972 2365 2729 3202 3307 3674 4059 4076 5528
Size: 393 364 473 105 367 385 17 1452
Cuts at: 5528 5614 5626 5972 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 473 393 385 367 364 346
105 86 17 12
BstZ17I GTA'TAC
Cuts at: 2443 2443
Size: 5977
BtgI C'CryG_G
Cuts at: 513 1065 2004 2869 513
Size: 552 939 865 3621
Fragments arranged by size:
3621 939 865 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5238
Fragments arranged by size:
5238 683 56
BtsI GCAGTG_nn'
Cuts at: 2019 2061 3493 4287 4307 2019
Size: 42 1432 794 20 3689
Fragments arranged by size:
3689 1432 794 42 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5977
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5942
DraI TTT'AAA
Cuts at: 3118 3281 3118
Size: 163 5814
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4984 809
Size: 507 3668 1802
Fragments arranged by size:
3668 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3249 3424 4313
Size: 54 1425 50 275 18 1419 175 889
Cuts at: 4313 5942 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1419 889 275 175 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3424 1537
Size: 275 1612 4090
Fragments arranged by size:
4090 1612 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2303 3907 1353
Size: 950 1604 3423
Fragments arranged by size:
3423 1604 950
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3205 4564 4938 5084 387
Size: 1064 138 1616 1359 374 146 1280
Fragments arranged by size:
1616 1359 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2672 3147 4040 4637 5424 5692 1239
Size: 393 1040 475 893 597 787 268 1524
Fragments arranged by size:
1524 1040 893 787 597 475 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2102 2102
Size: 5977
EcoRI G'AATT_C
Cuts at: 2772 3286 3664 2772
Size: 514 378 5085
Fragments arranged by size:
5085 514 378
FspI TGC'GCA
Cuts at: 4483 4483
Size: 5977
HaeII r_GCGC'y
Cuts at: 1678 5124 1678
Size: 3446 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2086 2118 2615 2647 4618
Size: 32 135 32 514 32 497 32 1971
Cuts at: 4618 4650 4692 4724 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1971 514 497 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3321 3567 669
Size: 988 281 1383 246 3079
Fragments arranged by size:
3079 1383 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5977
HpaI GTT'AAC
Cuts at: 3567 3567
Size: 5977
KpnI G_GTAC'C
Cuts at: 3302 3302
Size: 5977
MfeI C'AATT_G
Cuts at: 3576 3576
Size: 5977
MluI A'CGCG_T
Cuts at: 5853 5853
Size: 5977
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2224 5091 5275 1305
Size: 919 2867 184 2007
Fragments arranged by size:
2867 2007 919 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4155
Fragments arranged by size:
4155 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2553 2794 2909 3935 4294 4453 518
Size: 648 1387 241 115 1026 359 159 2042
Fragments arranged by size:
2042 1387 1026 648 359 241 159 115
MspA1I CmG'CkG
Cuts at: 1606 2130 2185 3393 4070 5218 5463 1606
Size: 524 55 1208 677 1148 245 2120
Fragments arranged by size:
2120 1208 1148 677 524 245 55
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4486
Fragments arranged by size:
4486 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5977
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5977
NotI GC'GGCC_GC
Cuts at: 3424 3424
Size: 5977
NsiI A_TGCA'T
Cuts at: 2563 2563
Size: 5977
NspI r_CATG'y
Cuts at: 1132 1229 3335 4880 1132
Size: 97 2106 1545 2229
Fragments arranged by size:
2229 2106 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5977
PciI A'CATG_T
Cuts at: 4876 4876
Size: 5977
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3239 1108
Size: 2131 3846
PmeI GTTT'AAAC
Cuts at: 3281 3281
Size: 5977
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4046 4078 5559 5591 4046
Size: 32 1481 32 4432
Fragments arranged by size:
4432 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2102 2102
Size: 5977
PshAI GACnn'nnGTC
Cuts at: 1613 2143 1613
Size: 530 5447
PsiI TTA'TAA
Cuts at: 3547 3547
Size: 5977
PspOMI G'GGCC_C
Cuts at: 3229 3229
Size: 5977
PstI C_TGCA'G
Cuts at: 830 2056 3329 830
Size: 1226 1273 3478
Fragments arranged by size:
3478 1273 1226
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5977
PvuII CAG'CTG
Cuts at: 2185 2185
Size: 5977
SalI G'TCGA_C
Cuts at: 1936 3319 1936
Size: 1383 4594
SapI GCTCTTCn'nnn_
Cuts at: 2303 2303
Size: 5977
SbfI CC_TGCA'GG
Cuts at: 3329 3329
Size: 5977
ScaI AGT'ACT
Cuts at: 2044 4225 5937 2044
Size: 2181 1712 2084
Fragments arranged by size:
2181 2084 1712
SfcI C'TryA_G
Cuts at: 826 1045 2052 3325 4460 5141 5332 826
Size: 219 1007 1273 1135 681 191 1471
Fragments arranged by size:
1471 1273 1135 1007 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5977
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5977
SmaI CCC'GGG
Cuts at: 1872 3304 1872
Size: 1432 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3190 4081 4982 5244 820
Size: 197 926 1247 891 901 262 1553
Fragments arranged by size:
1553 1247 926 901 891 262 197
SnaBI TAC'GTA
Cuts at: 493 3768 493
Size: 3275 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5977
SphI G_CATG'C
Cuts at: 1132 3335 1132
Size: 2203 3774
SspI AAT'ATT
Cuts at: 5 52 2281 2470 3901 5
Size: 47 2229 189 1431 2081
Fragments arranged by size:
2229 2081 1431 189 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5977
StyI C'CwwG_G
Cuts at: 513 1065 2004 3416 513
Size: 552 939 1412 3074
Fragments arranged by size:
3074 1412 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4004 4021 4174 4359 4804 325
Size: 1255 2424 17 153 185 445 1498
Fragments arranged by size:
2424 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2042 4223 5935
Size: 274 80 33 51 1443 65 2181 1712
Cuts at: 5935 96
Size: 138
Fragments arranged by size:
2181 1712 1443 274 138 80 65 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2910 2942 5937 5969 2910
Size: 32 2995 32 2918
Fragments arranged by size:
2995 2918 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5977
XbaI T'CTAG_A
Cuts at: 3313 3313
Size: 5977
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2225 2011
Size: 214 5763
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5977
XmaI C'CCGG_G
Cuts at: 1870 3302 1870
Size: 1432 4545
XmnI GAAnn'nnTTC
Cuts at: 3134 4106 3134
Size: 972 5005
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3785 276
Size: 53 83 186 936 2251 2468
Fragments arranged by size:
2468 2251 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
Bpu10I BsiWI Bsu36I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI SwaI