Restriction map for
pFN21AB8664
(Circular) (Six-base) MAPSORT of: pFN21AB8664.seq Check: 3429 from: 1 to: 5932
pFN21AB8664 5932 bp
With 182 enzymes: SgfI *
December 28, 2011 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2167 3742 278
Size: 53 83 186 936 631 1575 2468
Fragments arranged by size:
2468 1575 936 631 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3069 3153 3275 1937
Size: 1132 84 122 4594
Fragments arranged by size:
4594 1132 122 84
Acc65I G'GTAC_C
Cuts at: 3253 3253
Size: 5932
AclI AA'CG_TT
Cuts at: 1563 4059 4432 1563
Size: 2496 373 3063
Fragments arranged by size:
3063 2496 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2857 2954 3995 5379 5647 1632
Size: 1225 97 1041 1384 268 1917
Fragments arranged by size:
1917 1384 1225 1041 268 97
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5735
AflIII A'CryG_T
Cuts at: 1203 4831 5808 1203
Size: 3628 977 1327
Fragments arranged by size:
3628 1327 977
AgeI A'CCGG_T
Cuts at: 4763 4763
Size: 5932
AhdI GACnn_n'nnGTC
Cuts at: 4661 4661
Size: 5932
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5932
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5932
AlwNI CAG_nnn'CTG
Cuts at: 3675 5247 3675
Size: 1572 4360
ApaI G_GGCC'C
Cuts at: 3191 3191
Size: 5932
ApaLI G'TGCA_C
Cuts at: 3989 5145 3989
Size: 1156 4776
ApoI r'AATT_y
Cuts at: 1493 2324 2510 3241 3467 3619 5728 1493
Size: 831 186 731 226 152 2109 1697
Fragments arranged by size:
2109 1697 831 731 226 186 152
AseI AT'TA_AT
Cuts at: 160 1032 4486 160
Size: 872 3454 1606
Fragments arranged by size:
3454 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5932
AvaI C'yCGr_G
Cuts at: 1870 1943 2969 3257 1870
Size: 73 1026 288 4545
Fragments arranged by size:
4545 1026 288 73
BamHI G'GATC_C
Cuts at: 3262 3629 3262
Size: 367 5565
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2369 3253 4708 618
Size: 325 217 627 582 884 1455 1842
Fragments arranged by size:
1842 1455 884 627 582 325 217
BanII G_rGCy'C
Cuts at: 1391 2441 2801 2977 3138 3191 3251 1391
Size: 1050 360 176 161 53 60 4072
Fragments arranged by size:
4072 1050 360 176 161 60 53
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2686 2996 928
Size: 971 787 310 3864
Fragments arranged by size:
3864 971 787 310
BbvCI CC'TCA_GC
Cuts at: 2097 2891 2097
Size: 794 5138
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4123 4157 1903
Size: 34 2186 34 3678
Fragments arranged by size:
3678 2186 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3824 5034 3824
Size: 1210 4722
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5932
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4543 136
Size: 107 122 71 631 605 2871 1525
Fragments arranged by size:
2871 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5927 5927
Size: 5932
BlpI GC'TnA_GC
Cuts at: 3349 3349
Size: 5932
Bme1580I G_kGCm'C
Cuts at: 1790 3191 3993 5149 1790
Size: 1401 802 1156 2573
Fragments arranged by size:
2573 1401 1156 802
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5932
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3069 3663 4621
Size: 416 53 83 399 24 1652 594 958
Cuts at: 4621 442
Size: 1753
Fragments arranged by size:
1753 1652 958 594 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5932
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2105 4592 1239
Size: 866 2487 2579
Fragments arranged by size:
2579 2487 866
Bpu10I CC'TnA_GC
Cuts at: 2097 2129 2891 2097
Size: 32 762 5138
Fragments arranged by size:
5138 762 32
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2159 4057 4922 5220 2159
Size: 1898 865 298 2871
Fragments arranged by size:
2871 1898 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2932 4595 882
Size: 617 1433 1663 2219
Fragments arranged by size:
2219 1663 1433 617
BsaAI yAC'GTr
Cuts at: 493 1204 3723 493
Size: 711 2519 2702
Fragments arranged by size:
2702 2519 711
BsaBI GATnn'nnATC
Cuts at: 2816 5926 2816
Size: 3110 2822
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2164 3739 4121 4756
Size: 53 83 186 936 631 1575 382 635
Cuts at: 4756 275
Size: 1451
Fragments arranged by size:
1575 1451 936 635 631 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3159 4365 4763 5037 5184 5595 3159
Size: 1206 398 274 147 411 3496
Fragments arranged by size:
3496 1206 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3212 3242 1178
Size: 30 165 30 1809 30 3868
Fragments arranged by size:
3868 1809 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2024 2483 2704 2848 3233 1186
Size: 838 459 221 144 385 3885
Fragments arranged by size:
3885 838 459 385 221 144
BseYI C'CCAG_C
Cuts at: 2801 3021 5135 5631 2801
Size: 220 2114 496 3102
Fragments arranged by size:
3102 2114 496 220
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2120 1163
Size: 573 384 4975
Fragments arranged by size:
4975 573 384
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3382 4143 5171 1540
Size: 275 186 1381 761 1028 2301
Fragments arranged by size:
2301 1381 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2913 3993 4078 5149 2913
Size: 1080 85 1071 3696
Fragments arranged by size:
3696 1080 1071 85
BsmI GAATG_Cn'
Cuts at: 3443 3536 3443
Size: 93 5839
BsmBI CGTCTCn'nnnn_
Cuts at: 2246 3127 2246
Size: 881 5051
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2441 2801 2913 2977 3138 3191 3251
Size: 399 651 360 112 64 161 53 60
Cuts at: 3251 3993 4078 5149 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 651 399 360 161 112
85 64 60 53
BspEI T'CCGG_A
Cuts at: 5595 5595
Size: 5932
BspHI T'CATG_A
Cuts at: 2728 3821 5551 2728
Size: 1093 1730 3109
Fragments arranged by size:
3109 1730 1093
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3287 844
Size: 2443 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3819 1394
Size: 429 1996 3507
Fragments arranged by size:
3507 1996 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4427 4601 65
Size: 1183 3179 174 1396
Fragments arranged by size:
3179 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2577 4576 4763 1700
Size: 110 767 1999 187 2869
Fragments arranged by size:
2869 1999 767 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5932
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5932
BssSI C'ACGA_G
Cuts at: 2548 3992 5004 2548
Size: 1444 1012 3476
Fragments arranged by size:
3476 1444 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5932
BstBI TT'CG_AA
Cuts at: 4736 4736
Size: 5932
BstEII G'GTnAC_C
Cuts at: 1184 2107 1184
Size: 923 5009
BstYI r'GATC_y
Cuts at: 1972 3262 3629 4014 4031 5483 5569 5581 5927
Size: 1290 367 385 17 1452 86 12 346
Cuts at: 5927 1972
Size: 1977
Fragments arranged by size:
1977 1452 1290 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3191 513
Size: 552 939 1187 3254
Fragments arranged by size:
3254 1187 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5193
Fragments arranged by size:
5193 683 56
BtsI GCAGTG_nn'
Cuts at: 2035 2044 2835 3448 4242 4262 2035
Size: 9 791 613 794 20 3705
Fragments arranged by size:
3705 794 791 613 20 9
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5932
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5897
DraI TTT'AAA
Cuts at: 3236 3236
Size: 5932
DraIII CAC_nnn'GTG
Cuts at: 2305 2305
Size: 5932
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4939 809
Size: 507 3623 1802
Fragments arranged by size:
3623 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3379 4268 5897
Size: 54 1425 50 275 18 1549 889 1629
Cuts at: 5897 8
Size: 43
Fragments arranged by size:
1629 1549 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3379 1537
Size: 275 1567 4090
Fragments arranged by size:
4090 1567 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2348 2930 3862 1353
Size: 995 582 932 3423
Fragments arranged by size:
3423 995 932 582
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2686 4519 4893 5039 387
Size: 1064 138 1097 1833 374 146 1280
Fragments arranged by size:
1833 1280 1097 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2105 2857 2954 3995 4592 5379 5647
Size: 393 473 752 97 1041 597 787 268
Cuts at: 5647 1239
Size: 1524
Fragments arranged by size:
1524 1041 787 752 597 473 393 268
97
EcoRI G'AATT_C
Cuts at: 2324 2510 3241 3619 2324
Size: 186 731 378 4637
Fragments arranged by size:
4637 731 378 186
FspI TGC'GCA
Cuts at: 4438 4438
Size: 5932
HaeII r_GCGC'y
Cuts at: 1678 5079 1678
Size: 3401 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4573 4605 4647 4679 1373
Size: 32 135 32 3001 32 42 32 2626
Fragments arranged by size:
3001 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3130 3276 3522 669
Size: 988 281 1192 146 246 3079
Fragments arranged by size:
3079 1192 988 281 246 146
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5932
HpaI GTT'AAC
Cuts at: 3522 3522
Size: 5932
KpnI G_GTAC'C
Cuts at: 3257 3257
Size: 5932
MfeI C'AATT_G
Cuts at: 3531 3531
Size: 5932
MluI A'CGCG_T
Cuts at: 5808 5808
Size: 5932
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5046 5230 1305
Size: 3741 184 2007
Fragments arranged by size:
3741 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4110
Fragments arranged by size:
4110 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2307 2498 3890 4249 4408 518
Size: 648 1141 191 1392 359 159 2042
Fragments arranged by size:
2042 1392 1141 648 359 191 159
MspA1I CmG'CkG
Cuts at: 1606 2055 2853 2865 3348 4025 5173 5418 1606
Size: 449 798 12 483 677 1148 245 2120
Fragments arranged by size:
2120 1148 798 677 483 449 245 12
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4441
Fragments arranged by size:
4441 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5932
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5932
NotI GC'GGCC_GC
Cuts at: 3379 3379
Size: 5932
NspI r_CATG'y
Cuts at: 1132 1229 2609 3152 3290 4835 1132
Size: 97 1380 543 138 1545 2229
Fragments arranged by size:
2229 1545 1380 543 138 97
PasI CC'CwG_GG
Cuts at: 1348 2463 1348
Size: 1115 4817
PciI A'CATG_T
Cuts at: 4831 4831
Size: 5932
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5932
PfoI T'CCnGG_A
Cuts at: 2514 2514
Size: 5932
PmeI GTTT'AAAC
Cuts at: 3236 3236
Size: 5932
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4001 4033 5514 5546 4001
Size: 32 1481 32 4387
Fragments arranged by size:
4387 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3200 1613
Size: 1587 4345
PsiI TTA'TAA
Cuts at: 3502 3502
Size: 5932
PspOMI G'GGCC_C
Cuts at: 3187 3187
Size: 5932
PstI C_TGCA'G
Cuts at: 830 2460 2830 3088 3284 830
Size: 1630 370 258 196 3478
Fragments arranged by size:
3478 1630 370 258 196
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5932
PvuII CAG'CTG
Cuts at: 2853 2853
Size: 5932
RsrII CG'GwC_CG
Cuts at: 3162 3162
Size: 5932
SalI G'TCGA_C
Cuts at: 1936 3274 1936
Size: 1338 4594
SbfI CC_TGCA'GG
Cuts at: 3284 3284
Size: 5932
ScaI AGT'ACT
Cuts at: 4180 5892 4180
Size: 1712 4220
SfcI C'TryA_G
Cuts at: 826 1045 2456 2826 3066 3084 3280 4415 5096
Size: 219 1411 370 240 18 196 1135 681
Cuts at: 5096 5287 826
Size: 191 1471
Fragments arranged by size:
1471 1411 1135 681 370 240 219 196
191 18
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5932
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5932
SmaI CCC'GGG
Cuts at: 1872 2971 3259 1872
Size: 1099 288 4545
Fragments arranged by size:
4545 1099 288
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2174 4036 4937 5199 820
Size: 197 926 231 1862 901 262 1553
Fragments arranged by size:
1862 1553 926 901 262 231 197
SnaBI TAC'GTA
Cuts at: 493 3723 493
Size: 3230 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5932
SphI G_CATG'C
Cuts at: 1132 3290 1132
Size: 2158 3774
SspI AAT'ATT
Cuts at: 5 52 3856 5
Size: 47 3804 2081
Fragments arranged by size:
3804 2081 47
StuI AGG'CCT
Cuts at: 1517 2399 1517
Size: 882 5050
StyI C'CwwG_G
Cuts at: 513 1065 2004 3371 513
Size: 552 939 1367 3074
Fragments arranged by size:
3074 1367 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2637 3959 3976 4129 4314 4759 325
Size: 1255 1057 1322 17 153 185 445 1498
Fragments arranged by size:
1498 1322 1255 1057 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4178 5890 96
Size: 274 80 33 51 1443 2201 1712 138
Fragments arranged by size:
2201 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3212 3244 5892 5924 3212
Size: 32 2648 32 3220
Fragments arranged by size:
3220 2648 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5932
XbaI T'CTAG_A
Cuts at: 3268 3268
Size: 5932
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5932
XmaI C'CCGG_G
Cuts at: 1870 2969 3257 1870
Size: 1099 288 4545
Fragments arranged by size:
4545 1099 288
XmnI GAAnn'nnTTC
Cuts at: 2993 4061 2993
Size: 1068 4864
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2165 3740 276
Size: 53 83 186 936 631 1575 2468
Fragments arranged by size:
2468 1575 936 631 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII RsrII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspXI PsrI SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI