Restriction map for
pFN21AB8662
(Circular) (Six-base) MAPSORT of: pFN21AB8662.seq Check: 6458 from: 1 to: 5836
pFN21AB8662 5836 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3646 278
Size: 53 83 186 936 2110 2468
Fragments arranged by size:
2468 2110 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3179 1937
Size: 1242 4594
Acc65I G'GTAC_C
Cuts at: 3157 3157
Size: 5836
AclI AA'CG_TT
Cuts at: 1563 3963 4336 1563
Size: 2400 373 3063
Fragments arranged by size:
3063 2400 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3899 5283 5551 1632
Size: 2267 1384 268 1917
Fragments arranged by size:
2267 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5639
AflIII A'CryG_T
Cuts at: 1203 4735 5712 1203
Size: 3532 977 1327
Fragments arranged by size:
3532 1327 977
AgeI A'CCGG_T
Cuts at: 4667 4667
Size: 5836
AhdI GACnn_n'nnGTC
Cuts at: 4565 4565
Size: 5836
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5836
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5836
AlwNI CAG_nnn'CTG
Cuts at: 3579 5151 3579
Size: 1572 4264
ApaLI G'TGCA_C
Cuts at: 3893 5049 3893
Size: 1156 4680
ApoI r'AATT_y
Cuts at: 1493 2415 3145 3371 3523 5632 1493
Size: 922 730 226 152 2109 1697
Fragments arranged by size:
2109 1697 922 730 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4390 160
Size: 872 3358 1606
Fragments arranged by size:
3358 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5836
AvaI C'yCGr_G
Cuts at: 1870 1943 3161 1870
Size: 73 1218 4545
Fragments arranged by size:
4545 1218 73
BamHI G'GATC_C
Cuts at: 3166 3533 3166
Size: 367 5469
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2956 3157 4612 618
Size: 325 217 627 1169 201 1455 1842
Fragments arranged by size:
1842 1455 1169 627 325 217 201
BanII G_rGCy'C
Cuts at: 1391 2103 2172 3155 1391
Size: 712 69 983 4072
Fragments arranged by size:
4072 983 712 69
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4865
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2425 2459 4027 4061 1903
Size: 34 488 34 1568 34 3678
Fragments arranged by size:
3678 1568 488 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3728 4938 3728
Size: 1210 4626
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5836
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4447 136
Size: 107 122 71 631 605 2775 1525
Fragments arranged by size:
2775 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2835 2988 5831 2835
Size: 153 2843 2840
Fragments arranged by size:
2843 2840 153
BlpI GC'TnA_GC
Cuts at: 2102 3253 2102
Size: 1151 4685
Bme1580I G_kGCm'C
Cuts at: 1790 2959 3897 5053 1790
Size: 1169 938 1156 2573
Fragments arranged by size:
2573 1169 1156 938
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5836
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3567 4525 442
Size: 416 53 83 399 24 2150 958 1753
Fragments arranged by size:
2150 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5836
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2081 2186 3026 4496 1239
Size: 842 105 840 1470 2579
Fragments arranged by size:
2579 1470 842 840 105
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2327 2723 3961 4826 5124 2327
Size: 396 1238 865 298 3039
Fragments arranged by size:
3039 1238 865 396 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4499 882
Size: 617 3000 2219
Fragments arranged by size:
3000 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3627 493
Size: 711 2423 2702
Fragments arranged by size:
2702 2423 711
BsaBI GATnn'nnATC
Cuts at: 2254 5830 2254
Size: 3576 2260
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3643 4025 4660 275
Size: 53 83 186 936 2110 382 635 1451
Fragments arranged by size:
2110 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2931 4269 4667 4941 5088 5499 2931
Size: 1338 398 274 147 411 3268
Fragments arranged by size:
3268 1338 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5611
Fragments arranged by size:
5611 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2111 2420 2906 1186
Size: 925 309 486 4116
Fragments arranged by size:
4116 925 486 309
BseYI C'CCAG_C
Cuts at: 2736 3128 5039 5535 2736
Size: 392 1911 496 3037
Fragments arranged by size:
3037 1911 496 392
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5263
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3286 4047 5075 1540
Size: 275 186 1285 761 1028 2301
Fragments arranged by size:
2301 1285 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2103 3897 3982 5053 2103
Size: 1794 85 1071 2886
Fragments arranged by size:
2886 1794 1071 85
BsmI GAATG_Cn'
Cuts at: 3347 3440 3347
Size: 93 5743
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2103 2172 2959 3155 3897 3982 5053
Size: 399 313 69 787 196 742 85 1071
Cuts at: 5053 1391
Size: 2174
Fragments arranged by size:
2174 1071 787 742 399 313 196 85
69
BspEI T'CCGG_A
Cuts at: 2931 5499 2931
Size: 2568 3268
BspHI T'CATG_A
Cuts at: 3725 5455 3725
Size: 1730 4106
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2388 2758 3191 844
Size: 1544 370 433 3489
Fragments arranged by size:
3489 1544 433 370
BsrBI CCG'CTC
Cuts at: 1394 1823 3723 1394
Size: 429 1900 3507
Fragments arranged by size:
3507 1900 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2811 4331 4505 65
Size: 1183 1563 1520 174 1396
Fragments arranged by size:
1563 1520 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4480 4667 1700
Size: 110 2670 187 2869
Fragments arranged by size:
2869 2670 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5836
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5836
BssSI C'ACGA_G
Cuts at: 2390 3896 4908 2390
Size: 1506 1012 3318
Fragments arranged by size:
3318 1506 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2225 794
Size: 1431 4405
BstBI TT'CG_AA
Cuts at: 4640 4640
Size: 5836
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5836
BstYI r'GATC_y
Cuts at: 1972 2249 2835 2988 3166 3533 3918 3935 5387
Size: 277 586 153 178 367 385 17 1452
Cuts at: 5387 5473 5485 5831 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 586 385 367 346 277 178
153 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2275 513
Size: 552 1210 4074
Fragments arranged by size:
4074 1210 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5097
Fragments arranged by size:
5097 683 56
BtsI GCAGTG_nn'
Cuts at: 2922 3352 4146 4166 2922
Size: 430 794 20 4592
Fragments arranged by size:
4592 794 430 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5836
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5801
DraI TTT'AAA
Cuts at: 3140 3140
Size: 5836
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4843 809
Size: 507 3527 1802
Fragments arranged by size:
3527 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2502 2943 3283
Size: 54 1425 50 275 18 672 441 340
Cuts at: 3283 4172 5801 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 672 441 340 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3283 1537
Size: 275 1471 4090
Fragments arranged by size:
4090 1471 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2631 3005 3766 1353
Size: 1278 374 761 3423
Fragments arranged by size:
3423 1278 761 374
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4423 4797 4943 387
Size: 1064 138 2834 374 146 1280
Fragments arranged by size:
2834 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2101 2101
Size: 5836
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2081 2186 3026 3899 4496 5283 5551
Size: 393 449 105 840 873 597 787 268
Cuts at: 5551 1239
Size: 1524
Fragments arranged by size:
1524 873 840 787 597 449 393 268
105
EcoO109I rG'GnC_Cy
Cuts at: 2650 2650
Size: 5836
EcoRI G'AATT_C
Cuts at: 3145 3523 3145
Size: 378 5458
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2362 2854 2873 2362
Size: 492 19 5325
Fragments arranged by size:
5325 492 19
FspI TGC'GCA
Cuts at: 4342 4342
Size: 5836
HaeII r_GCGC'y
Cuts at: 1678 4983 1678
Size: 3305 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2003 2035 4477 4509 4551
Size: 32 135 32 431 32 2442 32 42
Cuts at: 4551 4583 1373
Size: 32 2626
Fragments arranged by size:
2626 2442 431 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3180 3426 669
Size: 988 281 1242 246 3079
Fragments arranged by size:
3079 1242 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2792 748
Size: 2044 3792
HpaI GTT'AAC
Cuts at: 3426 3426
Size: 5836
KpnI G_GTAC'C
Cuts at: 3161 3161
Size: 5836
MfeI C'AATT_G
Cuts at: 2621 2709 3435 2621
Size: 88 726 5022
Fragments arranged by size:
5022 726 88
MluI A'CGCG_T
Cuts at: 5712 5712
Size: 5836
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2034 2061 4950 5134 1305
Size: 729 27 2889 184 2007
Fragments arranged by size:
2889 2007 729 184 27
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2504 10
Size: 54 1425 343 672 3342
Fragments arranged by size:
3342 1425 672 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3020 3794 4153 4312 518
Size: 648 1854 774 359 159 2042
Fragments arranged by size:
2042 1854 774 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2080 2941 3252 3929 5077 5322 1606
Size: 474 861 311 677 1148 245 2120
Fragments arranged by size:
2120 1148 861 677 474 311 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5284
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5836
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5836
NotI GC'GGCC_GC
Cuts at: 3283 3283
Size: 5836
NspI r_CATG'y
Cuts at: 1132 1229 3194 4739 1132
Size: 97 1965 1545 2229
Fragments arranged by size:
2229 1965 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2172 1348
Size: 824 5012
PciI A'CATG_T
Cuts at: 4735 4735
Size: 5836
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5836
PfoI T'CCnGG_A
Cuts at: 2258 2979 2258
Size: 721 5115
PmeI GTTT'AAAC
Cuts at: 3140 3140
Size: 5836
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3905 3937 5418 5450 3905
Size: 32 1481 32 4291
Fragments arranged by size:
4291 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2975 1613
Size: 1362 4474
PsiI TTA'TAA
Cuts at: 3406 3406
Size: 5836
PstI C_TGCA'G
Cuts at: 830 2385 2769 3188 830
Size: 1555 384 419 3478
Fragments arranged by size:
3478 1555 419 384
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5836
PvuII CAG'CTG
Cuts at: 2080 2941 2080
Size: 861 4975
SacI G_AGCT'C
Cuts at: 2103 2103
Size: 5836
SalI G'TCGA_C
Cuts at: 1936 3178 1936
Size: 1242 4594
SapI GCTCTTCn'nnn_
Cuts at: 3005 3005
Size: 5836
SbfI CC_TGCA'GG
Cuts at: 3188 3188
Size: 5836
ScaI AGT'ACT
Cuts at: 4084 5796 4084
Size: 1712 4124
SfcI C'TryA_G
Cuts at: 826 1045 2381 2765 3184 4319 5000 5191 826
Size: 219 1336 384 419 1135 681 191 1471
Fragments arranged by size:
1471 1336 1135 681 419 384 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5836
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5836
SmaI CCC'GGG
Cuts at: 1872 3163 1872
Size: 1291 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2306 2702 3940 4841 5103 820
Size: 197 926 363 396 1238 901 262 1553
Fragments arranged by size:
1553 1238 926 901 396 363 262 197
SnaBI TAC'GTA
Cuts at: 493 3627 493
Size: 3134 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5836
SphI G_CATG'C
Cuts at: 1132 3194 1132
Size: 2062 3774
SspI AAT'ATT
Cuts at: 5 52 2153 3760 5
Size: 47 2101 1607 2081
Fragments arranged by size:
2101 2081 1607 47
StuI AGG'CCT
Cuts at: 1517 2286 1517
Size: 769 5067
StyI C'CwwG_G
Cuts at: 513 1065 3275 513
Size: 552 2210 3074
Fragments arranged by size:
3074 2210 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3863 3880 4033 4218 4663 325
Size: 1255 2283 17 153 185 445 1498
Fragments arranged by size:
2283 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4082 5794 96
Size: 274 80 33 51 1443 2105 1712 138
Fragments arranged by size:
2105 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5796 5828 5796
Size: 32 5804
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5836
XbaI T'CTAG_A
Cuts at: 3172 3172
Size: 5836
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5836
XmaI C'CCGG_G
Cuts at: 1870 3161 1870
Size: 1291 4545
XmnI GAAnn'nnTTC
Cuts at: 3965 3965
Size: 5836
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3644 276
Size: 53 83 186 936 2110 2468
Fragments arranged by size:
2468 2110 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI
SrfI SwaI XcmI