Restriction map for
pFN21AB8653
(Circular) (Six-base) MAPSORT of: pFN21AB8653.seq Check: 7037 from: 1 to: 5407
pFN21AB8653 5407 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2255 2255
Size: 5407
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3217 278
Size: 53 83 186 936 1681 2468
Fragments arranged by size:
2468 1681 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2750 1937
Size: 813 4594
Acc65I G'GTAC_C
Cuts at: 2517 2728 2517
Size: 211 5196
AclI AA'CG_TT
Cuts at: 1563 3534 3907 1563
Size: 1971 373 3063
Fragments arranged by size:
3063 1971 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3470 4854 5122 1632
Size: 1838 1384 268 1917
Fragments arranged by size:
1917 1838 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5210
AflIII A'CryG_T
Cuts at: 1203 2271 4306 5283 1203
Size: 1068 2035 977 1327
Fragments arranged by size:
2035 1327 1068 977
AgeI A'CCGG_T
Cuts at: 4238 4238
Size: 5407
AhdI GACnn_n'nnGTC
Cuts at: 4136 4136
Size: 5407
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5407
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5407
AlwNI CAG_nnn'CTG
Cuts at: 3150 4722 3150
Size: 1572 3835
ApaLI G'TGCA_C
Cuts at: 3464 4620 3464
Size: 1156 4251
ApoI r'AATT_y
Cuts at: 1493 2716 2942 3094 5203 1493
Size: 1223 226 152 2109 1697
Fragments arranged by size:
2109 1697 1223 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3961 160
Size: 872 2929 1606
Fragments arranged by size:
2929 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5407
AvaI C'yCGr_G
Cuts at: 1870 1943 2445 2732 1870
Size: 73 502 287 4545
Fragments arranged by size:
4545 502 287 73
BamHI G'GATC_C
Cuts at: 2623 2737 3104 2623
Size: 114 367 4926
Fragments arranged by size:
4926 367 114
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2042 2517 2528 2642 2728
Size: 325 217 627 255 475 11 114 86
Cuts at: 2728 4183 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 475 325 255 217 114
86 11
BanII G_rGCy'C
Cuts at: 1391 2610 2682 2726 1391
Size: 1219 72 44 4072
Fragments arranged by size:
4072 1219 72 44
BbeI G_GCGC'C
Cuts at: 2046 2532 2046
Size: 486 4921
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4436
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3598 3632 1903
Size: 34 1661 34 3678
Fragments arranged by size:
3678 1661 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3299 4509 3299
Size: 1210 4197
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5407
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2348 2537 4018
Size: 107 122 71 631 605 676 189 1481
Cuts at: 4018 136
Size: 1525
Fragments arranged by size:
1525 1481 676 631 605 189 122 107
71
BglII A'GATC_T
Cuts at: 5402 5402
Size: 5407
BlpI GC'TnA_GC
Cuts at: 2824 2824
Size: 5407
Bme1580I G_kGCm'C
Cuts at: 1790 3468 4624 1790
Size: 1678 1156 2573
Fragments arranged by size:
2573 1678 1156
BmgBI CAC'GTC
Cuts at: 1318 2272 1318
Size: 954 4453
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2625 3138 4096
Size: 416 53 83 399 24 1208 513 958
Cuts at: 4096 442
Size: 1753
Fragments arranged by size:
1753 1208 958 513 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5407
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2563 4067 1239
Size: 1324 1504 2579
Fragments arranged by size:
2579 1504 1324
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3532 4397 4695 3532
Size: 865 298 4244
Fragments arranged by size:
4244 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2404 4070 882
Size: 617 905 1666 2219
Fragments arranged by size:
2219 1666 905 617
BsaAI yAC'GTr
Cuts at: 493 1204 2602 3198 493
Size: 711 1398 596 2702
Fragments arranged by size:
2702 1398 711 596
BsaBI GATnn'nnATC
Cuts at: 5401 5401
Size: 5407
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2043 2529 3214 3596
Size: 53 83 186 936 510 486 685 382
Cuts at: 3596 4231 275
Size: 635 1451
Fragments arranged by size:
1451 936 685 635 510 486 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3840 4238 4512 4659 5070 3840
Size: 398 274 147 411 4177
Fragments arranged by size:
4177 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2144 2174 1178
Size: 30 165 30 741 30 4411
Fragments arranged by size:
4411 741 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2186 1186
Size: 1000 4407
BseYI C'CCAG_C
Cuts at: 2567 4610 5106 2567
Size: 2043 496 2868
Fragments arranged by size:
2868 2043 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4834
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2857 3618 4646 1540
Size: 275 186 856 761 1028 2301
Fragments arranged by size:
2301 1028 856 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2235 3468 3553 4624 2235
Size: 1233 85 1071 3018
Fragments arranged by size:
3018 1233 1071 85
BsiWI C'GTAC_G
Cuts at: 2019 2019
Size: 5407
BsmI GAATG_Cn'
Cuts at: 2918 3011 2918
Size: 93 5314
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2235 2610 2682 2726 3468 3553 4624
Size: 399 445 375 72 44 742 85 1071
Cuts at: 4624 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 445 399 375 85 72
44
BspEI T'CCGG_A
Cuts at: 5070 5070
Size: 5407
BspHI T'CATG_A
Cuts at: 3296 5026 3296
Size: 1730 3677
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2255 2386 2762 844
Size: 1411 131 376 3489
Fragments arranged by size:
3489 1411 376 131
BsrBI CCG'CTC
Cuts at: 1394 1823 2027 3294 1394
Size: 429 204 1267 3507
Fragments arranged by size:
3507 1267 429 204
BsrDI GCAATG_nn'
Cuts at: 65 1248 3902 4076 65
Size: 1183 2654 174 1396
Fragments arranged by size:
2654 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2630 2656 4051 4238 1700
Size: 110 820 26 1395 187 2869
Fragments arranged by size:
2869 1395 820 187 110 26
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5407
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5407
BssSI C'ACGA_G
Cuts at: 3467 4479 3467
Size: 1012 4395
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5407
BstBI TT'CG_AA
Cuts at: 4211 4211
Size: 5407
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5407
BstYI r'GATC_y
Cuts at: 1972 2623 2737 3104 3489 3506 4958 5044 5056
Size: 651 114 367 385 17 1452 86 12
Cuts at: 5056 5402 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 651 385 367 346 114 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2223 513
Size: 552 939 219 3697
Fragments arranged by size:
3697 939 552 219
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2440 487
Size: 683 56 1214 3454
Fragments arranged by size:
3454 1214 683 56
BtsI GCAGTG_nn'
Cuts at: 2923 3717 3737 2923
Size: 794 20 4593
Fragments arranged by size:
4593 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5407
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5372
DraI TTT'AAA
Cuts at: 2711 2711
Size: 5407
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2333 4414 809
Size: 507 1017 2081 1802
Fragments arranged by size:
2081 1802 1017 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2220 2382 2658
Size: 54 1425 50 275 18 390 162 276
Cuts at: 2658 2854 3743 5372 8
Size: 196 889 1629 43
Fragments arranged by size:
1629 1425 889 390 276 275 196 162
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2854 1537
Size: 275 1042 4090
Fragments arranged by size:
4090 1042 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2549 3337 1353
Size: 1196 788 3423
Fragments arranged by size:
3423 1196 788
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3994 4368 4514 387
Size: 1064 138 2405 374 146 1280
Fragments arranged by size:
2405 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2563 3470 4067 4854 5122 1239
Size: 393 931 907 597 787 268 1524
Fragments arranged by size:
1524 931 907 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2716 3094 2716
Size: 378 5029
FspI TGC'GCA
Cuts at: 3913 3913
Size: 5407
HaeII r_GCGC'y
Cuts at: 1678 2046 2160 2532 4554 1678
Size: 368 114 372 2022 2531
Fragments arranged by size:
2531 2022 372 368 114
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4048 4080 4122 4154 1373
Size: 32 135 32 2476 32 42 32 2626
Fragments arranged by size:
2626 2476 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2751 2997 669
Size: 988 281 813 246 3079
Fragments arranged by size:
3079 988 813 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5407
HpaI GTT'AAC
Cuts at: 2997 2997
Size: 5407
KasI G'GCGC_C
Cuts at: 2042 2528 2042
Size: 486 4921
KpnI G_GTAC'C
Cuts at: 2521 2732 2521
Size: 211 5196
MfeI C'AATT_G
Cuts at: 3006 3006
Size: 5407
MluI A'CGCG_T
Cuts at: 5283 5283
Size: 5407
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2349 4521 4705 1305
Size: 1044 2172 184 2007
Fragments arranged by size:
2172 2007 1044 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2384 10
Size: 54 1425 343 552 3033
Fragments arranged by size:
3033 1425 552 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2117 3365 3724 3883 518
Size: 648 951 1248 359 159 2042
Fragments arranged by size:
2042 1248 951 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2515 2823 3500 4648 4893 1606
Size: 909 308 677 1148 245 2120
Fragments arranged by size:
2120 1148 909 677 308 245
NaeI GCC'GGC
Cuts at: 2658 2658
Size: 5407
NarI GG'CG_CC
Cuts at: 2043 2529 2043
Size: 486 4921
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3916
Fragments arranged by size:
3916 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5407
NgoMIV G'CCGG_C
Cuts at: 2656 2656
Size: 5407
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5407
NotI GC'GGCC_GC
Cuts at: 2854 2854
Size: 5407
NspI r_CATG'y
Cuts at: 1132 1229 2587 2765 4310 1132
Size: 97 1358 178 1545 2229
Fragments arranged by size:
2229 1545 1358 178 97
PasI CC'CwG_GG
Cuts at: 1348 2683 1348
Size: 1335 4072
PciI A'CATG_T
Cuts at: 4306 4306
Size: 5407
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2391 1108
Size: 1283 4124
PmeI GTTT'AAAC
Cuts at: 2711 2711
Size: 5407
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3476 3508 4989 5021 3476
Size: 32 1481 32 3862
Fragments arranged by size:
3862 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5407
PsiI TTA'TAA
Cuts at: 2977 2977
Size: 5407
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2129 2161 2129
Size: 32 5375
PstI C_TGCA'G
Cuts at: 830 2210 2759 830
Size: 1380 549 3478
Fragments arranged by size:
3478 1380 549
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5407
SalI G'TCGA_C
Cuts at: 1936 2749 1936
Size: 813 4594
SbfI CC_TGCA'GG
Cuts at: 2759 2759
Size: 5407
ScaI AGT'ACT
Cuts at: 3655 5367 3655
Size: 1712 3695
SfcI C'TryA_G
Cuts at: 826 1045 2206 2755 3890 4571 4762 826
Size: 219 1161 549 1135 681 191 1471
Fragments arranged by size:
1471 1161 1135 681 549 219 191
SfoI GGC'GCC
Cuts at: 2044 2530 2044
Size: 486 4921
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5407
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5407
SmaI CCC'GGG
Cuts at: 1872 2447 2734 1872
Size: 575 287 4545
Fragments arranged by size:
4545 575 287
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3511 4412 4674 820
Size: 197 926 1568 901 262 1553
Fragments arranged by size:
1568 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3198 493
Size: 2705 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5407
SphI G_CATG'C
Cuts at: 1132 2587 2765 1132
Size: 1455 178 3774
Fragments arranged by size:
3774 1455 178
SrfI GCCC'GGGC
Cuts at: 2447 2447
Size: 5407
SspI AAT'ATT
Cuts at: 5 52 3331 5
Size: 47 3279 2081
Fragments arranged by size:
3279 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5407
StyI C'CwwG_G
Cuts at: 513 1065 2004 2409 2846 513
Size: 552 939 405 437 3074
Fragments arranged by size:
3074 939 552 437 405
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3434 3451 3604 3789 4234 325
Size: 1255 1854 17 153 185 445 1498
Fragments arranged by size:
1854 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2274 3653 5365
Size: 274 80 33 51 1443 297 1379 1712
Cuts at: 5365 96
Size: 138
Fragments arranged by size:
1712 1443 1379 297 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5367 5399 5367
Size: 32 5375
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5407
XbaI T'CTAG_A
Cuts at: 2743 2743
Size: 5407
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5407
XmaI C'CCGG_G
Cuts at: 1870 2445 2732 1870
Size: 575 287 4545
Fragments arranged by size:
4545 575 287
XmnI GAAnn'nnTTC
Cuts at: 3536 3536
Size: 5407
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3215 276
Size: 53 83 186 936 1681 2468
Fragments arranged by size:
2468 1681 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PsrI PstI PvuI SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SwaI XcmI