Restriction map for
pFN21AB8651
(Circular) (Six-base) MAPSORT of: pFN21AB8651.seq Check: 2438 from: 1 to: 6163
pFN21AB8651 6163 bp
With 182 enzymes: SgfI *
August 16, 2010 14:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2677 2677
Size: 6163
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3973 278
Size: 53 83 186 936 2437 2468
Fragments arranged by size:
2468 2437 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3506 1937
Size: 1569 4594
Acc65I G'GTAC_C
Cuts at: 3484 3484
Size: 6163
AclI AA'CG_TT
Cuts at: 1563 4290 4663 1563
Size: 2727 373 3063
Fragments arranged by size:
3063 2727 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2660 4226 5610 5878 1632
Size: 1028 1566 1384 268 1917
Fragments arranged by size:
1917 1566 1384 1028 268
AflII C'TTAA_G
Cuts at: 820 1017 2609 820
Size: 197 1592 4374
Fragments arranged by size:
4374 1592 197
AflIII A'CryG_T
Cuts at: 1203 5062 6039 1203
Size: 3859 977 1327
Fragments arranged by size:
3859 1327 977
AgeI A'CCGG_T
Cuts at: 4994 4994
Size: 6163
AhdI GACnn_n'nnGTC
Cuts at: 3208 4892 3208
Size: 1684 4479
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6163
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6163
AlwNI CAG_nnn'CTG
Cuts at: 2058 3013 3906 5478 2058
Size: 955 893 1572 2743
Fragments arranged by size:
2743 1572 955 893
ApaI G_GGCC'C
Cuts at: 2194 2226 3304 2194
Size: 32 1078 5053
Fragments arranged by size:
5053 1078 32
ApaLI G'TGCA_C
Cuts at: 4220 5376 4220
Size: 1156 5007
ApoI r'AATT_y
Cuts at: 1493 3472 3698 3850 5959 1493
Size: 1979 226 152 2109 1697
Fragments arranged by size:
2109 1979 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4717 160
Size: 872 3685 1606
Fragments arranged by size:
3685 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6163
AvaI C'yCGr_G
Cuts at: 1870 1943 2297 3046 3277 3488 1870
Size: 73 354 749 231 211 4545
Fragments arranged by size:
4545 749 354 231 211 73
BamHI G'GATC_C
Cuts at: 3493 3860 3493
Size: 367 5796
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3484 4939 618
Size: 325 217 627 1697 1455 1842
Fragments arranged by size:
1842 1697 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2194 2226 3304 3482 1391
Size: 803 32 1078 178 4072
Fragments arranged by size:
4072 1078 803 178 32
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3179 928
Size: 971 1280 3912
Fragments arranged by size:
3912 1280 971
BbvCI CC'TCA_GC
Cuts at: 2406 2939 2406
Size: 533 5630
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4354 4388 1903
Size: 34 2417 34 3678
Fragments arranged by size:
3678 2417 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4055 5265 4055
Size: 1210 4953
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6163
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2255 4774 136
Size: 107 122 71 631 605 583 2519 1525
Fragments arranged by size:
2519 1525 631 605 583 122 107 71
BglII A'GATC_T
Cuts at: 6158 6158
Size: 6163
BlpI GC'TnA_GC
Cuts at: 3338 3580 3338
Size: 242 5921
Bme1580I G_kGCm'C
Cuts at: 1790 2194 2226 2660 3304 4224 5380 1790
Size: 404 32 434 644 920 1156 2573
Fragments arranged by size:
2573 1156 920 644 434 404 32
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6163
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2221 2659 3055
Size: 416 53 83 399 24 804 438 396
Cuts at: 3055 3894 4852 442
Size: 839 958 1753
Fragments arranged by size:
1753 958 839 804 438 416 399 396
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6163
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2221 2771 2834 4823 1239
Size: 982 550 63 1989 2579
Fragments arranged by size:
2579 1989 982 550 63
Bpu10I CC'TnA_GC
Cuts at: 2406 2939 2406
Size: 533 5630
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2549 4288 5153 5451 2549
Size: 1739 865 298 3261
Fragments arranged by size:
3261 1739 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2387 4826 882
Size: 617 888 2439 2219
Fragments arranged by size:
2439 2219 888 617
BsaAI yAC'GTr
Cuts at: 493 1204 3954 493
Size: 711 2750 2702
Fragments arranged by size:
2750 2702 711
BsaBI GATnn'nnATC
Cuts at: 6157 6157
Size: 6163
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2973 3270 3970 4352
Size: 53 83 186 936 1440 297 700 382
Cuts at: 4352 4987 275
Size: 635 1451
Fragments arranged by size:
1451 1440 936 700 635 382 297 186
83 53
BsaWI w'CCGG_w
Cuts at: 4596 4994 5268 5415 5826 4596
Size: 398 274 147 411 4933
Fragments arranged by size:
4933 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2025 2055 2675 2705 3209
Size: 30 165 30 622 30 620 30 504
Cuts at: 3209 3239 1178
Size: 30 4102
Fragments arranged by size:
4102 622 620 504 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2513 2729 2924 3217 1186
Size: 1327 216 195 293 4132
Fragments arranged by size:
4132 1327 293 216 195
BseYI C'CCAG_C
Cuts at: 3026 3350 5366 5862 3026
Size: 324 2016 496 3327
Fragments arranged by size:
3327 2016 496 324
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2137 2276 1163
Size: 573 401 139 5050
Fragments arranged by size:
5050 573 401 139
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3613 4374 5402 1540
Size: 275 186 1612 761 1028 2301
Fragments arranged by size:
2301 1612 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4224 4309 5380 4224
Size: 85 1071 5007
Fragments arranged by size:
5007 1071 85
BsmI GAATG_Cn'
Cuts at: 3674 3767 3674
Size: 93 6070
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2194 2226 2660 3304 3482 4224 4309
Size: 399 404 32 434 644 178 742 85
Cuts at: 4309 5380 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 644 434 404 399 178
85 32
BspEI T'CCGG_A
Cuts at: 5826 5826
Size: 6163
BspHI T'CATG_A
Cuts at: 2127 4052 5782 2127
Size: 1925 1730 2508
Fragments arranged by size:
2508 1925 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2677 3518 844
Size: 1833 841 3489
Fragments arranged by size:
3489 1833 841
BsrBI CCG'CTC
Cuts at: 1394 1823 2146 4050 1394
Size: 429 323 1904 3507
Fragments arranged by size:
3507 1904 429 323
BsrDI GCAATG_nn'
Cuts at: 65 1248 4658 4832 65
Size: 1183 3410 174 1396
Fragments arranged by size:
3410 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4807 4994 1700
Size: 110 2997 187 2869
Fragments arranged by size:
2997 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6163
BssHII G'CGCG_C
Cuts at: 1925 2150 1925
Size: 225 5938
BssSI C'ACGA_G
Cuts at: 2210 2517 4223 5235 2210
Size: 307 1706 1012 3138
Fragments arranged by size:
3138 1706 1012 307
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2837 794
Size: 2043 4120
BstBI TT'CG_AA
Cuts at: 4967 4967
Size: 6163
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6163
BstYI r'GATC_y
Cuts at: 1972 3493 3860 4245 4262 5714 5800 5812 6158
Size: 1521 367 385 17 1452 86 12 346
Cuts at: 6158 1972
Size: 1977
Fragments arranged by size:
1977 1521 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2852 2852
Size: 6163
BtgI C'CryG_G
Cuts at: 513 1065 2238 3231 513
Size: 552 1173 993 3445
Fragments arranged by size:
3445 1173 993 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5424
Fragments arranged by size:
5424 683 56
BtsI GCAGTG_nn'
Cuts at: 2705 2847 3679 4473 4493 2705
Size: 142 832 794 20 4375
Fragments arranged by size:
4375 832 794 142 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6163
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3429 3464 529
Size: 35 2865 35 3228
Fragments arranged by size:
3228 2865 35 35
DraI TTT'AAA
Cuts at: 3467 3467
Size: 6163
DraIII CAC_nnn'GTG
Cuts at: 2187 2187
Size: 6163
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5170 809
Size: 507 3854 1802
Fragments arranged by size:
3854 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2104 2886 2897
Size: 54 1425 50 275 18 274 782 11
Cuts at: 2897 3610 4499 6128 8
Size: 713 889 1629 43
Fragments arranged by size:
1629 1425 889 782 713 275 274 54
50 43 18 11
EagI C'GGCC_G
Cuts at: 1537 1812 3610 1537
Size: 275 1798 4090
Fragments arranged by size:
4090 1798 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4093 1353
Size: 2740 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4750 5124 5270 387
Size: 1064 138 3161 374 146 1280
Fragments arranged by size:
3161 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2221 2660 2771 2834 4226 4823 5610
Size: 393 589 439 111 63 1392 597 787
Cuts at: 5610 5878 1239
Size: 268 1524
Fragments arranged by size:
1524 1392 787 597 589 439 393 268
111 63
EcoO109I rG'GnC_Cy
Cuts at: 2222 2223 2848 3301 2222
Size: 1 625 453 5084
Fragments arranged by size:
5084 625 453 1
EcoRI G'AATT_C
Cuts at: 3472 3850 3472
Size: 378 5785
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2377 2624 3424 2377
Size: 247 800 5116
Fragments arranged by size:
5116 800 247
FspI TGC'GCA
Cuts at: 2172 3069 4669 2172
Size: 897 1600 3666
Fragments arranged by size:
3666 1600 897
HaeII r_GCGC'y
Cuts at: 1678 2200 5310 1678
Size: 522 3110 2531
Fragments arranged by size:
3110 2531 522
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2900 2932 3194 3226 3260
Size: 32 135 32 1328 32 262 32 34
Cuts at: 3260 3292 4804 4836 4878 4910 1373
Size: 32 1512 32 42 32 2626
Fragments arranged by size:
2626 1512 1328 262 135 42 34 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3507 3753 669
Size: 988 281 1569 246 3079
Fragments arranged by size:
3079 1569 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6163
HpaI GTT'AAC
Cuts at: 3753 3753
Size: 6163
KpnI G_GTAC'C
Cuts at: 3488 3488
Size: 6163
MfeI C'AATT_G
Cuts at: 3762 3762
Size: 6163
MluI A'CGCG_T
Cuts at: 6039 6039
Size: 6163
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2011 5277 5461 1305
Size: 706 3266 184 2007
Fragments arranged by size:
3266 2007 706 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2106 2888 10
Size: 54 1425 343 274 782 3285
Fragments arranged by size:
3285 1425 782 343 274 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2888 4121 4480 4639 518
Size: 648 1722 1233 359 159 2042
Fragments arranged by size:
2042 1722 1233 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2834 3579 4256 5404 5649 1606
Size: 1228 745 677 1148 245 2120
Fragments arranged by size:
2120 1228 1148 745 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5611
NdeI CA'TA_TG
Cuts at: 387 2017 387
Size: 1630 4533
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6163
NotI GC'GGCC_GC
Cuts at: 3610 3610
Size: 6163
NspI r_CATG'y
Cuts at: 1132 1229 3521 5066 1132
Size: 97 2292 1545 2229
Fragments arranged by size:
2292 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2436 1348
Size: 1088 5075
PciI A'CATG_T
Cuts at: 5062 5062
Size: 6163
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2671 2812 2895 3195 1108
Size: 1563 141 83 300 4076
Fragments arranged by size:
4076 1563 300 141 83
PfoI T'CCnGG_A
Cuts at: 2736 2736
Size: 6163
PmeI GTTT'AAAC
Cuts at: 3467 3467
Size: 6163
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4232 4264 5745 5777 4232
Size: 32 1481 32 4618
Fragments arranged by size:
4618 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2848 2848
Size: 6163
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6163
PsiI TTA'TAA
Cuts at: 3733 3733
Size: 6163
PspOMI G'GGCC_C
Cuts at: 2190 2222 3300 2190
Size: 32 1078 5053
Fragments arranged by size:
5053 1078 32
PspXI vC'TCGA_Gb
Cuts at: 2297 2297
Size: 6163
PstI C_TGCA'G
Cuts at: 830 3515 830
Size: 2685 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6163
PvuII CAG'CTG
Cuts at: 2834 2834
Size: 6163
SalI G'TCGA_C
Cuts at: 1936 3505 1936
Size: 1569 4594
SbfI CC_TGCA'GG
Cuts at: 3515 3515
Size: 6163
ScaI AGT'ACT
Cuts at: 4411 6123 4411
Size: 1712 4451
SfcI C'TryA_G
Cuts at: 826 1045 3511 4646 5327 5518 826
Size: 219 2466 1135 681 191 1471
Fragments arranged by size:
2466 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2255 2255
Size: 6163
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6163
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6163
SmaI CCC'GGG
Cuts at: 1872 3490 1872
Size: 1618 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2297 2564 2609 4267 5168 5430
Size: 197 926 354 267 45 1658 901 262
Cuts at: 5430 820
Size: 1553
Fragments arranged by size:
1658 1553 926 901 354 267 262 197
45
SnaBI TAC'GTA
Cuts at: 493 3954 493
Size: 3461 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6163
SphI G_CATG'C
Cuts at: 1132 3521 1132
Size: 2389 3774
SspI AAT'ATT
Cuts at: 5 52 4087 5
Size: 47 4035 2081
Fragments arranged by size:
4035 2081 47
StuI AGG'CCT
Cuts at: 1517 2249 2309 1517
Size: 732 60 5371
Fragments arranged by size:
5371 732 60
StyI C'CwwG_G
Cuts at: 513 1065 2397 3602 513
Size: 552 1332 1205 3074
Fragments arranged by size:
3074 1332 1205 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4190 4207 4360 4545 4990 325
Size: 1255 2610 17 153 185 445 1498
Fragments arranged by size:
2610 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2667 4409 6121
Size: 274 80 33 51 1443 690 1742 1712
Cuts at: 6121 96
Size: 138
Fragments arranged by size:
1742 1712 1443 690 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2025 2057 2840 2872 6123 6155 2025
Size: 32 783 32 3251 32 2033
Fragments arranged by size:
3251 2033 783 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2972 1316
Size: 1656 4507
XbaI T'CTAG_A
Cuts at: 3499 3499
Size: 6163
XhoI C'TCGA_G
Cuts at: 1943 2297 1943
Size: 354 5809
XmaI C'CCGG_G
Cuts at: 1870 3488 1870
Size: 1618 4545
XmnI GAAnn'nnTTC
Cuts at: 2361 3257 4292 2361
Size: 896 1035 4232
Fragments arranged by size:
4232 1035 896
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3971 276
Size: 53 83 186 936 2437 2468
Fragments arranged by size:
2468 2437 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PsrI RsrII SacI SacII SanDI SapI SexAI
SfoI SrfI SwaI XcmI