Restriction map for
pFN21AB8650
(Circular) (Six-base) MAPSORT of: pFN21AB8650.seq Check: 9541 from: 1 to: 5695
pFN21AB8650 5695 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3505 278
Size: 53 83 186 936 1969 2468
Fragments arranged by size:
2468 1969 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2391 3038 1937
Size: 454 647 4594
Fragments arranged by size:
4594 647 454
Acc65I G'GTAC_C
Cuts at: 3016 3016
Size: 5695
AclI AA'CG_TT
Cuts at: 1563 3822 4195 1563
Size: 2259 373 3063
Fragments arranged by size:
3063 2259 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2022 3758 5142 5410 1632
Size: 390 1736 1384 268 1917
Fragments arranged by size:
1917 1736 1384 390 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5498
AflIII A'CryG_T
Cuts at: 1203 4594 5571 1203
Size: 3391 977 1327
Fragments arranged by size:
3391 1327 977
AgeI A'CCGG_T
Cuts at: 4526 4526
Size: 5695
AhdI GACnn_n'nnGTC
Cuts at: 2491 4424 2491
Size: 1933 3762
AleI CACnn'nnGTG
Cuts at: 1166 2514 1166
Size: 1348 4347
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5695
AlwNI CAG_nnn'CTG
Cuts at: 2644 3438 5010 2644
Size: 794 1572 3329
Fragments arranged by size:
3329 1572 794
ApaI G_GGCC'C
Cuts at: 2833 2833
Size: 5695
ApaLI G'TGCA_C
Cuts at: 3752 4908 3752
Size: 1156 4539
ApoI r'AATT_y
Cuts at: 1493 2149 2407 3004 3230 3382 5491 1493
Size: 656 258 597 226 152 2109 1697
Fragments arranged by size:
2109 1697 656 597 258 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4249 160
Size: 872 3217 1606
Fragments arranged by size:
3217 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5695
AvaI C'yCGr_G
Cuts at: 1870 1943 2451 2832 2991 3020 1870
Size: 73 508 381 159 29 4545
Fragments arranged by size:
4545 508 381 159 73 29
BamHI G'GATC_C
Cuts at: 3025 3392 3025
Size: 367 5328
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2738 2980 3016 4471 618
Size: 325 217 627 951 242 36 1455 1842
Fragments arranged by size:
1842 1455 951 627 325 242 217 36
BanII G_rGCy'C
Cuts at: 1391 2267 2833 3014 1391
Size: 876 566 181 4072
Fragments arranged by size:
4072 876 566 181
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2463 928
Size: 971 564 4160
Fragments arranged by size:
4160 971 564
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3886 3920 1903
Size: 34 1949 34 3678
Fragments arranged by size:
3678 1949 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3587 4797 3587
Size: 1210 4485
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5695
BfrBI ATG'CAT
Cuts at: 2776 2776
Size: 5695
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2706 4306 136
Size: 107 122 71 631 605 1034 1600 1525
Fragments arranged by size:
1600 1525 1034 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2430 5690 2430
Size: 3260 2435
BlpI GC'TnA_GC
Cuts at: 3112 3112
Size: 5695
Bme1580I G_kGCm'C
Cuts at: 1790 2145 2833 3756 4912 1790
Size: 355 688 923 1156 2573
Fragments arranged by size:
2573 1156 923 688 355
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5695
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2789 3426 4384
Size: 416 53 83 399 24 1372 637 958
Cuts at: 4384 442
Size: 1753
Fragments arranged by size:
1753 1372 958 637 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5695
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2088 2401 2789 2879 4355 1239
Size: 849 313 388 90 1476 2579
Fragments arranged by size:
2579 1476 849 388 313 90
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3820 4685 4983 3820
Size: 865 298 4532
Fragments arranged by size:
4532 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4358 882
Size: 617 2859 2219
Fragments arranged by size:
2859 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2977 3486 493
Size: 711 1773 509 2702
Fragments arranged by size:
2702 1773 711 509
BsaBI GATnn'nnATC
Cuts at: 5689 5689
Size: 5695
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3502 3884 4519 275
Size: 53 83 186 936 1969 382 635 1451
Fragments arranged by size:
1969 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2487 2553 4128 4526 4800 4947 5358 2487
Size: 66 1575 398 274 147 411 2824
Fragments arranged by size:
2824 1575 411 398 274 147 66
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5470
Fragments arranged by size:
5470 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2114 2718 1186
Size: 928 604 4163
Fragments arranged by size:
4163 928 604
BseYI C'CCAG_C
Cuts at: 2629 2637 2872 2953 4898 5394 2629
Size: 8 235 81 1945 496 2930
Fragments arranged by size:
2930 1945 496 235 81 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2819 2927 1163
Size: 573 1083 108 3931
Fragments arranged by size:
3931 1083 573 108
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2938 3145 3906 4934 1540
Size: 275 186 937 207 761 1028 2301
Fragments arranged by size:
2301 1028 937 761 275 207 186
BsiHKAI G_wGCw'C
Cuts at: 2267 3756 3841 4912 2267
Size: 1489 85 1071 3050
Fragments arranged by size:
3050 1489 1071 85
BsmI GAATG_Cn'
Cuts at: 2767 3206 3299 2767
Size: 439 93 5163
Fragments arranged by size:
5163 439 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2145 2267 2833 3014 3756 3841 4912
Size: 399 355 122 566 181 742 85 1071
Cuts at: 4912 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 566 399 355 181 122
85
BspEI T'CCGG_A
Cuts at: 5358 5358
Size: 5695
BspHI T'CATG_A
Cuts at: 3584 5314 3584
Size: 1730 3965
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3050 844
Size: 2206 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3582 1394
Size: 429 1759 3507
Fragments arranged by size:
3507 1759 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4190 4364 65
Size: 1183 2942 174 1396
Fragments arranged by size:
2942 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2741 2933 4339 4526 1700
Size: 110 931 192 1406 187 2869
Fragments arranged by size:
2869 1406 931 192 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5695
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5695
BssSI C'ACGA_G
Cuts at: 3755 4767 3755
Size: 1012 4683
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5695
BstBI TT'CG_AA
Cuts at: 4499 4499
Size: 5695
BstEII G'GTnAC_C
Cuts at: 1184 2465 1184
Size: 1281 4414
BstXI CCAn_nnnn'nTGG
Cuts at: 2928 2977 2928
Size: 49 5646
BstYI r'GATC_y
Cuts at: 1972 2430 3025 3392 3777 3794 5246 5332 5344
Size: 458 595 367 385 17 1452 86 12
Cuts at: 5344 5690 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 595 458 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4204
Fragments arranged by size:
4204 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4956
Fragments arranged by size:
4956 683 56
BtsI GCAGTG_nn'
Cuts at: 2031 2758 3211 4005 4025 2031
Size: 727 453 794 20 3701
Fragments arranged by size:
3701 794 727 453 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5695
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2084 2119 529
Size: 35 1520 35 4105
Fragments arranged by size:
4105 1520 35 35
DraI TTT'AAA
Cuts at: 2999 2999
Size: 5695
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4702 809
Size: 507 3386 1802
Fragments arranged by size:
3386 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2935 3142 4031
Size: 54 1425 50 275 18 1105 207 889
Cuts at: 4031 5660 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1105 889 275 207 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2935 3142 1537
Size: 275 1123 207 4090
Fragments arranged by size:
4090 1123 275 207
EarI CTCTTCn'nnn_
Cuts at: 1353 2966 3625 1353
Size: 1613 659 3423
Fragments arranged by size:
3423 1613 659
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2129 4282 4656 4802 387
Size: 1064 138 540 2153 374 146 1280
Fragments arranged by size:
2153 1280 1064 540 374 146 138
EcoICRI GAG'CTC
Cuts at: 2265 2265
Size: 5695
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2022 2088 2401 2789 2879 3758 4355
Size: 393 390 66 313 388 90 879 597
Cuts at: 4355 5142 5410 1239
Size: 787 268 1524
Fragments arranged by size:
1524 879 787 597 393 390 388 313
268 90 66
EcoO109I rG'GnC_Cy
Cuts at: 2493 2854 2902 2493
Size: 361 48 5286
Fragments arranged by size:
5286 361 48
EcoRI G'AATT_C
Cuts at: 3004 3382 3004
Size: 378 5317
EcoRV GAT'ATC
Cuts at: 2669 2669
Size: 5695
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2449 2449
Size: 5695
FspI TGC'GCA
Cuts at: 4201 4201
Size: 5695
HaeII r_GCGC'y
Cuts at: 1678 2636 2840 4842 1678
Size: 958 204 2002 2531
Fragments arranged by size:
2531 2002 958 204
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2477 2509 2520 2552 4336
Size: 32 135 32 905 32 11 32 1784
Cuts at: 4336 4368 4410 4442 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1784 905 135 42 32 32 32
32 32 32 11
HincII GTy'rAC
Cuts at: 669 1657 1938 3039 3285 669
Size: 988 281 1101 246 3079
Fragments arranged by size:
3079 1101 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5695
HpaI GTT'AAC
Cuts at: 3285 3285
Size: 5695
KpnI G_GTAC'C
Cuts at: 3020 3020
Size: 5695
MfeI C'AATT_G
Cuts at: 3294 3294
Size: 5695
MluI A'CGCG_T
Cuts at: 5571 5571
Size: 5695
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2544 2563 2940 4809 4993 1305
Size: 1239 19 377 1869 184 2007
Fragments arranged by size:
2007 1869 1239 377 184 19
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3873
Fragments arranged by size:
3873 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2301 2514 2926 3653 4012 4171 518
Size: 648 1135 213 412 727 359 159 2042
Fragments arranged by size:
2042 1135 727 648 412 359 213 159
MspA1I CmG'CkG
Cuts at: 1606 2644 2886 3111 3788 4936 5181 1606
Size: 1038 242 225 677 1148 245 2120
Fragments arranged by size:
2120 1148 1038 677 245 242 225
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4204
Fragments arranged by size:
4204 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5695
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5695
NotI GC'GGCC_GC
Cuts at: 3142 3142
Size: 5695
NsiI A_TGCA'T
Cuts at: 2778 2778
Size: 5695
NspI r_CATG'y
Cuts at: 1132 1229 3053 4598 1132
Size: 97 1824 1545 2229
Fragments arranged by size:
2229 1824 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2270 2497 1348
Size: 922 227 4546
Fragments arranged by size:
4546 922 227
PciI A'CATG_T
Cuts at: 4594 4594
Size: 5695
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5695
PfoI T'CCnGG_A
Cuts at: 2904 2904
Size: 5695
PmeI GTTT'AAAC
Cuts at: 2999 2999
Size: 5695
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2861 2893 3764 3796 5277 5309 2861
Size: 32 871 32 1481 32 3247
Fragments arranged by size:
3247 1481 871 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2493 2902 2493
Size: 409 5286
PshAI GACnn'nnGTC
Cuts at: 1613 2463 1613
Size: 850 4845
PsiI TTA'TAA
Cuts at: 3265 3265
Size: 5695
PspOMI G'GGCC_C
Cuts at: 2829 2829
Size: 5695
PstI C_TGCA'G
Cuts at: 830 2592 2649 2676 3047 830
Size: 1762 57 27 371 3478
Fragments arranged by size:
3478 1762 371 57 27
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5695
PvuII CAG'CTG
Cuts at: 2644 2644
Size: 5695
SacI G_AGCT'C
Cuts at: 2267 2267
Size: 5695
SalI G'TCGA_C
Cuts at: 1936 3037 1936
Size: 1101 4594
SbfI CC_TGCA'GG
Cuts at: 3047 3047
Size: 5695
ScaI AGT'ACT
Cuts at: 3943 5655 3943
Size: 1712 3983
SfcI C'TryA_G
Cuts at: 826 1045 2398 2588 2645 2672 3043 4178 4859
Size: 219 1353 190 57 27 371 1135 681
Cuts at: 4859 5050 826
Size: 191 1471
Fragments arranged by size:
1471 1353 1135 681 371 219 191 190
57 27
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5695
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5695
SmaI CCC'GGG
Cuts at: 1872 2834 3022 1872
Size: 962 188 4545
Fragments arranged by size:
4545 962 188
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3799 4700 4962 820
Size: 197 926 1856 901 262 1553
Fragments arranged by size:
1856 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3486 493
Size: 2993 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5695
SphI G_CATG'C
Cuts at: 1132 3053 1132
Size: 1921 3774
SrfI GCCC'GGGC
Cuts at: 2834 2834
Size: 5695
SspI AAT'ATT
Cuts at: 5 52 3619 5
Size: 47 3567 2081
Fragments arranged by size:
3567 2081 47
StuI AGG'CCT
Cuts at: 1517 2190 1517
Size: 673 5022
StyI C'CwwG_G
Cuts at: 513 1065 2004 3134 513
Size: 552 939 1130 3074
Fragments arranged by size:
3074 1130 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2998 3722 3739 3892 4077 4522 325
Size: 1255 1418 724 17 153 185 445 1498
Fragments arranged by size:
1498 1418 1255 724 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3941 5653 96
Size: 274 80 33 51 1443 1964 1712 138
Fragments arranged by size:
1964 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5655 5687 5655
Size: 32 5663
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5695
XbaI T'CTAG_A
Cuts at: 3031 3031
Size: 5695
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5695
XmaI C'CCGG_G
Cuts at: 1870 2832 3020 1870
Size: 962 188 4545
Fragments arranged by size:
4545 962 188
XmnI GAAnn'nnTTC
Cuts at: 3824 3824
Size: 5695
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3503 276
Size: 53 83 186 936 1969 2468
Fragments arranged by size:
2468 1969 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI
SfiI SfoI SwaI XcmI