Restriction map for
pFN21AB8647
(Circular) (Six-base) MAPSORT of: pFN21AB8647.seq Check: 7413 from: 1 to: 7177
pFN21AB8647 7177 bp
With 182 enzymes: SgfI *
August 16, 2010 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2432 2730 2432
Size: 298 6879
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4987 278
Size: 53 83 186 936 3451 2468
Fragments arranged by size:
3451 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3772 4428 4520 1937
Size: 1835 656 92 4594
Fragments arranged by size:
4594 1835 656 92
Acc65I G'GTAC_C
Cuts at: 4498 4498
Size: 7177
AclI AA'CG_TT
Cuts at: 1563 5304 5677 1563
Size: 3741 373 3063
Fragments arranged by size:
3741 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2702 5240 6624 6892 1632
Size: 1070 2538 1384 268 1917
Fragments arranged by size:
2538 1917 1384 1070 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6980
AflIII A'CryG_T
Cuts at: 1203 4175 6076 7053 1203
Size: 2972 1901 977 1327
Fragments arranged by size:
2972 1901 1327 977
AgeI A'CCGG_T
Cuts at: 6008 6008
Size: 7177
AhdI GACnn_n'nnGTC
Cuts at: 3386 5906 3386
Size: 2520 4657
AleI CACnn'nnGTG
Cuts at: 1166 2840 3780 4445 1166
Size: 1674 940 665 3898
Fragments arranged by size:
3898 1674 940 665
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3509 4217 1184
Size: 2325 708 4144
Fragments arranged by size:
4144 2325 708
AlwNI CAG_nnn'CTG
Cuts at: 2497 4237 4920 6492 2497
Size: 1740 683 1572 3182
Fragments arranged by size:
3182 1740 1572 683
ApaI G_GGCC'C
Cuts at: 2732 2817 3306 3695 3793 2732
Size: 85 489 389 98 6116
Fragments arranged by size:
6116 489 389 98 85
ApaLI G'TGCA_C
Cuts at: 3166 3317 5234 6390 3166
Size: 151 1917 1156 3953
Fragments arranged by size:
3953 1917 1156 151
ApoI r'AATT_y
Cuts at: 1493 2623 4486 4712 4864 6973 1493
Size: 1130 1863 226 152 2109 1697
Fragments arranged by size:
2109 1863 1697 1130 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5731 160
Size: 872 4699 1606
Fragments arranged by size:
4699 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7177
AvaI C'yCGr_G
Cuts at: 1870 1943 2118 2453 2480 2573 4502 1870
Size: 73 175 335 27 93 1929 4545
Fragments arranged by size:
4545 1929 335 175 93 73 27
AvrII C'CTAG_G
Cuts at: 2203 2203
Size: 7177
BamHI G'GATC_C
Cuts at: 3240 4458 4507 4874 3240
Size: 1218 49 367 5543
Fragments arranged by size:
5543 1218 367 49
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2137 2718 3081 3091 3562
Size: 325 217 627 350 581 363 10 471
Cuts at: 3562 4100 4115 4498 5953 618
Size: 538 15 383 1455 1842
Fragments arranged by size:
1842 1455 627 581 538 471 383 363
350 325 217 15 10
BanII G_rGCy'C
Cuts at: 1391 2352 2480 2732 2817 2868 3306 3695 3793
Size: 961 128 252 85 51 438 389 98
Cuts at: 3793 4071 4496 1391
Size: 278 425 4072
Fragments arranged by size:
4072 961 438 425 389 278 252 128
98 85 51
BbeI G_GCGC'C
Cuts at: 4104 4119 4104
Size: 15 7162
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2962 928
Size: 971 1063 5143
Fragments arranged by size:
5143 1063 971
BbvCI CC'TCA_GC
Cuts at: 3029 3029
Size: 7177
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5368 5402 1903
Size: 34 50 34 3347 34 3678
Fragments arranged by size:
3678 3347 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5069 6279 5069
Size: 1210 5967
BclI T'GATC_A
Cuts at: 1548 4449 1548
Size: 2901 4276
BfrBI ATG'CAT
Cuts at: 3486 3486
Size: 7177
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2900 3423 3690
Size: 107 122 71 631 605 1228 523 267
Cuts at: 3690 4124 5788 136
Size: 434 1664 1525
Fragments arranged by size:
1664 1525 1228 631 605 523 434 267
122 107 71
BglII A'GATC_T
Cuts at: 3288 7172 3288
Size: 3884 3293
BlpI GC'TnA_GC
Cuts at: 2474 4594 2474
Size: 2120 5057
Bme1580I G_kGCm'C
Cuts at: 1790 2142 2732 2817 3096 3170 3306 3321 3695
Size: 352 590 85 279 74 136 15 374
Cuts at: 3695 3793 5238 6394 1790
Size: 98 1445 1156 2573
Fragments arranged by size:
2573 1445 1156 590 374 352 279 136
98 85 74 15
BmgBI CAC'GTC
Cuts at: 1318 2806 3982 1318
Size: 1488 1176 4513
Fragments arranged by size:
4513 1488 1176
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4908 5866 442
Size: 416 53 83 399 24 3491 958 1753
Fragments arranged by size:
3491 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7177
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3380 3380
Size: 7177
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2371 2689 2780 2840 3375 3671 3682 3743
Size: 1132 318 91 60 535 296 11 61
Cuts at: 3743 3930 5837 1239
Size: 187 1907 2579
Fragments arranged by size:
2579 1907 1132 535 318 296 187 91
61 60 11
Bpu10I CC'TnA_GC
Cuts at: 3029 3029
Size: 7177
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2327 5302 6167 6465 2327
Size: 2975 865 298 3039
Fragments arranged by size:
3039 2975 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5840 882
Size: 617 4341 2219
Fragments arranged by size:
4341 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2656 4968 493
Size: 711 1452 2312 2702
Fragments arranged by size:
2702 2312 1452 711
BsaBI GATnn'nnATC
Cuts at: 3269 7171 3269
Size: 3902 3275
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2885 3024 4101 4116
Size: 53 83 186 936 1352 139 1077 15
Cuts at: 4116 4268 4425 4984 5366 6001 275
Size: 152 157 559 382 635 1451
Fragments arranged by size:
1451 1352 1077 936 635 559 382 186
157 152 139 83 53 15
BsaWI w'CCGG_w
Cuts at: 3597 5610 6008 6282 6429 6840 3597
Size: 2013 398 274 147 411 3934
Fragments arranged by size:
3934 2013 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2369 2375 2399 2405 2501
Size: 30 165 30 966 6 24 6 96
Cuts at: 2501 2531 2687 2717 3190 3220 3266 3296 1178
Size: 30 156 30 473 30 46 30 5059
Fragments arranged by size:
5059 966 473 165 156 96 46 30
30 30 30 30 30 24 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2522 3157 3680 1186
Size: 1336 635 523 4683
Fragments arranged by size:
4683 1336 635 523
BseYI C'CCAG_C
Cuts at: 2632 3047 3807 4095 4389 6380 6876 2632
Size: 415 760 288 294 1991 496 2933
Fragments arranged by size:
2933 1991 760 496 415 294 288
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2510 2909 2978 3929 4061 4238 4261
Size: 573 774 399 69 951 132 177 23
Cuts at: 4261 1163
Size: 4079
Fragments arranged by size:
4079 951 774 573 399 177 132 69
23
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2907 3261 4023 4627 5388 6416
Size: 275 186 906 354 762 604 761 1028
Cuts at: 6416 1540
Size: 2301
Fragments arranged by size:
2301 1028 906 762 761 604 354 275
186
BsiHKAI G_wGCw'C
Cuts at: 2352 2460 2952 3170 3321 3672 5238 5323 6394
Size: 108 492 218 151 351 1566 85 1071
Cuts at: 6394 2352
Size: 3135
Fragments arranged by size:
3135 1566 1071 492 351 218 151 108
85
BsmI GAATG_Cn'
Cuts at: 4688 4781 4688
Size: 93 7084
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2142 2352 2460 2480 2732 2817 2868
Size: 399 352 210 108 20 252 85 51
Cuts at: 2868 2952 3096 3170 3306 3321 3672 3695 3793
Size: 84 144 74 136 15 351 23 98
Cuts at: 3793 4071 4496 5238 5323 6394 1391
Size: 278 425 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 425 399 352 351 278
252 210 144 136 108 98 85 85
84 74 51 23 20 15
BspEI T'CCGG_A
Cuts at: 6840 6840
Size: 7177
BspHI T'CATG_A
Cuts at: 5066 6796 5066
Size: 1730 5447
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2432 2730 4532 844
Size: 1588 298 1802 3489
Fragments arranged by size:
3489 1802 1588 298
BsrBI CCG'CTC
Cuts at: 1394 1823 5064 1394
Size: 429 3241 3507
Fragments arranged by size:
3507 3241 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5672 5846 65
Size: 1183 4424 174 1396
Fragments arranged by size:
4424 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2463 2962 4286 4407 5821 6008 1700
Size: 110 653 499 1324 121 1414 187 2869
Fragments arranged by size:
2869 1414 1324 653 499 187 121 110
BsrGI T'GTAC_A
Cuts at: 96 3330 96
Size: 3234 3943
BssHII G'CGCG_C
Cuts at: 1925 4049 1925
Size: 2124 5053
BssSI C'ACGA_G
Cuts at: 2165 5237 6249 2165
Size: 3072 1012 3093
Fragments arranged by size:
3093 3072 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2497 794
Size: 1703 5474
BstBI TT'CG_AA
Cuts at: 5981 5981
Size: 7177
BstEII G'GTnAC_C
Cuts at: 1184 4471 1184
Size: 3287 3890
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2033 3427 2011
Size: 22 1394 5761
Fragments arranged by size:
5761 1394 22
BstYI r'GATC_y
Cuts at: 1972 2281 3240 3288 4458 4507 4874 5259 5276
Size: 309 959 48 1170 49 367 385 17
Cuts at: 5276 6728 6814 6826 7172 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1170 959 385 367 346 309
86 49 48 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2010 2868 3246 3255 3743 3855 513
Size: 552 945 858 378 9 488 112 3835
Fragments arranged by size:
3835 945 858 552 488 378 112 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3781 487
Size: 683 56 2555 3883
Fragments arranged by size:
3883 2555 683 56
BtsI GCAGTG_nn'
Cuts at: 2320 2495 3070 3491 3554 3632 3826 4078 4693
Size: 175 575 421 63 78 194 252 615
Cuts at: 4693 5487 5507 2320
Size: 794 20 3990
Fragments arranged by size:
3990 794 615 575 421 252 194 175
78 63 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7177
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7142
DraI TTT'AAA
Cuts at: 4481 4481
Size: 7177
DraIII CAC_nnn'GTG
Cuts at: 4009 4358 4009
Size: 349 6828
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6184 809
Size: 507 4868 1802
Fragments arranged by size:
4868 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2013 2793 2808
Size: 54 1425 50 275 18 183 780 15
Cuts at: 2808 3252 3258 3740 4020 4360 4405 4624 5513
Size: 444 6 482 280 340 45 219 889
Cuts at: 5513 7142 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 780 482 444 340 280
275 219 183 54 50 45 43 18
15 6
EagI C'GGCC_G
Cuts at: 1537 1812 3258 4020 4624 1537
Size: 275 1446 762 604 4090
Fragments arranged by size:
4090 1446 762 604 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2819 3537 3954 5107 1353
Size: 1466 718 417 1153 3423
Fragments arranged by size:
3423 1466 1153 718 417
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2120 2231 4142 5764 6138 6284
Size: 1064 138 531 111 1911 1622 374 146
Cuts at: 6284 387
Size: 1280
Fragments arranged by size:
1911 1622 1280 1064 531 374 146 138
111
EcoICRI GAG'CTC
Cuts at: 2350 2350
Size: 7177
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2371 2689 2702 2780 2840 3375 3671
Size: 393 739 318 13 78 60 535 296
Cuts at: 3671 3682 3743 3930 5240 5837 6624 6892 1239
Size: 11 61 187 1310 597 787 268 1524
Fragments arranged by size:
1524 1310 787 739 597 535 393 318
296 268 187 78 61 60 13 11
EcoNI CCTnn'n_nnAGG
Cuts at: 3881 3881
Size: 7177
EcoO109I rG'GnC_Cy
Cuts at: 2020 2414 2578 2671 2687 2728 2729 2737 2814
Size: 394 164 93 16 41 1 8 77
Cuts at: 2814 2920 3691 3692 3789 3790 4234 4393 2020
Size: 106 771 1 97 1 444 159 4804
Fragments arranged by size:
4804 771 444 394 164 159 106 97
93 77 41 16 8 1 1 1
EcoRI G'AATT_C
Cuts at: 2623 4486 4864 2623
Size: 1863 378 4936
Fragments arranged by size:
4936 1863 378
FseI GG_CCGG'CC
Cuts at: 4411 4411
Size: 7177
FspI TGC'GCA
Cuts at: 3215 5683 3215
Size: 2468 4709
HaeII r_GCGC'y
Cuts at: 1678 3989 4104 4119 6324 1678
Size: 2311 115 15 2205 2531
Fragments arranged by size:
2531 2311 2205 115 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2501 2533 3372 3404 5818
Size: 32 135 32 929 32 839 32 2414
Cuts at: 5818 5850 5892 5924 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2414 929 839 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2527 3773 4521 4767 669
Size: 988 281 589 1246 748 246 3079
Fragments arranged by size:
3079 1246 988 748 589 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7177
HpaI GTT'AAC
Cuts at: 4767 4767
Size: 7177
KasI G'GCGC_C
Cuts at: 4100 4115 4100
Size: 15 7162
KpnI G_GTAC'C
Cuts at: 4502 4502
Size: 7177
MfeI C'AATT_G
Cuts at: 4776 4776
Size: 7177
MluI A'CGCG_T
Cuts at: 7053 7053
Size: 7177
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3321 6291 6475 1305
Size: 2016 2970 184 2007
Fragments arranged by size:
2970 2016 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2795 3254 3742 10
Size: 54 1425 343 963 459 488 3445
Fragments arranged by size:
3445 1425 963 488 459 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2840 3463 3780 4445 5135 5494
Size: 648 843 831 623 317 665 690 359
Cuts at: 5494 5653 518
Size: 159 2042
Fragments arranged by size:
2042 843 831 690 665 648 623 359
317 159
MspA1I CmG'CkG
Cuts at: 1606 2709 3807 3821 4339 4365 4389 4455 4593
Size: 1103 1098 14 518 26 24 66 138
Cuts at: 4593 5270 6418 6663 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 1103 1098 677 518 245 138
66 26 24 14
NaeI GCC'GGC
Cuts at: 2465 4288 4409 2465
Size: 1823 121 5233
Fragments arranged by size:
5233 1823 121
NarI GG'CG_CC
Cuts at: 4101 4116 4101
Size: 15 7162
NcoI C'CATG_G
Cuts at: 513 1065 3743 513
Size: 552 2678 3947
Fragments arranged by size:
3947 2678 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7177
NgoMIV G'CCGG_C
Cuts at: 2463 4286 4407 2463
Size: 1823 121 5233
Fragments arranged by size:
5233 1823 121
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7177
NotI GC'GGCC_GC
Cuts at: 4624 4624
Size: 7177
NsiI A_TGCA'T
Cuts at: 3488 3488
Size: 7177
NspI r_CATG'y
Cuts at: 1132 1229 3486 4179 4279 4535 6080 1132
Size: 97 2257 693 100 256 1545 2229
Fragments arranged by size:
2257 2229 1545 693 256 100 97
PasI CC'CwG_GG
Cuts at: 1348 3019 3172 1348
Size: 1671 153 5353
Fragments arranged by size:
5353 1671 153
PciI A'CATG_T
Cuts at: 4175 6076 4175
Size: 1901 5276
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4009 4468 1108
Size: 2901 459 3817
Fragments arranged by size:
3817 2901 459
PfoI T'CCnGG_A
Cuts at: 2589 3834 2589
Size: 1245 5932
PmeI GTTT'AAAC
Cuts at: 4481 4481
Size: 7177
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2069 2101 5246 5278 6759 6791 2069
Size: 32 3145 32 1481 32 2455
Fragments arranged by size:
3145 2455 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2414 2671 2737 4234 2414
Size: 257 66 1497 5357
Fragments arranged by size:
5357 1497 257 66
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7177
PsiI TTA'TAA
Cuts at: 4747 4747
Size: 7177
PspOMI G'GGCC_C
Cuts at: 2728 2813 3302 3691 3789 2728
Size: 85 489 389 98 6116
Fragments arranged by size:
6116 489 389 98 85
PstI C_TGCA'G
Cuts at: 830 2727 2855 3225 3650 3996 4206 4529 830
Size: 1897 128 370 425 346 210 323 3478
Fragments arranged by size:
3478 1897 425 370 346 323 210 128
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7177
PvuII CAG'CTG
Cuts at: 2709 3821 4389 4455 2709
Size: 1112 568 66 5431
Fragments arranged by size:
5431 1112 568 66
SacI G_AGCT'C
Cuts at: 2352 2352
Size: 7177
SalI G'TCGA_C
Cuts at: 1936 3771 4519 1936
Size: 1835 748 4594
Fragments arranged by size:
4594 1835 748
SanDI GG'GwC_CC
Cuts at: 2414 2414
Size: 7177
SapI GCTCTTCn'nnn_
Cuts at: 3537 3537
Size: 7177
SbfI CC_TGCA'GG
Cuts at: 2727 4529 2727
Size: 1802 5375
ScaI AGT'ACT
Cuts at: 5425 7137 5425
Size: 1712 5465
SexAI A'CCwGG_T
Cuts at: 4260 4260
Size: 7177
SfcI C'TryA_G
Cuts at: 826 1045 2548 2647 2723 2851 3221 3646 3992
Size: 219 1503 99 76 128 370 425 346
Cuts at: 3992 4202 4525 5660 6341 6532 826
Size: 210 323 1135 681 191 1471
Fragments arranged by size:
1503 1471 1135 681 425 370 346 323
219 210 191 128 99 76
SfiI GGCCn_nnn'nGGCC
Cuts at: 3690 3690
Size: 7177
SfoI GGC'GCC
Cuts at: 4102 4117 4102
Size: 15 7162
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7177
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7177
SmaI CCC'GGG
Cuts at: 1872 2120 4504 1872
Size: 248 2384 4545
Fragments arranged by size:
4545 2384 248
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2342 5281 6182 6444 820
Size: 197 926 399 2939 901 262 1553
Fragments arranged by size:
2939 1553 926 901 399 262 197
SnaBI TAC'GTA
Cuts at: 493 4968 493
Size: 4475 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7177
SphI G_CATG'C
Cuts at: 1132 4279 4535 1132
Size: 3147 256 3774
Fragments arranged by size:
3774 3147 256
SspI AAT'ATT
Cuts at: 5 52 5101 5
Size: 47 5049 2081
Fragments arranged by size:
5049 2081 47
StuI AGG'CCT
Cuts at: 1517 2093 2978 1517
Size: 576 885 5716
Fragments arranged by size:
5716 885 576
StyI C'CwwG_G
Cuts at: 513 1065 2203 2733 3498 3743 4162 4616 513
Size: 552 1138 530 765 245 419 454 3074
Fragments arranged by size:
3074 1138 765 552 530 454 419 245
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2802 3185 3799 5204 5221 5374 5559
Size: 1255 1222 383 614 1405 17 153 185
Cuts at: 5559 6004 325
Size: 445 1498
Fragments arranged by size:
1498 1405 1255 1222 614 445 383 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2238 2257 3330
Size: 274 80 33 51 1443 261 19 1073
Cuts at: 3330 5423 7135 96
Size: 2093 1712 138
Fragments arranged by size:
2093 1712 1443 1073 274 261 138 80
51 33 19
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2687 2719 3190 3222 7137 7169 2687
Size: 32 471 32 3915 32 2695
Fragments arranged by size:
3915 2695 471 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7177
XbaI T'CTAG_A
Cuts at: 4513 4513
Size: 7177
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2365 2803 2365
Size: 438 6739
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7177
XmaI C'CCGG_G
Cuts at: 1870 2118 4502 1870
Size: 248 2384 4545
Fragments arranged by size:
4545 2384 248
XmnI GAAnn'nnTTC
Cuts at: 5306 5306
Size: 7177
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4985 276
Size: 53 83 186 936 3451 2468
Fragments arranged by size:
3451 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BsiWI BsmBI BstZ17I Bsu36I
EcoRV FalI FspAI NruI PacI PmlI PspXI PsrI
RsrII SacII SrfI SwaI