Restriction map for
pFN21AB8645
(Circular) (Six-base) MAPSORT of: pFN21AB8645.seq Check: 9880 from: 1 to: 5503
pFN21AB8645 5503 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3313 278
Size: 53 83 186 936 1777 2468
Fragments arranged by size:
2468 1777 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2181 2846 1937
Size: 244 665 4594
Fragments arranged by size:
4594 665 244
Acc65I G'GTAC_C
Cuts at: 2824 2824
Size: 5503
AclI AA'CG_TT
Cuts at: 1563 3630 4003 1563
Size: 2067 373 3063
Fragments arranged by size:
3063 2067 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2324 2393 2548 3566 4950 5218 1632
Size: 692 69 155 1018 1384 268 1917
Fragments arranged by size:
1917 1384 1018 692 268 155 69
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5306
AflIII A'CryG_T
Cuts at: 1203 2199 4402 5379 1203
Size: 996 2203 977 1327
Fragments arranged by size:
2203 1327 996 977
AgeI A'CCGG_T
Cuts at: 4334 4334
Size: 5503
AhdI GACnn_n'nnGTC
Cuts at: 4232 4232
Size: 5503
AleI CACnn'nnGTG
Cuts at: 1166 2537 1166
Size: 1371 4132
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2090 1184
Size: 906 4597
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2534 2566 2534
Size: 32 5471
AlwNI CAG_nnn'CTG
Cuts at: 2353 3246 4818 2353
Size: 893 1572 3038
Fragments arranged by size:
3038 1572 893
ApaLI G'TGCA_C
Cuts at: 2791 3560 4716 2791
Size: 769 1156 3578
Fragments arranged by size:
3578 1156 769
ApoI r'AATT_y
Cuts at: 1493 2812 3038 3190 5299 1493
Size: 1319 226 152 2109 1697
Fragments arranged by size:
2109 1697 1319 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4057 160
Size: 872 3025 1606
Fragments arranged by size:
3025 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5503
AvaI C'yCGr_G
Cuts at: 1870 1943 2088 2828 1870
Size: 73 145 740 4545
Fragments arranged by size:
4545 740 145 73
BamHI G'GATC_C
Cuts at: 2833 3200 2833
Size: 367 5136
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2081 2455 2824 4279 618
Size: 325 217 627 294 374 369 1455 1842
Fragments arranged by size:
1842 1455 627 374 369 325 294 217
BanII G_rGCy'C
Cuts at: 1391 2105 2597 2822 1391
Size: 714 492 225 4072
Fragments arranged by size:
4072 714 492 225
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2018 2275 928
Size: 971 119 257 4156
Fragments arranged by size:
4156 971 257 119
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3694 3728 1903
Size: 34 1757 34 3678
Fragments arranged by size:
3678 1757 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2215 3395 4605 2215
Size: 1180 1210 3113
Fragments arranged by size:
3113 1210 1180
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5503
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4114 136
Size: 107 122 71 631 605 2442 1525
Fragments arranged by size:
2442 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2613 5498 2613
Size: 2885 2618
BlpI GC'TnA_GC
Cuts at: 2920 2920
Size: 5503
Bme1580I G_kGCm'C
Cuts at: 1790 2049 2460 2795 3564 4720 1790
Size: 259 411 335 769 1156 2573
Fragments arranged by size:
2573 1156 769 411 335 259
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5503
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3234 4192 442
Size: 416 53 83 399 24 1817 958 1753
Fragments arranged by size:
1817 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5503
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2375 4163 1239
Size: 1136 1788 2579
Fragments arranged by size:
2579 1788 1136
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2720 3628 4493 4791 2720
Size: 908 865 298 3432
Fragments arranged by size:
3432 908 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2701 4166 882
Size: 617 1202 1465 2219
Fragments arranged by size:
2219 1465 1202 617
BsaAI yAC'GTr
Cuts at: 493 1204 2557 3294 493
Size: 711 1353 737 2702
Fragments arranged by size:
2702 1353 737 711
BsaBI GATnn'nnATC
Cuts at: 5497 5497
Size: 5503
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2634 2682 3310 3692
Size: 53 83 186 936 1101 48 628 382
Cuts at: 3692 4327 275
Size: 635 1451
Fragments arranged by size:
1451 1101 936 635 628 382 186 83
53 48
BsaWI w'CCGG_w
Cuts at: 3936 4334 4608 4755 5166 3936
Size: 398 274 147 411 4273
Fragments arranged by size:
4273 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2348 2378 1178
Size: 30 165 30 945 30 4303
Fragments arranged by size:
4303 945 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5503
BseYI C'CCAG_C
Cuts at: 2077 2717 4706 5202 2077
Size: 640 1989 496 2378
Fragments arranged by size:
2378 1989 640 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2017 2642 1163
Size: 573 281 625 4024
Fragments arranged by size:
4024 625 573 281
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2953 3714 4742 1540
Size: 275 186 952 761 1028 2301
Fragments arranged by size:
2301 1028 952 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2105 2562 2795 3564 3649 4720 2105
Size: 457 233 769 85 1071 2888
Fragments arranged by size:
2888 1071 769 457 233 85
BsmI GAATG_Cn'
Cuts at: 3014 3107 3014
Size: 93 5410
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2049 2105 2460 2562 2597 2795 2822
Size: 399 259 56 355 102 35 198 27
Cuts at: 2822 3564 3649 4720 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 355 259 198 102
85 56 35 27
BspEI T'CCGG_A
Cuts at: 5166 5166
Size: 5503
BspHI T'CATG_A
Cuts at: 3392 5122 3392
Size: 1730 3773
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2858 844
Size: 2014 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3390 1394
Size: 429 1567 3507
Fragments arranged by size:
3507 1567 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3998 4172 65
Size: 1183 2750 174 1396
Fragments arranged by size:
2750 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4147 4334 1700
Size: 110 2337 187 2869
Fragments arranged by size:
2869 2337 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5503
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5503
BssSI C'ACGA_G
Cuts at: 2585 3563 4575 2585
Size: 978 1012 3513
Fragments arranged by size:
3513 1012 978
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5503
BstBI TT'CG_AA
Cuts at: 4307 4307
Size: 5503
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5503
BstYI r'GATC_y
Cuts at: 1972 2349 2613 2833 3200 3585 3602 5054 5140
Size: 377 264 220 367 385 17 1452 86
Cuts at: 5140 5152 5498 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 377 367 346 264 220
86 17 12
BstZ17I GTA'TAC
Cuts at: 2182 2182
Size: 5503
BtgI C'CryG_G
Cuts at: 513 1065 2191 2406 2434 513
Size: 552 1126 215 28 3582
Fragments arranged by size:
3582 1126 552 215 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2591 487
Size: 683 56 1365 3399
Fragments arranged by size:
3399 1365 683 56
BtsI GCAGTG_nn'
Cuts at: 3019 3813 3833 3019
Size: 794 20 4689
Fragments arranged by size:
4689 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5503
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5468
DraI TTT'AAA
Cuts at: 2807 2807
Size: 5503
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4510 809
Size: 507 3194 1802
Fragments arranged by size:
3194 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2128 2520 2571
Size: 54 1425 50 275 18 298 392 51
Cuts at: 2571 2950 3839 5468 8
Size: 379 889 1629 43
Fragments arranged by size:
1629 1425 889 392 379 298 275 54
51 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2950 1537
Size: 275 1138 4090
Fragments arranged by size:
4090 1138 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2567 3433 1353
Size: 1214 866 3423
Fragments arranged by size:
3423 1214 866
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2411 4090 4464 4610 387
Size: 1064 138 822 1679 374 146 1280
Fragments arranged by size:
1679 1280 1064 822 374 146 138
EcoICRI GAG'CTC
Cuts at: 2103 2103
Size: 5503
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2324 2375 2393 2548 3566 4163 4950
Size: 393 692 51 18 155 1018 597 787
Cuts at: 4950 5218 1239
Size: 268 1524
Fragments arranged by size:
1524 1018 787 692 597 393 268 155
51 18
EcoO109I rG'GnC_Cy
Cuts at: 2649 2755 2649
Size: 106 5397
EcoRI G'AATT_C
Cuts at: 2812 3190 2812
Size: 378 5125
FspI TGC'GCA
Cuts at: 2255 4009 2255
Size: 1754 3749
HaeII r_GCGC'y
Cuts at: 1678 2338 4650 1678
Size: 660 2312 2531
Fragments arranged by size:
2531 2312 660
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2348 2380 4144 4176 4218
Size: 32 135 32 776 32 1764 32 42
Cuts at: 4218 4250 1373
Size: 32 2626
Fragments arranged by size:
2626 1764 776 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2725 2847 3093 669
Size: 988 281 787 122 246 3079
Fragments arranged by size:
3079 988 787 281 246 122
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5503
HpaI GTT'AAC
Cuts at: 3093 3093
Size: 5503
KpnI G_GTAC'C
Cuts at: 2828 2828
Size: 5503
MfeI C'AATT_G
Cuts at: 3102 3102
Size: 5503
MluI A'CGCG_T
Cuts at: 5379 5379
Size: 5503
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4617 4801 1305
Size: 3312 184 2007
Fragments arranged by size:
3312 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2573 10
Size: 54 1425 343 741 2940
Fragments arranged by size:
2940 1425 741 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2072 2291 2537 3461 3820 3979 518
Size: 648 906 219 246 924 359 159 2042
Fragments arranged by size:
2042 924 906 648 359 246 219 159
MspA1I CmG'CkG
Cuts at: 1606 2025 2193 2919 3596 4744 4989 1606
Size: 419 168 726 677 1148 245 2120
Fragments arranged by size:
2120 1148 726 677 419 245 168
NcoI C'CATG_G
Cuts at: 513 1065 2406 513
Size: 552 1341 3610
Fragments arranged by size:
3610 1341 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5503
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5503
NotI GC'GGCC_GC
Cuts at: 2950 2950
Size: 5503
NspI r_CATG'y
Cuts at: 1132 1229 2203 2662 2861 4406 1132
Size: 97 974 459 199 1545 2229
Fragments arranged by size:
2229 1545 974 459 199 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5503
PciI A'CATG_T
Cuts at: 2199 4402 2199
Size: 2203 3300
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5503
PfoI T'CCnGG_A
Cuts at: 2217 2352 2217
Size: 135 5368
PmeI GTTT'AAAC
Cuts at: 2807 2807
Size: 5503
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3572 3604 5085 5117 3572
Size: 32 1481 32 3958
Fragments arranged by size:
3958 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2755 2755
Size: 5503
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5503
PsiI TTA'TAA
Cuts at: 3073 3073
Size: 5503
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2489 2521 2489
Size: 32 5471
PstI C_TGCA'G
Cuts at: 830 2855 830
Size: 2025 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5503
SacI G_AGCT'C
Cuts at: 2105 2105
Size: 5503
SacII CC_GC'GG
Cuts at: 2194 2194
Size: 5503
SalI G'TCGA_C
Cuts at: 1936 2845 1936
Size: 909 4594
SapI GCTCTTCn'nnn_
Cuts at: 2567 2567
Size: 5503
SbfI CC_TGCA'GG
Cuts at: 2855 2855
Size: 5503
ScaI AGT'ACT
Cuts at: 3751 5463 3751
Size: 1712 3791
SfcI C'TryA_G
Cuts at: 826 1045 2851 3986 4667 4858 826
Size: 219 1806 1135 681 191 1471
Fragments arranged by size:
1806 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5503
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5503
SmaI CCC'GGG
Cuts at: 1872 2830 1872
Size: 958 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2735 3607 4508 4770 820
Size: 197 926 792 872 901 262 1553
Fragments arranged by size:
1553 926 901 872 792 262 197
SnaBI TAC'GTA
Cuts at: 493 3294 493
Size: 2801 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5503
SphI G_CATG'C
Cuts at: 1132 2662 2861 1132
Size: 1530 199 3774
Fragments arranged by size:
3774 1530 199
SspI AAT'ATT
Cuts at: 5 52 3427 5
Size: 47 3375 2081
Fragments arranged by size:
3375 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5503
StyI C'CwwG_G
Cuts at: 513 1065 2385 2406 2751 2942 513
Size: 552 1320 21 345 191 3074
Fragments arranged by size:
3074 1320 552 345 191 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2069 2167 3530 3547 3700 3885 4330
Size: 1255 489 98 1363 17 153 185 445
Cuts at: 4330 325
Size: 1498
Fragments arranged by size:
1498 1363 1255 489 445 185 153 98
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2145 2739 3749
Size: 274 80 33 51 1443 168 594 1010
Cuts at: 3749 5461 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1010 594 274 168 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5463 5495 5463
Size: 32 5471
Tth111I GACn'n_nGTC
Cuts at: 1316 2017 1316
Size: 701 4802
XbaI T'CTAG_A
Cuts at: 2839 2839
Size: 5503
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5503
XmaI C'CCGG_G
Cuts at: 1870 2828 1870
Size: 958 4545
XmnI GAAnn'nnTTC
Cuts at: 3632 3632
Size: 5503
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3311 276
Size: 53 83 186 936 1777 2468
Fragments arranged by size:
2468 1777 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI
PstI PvuI SacI SacII SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PvuII
RsrII SanDI SexAI SfiI SfoI SrfI SwaI XcmI