Restriction map for
pFN21AB8641
(Circular) (Six-base) MAPSORT of: pFN21AB8641.seq Check: 2273 from: 1 to: 7282
pFN21AB8641 7282 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2686 4395 2686
Size: 1709 5573
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5092 278
Size: 53 83 186 936 3556 2468
Fragments arranged by size:
3556 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4625 1937
Size: 2688 4594
Acc65I G'GTAC_C
Cuts at: 4603 4603
Size: 7282
AclI AA'CG_TT
Cuts at: 1563 5409 5782 1563
Size: 3846 373 3063
Fragments arranged by size:
3846 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2352 2571 2898 4388 5345 6729 6997 1632
Size: 720 219 327 1490 957 1384 268 1917
Fragments arranged by size:
1917 1490 1384 957 720 327 268 219
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7085
AflIII A'CryG_T
Cuts at: 1203 4302 6181 7158 1203
Size: 3099 1879 977 1327
Fragments arranged by size:
3099 1879 1327 977
AgeI A'CCGG_T
Cuts at: 6113 6113
Size: 7282
AhdI GACnn_n'nnGTC
Cuts at: 6011 6011
Size: 7282
AleI CACnn'nnGTG
Cuts at: 1166 3539 4026 1166
Size: 2373 487 4422
Fragments arranged by size:
4422 2373 487
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3243 1184
Size: 2059 5223
AlwNI CAG_nnn'CTG
Cuts at: 4274 4402 5025 6597 4274
Size: 128 623 1572 4959
Fragments arranged by size:
4959 1572 623 128
ApaLI G'TGCA_C
Cuts at: 5339 6495 5339
Size: 1156 6126
ApoI r'AATT_y
Cuts at: 1493 2054 2354 4591 4817 4969 7078 1493
Size: 561 300 2237 226 152 2109 1697
Fragments arranged by size:
2237 2109 1697 561 300 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3356 5836 160
Size: 872 2324 2480 1606
Fragments arranged by size:
2480 2324 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7282
AvaI C'yCGr_G
Cuts at: 1870 1943 3192 3948 4041 4607 1870
Size: 73 1249 756 93 566 4545
Fragments arranged by size:
4545 1249 756 566 93 73
BamHI G'GATC_C
Cuts at: 4612 4979 4612
Size: 367 6915
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2521 2674 3402 4603 6058
Size: 325 217 627 734 153 728 1201 1455
Cuts at: 6058 618
Size: 1842
Fragments arranged by size:
1842 1455 1201 734 728 627 325 217
153
BanII G_rGCy'C
Cuts at: 1391 3459 3822 4601 1391
Size: 2068 363 779 4072
Fragments arranged by size:
4072 2068 779 363
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2543 3921 4305 4529 928
Size: 971 644 1378 384 224 3681
Fragments arranged by size:
3681 1378 971 644 384 224
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5473 5507 1903
Size: 34 3536 34 3678
Fragments arranged by size:
3678 3536 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3771 4440 5174 6384 3771
Size: 669 734 1210 4669
Fragments arranged by size:
4669 1210 734 669
BclI T'GATC_A
Cuts at: 1548 2560 3036 4092 1548
Size: 1012 476 1056 4738
Fragments arranged by size:
4738 1056 1012 476
BfrBI ATG'CAT
Cuts at: 2894 2894
Size: 7282
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5893 136
Size: 107 122 71 631 605 4221 1525
Fragments arranged by size:
4221 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7277 7277
Size: 7282
BlpI GC'TnA_GC
Cuts at: 3453 4699 3453
Size: 1246 6036
Bme1580I G_kGCm'C
Cuts at: 1790 2526 5343 6499 1790
Size: 736 2817 1156 2573
Fragments arranged by size:
2817 2573 1156 736
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7282
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2928 5013 5971
Size: 416 53 83 399 24 1511 2085 958
Cuts at: 5971 442
Size: 1753
Fragments arranged by size:
2085 1753 1511 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7282
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2185 2399 2847 2988 3321 3914 4301 5942
Size: 946 214 448 141 333 593 387 1641
Cuts at: 5942 1239
Size: 2579
Fragments arranged by size:
2579 1641 946 593 448 387 333 214
141
Bpu10I CC'TnA_GC
Cuts at: 2373 3398 2373
Size: 1025 6257
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4106 5407 6272 6570 4106
Size: 1301 865 298 4818
Fragments arranged by size:
4818 1301 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2185 2825 3692 5945 882
Size: 617 686 640 867 2253 2219
Fragments arranged by size:
2253 2219 867 686 640 617
BsaAI yAC'GTr
Cuts at: 493 1204 4001 5073 493
Size: 711 2797 1072 2702
Fragments arranged by size:
2797 2702 1072 711
BsaBI GATnn'nnATC
Cuts at: 7276 7276
Size: 7282
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5089 5471 6106 275
Size: 53 83 186 936 3556 382 635 1451
Fragments arranged by size:
3556 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2413 3426 5715 6113 6387 6534 6945 2413
Size: 1013 2289 398 274 147 411 2750
Fragments arranged by size:
2750 2289 1013 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2261 2291 2961 2991 3597
Size: 30 165 30 858 30 670 30 606
Cuts at: 3597 3627 3779 3809 1178
Size: 30 152 30 4651
Fragments arranged by size:
4651 858 670 606 165 152 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2273 2276 2279 2282 2790 3089 3812 1186
Size: 1087 3 3 3 508 299 723 4656
Fragments arranged by size:
4656 1087 723 508 299 3 3 3
BseYI C'CCAG_C
Cuts at: 2420 4395 6485 6981 2420
Size: 1975 2090 496 2721
Fragments arranged by size:
2721 2090 1975 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2628 4175 4229 1163
Size: 573 892 1547 54 4216
Fragments arranged by size:
4216 1547 892 573 54
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4732 5493 6521 1540
Size: 275 186 2731 761 1028 2301
Fragments arranged by size:
2731 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2510 2623 3459 5343 5428 6499 2510
Size: 113 836 1884 85 1071 3293
Fragments arranged by size:
3293 1884 1071 836 113 85
BsmI GAATG_Cn'
Cuts at: 2794 4793 4886 2794
Size: 1999 93 5190
Fragments arranged by size:
5190 1999 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3680 3680
Size: 7282
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2510 2526 2623 3459 3822 4601 5343
Size: 399 720 16 97 836 363 779 742
Cuts at: 5343 5428 6499 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 836 779 742 720 399 363
97 85 16
BspEI T'CCGG_A
Cuts at: 2413 6945 2413
Size: 4532 2750
BspHI T'CATG_A
Cuts at: 2557 5171 6901 2557
Size: 2614 1730 2938
Fragments arranged by size:
2938 2614 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2686 4395 4637 844
Size: 1842 1709 242 3489
Fragments arranged by size:
3489 1842 1709 242
BsrBI CCG'CTC
Cuts at: 1394 1823 5169 1394
Size: 429 3346 3507
Fragments arranged by size:
3507 3346 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2894 5777 5951 65
Size: 1183 1646 2883 174 1396
Fragments arranged by size:
2883 1646 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2608 3342 5926 6113 1700
Size: 110 798 734 2584 187 2869
Fragments arranged by size:
2869 2584 798 734 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7282
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7282
BssSI C'ACGA_G
Cuts at: 5342 6354 5342
Size: 1012 6270
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3301 4274 794
Size: 2507 973 3802
Fragments arranged by size:
3802 2507 973
BstBI TT'CG_AA
Cuts at: 6086 6086
Size: 7282
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7282
BstXI CCAn_nnnn'nTGG
Cuts at: 3589 4028 3589
Size: 439 6843
BstYI r'GATC_y
Cuts at: 1972 2368 3705 4612 4979 5364 5381 6833 6919
Size: 396 1337 907 367 385 17 1452 86
Cuts at: 6919 6931 7277 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1337 907 396 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4083 4083
Size: 7282
BtgI C'CryG_G
Cuts at: 513 1065 3678 513
Size: 552 2613 4117
Fragments arranged by size:
4117 2613 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6543
Fragments arranged by size:
6543 683 56
BtsI GCAGTG_nn'
Cuts at: 2508 2780 2927 4798 5592 5612 2508
Size: 272 147 1871 794 20 4178
Fragments arranged by size:
4178 1871 794 272 147 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7282
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3921 3956 529
Size: 35 3357 35 3855
Fragments arranged by size:
3855 3357 35 35
DraI TTT'AAA
Cuts at: 4586 4586
Size: 7282
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6289 809
Size: 507 4973 1802
Fragments arranged by size:
4973 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3201 3234 4729
Size: 54 1425 50 275 18 1371 33 1495
Cuts at: 4729 5618 7247 8
Size: 889 1629 43
Fragments arranged by size:
1629 1495 1425 1371 889 275 54 50
43 33 18
EagI C'GGCC_G
Cuts at: 1537 1812 4729 1537
Size: 275 2917 4090
Fragments arranged by size:
4090 2917 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2213 2250 2777 5212 1353
Size: 860 37 527 2435 3423
Fragments arranged by size:
3423 2435 860 527 37
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5869 6243 6389 387
Size: 1064 138 4280 374 146 1280
Fragments arranged by size:
4280 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3457 3457
Size: 7282
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2185 2352 2399 2571 2847 2898 2988
Size: 393 553 167 47 172 276 51 90
Cuts at: 2988 3321 3914 4301 4388 5345 5942 6729 6997
Size: 333 593 387 87 957 597 787 268
Cuts at: 6997 1239
Size: 1524
Fragments arranged by size:
1524 957 787 597 593 553 393 387
333 276 268 172 167 90 87 51
47
EcoO109I rG'GnC_Cy
Cuts at: 2590 3023 3994 2590
Size: 433 971 5878
Fragments arranged by size:
5878 971 433
EcoRI G'AATT_C
Cuts at: 4591 4969 4591
Size: 378 6904
EcoRV GAT'ATC
Cuts at: 3838 4138 3838
Size: 300 6982
FspI TGC'GCA
Cuts at: 5788 5788
Size: 7282
HaeII r_GCGC'y
Cuts at: 1678 3138 6429 1678
Size: 1460 3291 2531
Fragments arranged by size:
3291 2531 1460
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2009 2041 2261 2293 2417
Size: 32 135 32 437 32 220 32 124
Cuts at: 2417 2449 2846 2878 2961 2993 3091 3123 3175
Size: 32 397 32 83 32 98 32 52
Cuts at: 3175 3207 3745 3777 5923 5955 5997 6029 1373
Size: 32 538 32 2146 32 42 32 2626
Fragments arranged by size:
2626 2146 538 437 397 220 135 124
98 83 52 42 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4626 4872 669
Size: 988 281 2688 246 3079
Fragments arranged by size:
3079 2688 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7282
HpaI GTT'AAC
Cuts at: 4872 4872
Size: 7282
KpnI G_GTAC'C
Cuts at: 4607 4607
Size: 7282
MfeI C'AATT_G
Cuts at: 4881 4881
Size: 7282
MluI A'CGCG_T
Cuts at: 7158 7158
Size: 7282
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2472 3102 4322 6396 6580 1305
Size: 1167 630 1220 2074 184 2007
Fragments arranged by size:
2074 2007 1220 1167 630 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3236 10
Size: 54 1425 343 1404 4056
Fragments arranged by size:
4056 1425 1404 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2753 3225 3539 4026 4172 5240 5599
Size: 648 1587 472 314 487 146 1068 359
Cuts at: 5599 5758 518
Size: 159 2042
Fragments arranged by size:
2042 1587 1068 648 487 472 359 314
159 146
MspA1I CmG'CkG
Cuts at: 1606 3466 4274 4698 5375 6523 6768 1606
Size: 1860 808 424 677 1148 245 2120
Fragments arranged by size:
2120 1860 1148 808 677 424 245
NaeI GCC'GGC
Cuts at: 3344 3344
Size: 7282
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6730
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7282
NgoMIV G'CCGG_C
Cuts at: 3342 3342
Size: 7282
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7282
NotI GC'GGCC_GC
Cuts at: 4729 4729
Size: 7282
NsiI A_TGCA'T
Cuts at: 2896 2896
Size: 7282
NspI r_CATG'y
Cuts at: 1132 1229 3860 3980 4306 4640 6185 1132
Size: 97 2631 120 326 334 1545 2229
Fragments arranged by size:
2631 2229 1545 334 326 120 97
PacI TTA_AT'TAA
Cuts at: 3360 3360
Size: 7282
PasI CC'CwG_GG
Cuts at: 1348 2386 1348
Size: 1038 6244
PciI A'CATG_T
Cuts at: 4302 6181 4302
Size: 1879 5403
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7282
PmeI GTTT'AAAC
Cuts at: 4586 4586
Size: 7282
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5351 5383 6864 6896 5351
Size: 32 1481 32 5737
Fragments arranged by size:
5737 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2590 3994 2590
Size: 1404 5878
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7282
PsiI TTA'TAA
Cuts at: 4852 4852
Size: 7282
PspXI vC'TCGA_Gb
Cuts at: 4041 4041
Size: 7282
PstI C_TGCA'G
Cuts at: 830 4634 830
Size: 3804 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7282
PvuII CAG'CTG
Cuts at: 3466 4274 3466
Size: 808 6474
SacI G_AGCT'C
Cuts at: 3459 3459
Size: 7282
SalI G'TCGA_C
Cuts at: 1936 4624 1936
Size: 2688 4594
SbfI CC_TGCA'GG
Cuts at: 4634 4634
Size: 7282
ScaI AGT'ACT
Cuts at: 5530 7242 5530
Size: 1712 5570
SexAI A'CCwGG_T
Cuts at: 2442 3990 2442
Size: 1548 5734
SfcI C'TryA_G
Cuts at: 826 1045 4630 5765 6446 6637 826
Size: 219 3585 1135 681 191 1471
Fragments arranged by size:
3585 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7282
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7282
SmaI CCC'GGG
Cuts at: 1872 4609 1872
Size: 2737 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3192 4041 4121 5386 6287 6549
Size: 197 926 1249 849 80 1265 901 262
Cuts at: 6549 820
Size: 1553
Fragments arranged by size:
1553 1265 1249 926 901 849 262 197
80
SnaBI TAC'GTA
Cuts at: 493 5073 493
Size: 4580 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7282
SphI G_CATG'C
Cuts at: 1132 3860 4640 1132
Size: 2728 780 3774
Fragments arranged by size:
3774 2728 780
SspI AAT'ATT
Cuts at: 5 52 5206 5
Size: 47 5154 2081
Fragments arranged by size:
5154 2081 47
StuI AGG'CCT
Cuts at: 1517 4059 1517
Size: 2542 4740
StyI C'CwwG_G
Cuts at: 513 1065 2517 2670 2704 3648 4721 513
Size: 552 1452 153 34 944 1073 3074
Fragments arranged by size:
3074 1452 1073 944 552 153 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2239 2662 5309 5326 5479 5664 6109
Size: 1255 659 423 2647 17 153 185 445
Cuts at: 6109 325
Size: 1498
Fragments arranged by size:
2647 1498 1255 659 445 423 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3741 4568 5528
Size: 274 80 33 51 1443 1764 827 960
Cuts at: 5528 7240 96
Size: 1712 138
Fragments arranged by size:
1764 1712 1443 960 827 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2667 2699 4376 4408 7242 7274 2667
Size: 32 1677 32 2834 32 2675
Fragments arranged by size:
2834 2675 1677 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7282
XbaI T'CTAG_A
Cuts at: 4618 4618
Size: 7282
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3196 3345 3535 3196
Size: 149 190 6943
Fragments arranged by size:
6943 190 149
XhoI C'TCGA_G
Cuts at: 1943 3192 4041 1943
Size: 1249 849 5184
Fragments arranged by size:
5184 1249 849
XmaI C'CCGG_G
Cuts at: 1870 4607 1870
Size: 2737 4545
XmnI GAAnn'nnTTC
Cuts at: 3731 5411 3731
Size: 1680 5602
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5090 276
Size: 53 83 186 936 3556 2468
Fragments arranged by size:
3556 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PacI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI
PstI PvuI PvuII SacI SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI BsiWI BstZ17I DraIII EcoNI FalI FseI FspAI
KasI NarI NruI PfoI PmlI PspOMI PsrI RsrII
SacII SanDI SapI SfiI SfoI SrfI SwaI